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Citations for Worthington Biochemical :
301 - 344 of 344 citations for Mouse Vasoactive intestinal polypeptide receptor 2 VIPR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Organoid tissue retained on the filter was then back-flushed with 25 ml of cold HBSS and this tissue was further disaggregated in 2 ml Collagenase II (Celase GMP, Worthington Biochemical Corporation, Lakewood, NJ) also containing 200 μg DNAse1 ...
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bioRxiv - Cell Biology 2021Quote: ... Each tissue was cut and minced with scissors and then placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) for 40 min of incubation at 37°C with gentle shaking at 100 rpm ...
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bioRxiv - Biophysics 2021Quote: ... The excess tissue was removed from the cerebral cortex and the cortices were minced (0.5 mm) and digested in a solution of Hibernate A (HA)–CaCl2 (Brain Bits LLC) + Papain (2 mg/mL; Worthington, Biochemical Corporation, Lakewood, NJ) for 30 minutes at 37°C with gentle agitation every 5 minutes ...
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bioRxiv - Microbiology 2019Quote: ... tissue supernatants were serially diluted 10-fold in virus infection medium supplemented with 1 µg/mL L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ). The plates were rinsed twice with PBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 12-to 16-week old) were euthanized and flexor digitorum brevis (FDB) muscles were dissected and incubated with 2 mg/mL collagenase (Worthington Biochemical Co., NJ, USA) in the HEPES-Krebs solution containing 2 mg/mL bovine serum albumin for 2-3 hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM Na-pyruvate) and dissociated them by adding an equal volume of 2 x Papain stock solution (Worthington Biochemical Corporation, Lakewood, USA) at 37°C for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... These chunks were immersed in 5 ml of pre-warmed enzyme solution (2 mg/mL STEMzyme I, 4 µg/mL DNAse I obtained from Worthington Biochemical #LS004107 and #LS002139) in Hanks’ Balanced Salt Solution (HBSS ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004107, 5x50 mg, $495.00 Ask -
bioRxiv - Microbiology 2024Quote: ... The viruses were propagated in MDCK cells in the presence of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ).
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bioRxiv - Developmental Biology 2024Quote: Six corneas from 2 female and 1 male 129S6/SvEvTac mice of each age group (2, 6, and 11-month-old) were dissected and pooled in 600 µL of papain solution (CAT LK003178; Worthington Biochemical Corp., Lakewood, NJ) and incubated for 1.75 hours at 37 °C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Genetics 2020Quote: ... cells were dissociated from transwells with a papain dissociation kit (Worthington Biochemical, Cat# LK003150) and filtered using a Flowmi cell strainer (Bel-Art SP Scienceware ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... by sectioning the optic nerves and were dissociated using the papain kit from Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were isolated from cortical tissue using the papain dissociation kit (Worthington Biochemical Inc.) as previously described [24] [25] and cells were plated at a density of 175,000 cells/cm2 ...
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bioRxiv - Cell Biology 2024Quote: ... the digestion was stopped by a solution of trypsin inhibitors (Leibovitz’s L15 medium, 10% 1 mg/ml trypsin inhibitor [Worthington], and 2% 10 mg/ml DNAse) and cells were washed with L15 medium ...
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bioRxiv - Neuroscience 2021Quote: ... and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington, Cat #:LK003150). The tissue was mechanically triturated and suspended cells were isolated and resuspended in EBSS with albumin-ovomucoid inhibitor solution ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2019Quote: ... single cells were prepared from the bulk tumor by a Papain dissociation kit (Worthington Biochemical) as per the manufacturer’s protocol and cultured using previously reported culturing methods76 ...
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bioRxiv - Cancer Biology 2021Quote: ... E15-P30 Cerebella were dissociated with a Papain dissociation kit following the manufacturer’s instructions (Worthington). Following papain treatment ...
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bioRxiv - Bioengineering 2022Quote: ... Then mechanically minced to 1mm2 pieces and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: Retinas were dissociated into single-cell suspensions using a papain dissociation kit (Worthington, Columbus, OH). Briefly ...
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bioRxiv - Physiology 2019Quote: ... Heart tissue (infarct and surrounding border) was digested in Collagenase II and DNase 1 (600 U/ml Collagenase II; CLS-2 Worthington, 60 U/ml DNAse 1; Ambion, Warrington) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Neuroscience 2020Quote: ... modified to use early post-natal mice and to employ a commercial kit (Worthington Biochemical, LK003150). P1-P4 mice are sacrificed by guillotine method cut just caudal to the ears ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2024Quote: Cerebral organoids were dissociated into a single-cell suspension using Worthington Papain System kit (Worthington, LK003150) according to the manufacturer’s instruction ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Genomics 2022Quote: Primary rat cortical neurons were prepared from E18-19 embryos using a papain dissociation kit (Worthington Biochemical) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: Retinal cells were dissociated into single cells using a Papain dissociation kit (#LK003150, Worthington Biochemicals, Lakewood, NJ, USA), as described by us previously (Ahmed et al. ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2019Quote: ... retinal cells were dissociated into single cell suspensions using a Papain dissociation kit (Worthington Biochemicals, New Jersey, USA). 125 × 103 retinal cells/well were cultured in chamber slides (Beckton-Dickinson ...
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bioRxiv - Neuroscience 2022Quote: ... and the Tissue pieces were dissociated for 30 min at room temperature using the Papain dissociation kit (Worthington) in calcium-free Hibernate A (BrainBits) ...
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bioRxiv - Neuroscience 2022Quote: Individual dorsal forebrain organoids were dissociated using the Worthington Papain Dissociation System kit (Worthington Biochemical, Cat. no. LK003150) following a published protocol (45 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2020Quote: Retinas were rapidly dissected and digested using a papain dissociation kit (catalog no. LK003176; Worthington Biochemical Corporation, Lakewood, NJ) following the manufacturer’s instructions to obtain a homogenous cell suspension ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Biophysics 2021Quote: ... EBSS and Ovomucoid inhibitor were taken from the Papain Dissociation System (PDS Kit, Cat. No.: LK003182, Worthington Biochemical Corporation).
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Neuroscience 2022Quote: Individual brain organoids were dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). A detailed description of the dissociation protocol is available at Protocol Exchange ...
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bioRxiv - Developmental Biology 2024Quote: Organoids were dissociated using a previously described protocol53,78 which is a modification of the Worthington Papain Dissociation Kit manufacturer’s protocol (Worthington, #LK003153). Briefly ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... NBS1- and NBS8-organoids were dissociated to a single cell suspension for 30 minutes using the Papain dissociation kit (Worthington) according to the manufactures’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The hypothalamus and pre-optic area were then dissected from vibratome sections and dissociated into single cells using the Papin Dissociation Kit (Worthington) as previously described24 ...
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bioRxiv - Neuroscience 2023Quote: ... The cortical tissue of each embryo was dissected and then dissociated individually using the Worthington Papain Dissociation Kit (Worthington Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The SVZ was then dissociated into single cell suspension following the manufacturer’s protocol in the papain dissociation kit (Worthington Biochemicals # LK003150)41 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... the proteins were precipitated by trichloroacetic acid (TCA) (PAGE Clean Up Kit, Nacalai Tesque) and then digested with trypsin (TPCK-treated, Worthington Biochemical). The digestion mixture was separated on a nanoflow LC (Easy nLC 1200 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell mixture is centrifuged 6 minutes at 300 x g and after carefully aspirating the supernatant the cell pellet is then resuspended with EBSS plus ovomucoid inhibitor albumin mixture supplemented with DNase (each part of Worthington kit). Gentle trituration with a 10 ml pipette held several centimeters from the tube bottom is sufficient to resuspend ...
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bioRxiv - Developmental Biology 2020Quote: Two organoids per experimental condition were cut into 1 mm2 pieces and enzymatically digested with pa apain digestion kit (Worthington, LK003163) with the addition of 0.5 mg/ml DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Developmental Biology 2023Quote: Two organoids per experimental condition were washed with Ca2+/Mg2+-free DPBS and cut into 1 mm2 pieces and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... 3-4 pooled organoids at 3-4 months of age or three single organoids at 2-month age from both haploinsufficient and corrected lines were transferred to a 60 mm dish containing a papain and DNase solution from the Papain Dissociation Kit (Worthington, #LK003150). Organoids were minced into small bits with razors and incubated for 30 min at 37°C on an orbital shaker ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Pools of cortical organoids from each donor were enzymatically dissociated into a single-cell suspension using the Worthington Papain Dissociation System kit (Worthington Biochemical). Cell suspensions derived from different donors were mixed at equal ratios ...
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bioRxiv - Neuroscience 2023Quote: Whole organoids were dissociated to the single cell level in preparation for single-cell RNA sequencing using the Papain Worthington Kit (Worthington, LK003150). All solutions included in the kit were prepared according to the manufacturer’s instructions ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2020Quote: The organoids cultured from both 24w and 96w plates were dissociated into single cell suspension using a commercially available papain digestion kit (Worthington-biochem, LK003150). Live cells and dead cells from the dissociated organoids were stained with Calcium Green AM (ThermoFisher C3011MP ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... the cerebral cortices were minced with scalpels on an ice-cold culture dish and digested using a papain dissociation kit (Worthington Biochemical, LK003150), according to the manufacturer’s instructions ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask