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Citations for Worthington Biochemical :
51 - 100 of 432 citations for 4 2 CHLORO 3 PYRIDYL 4 OXOBUTYRONITRILE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The digestion mix contained 0.8 mg/mL Collagenase Type 4 (Worthington; LS004186) and 0.04 mg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 2018.39 Cortices of 4 mice were dissociated using papain (Worthington PAP2 3176) and plated on 75cm2 tissue culture flasks coated with poly-D-lysine (Sigma P6407) ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested with 1 mg/mL collagenase type 4 (Worthington, Cat# LS004188) in RPMI 1640 with 50 µg/mL DNase I at 37°C for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated with 10 mg/ml collagenase IV (Worthington Biochemicals, CLS-4) in Hanks Balanced Salt Solution (HBSS ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were dissociated using 1 mg/mL collagenase type 4 (Worthington, Cat# LS004188) in RPMI 1640 supplemented with 50 µg/mL DNase I (MP Biological ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by enzymatic digestion with dissociation solution: 2.5 mg/mL collagenase 4 (Worthington) and 200 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 600 μL of 10 mg/mL collagenase type 4 (Worthington Biochemical cat# LS004186) prepared in HBSS-free media (Thermo Fisher cat#14175-095) ...
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bioRxiv - Immunology 2022Quote: Lungs were digested for 40 min in 1 mg/ml collagenase type 4 (Worthington) 5% FBS in DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... 4 cultures were pooled and dissociated using the Papain Dissociation System (Worthington Biochemicals, LK003150) according to manufacturer’s instructions ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... followed by perfusion with enzymatic digestion solution containing 0.3 mg/mL Collagenase Type 4 (Worthington), 12.5 μg/mL protease ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then suspended in a solution of 1mg/ml Collagenase Type 4 (Worthington, LS0004186) and incubated at 37°C for 30min ...
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bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... cortices were isolated from postnatal day 2-3 mouse pups and dissociated with Papain (Worthington) in DMEM (Gibco ...
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bioRxiv - Physiology 2021Quote: ... The oocytes were manually defolliculated after treating them with collagenase type 4 (Worthington Biochemical, Lakewood, NJ). Stage V and VI oocytes were selected ...
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bioRxiv - Cell Biology 2021Quote: ... Minced tissue underwent 3-5 serial digestions in collagenase type 2 (Worthington Biochemical Corp, Lakewood, NJ) in sterile PBS with 20 mM glucose ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Immunology 2024Quote: ... Tumors were finely minced and digested in RPMI-1640 with 4 mg ml-1 collagenase IV (Worthington) and 0.1 mg ml-1 DNaseI (Sigma ...
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bioRxiv - Immunology 2024Quote: ... for RNA extraction or into a digestion buffer containing 1.6 mg/ml collagenase type 4 (Worthington LS004189) and 100 µg/ml DNase I (Roche 11284932001 ...
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bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical] ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Right lung lobes were minced and digested with 5 mL digestion buffer: Collagenase 4 (200U/mL, Worthington Biochemicals), DNase I (100 ug/mL ...
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bioRxiv - Immunology 2021Quote: ... Cells were then DNAse treated with 4 ml dissagregation media (DMEM, 10% FBS, 100 μg/ml DNAse (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and they were further digested for 30 min in 4 mg/ml collagenase II (Worthington, cat. No. LS004176) and 4.66 mg/ml dispase II (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with 3% collagenase type 3 (Worthington), 3 U/ml dispase (Worthington ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2024Quote: ... cut into small fragments and dissociated in 2 mL HBSS containing 3 mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I for 30 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... biopsies were minced finely with dissection scissors and mixed with 3mL of digestion buffer (0.8mg/mL collagenase type 4 (Worthington) and 0.02mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mL of Ca2+/Mg2+-free HBSS containing 4 mg of collagenase type II and 1.25 mg of Dispase type II (Worthington) was added and incubated at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... for 4 minutes at a flow rate of 5ml/min followed by 0.8mg/ml Type 1 Collagenase (Worthington, UK) in Dulbecco Minimum Essential Medium (Gibco # 11965-092 ...
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Pulmonary infection interrupts acute cutaneous wound healing through disruption of chemokine signalsbioRxiv - Immunology 2020Quote: ... The tissue was incubated for 45 minutes at 37° C in DMEM containing type 4 collagenase (Worthington Biochemical Corporation) and DNAse I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... lungs were perfused with 5 ml PBS and then digested with 4 U/ml elastase (Worthington Biochemical Corporation, NJ) and 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... and skin was cut into small pieces and mixed with digestion buffer containing 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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bioRxiv - Neuroscience 2024Quote: ... Ganglia were placed into a digestion buffer containing 1 mg/ml (325 U/ml) collagenase type 4 (Worthington LS004189), 2 mg/ml (1.8 U/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 sutures each from litter 2) were cut into small fragments in HBBS and digested using Collagenase IV (Worthington, USA ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation at 37° C for 25 minutes in 3 mg/mL collagenase type 2 (LS004174, Worthington Biochemical) and 2 mg/mL Dispase II (4942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Bioengineering 2024Quote: We extracted cells from the central body of freshly dissected menisci from 3-week-old WT and Col5a1+/− mice by 0.2% collagenase (CLS-2, Worthington) digestion for 5 h at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and enzymatically digested at 37°C for 80 min in dispase type II (4.7 mg/ml) plus collagenase type II (4 mg/ml) (Worthington Biochemical), as described previously [62] ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was then digested for 2 hr at 37 °C in 1/10 volume of 1 M ammonium bicarbonate with snake venom phosphodiesterase (1.2×10−4 U/μg RNA) and bacterial alkaline phosphatase (0.1U/ μg RNA) (Worthington Biochemical corporation). Analysis was performed on a TSQ Quantiva Triple Quadrupole (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
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bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Immunology 2023Quote: ... A 2 cm2 patch of skin was minced and incubated in 3 mg/mL collagenase Type IV (Worthington Biochemical Corporation) and 2 mg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... we replaced the medium with 2 mL of digestive enzyme mixture I (3 mg/mL collagenase I (Worthington, cat. LS004196), 3 mg/mL collagenase II (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask