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Citations for Worthington Biochemical :
2351 - 2400 of 2781 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tissue was then titurated in a digestion media containing 10 U/ml elastase (#LS002280, Worthington Biochemical, Lakewood, NJ, USA) and incubated for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 U/mL Collagenase type 3 (Worthington, LS004183), 20 µg/mL Liberase Blendzyme 2 (Roche ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... 20 U/ml papain (Worthington, LS003126)) for 25-40 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and incubated in 2 ml enzymatic solution containing 1.5 mg/ml collagenase type IV (Worthington, LS004188) and 0.5 mg/ml Dnase I (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed in RPMI media containing 20 U/mL papain (Worthington Biochemical LS003119) and 0.005% DNAase I ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were digested in papain 20U/ml (Worthington, CAT# LS003126), DNAseI 80U/ml (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... and excess DNA is removed using DNAse (Worthington LS002139). For FACS experiments on mammary organoids ...
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bioRxiv - Immunology 2020Quote: ... inflated with a 1.5 ml mixture of 1 ml low melting agarose (3% w/v) and 500 μl 4U/ml dispase (Worthington Biochem, LS02104), and incubated for 1 h at room temperature (RT) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Cancer Biology 2020Quote: ... Chopped tissue was digested in 100 U/mL Collagenase Type 1 (Worthington Biochemical Corporation, Cat# LS004196), 500 U/mL Collagenase Type 4 (Worthington Biochemical Corporation ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... enzymatic digestion was achieved by 3 min of perfusion with calcium-free digestion buffer (400 units/mL of collagenase II in perfusion buffer; Worthington LS004177) followed by 12 min of perfusion with digestion buffer containing 50 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2020Quote: ... and then treated with DNaseI (Worthington Biochemical, cat. no. LS002139). The cells were filtered through a 100 µm strainer ...
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bioRxiv - Developmental Biology 2020Quote: ... The hearts from all embryos were then sorted into two tubes based on fluoresce signal and digested with 300μl 500 U/ml collagenase IV (Worthington #LS004186), 1.2 U/ml dispase (Worthington #LS02100) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.2 U/ml dispase (Worthington #LS02100), 32 U/ml DNase I (Worthington #LS002007) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Genomics 2020Quote: ... Chromatin was fragmented into ∼90% mononucleosome and ∼10% dinucleosome with Micrococcal Nuclease (MNase) (Worthington Biochem #LS004798). We recommend titrating the MNase digesting condition for each sample ...
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bioRxiv - Developmental Biology 2020Quote: ... and collagenase (1 mg/mL, Worthington, no. LS004214) was used to isolate cochlear epithelia as previously described (90) ...
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bioRxiv - Immunology 2020Quote: ... tumors were harvested and dissociated in Collagenase IV (Worthington Biochemical, cat. LS004189) Buffer (1x HEPES buffer ...
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bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... Ventricles of three hearts were micro-dissected and pooled into a 600 μl mix consisting of 1.2 U/ml dispase (Worthington #LS02100), 500 U/ml collagenase IV (Worthington #LS004186) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... Neurons were then treated with Papain (Worthington #LS003120) and DNAse (VWR #V0335)-supplemented Hibernate A for 18min at 37°C and washed twice with plating medium (plating medium ...
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bioRxiv - Developmental Biology 2020Quote: ... 32 U/ml DNase I (Worthington #LS002007), and sterile DPBS with Mg2+ and Ca2+ ...
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bioRxiv - Systems Biology 2020Quote: ... and DNase I (125 Kunitz ml-1, Worthington, #LS002058) for 25-30 min at 10°C with frequent agitation at every 5min ...
Product cited:
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for...Cat# LS002058, 11 mg, $110.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... the cells were washed with PBS 48 hours after viral transduction and digested for 30 minutes at 37°C with a papain (Worthington, LS003119) solution (2mg/ml KnockOutTM DMEM without calcium ...
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bioRxiv - Neuroscience 2020Quote: ... 0.4 ml Collagenase/Dispase (20mg/ml, cat.n.LS004106, Worthington)) for 1 h at 37°C with triturating every 15 min through a 1 ml tip cut to a ∼5 mm opening ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol and 0.075% NP-40) before being exposed to MNase (Worthington, LS004798) digestion titration to generate the appropriate size of chromatin fragments as follow ...
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bioRxiv - Genomics 2020Quote: ... Chromatin digestion was carried out at 30°C for 12 minutes with different concentrations of micrococcal nuclease (MNase, Worthington Biochemicals; cat# LS004798). The digestion was stopped by adding EDTA to final concentration of 10 mM ...
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bioRxiv - Genetics 2020Quote: ... followed by addition of 4 U MNase (Worthington Biochemical LS004798; Lakewood, NJ) per 1 × 107 cells ...
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bioRxiv - Genomics 2020Quote: ... and 100 µg/ml elastase (Worthington LS006365) in RPMI (Gibco 72400120 ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining cortices were diced and digested with Papain (Worthington #LS003126; 330 units) for 1 hr and 15 min in 37C 10% CO2 cell culture incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.1 mg/mL DNase I (Worthington, #LS002006). Cell pellets were incubated in ACK lysis buffer for 5 minutes and rinsed twice in FACS buffer (BD Pharmingen ...
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bioRxiv - Developmental Biology 2020Quote: ... 500 U/ml collagenase IV (Worthington #LS004186), 32 U/ml DNase I (Worthington #LS002007) ...
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bioRxiv - Cell Biology 2020Quote: Tissues were digested enzymatically for 30 minutes at 37°C with papain (Worthington, LS003126) 20 units/mL in Ames ...
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bioRxiv - Cell Biology 2020Quote: ... 0.18 mg/mL of Type IV Collagenase (Worthington Biochem Catalog: LS004188), BSA and collagenase were added immediately before use ...
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bioRxiv - Immunology 2020Quote: Histones (Worthington cat#LS002546/lot#33P14617) were administered at 50 mg/kg via retro-obital injection.
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bioRxiv - Cancer Biology 2020Quote: ... followed by digestion with trypsin-TPCK (Worthington, LS003744, 10 mg/mL final) overnight at room temperature ...
Product cited:
A chromatographically purified, diafiltered, lyophilized powder that has been treated with...Cat# LS003744, 1 gm, $675.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissers and incubated in 1 ml PBS supplemented with collagenase (4mg/ml Worthington Biochemical, LS004176), dispase (2mg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and collagenase type I (3 mg/mL, Cat# LS004194, Worthington, Lakewood, NJ) and incubated for 50 minutes at 37°C under gentle agitation ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2020Quote: ... 500 U/mL Collagenase Type 4 (Worthington Biochemical Corporation, Cat# LS004188), and 200 µg/mL DNase I (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... and it was then perfused with type II collagenase (Worthington #LS004176). Calcium was reintroduced to the subsequent single cell suspension and cells were plated on laminin (Corning 354232 ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected retinas were enzymatically digested with 9 units/mL of papain (#LS003126; Worthington, Lakewood, NJ) for 30 mins at 34°C followed by trituration ...
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bioRxiv - Developmental Biology 2020Quote: ... 250μl 0.5mg/ml DNAse (Worthington Biochemical ...
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bioRxiv - Developmental Biology 2020Quote: ... dissociated to single cells with trypsin and Dnase (Worthington Biochemical 3703 and 2139), and separated on a 35%/60% percoll gradient resulting in two layers of cells ...
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bioRxiv - Physiology 2020Quote: ... HBSS containing 0.04% Collagenase Type 2 (Worthington), 50 mM HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid) ...
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bioRxiv - Plant Biology 2020Quote: ... The unfertilized ovules of each cell marker line were treated with enzyme solution (1 % cellulase [Worthington, USA] ...
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bioRxiv - Developmental Biology 2020Quote: ... or Rbx2cKO-Emx1 embryos and neurons were dissociated using papain (Worthington Papain Dissociation Systems cat# LK003150) ...
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bioRxiv - Cancer Biology 2020Quote: ... Micro-dissected regions were dissociated by incubating with papain (20 units/ml) DNase (0.005%) for 30min at 37°C (Worthington). Samples were triturated in HEPES buffered EBSS to fully dissociate the tissue and centrifuged (3 min ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5 U/μL of MNase (Worthington Biochemical Corporation) and CaCl2 were added to 7.4 mM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The perfusate was then switched to Tyrode’s solution containing collagen type 2 (Worthington, Columbus, OH) and protease type 14 (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... and 1.5 mg trypsin (Worthington). Digestion was stopped with 1 ml culture medium containing 80% DMEM (Lonza) ...