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Citations for Worthington Biochemical :
351 - 400 of 520 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... or in vivo transplantation using an adaptation of a well-established neural cell isolation method (Worthington 9035-81-1).71 As with our undifferentiated hiPSC cultures ...
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bioRxiv - Neuroscience 2024Quote: Neuronal cells were extracted from the brains of 1-day-pold SD pups using a papain dissociation system (Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2024Quote: Kidneys were minced and digested for 20 minutes in 1 mg/mL Collagenase A (Worthington Biochemical, Lakewood, New Jersey). Isolates were pushed through 50 mL conical tube sterile 100 µm cell strainers and washed with cold 35 mL HBSS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: Mouse back skin and wound beds were dissected and digested into single cells using 1:100 collagenase 1A (Worthington) and Liberase TM for macrophage subsets ...
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bioRxiv - Genetics 2022Quote: ... blood was removed by perfusion and the heart was digested with collagenases 2 and 4 (Worthington) and Protease XIV (Sigma) ...
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bioRxiv - Physiology 2021Quote: ... The oocytes were manually defolliculated after treating them with collagenase type 4 (Worthington Biochemical, Lakewood, NJ). Stage V and VI oocytes were selected ...
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bioRxiv - Cell Biology 2020Quote: ... tumors were mechanically dissociated and subjected to enzymatic digestion for 20 min with 200 U ml−1 collagenase IV (Worthington), 5 mM CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as on day 1 and 2 after RFA were dissociated using 20 U/ml papain43 (Worthington Biochemical, USA), filtered through a 70 μm filter and resuspended in 3% BSA in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Frontal and parietal bones were minced and subjected to six sequential enzymatic digestions with a mixture containing 1 mg/mL collagenase type I (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2021Quote: ... Prepontine regions from 5-6 dpf fish were dissected into Evans buffer and dissociated in 1 mg/ml neutral protease (Worthington) in Evans buffer for 30 min at room temperature with gentle shaking ...
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bioRxiv - Neuroscience 2021Quote: ... cerebella from litters of P6 mice were pooled and digested at 37 °C for 20 minutes in 10U ml−1 papain (Worthington), and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by quenching of the enzyme using 1 mg/ml ovomucoid/bovine serum albumin solution with 0.1 mg/ml DNase I (Worthington LK003150). Cells were passed through a 70 μm strainer (PluriSelect 43-10070-70 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Immunology 2020Quote: ... Pieces were further digested for 30 min and then twice for 20 min with a mixture of Collagenase IV (157 Wuensch units ml−1, Worthington), DNAse I (0.2 mg ml−1 dissolved in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... The dissected strips of HTM tissue were digested for 20 min with 1 mg/ml collagenase (Worthington, Lakewood, NJ, USA) and 15 μl/ml human albumin (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... The arteries were then washed three times with Mg2+-PSS and incubated in 1 mg/mL collagenase type II (Worthington) in Mg2+-PSS for 14 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... The dissociation was carried out in dissection buffer containing collagenase B (1-2 mg/ml) and hyaluronidase (1.2-2 mg/ml, Worthington Biochemical) at 37 °C with frequent agitation for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... All viruses were titrated using 1:10 serial dilution in triplicate in LLCMK2 cells in the presence of 2μg/mL TPCK trypsin (Worthington Biomedical) for 72 hours and TCID50/mL was determined by the Reed-Meunch method based on hemagglutination of 0.5% washed chicken red blood cells (Lampire ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were established from autochthonous lung tumors by microdissection and mechanical mincing in digestion buffer (HBSS with 1 M HEPES, 125 Units/mL Collagenase Type IV (Worthington) and 20 μg/mL DNAse (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... The distal lung tissue was separated from the mainstem bronchi and transferred to a Petri dish containing DMEM with 1% HEPES and 100 U/mL DNase I (Worthington). The tissue was then dissociated with a blunt bone marrow aspiration needle ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and the lateral SVZs were dissected in cold DMEM-F12-glutamax 1/50 Glc 45% and subsequently incubated in digestion medium (papain [Worthington] ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% Bovine Serum Albumin (BSA) (MP Biomedical) and 1 µg/ml of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin (Worthington Biomedical) (Weingartl et al. ...
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bioRxiv - Immunology 2022Quote: ... which consisted of HBSS containing Ca2+ and Mg2+ supplemented with 3.4 mg/ml collagenase type 1 (Worthington Biochemical Corporation LS004196) and 100 U/ml hyaluronidase (Sigma-Aldrich H3631) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... Surgical samples were minced then digested for 1 hour at 37 °C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Physiology 2022Quote: ... before perfusion was switched to EGTA-free isolation buffer supplemented with 1 mg/mL collagenase (type II; Worthington Biochemical, USA), 0.06 mg/mL protease XIV ...
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bioRxiv - Physiology 2020Quote: ... minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Immunology 2020Quote: Explanted hearts were cut into pieces and digested for 30 min at 37C with 1 mg/ml of collagenase IV (Worthington) and 0.01% DNase I (Roche) ...
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bioRxiv - Physiology 2020Quote: ... muscles were surgically removed and enzymatically dissociated at 37 °C under mild agitation for ~1 h using 1,000 U/mL of collagenase type IV (Worthington Biochemical). Mechanical dissociation was completed using mild trituration in buffer with no collagenase ...
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bioRxiv - Immunology 2020Quote: ... the aortas were then cut into small pieces (1-2 mm) and incubated for 45 minutes at 37 °C in a type I collagenase (6 mg/ml, Worthington) and elastase (2.5 mg/ml ...
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bioRxiv - Genomics 2020Quote: ... After removal of the epidermal sheets, the dermis was finely minced and enzymatically digested (RPMI basal medium, 1000U/mL collagenase type 1 (Worthington), 40µg/mL DNase I (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Digestion was performed by applying 7 mL 1× HBSS containing 600 U/mL Collagenase II (Worthington Biochemical, Lakewood, NJ, USA) and 60 U/mL DNase I (AppliChem ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were washed 3 times for 3 min in L-15 solution and then placed in L-15 solution containing 7 U ml-1 papain (cat. no. 3126, Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... The minced muscles were centrifuged at 15,000 x g for 1 minute and transferred to 10 ml of digestion buffer (DMEM containing 2.5% HEPES, 1% penicillin/streptomycin, and 400 U/ml collagenase II (Worthington Biochemical)) and placed on an orbital shaker at 100 rpm for 1 hour at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Enzymatic digestion was stopped with 1 mL of 10X hi ovomucoid inhibitor solution and 20 µL 0.4% DNase (Worthington, #LS002007) diluted in 10 mL inhibitor stock solution (ISS) ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... the media was supplemented with 1 µg/ml of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK) treated-trypsin (Worthington Biochemicals, Lakewood, NJ). Wild-type viruses were generated with their respective NP genes (wtNC/19-pdmNP and wtMN/18-trigNP) ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... limb buds from the E12.5 mouse embryo were isolated and digested with 1 mg/ml type II collagenase (Worthington, LS004176) for 1 h at 37 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2022Quote: ... the digested skin was minced and further processed through a 37ºC incubation with 0.2% Collagenase Type 1 (Worthington Biochemical Corporation) in DMEM-F12+ 10% FBS for 1 1/2 hour ...
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bioRxiv - Immunology 2022Quote: ... the digested skin was minced and further processed through a 37ºC incubation with 0.2% Collagenase Type 1 (Worthington Biochemical Corporation) in DMEM-F12+ 10% FBS for 1 1/2 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Mammary glands were minced into 1 mm3 size using a tissue cutting blade and digested with 0.5 mg/mL Collagenase type III (Worthington, LS004182) and 50 U/mL hyaluronidase (Worthington ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... the brain tissues were washed with DM 1∼2 times and incubated with 13mL of DM containing papain (Worthington, LK003176) in 37°C water bath for 20 min ...
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bioRxiv - Immunology 2024Quote: ... Livers were digested at 37°C for approximately 1 h in DMEM medium supplemented with 500 U/ml Collagenase II (Worthington). Erythrocytes were excluded using red blood cell lysis buffer (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: Tumor-transplanted organoids were transferred to a 1.7 mL Eppendorf tube containing 1 mL Papain supplemented with 50 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Microbiology 2024Quote: ... D6/2-rotavirus was propagated in MA104 cells in serum-free DMEM supplemented with 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). Harvested cells were then subjected to three freeze/thaw cycles ...
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bioRxiv - Physiology 2024Quote: ... Aortas were transferred into the 6-well plate and then incubated with 1 mg/ml collagenase II (Worthington Biochemical, LS004174) and 1 mg/ml soybean trypsin inhibitor (Worthington Biochemical ...
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bioRxiv - Cancer Biology 2024Quote: ... Surgical samples were minced then digested for 1 h at 37°C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...