Labshake search
Citations for Worthington Biochemical :
601 - 650 of 767 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... collagenase type 2 (0.8 mg/ml of 250 units per mg; Worthington, NJ, USA), protease XIV (0.12 mg/ml of ≥3.5 units per mg ...
-
bioRxiv - Immunology 2020Quote: ... kidney tissues were minced and digested in 2 mg/ml collagenase type I (Worthington) for 30 min at 37 °C according to a 118 +2 +2 published protocol118 ...
-
bioRxiv - Physiology 2022Quote: ... the perfusate was switched to Tyrode’s solution containing Collagenase (Type 2, 1mg/ml, Worthington) and protease (0.05 mg/ml ...
-
bioRxiv - Pathology 2023Quote: ... The stock collagenase solution was prepared with 25.2 mg of collagenase 2 (Worthington Biochemical) in 48.75 mL of Dulbecco’s Modification of Eagle’s Medium (Corning ...
-
bioRxiv - Cell Biology 2023Quote: The EBs were dissociated by incubation in Collagenase type 2 (0.5 mg/mL, Worthington) in HANKs buffer overnight at room temperature followed by TrypLE for 5 min at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... and transferred to a 15ml tube with 8ml collagenase solution (∼4,000 units in αMEM with 1% anti-mycotic; Type IV, Worthington Biochemical Corp, Lakewood, NJ). The solution containing the tissue fragments was then continuously agitated for 1 hour at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... followed by a 10× dilution using 100 mM Tris-HCl (pH = 8.0) and tryptic digestion under 37°C for 18 hours using 1 µg of trypsin per well (Worthington Biochemical Corp., Lakewood, NJ). Digested peptides were desalted using a panel of lab-made 96-channel filter tips generated by inserting 96 20 µl filter tips into a 96-well cover mat and stacking each filter tip with 5 mg of 10-μm C18 column beads ...
-
bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
-
bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
-
bioRxiv - Cancer Biology 2022Quote: ... The obtained liver tissues were homogenized in 8 mL of RPMI1640 media containing 1.8 mg/ml Collagenase IV (WOR-CLS4-1, Worthington Biochemical Corporation, NJ, USA) and 112.3 µg/ml DNase I (11284932001 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Tissue fragments were incubated in 1.35 mL PBS plus 150 uL 20 mg/mL Collagenase Type I (Worthington CLS-1 dissolved in PBS) and 15 uL 20 mg/ml DNase I (Worthington D dissolved in PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Paleontology 2023Quote: ... the OBM sections were then incubated overnight at 37 degrees Celsius in 1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) with 1% sodium azide dissolved in Dulbecco’s PBS ...
-
bioRxiv - Neuroscience 2023Quote: Retinas from 2 injected mice for each of the different Cy3-labelled siRNAs were pooled and dissociated into single cells using papain for dissociation according to the manufacture’s instructions (Worthington; Cat# 9035-81-1). Each conjugate was done in triplicates with a total of 6 mice per configuration ...
-
bioRxiv - Paleontology 2023Quote: ... The matrix sections were then incubated overnight at 37 degrees Celsius in a solution of ∼1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) dissolved in Dulbecco’s PBS (pH 7.2 ...
-
bioRxiv - Biochemistry 2024Quote: ... we processed approximately 1 g of adipose tissue by mincing and enzymatic dissociation in 0.1% (w/v) collagenase type II (Worthington Biochemical Corporation, Lakewood, NJ) at 37°C for 2 hours with gentle shaking ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 5 minutes at RT in fetal calf serum complemented with DNase I (Worthington), followed by mechanical dissociation ...
-
bioRxiv - Immunology 2021Quote: Perfused lungs were finely minced and digested in 5 mL of a 1.78mg/mL Collagenase IV (Worthington) solution in PBS (with Ca2+ ...
-
bioRxiv - Cancer Biology 2020Quote: ... tissue was enzymatically digested with 5 mL of papain digestion solution (0.94 mg/mL papain (Worthington #LS003119), 0.48 mM EDTA ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Immunology 2020Quote: ... Mice were immunized as indicated in each Figure with 5 μg low-endotoxin OVA (Worthington Biochemical Corporation) together with 50 μg papain (P4762 ...
-
bioRxiv - Cancer Biology 2023Quote: ... minced and digested in 5 mL of Advanced DMEM/F12 containing 10 mg Collagenase Type 3 (Worthington) and 500 U Hyaluronidase (Sigma ...
-
bioRxiv - Physiology 2024Quote: ... bubbled with 95% O2/5% CO2) containing collagenase type I (1.5 mg/ml, Worthington, Lakewood, NJ, USA) and 1% bovine serum albumin ...
-
bioRxiv - Immunology 2023Quote: ... Mice were immunized as indicated in each Figure with 5 µg low-endotoxin OVA (Worthington Biochemical Corporation) mixed with either 50 µg papain (P4762 ...
-
bioRxiv - Immunology 2022Quote: ... biopsies were minced finely with dissection scissors and mixed with 3mL of digestion buffer (0.8mg/mL collagenase type 4 (Worthington) and 0.02mg/mL DNase I (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2019Quote: ... the ventricle was then cut into four pieces and digested in PBS containing 4 mg/ml collagenase II (Worthington) with gentle shaking at 37°C until complete digestion ...
-
bioRxiv - Biochemistry 2020Quote: ... and 1 mL of Ca2+/Mg2+-free HBSS containing 4 mg of collagenase type II and 1.25 mg of Dispase type II (Worthington) was added and incubated at 37°C for 15 minutes ...
-
Pulmonary infection interrupts acute cutaneous wound healing through disruption of chemokine signalsbioRxiv - Immunology 2020Quote: ... The tissue was incubated for 45 minutes at 37° C in DMEM containing type 4 collagenase (Worthington Biochemical Corporation) and DNAse I (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... mechanically cut into small pieces and digested with 0,1 % Collagenase type 2 (265U/mL; Worthington) and DNase I (250U/mL ...
-
bioRxiv - Cell Biology 2020Quote: ... minced lung tissue was transferred to 25 mL pre-warmed collagenase (2 mg/mL, Worthington Biochemical Corporation ...
-
bioRxiv - Developmental Biology 2022Quote: ... were incubated in 2 mg/ml collagenase type II (260 U/mg, Worthington, Lakewood, NJ) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The perfusate was then switched to Tyrode’s solution containing collagen type 2 (Worthington, Columbus, OH) and protease type 14 (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: ... 200 μL of 20 mg/mL of type 2 collagenase in L15 (Cat.No. NC9870009; Worthington) were added and incubated on an orbital rotator at 28°C for 35 min ...
-
bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
-
bioRxiv - Neuroscience 2022Quote: ... Cortices were isolated from postnatal day 0 (P0) CD-1 or cGKII KO mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated dishes for each experiment - 24 wells (200,000 cells ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were removed and the lateral SVZs were dissected in cold DMEM-F12-glutamax 1/50 Glc 45% and subsequently incubated in digestion medium (papain [Worthington], DNase [Worthington], L-cysteine [Sigma-Aldrich]) for 30 min at 37 °C ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and 24 h post-transfection the media was supplemented with 1 μg/mL of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, Lakewood, NJ, USA). The rgH9N1 viruses were propagated on MDCK cells and harvested 72 hours post-inoculations ...
-
bioRxiv - Immunology 2023Quote: ... Virus-infected cells were grown in MEM-BSA-TPCK media[20] that was prepared similar to the MEM media described above but supplemented with 1 µg/mL Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation, Lakewood, NJ) and with Bovine Albumin Fraction V (7.5% solution ...
-
bioRxiv - Cell Biology 2023Quote: ... Half of the samples were treated with a final concentration of 5 μg/mL bacterial collagenase (Worthington Biochemical) for 3 d at 37 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
-
bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... and enzymatically digested at 37°C for 80 min in dispase type II (4.7 mg/ml) plus collagenase type II (4 mg/ml) (Worthington Biochemical), as described previously [62] ...
-
bioRxiv - Neuroscience 2020Quote: ... After adding 0.9 mL pre-warmed HBSS containing 10 mg/mL Collagenase Type 2 (Worthington, UK) samples were incubated for 25 min at 37°C rotating every 5 min and then washed with 2 mL pre-warmed DMEM/F12 supplemented with 10% FBS ...
-
bioRxiv - Immunology 2020Quote: ... and incubated in 2 ml enzymatic solution containing 1.5 mg/ml collagenase type IV (Worthington, LS004188) and 0.5 mg/ml Dnase I (Roche ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... skin was minced and incubated in sterile 0.5% Collagenase Type 2 (Worthington Chemicals, Cat. No. LS004176) overnight at 37 °C in an atmosphere of 5% CO2 and 5% O2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Immunology 2021Quote: ... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
-
bioRxiv - Genetics 2022Quote: ... digested for 30 minutes with 245 units/ml type-2 collagenase (Worthington Biochemical Corp, Lakewood, USA) and 96 μg/ml soybean trypsin inhibitor (Sigma) ...