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Citations for Worthington Biochemical :
151 - 200 of 817 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... as well as on day 1 and 2 after RFA were dissociated using 20 U/ml papain43 (Worthington Biochemical, USA), filtered through a 70 μm filter and resuspended in 3% BSA in PBS ...
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bioRxiv - Microbiology 2019Quote: ... All viral growth was carried out in MEM with 1 μg/ml trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK-treated trypsin; Worthington), 1 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... Surgical samples were minced then digested for 1 hour at 37 °C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cell Biology 2019Quote: ... Kidneys were then cut into small pieces and further dissociated by incubation for 30 min at 37°C in HBSS with 1 mg/ml collagenase (type 2, 255 U/mg, Worthington). To isolate glomeruli ...
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bioRxiv - Physiology 2020Quote: ... minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Ventricular cardiomyocytes isolated from rats or mice at postnatal day 2 or day 1 respectively using the Neonatal Cardiomyocyte isolation System (Worthington) were cultured as previously reported.22 To induce the deletion of Psmc1 ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... mechanically cut into small pieces and digested with 0,1 % Collagenase type 2 (265U/mL; Worthington) and DNase I (250U/mL ...
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bioRxiv - Cell Biology 2020Quote: ... minced lung tissue was transferred to 25 mL pre-warmed collagenase (2 mg/mL, Worthington Biochemical Corporation ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated in 2 mg/ml collagenase type II (260 U/mg, Worthington, Lakewood, NJ) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The perfusate was then switched to Tyrode’s solution containing collagen type 2 (Worthington, Columbus, OH) and protease type 14 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... 200 μL of 20 mg/mL of type 2 collagenase in L15 (Cat.No. NC9870009; Worthington) were added and incubated on an orbital rotator at 28°C for 35 min ...
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bioRxiv - Biophysics 2021Quote: ... The excess tissue was removed from the cerebral cortex and the cortices were minced (0.5 mm) and digested in a solution of Hibernate A (HA)–CaCl2 (Brain Bits LLC) + Papain (2 mg/mL; Worthington, Biochemical Corporation, Lakewood, NJ) for 30 minutes at 37°C with gentle agitation every 5 minutes ...
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bioRxiv - Microbiology 2024Quote: ... cells were cultured in 24-well plates to ∼95% confluency and then infected in triplicate wells for each time point in Opti-MEM™ medium (Thermo Fischer Scientific, Waltham, MA) supplemented with 1 µg/ml L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK, Worthington Biochemicals, Lakewood, NJ). The starting multiplicity of infection (MOI ...
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bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Microbiology 2019Quote: ... tissue supernatants were serially diluted 10-fold in virus infection medium supplemented with 1 µg/mL L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ). The plates were rinsed twice with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... After adding 0.9 mL pre-warmed HBSS containing 10 mg/mL Collagenase Type 2 (Worthington, UK) samples were incubated for 25 min at 37°C rotating every 5 min and then washed with 2 mL pre-warmed DMEM/F12 supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... and incubated in 2 ml enzymatic solution containing 1.5 mg/ml collagenase type IV (Worthington, LS004188) and 0.5 mg/ml Dnase I (Roche ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... skin was minced and incubated in sterile 0.5% Collagenase Type 2 (Worthington Chemicals, Cat. No. LS004176) overnight at 37 °C in an atmosphere of 5% CO2 and 5% O2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Immunology 2021Quote: ... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... digested for 30 minutes with 245 units/ml type-2 collagenase (Worthington Biochemical Corp, Lakewood, USA) and 96 μg/ml soybean trypsin inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Single-cell suspensions were prepared by using Papain containing l-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3 ...
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bioRxiv - Immunology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... Tissue was then incubated with F12K medium containing 2 mg/ml collagenase type IV (Worthington LS004188) at 37°C with pipetting every 5-10 min until most of the glandular cells were released and appeared in solution as clusters ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... and transferred into a digestion buffer consisting of 2 mg/mL collagenase II (LS004176, Worthington Biochemical) and 0.3 mM calcium chloride in CIB ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... The cardiac ventricles were cut into pieces of approximately 1–2 mm3 and dissociated by trituration in 0.05% collagenase type II (Worthington, Lakewood, NJ, USA) 8-10 times for 5 min each ...
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bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... the heart was perfused with the same solutions with 50 μM Ca2+ and collagenase type II (1 mg/mL, CLS-2, Worthington, NJ, USA) for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... and cut into rings (about 1 mm in length) that were subsequently incubated in 2 mg/ml collagenase type II (Worthington Biochemical Corporation) for 2 hr ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples were dissected into small pieces and digested with 1-2 mg/ml (275 U/ml) collagenase type II (Worthington, Columbus, USA) in 30 ml Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: The primary neuronal cells were isolated from the brain tissues of 1∼2-day-old SD pups using the papain dissociation system (Worthington Biochemical Corporation) as instructed by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Cardiomyocytes were dissociated from the ventricles by serial digestions with 0.3 mg/ml Type-2 Collagenase (Worthington) and 0.6 mg/ml pancreatin (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... the enzyme was inactivated by the addition of 2 mL of 0.71 mg/mL ovomucoid (Worthington #LS003087) and 0.06 mg/mL DNaseI (Roche #10104159001 ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003087, 1 gm, $150.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... The isolated IVD tissue was digested in medium containing 0.2% type 2 collagenase (Worthington Biochemical, Lakewood, NK) and 0.3% pronase (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... the bone was cut into small pieces and digested with 2 mg/ml Stemzyme (Worthington, NJ, USA) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Tissues were mechanically cut and digested in complete media containing Collagenase type 2 (265 U/mL; Worthington) and DNase (250 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated in a solution of 2% collagenase type II (Cat # LS004174, Worthington Biochem, Lakewood, USA), prepared in Hanks Balanced Salt Solution (HBSS ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Cell Biology 2021Quote: ... Subcutaneous adipose tissue was minced and digested with collagenase I in PBS (2 mg/ml, Worthington, United States) at 37 °C for 90 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 1.0 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical, NJ, USA), 0.2% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... muscles were chopped in cold PBS and digested using 800 U/ml collagenase type 2 (Worthington, cat# LS004177) in F10 medium (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2022Quote: ... lung tissue was minced and put in digestion buffer (IMDM with 183 U/ml type 2 collagenase (Worthington)) ...
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bioRxiv - Pathology 2022Quote: ... cleaned of all fat and digested for 10 min at 37°C in digestion medium (i.e. isolation medium supplemented with 1.5 mg/mL of collagenase type 2 [#LS004176, Worthington-Biochem] and 0.7 U/mL of elastase type III [#E0127 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2023Quote: ... and placed in a collagenase type 2 solution (500 U/mL in DMEM; Worthington Biochemical, Lakewood, NJ, USA) for 30 minutes in a tissue culture incubator (37°C ...
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bioRxiv - Immunology 2023Quote: ... Lungs were transferred into Miltenyi Gentle MACS tubes containing 2 mL of 2mg/mL collagenase II (Worthington #LS004177) and mechanically ground up by a Gentle MACS machine ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Crude collagenase (Col) type 2 (>125 units per mg; Cat: LS004214) preparations were acquired from Worthington (Lakewood, NJ) and complete Freund’s adjuvant (CFA ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004214, 50 mg, $55.00 Ask