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Citations for Worthington Biochemical :
201 - 250 of 365 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... The ventricles were then minced and incubated with 2mg/mL collagenase type 2 (Worthington Biochemical, LS004176) in Hank’s balanced salt solution (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested in 6 mL of 2 mg/mL type IV collagenase (Worthington Biochemical; cat. #LS004189) in PBS with 1% FBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... was minced and incubated in 2 mg/ml collagenase type II digestion solution (Worthington Biochemical Corporation) for 2 hr ...
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bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Neuroscience 2021Quote: ... pH adjusted to 7.6] and mechanically dissociated to single cells after adding ovomucoid (Worthington, 0.7 mg per 5 mice) and DNAse (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in Dispase II (5 mg/mL; StemCell 7913) and DNase I (100 μg/mL; Worthington Biochemical LS002006) for 5 minutes ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... lungs were perfused with 5 ml PBS and then digested with 4 U/ml elastase (Worthington Biochemical Corporation, NJ) and 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma, cat # 4942078001], 0.5 mg/ml DNAse I [Worthington Biochemical ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... The nuclei suspension was preincubated at 37°C for 5 min and followed by micrococcal nuclease (Worthington, cat# LS004798) digestion was carried out by following the manufacturer’s protocol ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004798, 45 ku, $211.00 Ask -
bioRxiv - Microbiology 2022Quote: ... lung tissue was minced in 5 ml of 1 x PBS containing 3 mg/ml collagenase type IV (Worthington). Samples were incubated at 37°C for 45 min and washed with 1 x PBS three times ...
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bioRxiv - Physiology 2023Quote: ... The muscle layer was cut into pieces and incubated in 5 ml of digestive solution containing 1.5 mg collagenase (Worthington), 1 mg trypsin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were centrifuged for 5 minutes and the pellet was resuspended with DNAase/ovomucoid inhibitor according to manufacturer’s protocol (Worthington). Cells were then centrifuged and resuspended on ice in Leibovit’z L-15 medium supplemented with 0.1% of bovine serum albumin ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryonic single cell suspensions from were obtained by performing eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (0.7mg/ml Worthington) at 37°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... The remaining tissue was then incubated in 5 mL of digest solution [200 U/mL Collagenase IV (Worthington, LS004189) and 400 U/mL DNase I (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2024Quote: ... cut into small ∼1mm3 pieces and enzymatically digested in DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... Cardiomyocytes were dissociated from the ventricles by serial digestions with 0.3 mg/ml Type-2 Collagenase (Worthington) and 0.6 mg/ml pancreatin (Sigma ...
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bioRxiv - Immunology 2022Quote: ... in DMEM containing 0.0002% L-1-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical) with antibiotics ...
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bioRxiv - Cancer Biology 2020Quote: ... the enzyme was inactivated by the addition of 2 mL of 0.71 mg/mL ovomucoid (Worthington #LS003087) and 0.06 mg/mL DNaseI (Roche #10104159001 ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003087, 1 gm, $150.00 Ask -
bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical] ...
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bioRxiv - Immunology 2022Quote: ... and digested in 6 ml of 2-mg/mL type II collagenase (Sigma # C6885 or Worthington #LS004177) for 30 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... the bone was cut into small pieces and digested with 2 mg/ml Stemzyme (Worthington, NJ, USA) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Tissues were mechanically cut and digested in complete media containing Collagenase type 2 (265 U/mL; Worthington) and DNase (250 U/mL ...
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bioRxiv - Immunology 2023Quote: ... and digested in 6 ml of 2 mg/mL type IV collagenase (Worthington Biomedical, Lakewood, NJ, #LS004186) for 30 min at 37°C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... The isolated IVD tissue was digested in medium containing 0.2% type 2 collagenase (Worthington Biochemical, Lakewood, NK) and 0.3% pronase (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Prepontine regions from 5-6 dpf fish were dissected into Evans buffer and dissociated in 1 mg/ml neutral protease (Worthington) in Evans buffer for 30 min at room temperature with gentle shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM MgCl2) and LC-MS/MS was performed by on-bead digestion with trypsin (TPCK-treated, Worthington Biochemical).
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Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous TransplantationbioRxiv - Cell Biology 2020Quote: ... (Fat Processing Video) Phosphate-buffered saline (PBS; 100 mL) was mixed with 75 mg (±5 mg) of type A collagenase (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Physiology 2023Quote: ... Homogenate was digested with liver digestion buffer (5 ml HBSS w/o Ca2+/Mg2+, 200U/ml Collagenase IV (Worthington, USA) (37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The material was then transferred to a 5 mL microcentrifuge tube containing 2.5 mL Papain supplemented with 125 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue was enzymatically and mechanically digested for 5-10 minutes at 37°C in HBSS-papain-based lysis buffer (Worthington) containing DNAse (0.01% ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary tumor and metastasized lung tissues were digested in 5 mL of enzyme buffer containing 0.2 mg/mL collagenase type-II (Worthington, LS004174), 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Subcutaneous adipose tissue was minced and digested with collagenase I in PBS (2 mg/ml, Worthington, United States) at 37 °C for 90 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 1.0 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical, NJ, USA), 0.2% bovine serum albumin (BSA ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... muscles were chopped in cold PBS and digested using 800 U/ml collagenase type 2 (Worthington, cat# LS004177) in F10 medium (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2022Quote: ... lung tissue was minced and put in digestion buffer (IMDM with 183 U/ml type 2 collagenase (Worthington)) ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated ganglia were cleaned from excess nerve tissue and incubated with type 1 collagenase (2 mg/ml; Worthington) and dispase (5 mg/ml ...
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bioRxiv - Pathology 2022Quote: ... cleaned of all fat and digested for 10 min at 37°C in digestion medium (i.e. isolation medium supplemented with 1.5 mg/mL of collagenase type 2 [#LS004176, Worthington-Biochem] and 0.7 U/mL of elastase type III [#E0127 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2023Quote: ... and placed in a collagenase type 2 solution (500 U/mL in DMEM; Worthington Biochemical, Lakewood, NJ, USA) for 30 minutes in a tissue culture incubator (37°C ...
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bioRxiv - Immunology 2023Quote: ... Lungs were transferred into Miltenyi Gentle MACS tubes containing 2 mL of 2mg/mL collagenase II (Worthington #LS004177) and mechanically ground up by a Gentle MACS machine ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Crude collagenase (Col) type 2 (>125 units per mg; Cat: LS004214) preparations were acquired from Worthington (Lakewood, NJ) and complete Freund’s adjuvant (CFA ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004214, 50 mg, $55.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... The tissue was transferred to a pre-warmed 0.125U/mL collagenase solution (Collagenase Type 2, Worthington Biochemical, LS004176) prepared in DMEM (10% FBS ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2024Quote: ... cut into small fragments and dissociated in 2 mL HBSS containing 3 mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... digested for 30 min at 37°C in 2 mg/mL collagenase II (Worthington Biochemical, Lakewood, NJ, USA) in DMEM containing 100 U/mL penicillin and 100 µg/mL streptomycin (GibcoTM ...
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bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...