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Citations for Worthington Biochemical :
201 - 250 of 329 citations for N 2 2 2 2 Aminoethyl amino ethyl amino ethyl amino ethyl 9 12 15 octadecatrienamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Biochemistry 2022Quote: ... Each 25 μL piece of vitreous was placed in an 8 mm diameter coverslip-bottom chamber with 200 μL of either 2 mg/mL bacterial collagenase type II (Worthington) or phosphate-buffered saline ...
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bioRxiv - Neuroscience 2022Quote: ... and the sensory neurons were dissociated by mechanical agitation after incubation for 2 h with collagenase type III (CLS3, Worthington) at 37°C ...
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bioRxiv - Genomics 2024Quote: ... we replaced the medium with 2 mL of digestive enzyme mixture I (3 mg/mL collagenase I (Worthington, cat. LS004196), 3 mg/mL collagenase II (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...
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bioRxiv - Immunology 2023Quote: ... A 2 cm2 patch of skin was minced and incubated in 3 mg/mL collagenase Type IV (Worthington Biochemical Corporation) and 2 mg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro degradation of lignin composites was determined by submerging lignin composites in the solution of collagenase type II (0.5 U/mL, cat# CLS-2, Worthington Biochemical) with 1 mM CaCl2 in serum-free culture medium ...
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bioRxiv - Immunology 2023Quote: ... whole lung was minced with a sharp razor blade and digested with collagenase type IV 2 mg/ml (Worthington, LS004189) and deoxyribonuclease II 4 μg/ml (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the brain tissues were washed with DM 1∼2 times and incubated with 13mL of DM containing papain (Worthington, LK003176) in 37°C water bath for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... D6/2-rotavirus was propagated in MA104 cells in serum-free DMEM supplemented with 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). Harvested cells were then subjected to three freeze/thaw cycles ...
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bioRxiv - Neuroscience 2024Quote: ... samples were pre-cut with small scissors and diluted and digested in 2:3 stock solution of CollagenaseI (10 U/ml, Worthington), Collagenase IV (400 U/ml ...
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bioRxiv - Immunology 2024Quote: ... The cardiac tissues were minced and subjected to repetitive rounds of enzymatic digestion with collagenase type 2 (Worthington Biochemical Corp.) until complete dissociation ...
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bioRxiv - Immunology 2024Quote: Lung tissue was minced and digested in an RPMI 1640 solution with type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Surgical samples were minced then digested for 1 h at 37°C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were minced and digested using a freshly prepared enzymatic mix containing Collagenase type 2 (2.5 mg/ml; Worthington, LS004174), DNase I (125 U/ml ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C in serum-free cell culture medium (IMDM) supplemented with 1 µg/ml L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) trypsin (Worthington-Biochemical). Cell supernatants were harvested at indicated time points post-infection ...
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bioRxiv - Neuroscience 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... A 5 to 7-day-old protonemal tissue culture was scraped from solid plates and added to a 466 mM mannitol solution with 2% Driselase and Cellulase (21 unit/mL, Worthington CEL). The mixture was gently agitated for 1 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.), 1 mM EDTA (PanReac AppliCham ...
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bioRxiv - Physiology 2022Quote: ... after intracardiac perfusion of Phosphate Buffer Saline followed by a solution of HBSS containing type II collagenase at 2 mg/ml (Worthington, USA). Specific diets described below were introduced the day of the first dimerizer injection and maintained until sacrifice.
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bioRxiv - Cell Biology 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Unlabeled and Alexa Fluor 488 (AF488)-labeled T3DCD or T1L virions (2×1012 particles/ml) were digested with 200 μg/ml Nα-p-tosyl-L-lysine chloromethyl ketone-treated chymotrypsin (Worthington Biochemical) in a total volume of 100 μl for 20 min at 32°C [34] ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2; Worthington Biochemical) in 5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... with 2 mL pre-warmed digestion enzyme in ACSF solution (Papain, 25 unit/mL and DNase I, 55 unit/mL, Worthington Biochemical). For spinal dorsal horn neuronal atlas construction ...
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bioRxiv - Cell Biology 2024Quote: ... whole hearts from 28 wk-old ♂ WT and R14Δ/+ mice were cannulated and perfused with modified Krebs buffer containing 2 mg/ml collagenase (Worthington) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Biochemistry 2024Quote: ... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
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bioRxiv - Genetics 2023Quote: ... and perfused with a type 2 collagenase digestion buffer (2.4 mg/ml concentration) at a flow rate of 4 ml/min (Worthington Cat# LS004176). The large blood vessels and the atria were excised from the ventricles and minced in a stop buffer containing 10% calf serum ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Cancer Biology 2024Quote: ... minced into 1-2 mm3 pieces and then placed into a digestion solution containing 0.5 mg mL-1 collagenase D (Worthington Biochemical Corporation), 0.01 mg mL-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: Day 25 flushed bovine chimeric embryos were cut into small pieces and treated with 2 mg/ml papain (Worthington Biochemical, LS003119), 1 mg/ml DNase I (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... The cardiac ventricles were cut into pieces of approximately 1–2 mm3 and dissociated by trituration in 0.05% collagenase type II (Worthington, Lakewood, NJ, USA) 8-10 times for 5 min each ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
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bioRxiv - Genomics 2020Quote: ... Mouse adult cardiocytes were obtained by digesting adult mouse hearts by retrograde perfusion of a solution of collagenase type 2 (Worthington, Lakewood, NJ) in calcium-free solutions ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Bioengineering 2021Quote: ... Muscles were minced to create a homogenous cell slurry and then incubated for 2 h at 37C in 0.2% collagenase (Worthington Biochemicals, Lakewood, NJ) in DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... ovarian cortex samples were digested in DMEM containing 25 mM HEPES and 2 mg/mL collagenase type III (Worthington Biochemical Corporation, LS004183) for 2 hours at 37°C with occasional swirling ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... The hippocampal neurons were then dissociated via incubation at 37°C for 30 minutes in 2% papain solution (Worthington; catalog no. LS003127). Following incubation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Physiology 2020Quote: ... the heart was perfused with the same solutions with 50 μM Ca2+ and collagenase type II (1 mg/mL, CLS-2, Worthington, NJ, USA) for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Molecular Biology 2023Quote: ... and cut into rings (about 1 mm in length) that were subsequently incubated in 2 mg/ml collagenase type II (Worthington Biochemical Corporation) for 2 hr ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples were dissected into small pieces and digested with 1-2 mg/ml (275 U/ml) collagenase type II (Worthington, Columbus, USA) in 30 ml Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
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bioRxiv - Neuroscience 2023Quote: The primary neuronal cells were isolated from the brain tissues of 1∼2-day-old SD pups using the papain dissociation system (Worthington Biochemical Corporation) as instructed by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... The bulb of each DRG was cut into 3mm sections and placed in 5mL of pre-warmed digestion enzyme containing 2 mg/mL of Stemxyme I (Worthington Biochemical, LS004106), 10ng/mL of recombinant human β-NGF (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: ... cells were cultured in 24-well plates to ∼95% confluency and then infected in triplicate wells for each time point in Opti-MEM™ medium (Thermo Fischer Scientific, Waltham, MA) supplemented with 1 µg/ml L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK, Worthington Biochemicals, Lakewood, NJ). The starting multiplicity of infection (MOI ...