Labshake search
Citations for Worthington Biochemical :
1 - 50 of 428 citations for HLA A*0201 NY ESO 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: ... and embedded in Paraplast (Polyscience, Inc, Worthington, NY). The blocks were sectioned at 8 μm ...
-
bioRxiv - Immunology 2024Quote: ... Enzymatic digestion was stopped with 1 mL of 10X hi ovomucoid inhibitor solution and 20 µL 0.4% DNase (Worthington, #LS002007) diluted in 10 mL inhibitor stock solution (ISS) ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Immunology 2023Quote: ... were digested with 2mg/mL type IV collagenase (Worthington, NY) to obtain single cell suspensions and the lymphocytes were enriched by Percoll gradient centrifugation as describes previously 11 ...
-
bioRxiv - Physiology 2022Quote: Human adipocytes were isolated from adipose tissue samples by collagenase (type 1, Worthington, NJ, USA) as described previously [24] ...
-
bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
-
bioRxiv - Neuroscience 2020Quote: ... hippocampi were later incubated twice for 15 minutes at 37°C with 100 units of papain (Worthington, NY) in DM and rinsed three times in DM and subsequently three times in plating medium (MEM ...
-
bioRxiv - Neuroscience 2020Quote: Primary human cortical tissue was dissociated with Papain (Worthington)-containing DNase ...
-
bioRxiv - Cell Biology 2023Quote: ... were isolated from ventricles of 8-month-old adult FVB hearts (Taconic, Germantown, NY) by retrograde heart perfusion with collagenase type II (Worthington Biochemical Corp). ACM were allowed to settle by gravity in the washing buffer with 2% BSA and stepwise [Ca2+] increase from 20 μM to 1.0 mM ...
-
bioRxiv - Neuroscience 2023Quote: ... Human samples were dissociated according to a modified papain-based protocol (Worthington), then filtered through a 40 µM filter before counting and dilution for scRNAseq.
-
bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
-
bioRxiv - Pathology 2021Quote: ... Retained proteins were digested with trypsin (Worthington), at a ratio of 10:1 protein to trypsin ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with trypsin (Worthington-Biochem) in 50 mM TEAB pH 8.0 at an enzyme-to-protein ratio of 1:10 (w/w ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were digested by adding bovine trypsin (Worthington-LS003750) and endoproteinase Lys-C (NEB-P8109S ...
Product cited:
Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl...Cat# LS003750, 100 mg, $170.00 Ask -
bioRxiv - Immunology 2019Quote: Human Omentum was weighed and 0.035 – 1.8g of tissue was digested using 2mg/ml Collagenase I (Worthington) in PBS (Invitrogen/sigma ...
-
bioRxiv - Immunology 2022Quote: ... isolated cells were cultured for 24 h with ovalbumin protein (Worthington, LS003048) at 500 μg/ml ...
-
bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Paleontology 2019Quote: ... Hyaline articular cartilage was dissected and human chondrocytes isolated using 3 mg/mL Collagenase Type II (Worthington Cat # LS004177) in DMEM medium (Sigma-Aldrich ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2021Quote: ... Recipients were immunised with 100µL of 1µg/mL of OVA protein (Worthington Biochemicals) in Freund’s complete adjuvant (CFA ...
-
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Human iPSC-CMs used for patch clamp measurements were dissociated from EHT by collagenase II (200 U/mL; Worthington Biochemical, Lakewood, NJ, USA) for 5 h ...
-
bioRxiv - Neuroscience 2023Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
-
bioRxiv - Developmental Biology 2023Quote: ... in 1:1 DMEM:F12K supplemented with DNase I (Worthington Biochemical), 6.5mg/ml collagenase (Worthington Biochemical) ...
-
bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
-
bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Immunology 2020Quote: ... for a total of 7 days starting two days prior to immunisation with 40μg of ovalbumin (OVA) protein (Worthington) conjugated to 20μm polyethylene carboxylate beads (Polysciences Inc. ...
-
bioRxiv - Neuroscience 2020Quote: ... prepared using 1:1 albumin-ovomucoid solution (10mg/ml of each) (Worthington, Cat# OI ...
-
bioRxiv - Genomics 2023Quote: ... for 1 hour (Patient #1) or 3.7mg/mL collagenase II (Worthington, LS004177) for 90 minutes followed by 6mg/mL dispase (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216), Type 2 (Worthington Biochemical LS004204) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and 1% DNase (Worthington) at 37°C for 6 minutes at which point DMEM (Gibco ...
-
bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Genetics 2020Quote: ... 1 mg ml-1 soybean trypsin inhibitor (#LS003570, all purchased from Worthington Biochemical Corporation), and 1% penicillin/streptomycin in HBSS for 10 min at 37°C with gentle shaking ...
Product cited:
Chromatographically purified as described by Frattali and Steiner, Biochem., 7, 521 (1968). A...Cat# LS003570, 100 mg, $74.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... papain (1 mg/ml, Worthington), ethylenediaminetetraacetic acid (EDTA ...
-
bioRxiv - Immunology 2023Quote: ... 1 mg/ml hyaluronidase (Worthington), 0.1 mg/ml DNase I (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... This solution was then replaced with 1mg/ml collagenase 1 (Worthington Biochemical Corporation CLS-1) in 0.1M PBS and 20mM HEPES for 60-90 min at 37°C followed by 0.05% Trypsin-EDTA (Sigma T9201 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were spun down and supernatant was removed before the addition of 1 ml Dispase-mUE (Dispase II [7 mg ml-1 DMEM Ham’s F12], Neutral Protease, Worthington NPRO2; and 10 μg μL-1 DNase). Cells were aspirated in the solution gently 40 times using a 1 ml pipette tip ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 ml Trypsin (1 g/ml)-DNase (100 mg/ml) solution (Worthington Biochemical, 3703 and 2139) was added to the pellet for 1.5 min at 37°C without disturbing the pellet ...
-
bioRxiv - Microbiology 2023Quote: ... and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals). Plaque forming units (PFU ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml trypsin inhibitor (Worthington), 2 % (v/v ...
-
bioRxiv - Physiology 2019Quote: ... 2.5mg/ml collagenase Type 1 (Worthington) made up in low Ca2+/low Mg2+ HBSS ...
-
bioRxiv - Immunology 2022Quote: ... dispase (0.1 U ml−1; Worthington) and 10% FBS ...
-
bioRxiv - Physiology 2022Quote: ... 2.5mg/ml collagenase Type 1 (Worthington) made up in low Ca2+/low Mg2+ HBSS ...
-
bioRxiv - Microbiology 2021Quote: ... 30 mg ml-1 DNase (Worthington), and 1 mM MgCl2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Deoxyribonuclease 1 (Cat: LS002139, Worthington). Next ...
Product cited:
-
bioRxiv - Neuroscience 2022Quote: ... and 1:1000 DNase (Worthington Biochemical). 0.02% TritonX-100 (v/v ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1 U/mL of dispase (Worthington) and 5uM of Ca2+(Sigma-Aldrich) ...
-
bioRxiv - Developmental Biology 2020Quote: ... with Papain added to 1 mg/ml (1:10 dilution of 10 mg/ml Papain solution; Worthington)) ...
-
bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
-
bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
-
bioRxiv - Genetics 2019Quote: ... + 250 U/ml Collagenase Type 1 (Worthington) + 50 µg/ml DNAse I (Sigma)) ...
-
bioRxiv - Immunology 2019Quote: ... 1 mg/ml Type II collagenase (Worthington), and 50 U/ml Deoxyribonuclease I (Worthington ...