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Citations for Worthington Biochemical :
251 - 300 of 369 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The tissue was transferred to a pre-warmed 0.125U/mL collagenase solution (Collagenase Type 2, Worthington Biochemical, LS004176) prepared in DMEM (10% FBS ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2024Quote: ... cut into small fragments and dissociated in 2 mL HBSS containing 3 mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... digested for 30 min at 37°C in 2 mg/mL collagenase II (Worthington Biochemical, Lakewood, NJ, USA) in DMEM containing 100 U/mL penicillin and 100 µg/mL streptomycin (GibcoTM ...
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bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... the dissociation buffer was warmed to 34□ and 5 mL was added to a vial of lyophilized papain (Worthington Biochemical Corporation). The solution was diluted 1:2 with additional dissociation buffer prior to sample dissociation.
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bioRxiv - Molecular Biology 2022Quote: ... Primary osteoblasts were isolated from the calvaria of 5-day-old mouse pups using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... This was followed by a wash step and centrifugation at 500 x g for 5 min before removing the dissociation buffer and adding stock collagenase II (4.3 mg/ml) (Worthington, Lakewood, USA) and stock dispase (6.0 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Immunology 2024Quote: ... The digested samples were washed with PBS and were further digested with 0.05% trypsin-EDTA for 5 minutes and 1 unit/ml neutral protease (dispase) (Worthington Biochemical, LS02109) plus 100 ug/ml Dnase (Roche ...
Product cited:
Animal Free, Partially purified. A lyophilized powder.Cat# LS02109, 1 gm, $171.00 Ask -
bioRxiv - Pharmacology and Toxicology 2024Quote: Primary calvarial osteoblasts were isolated from calvaria of 5-day-old neonates using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2024Quote: Cortical assembloids were washed with 1X PBS and dissociated at 37°C 5% CO2 on an orbital shaker with Papain (Worthington #LK003150) for 30 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... and both fragments were separately digested in a petri dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) for 12 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 sutures each from litter 2) were cut into small fragments in HBBS and digested using Collagenase IV (Worthington, USA ...
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bioRxiv - Genetics 2020Quote: ... the cells were resuspended in EBSS containing 50μg/mL of DNase I and 2 mg/mL ovomucoid (Worthington, LK003150). 0.5 volume of EBSS ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2021Quote: ... Lung lobes were minced with forceps and digested with 2 mg/mL Collagenase Type I (Worthington, CLS-1, LS004197), 2 mg/mL Elastase (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2020Quote: Lungs were extracted and placed into dissociation buffer that was composed of 1mg/ml collagenase type 2 (Worthington, #LS004177), 0.25U/ml neutral protease (Worthington ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2020Quote: ... The lysate was then subjected for digestion using L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) treated trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2020Quote: ... The lysate was then subjected for digestion using L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) treated trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 HCT116 cells were used per each immunoprecipitation (IP) reaction and 15 units of micrococcal nuclease (MNase, Worthington) was used to digest the chromatin for the ChIP reaction ...
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bioRxiv - Bioengineering 2024Quote: ... the tissue was first incubated in an enzymatic solution containing 2 mg/mL collagenase II (Worthington Biochemical, Lakewood, NJ) in HyClone Low Glucose Dulbecco’s modified Eagle’s medium (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... The morselized ovary was washed in Ca2+-free buffer to remove yolk from lysed oocytes and then digested at room temperature with gentle shaking for 60-70 minutes in 0.5 mg/ml collagenase type 2 (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation at 37° C for 25 minutes in 3 mg/mL collagenase type 2 (LS004174, Worthington Biochemical) and 2 mg/mL Dispase II (4942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... the lungs were cut in very small pieces and digested in 1.5 ml of Collagenase Type 2 (Worthington, 44N15307B) for one hour at 37°C and 500 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... Fresh schwannomas were minced with sterile Bard-Parker #10 surgical scalpels (Aspen Surgical) and incubated in 0.4% Collagenase Type 2 (Worthington) in pre-oxygenated Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... mouse hearts were removed from 6-8 weeks mice and then digested with 2 mg/ml collagenase (CatNo.LS004196; Worthington). After digestion ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... muscles were chopped in PBS on ice and digested using 800 U/mL collagenase type 2 (Worthington, Cat# LS004177) in F10 medium (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Samples were transferred to tubes with 2 mL inhibitor solution (3.75 mg/mL ovomucoid, 3.75 mg/mL albumin, 100 U/mL DNaseI, Worthington LK003150) and spun for 5 minutes at 200 g ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... fresh lung tissue was minced into 0.1 cm pieces in petri dishes and treated with 2 mg/mL of collagenase D (Worthington) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2024Quote: We extracted cells from the central body of freshly dissected menisci from 3-week-old WT and Col5a1+/− mice by 0.2% collagenase (CLS-2, Worthington) digestion for 5 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... a total of ∼50 brains for each group was immediately transferred to an Eppendorf tube (1.5 mL) containing 200 µL AHS with Pronase (1 mg/mL; P5147, SigmaAldrich) and Dispase (1 mg/mL; LS02104, Worthington Biochemical Corporation). After tissue digestion for 30 min at 25°C ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... washed twice in HBSS and incubated during 30 min at 37°C in pre-warmed, oxygenated (95% O2, 5% CO2) Papain/DNase I (Worthington Biochemical Corporation) on a gyratory shaker (100 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Genomics 2022Quote: ... Nuclei were collected by centrifugation and DNase I-treated for 30 min at 37°C (Worthington, 30U per 5 x 106 cells). After DNase I digestion ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated statically at 37°C with 5% CO2 followed by plating on TSA plates supplemented with 100 µl of catalase (38,000 U/ml; Worthington Biochemical Corporation, NJ) and the desired antibiotic (250 µg/ml kanamycin or 200 µg/ml streptomycin) ...
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bioRxiv - Molecular Biology 2022Quote: Five 10um cryostat slices of patient polyps were digested for 5 min at 37°C with a titration of MNase: 20 units/mL and 200 unit/mL of MNase (Worthington Biochemical Corp.) in MNase cleavage buffer (4 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as on day 1 and 2 after RFA were dissociated using 20 U/ml papain43 (Worthington Biochemical, USA), filtered through a 70 μm filter and resuspended in 3% BSA in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... The dissected hypothalamuses were further divided into 2-4 pieces before cell dissociation for single cell isolation using Papain Dissociation System (cat no. LK003150, Worthington Biochemical Corporation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Immunology 2021Quote: ... tissue disaggregation was performed by finely chopping liver samples (~1-2 g) in 10 ml digestion solution containing 1 mg/ml Collagenase IV (Worthington) and 20 μg/ml DNAse1 (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... coli was removed by mixing TNKS1 construct with 2 µM snake venom phosphodiesterase I from Crotalus adamanteus (Worthington Biochemical Corporation).
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bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
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bioRxiv - Immunology 2022Quote: Lung tissue was minced and digested in a RPMI 1640 solution with Type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Surgical samples were minced then digested for 1 hour at 37 °C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The tumor-bearing left lungs were mechanically dissociated using a razor blade and incubated for 30 minutes at 37°C with 3.2mg/mL Collagenase Type 2 (Worthington, 43C14117B), 0.75 mg/ml Elastase (Worthington ...
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bioRxiv - Physiology 2020Quote: ... minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Immunology 2020Quote: ... the aortas were then cut into small pieces (1-2 mm) and incubated for 45 minutes at 37 °C in a type I collagenase (6 mg/ml, Worthington) and elastase (2.5 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...