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Citations for Qiagen :
351 - 400 of 10000+ citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... A customized RT2 Profiler PCR array for rat angiogenesis markers (PARN 042Z, 330231, Qiagen) containing SYBR Green RT PCR assays for 84 genes of interest ...
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bioRxiv - Cell Biology 2022Quote: Alox12b and Tgm1 shRNA knockdown in rat epidermal keratinocytes using SureSilencing shRNA plasmids (Qiagen) has been previously described (O’Shaughnessy et al ...
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bioRxiv - Genomics 2021Quote: Genomic DNA from MCF7 cells was isolated by proteinase K digestion at 65°C for 30 minutes followed by purification using the QIAamp circulating nucleic acid kit (Qiagen, Venlo, The Netherlands). Genomic DNA from frozen tissue sections of colorectal liver metastases was isolated using the NucleoSpin Tissue kit according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... was digested overnight at 56°C in 20 ul proteinase K and 180 ul Buffer ATL from the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, California, USA). DNA was extracted from the digest with the QIAamp PowerFecal DNA Kit (Qiagen ...
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bioRxiv - Physiology 2019Quote: ... to remove the cell debris and were stored at −80°C for no longer than 4 weeks until isolation of cf DNA with QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany) and measurement of plasma concentrations of cf n-DNA and cf m-DNA with real time quantitative PCR as described previously3 ...
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bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
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bioRxiv - Microbiology 2021Quote: ... Filters were flash-frozen in liquid nitrogen and stored at (−80°C) until extraction of DNA using the DNeasy Blood and Tissue Kit (cat. 69504 QIAGEN, Germantown, MD, USA) combined with QIAshredder (cat ...
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bioRxiv - Microbiology 2021Quote: ... Buffer G2 and proteinase K were incubated at 56°C overnight and extracted using the EZ1 DNeasy Blood Tissue Kit (Qiagen GmbH, Hilden, Germany) in line with the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... Efficient electroporation was confirmed by observation of fluorescence before genomic DNA extraction around 16 hpf (18°C) using QIAamp DNA Micro kit (Qiagen, German Town, MD). Mutagenesis efficacy of individual sgRNAs ...
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bioRxiv - Neuroscience 2021Quote: ... The mixture was incubated at 37 °C for various times and the DNA was purified using a MinElute PCR purification kit (Qiagen, Germantown, MD, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... strain SPR117 was grown during 7 days in R2A broth at 20 °C and its genomic DNA (gDNA) was extracted using the Dneasy UltraClean Microbial Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Brain parts were stored in 100 µL of Trizol™ in -80 °C for later RNA extraction using RNAeasy Mini Extraction Kit™ (Qiagen, Germany) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2019Quote: ... from the bacterial cell pellet of input library and output libraries stored at −80°C was extracted using QIAamp DNA Mini Kit (Qiagen, Valencia, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... individuals were flash frozen in liquid nitrogen and stored in −80°C until DNA extraction using the Qiagen DNeasy Plant kit (Qiagen, Valencia, CA, USA) as described in (Kainer et al ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2024Quote: Total bacterial DNA was extracted from frozen (−80°C) fecal samples from AuPhos- or vehicle treated mice using PowerFecal DNA/RNA Kit (Qiagen, Hilden, NRW, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were kept at −80°C until performing the DNA extraction using DNAeasy Blood & Tissue Kit (QIAGEN; Cat. No. ID: 69504). Qubit Broad Range double-stranded DNA assay (Life Technologies ...
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bioRxiv - Physiology 2023Quote: Isolated human LGR5+ cells were stored at −80°C until processed for RNA isolation using RNeasy micro kit as per manufacturer instruction (Qiagen, Germantown, MD, USA). RNA processing ...
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bioRxiv - Microbiology 2023Quote: ... approximately 1 x 104 colonies were collected and pooled into LB-amp culture media and then incubated at 37°C for five hours prior to harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next generation sequencing (see below) ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 min, 4°C) prior to incubation (1 h, 4°C) with 4.0 mL of Ni-NTA agarose (Qiagen) pre-equilibrated in the same buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the supernatant incubated 1 hr at 4°C with 1.5 ml packed Ni-NTA Agarose beads (Qiagen). Beads were washed with buffer H adjusted to 50 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... Extract was cleared by centrifugation at 186,000g for 1 hour at 4 °C and then incubated at 4 °C with NiNTA resin (QIAGEN) for 4 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Animals were raised at 25°C for 48 h after hatching and total RNA was purified by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Microarray and the following data analysis were performed as described before (Qi et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Enzyme was inactivated at 95 ° C for 10 min and 700 μl Trizol was added followed immediately by RNA extraction using the miRNeasy kit (Qiagen Inc., Valencia, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Both the input and IP samples were incubated at 50°C for 2 hr and then cleaned up using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany, Cat # 28104) and eluted in 35 μL of water.
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bioRxiv - Evolutionary Biology 2019Quote: ... with 5 µl proteinase K (20 mg/ml) and lysed overnight at 56°C under shaking in Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with proteinase K (20 mg/ml) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was prepared from synchronized day one adult animals raised at 20°C by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Oligo-dT primer was used to synthesize cDNA by using Transcription First Strand cDNA synthesis kit (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... All soil samples were stored at –20°C until rhizosphere DNA was extracted using a QIAGEN DNeasy PowerSoil Kit (Qiagen, Inc., Valencia, CA, USA) following the manufacturer’s protocol.
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... The temperature was increased from 25 °C to 95 °C at 3 °C per minute and fluorescence was measured at 610 nm in a Rotor-Gene Q thermocycler (Qiagen). The experiment was performed in technical triplicates ...
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bioRxiv - Molecular Biology 2019Quote: ... were frozen at −80°C and subsequently digested at 56°C overnight by Proteinase K (10mM in Tris-HCl, 19133, Qiagen). To account for potentially differential cell proliferation rate between treatments all the ATP quantifications were normalized to DNA content in the same samples ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Immunology 2021Quote: Pathway-specific primer mixes (Rat Antibacterial Response, PBR-148Z, and Human Antibacterial Response, PBH-148Z; Qiagen) were used for preamplification and qPCR arrays (Rat Antibacterial Response ...
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bioRxiv - Genomics 2022Quote: An aliquot of 150 μL of plasma per sample was thawed on ice and centrifuged at 3000 × g for 5 min at 4 °C and smallRNA was extracted using miRNeasy Serum/Plasma Kits (Qiagen, Cat. No. 217184, Milano, Italy) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Immunology 2023Quote: ... The remaining cells were pelleted by centrifugation (4000rpm, 30min, 4C) and stored at –20°C for bulk EEV extraction by endotoxin-free maxiprep (Qiagen EndoFree Maxi Kit, Cat. 12362).
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bioRxiv - Genomics 2019Quote: ... and stored at −20°C until midiprepped (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2024Quote: ... of Gibson Assembly reaction mix was added to NEB Stbl cell (C3040) for transformation and grown at 30°C or 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled ORC were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled Cdt1 were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer D with 10 mM imidazole for 1.5 hr with rotation at 4°C ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... B220 (RA3-6B2) and Ly-6C/G (RB6-8C5) and anti-rat IgG-coupled magnetic beads (Qiagen) as previously described [34] ...
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bioRxiv - Immunology 2021Quote: ... Serum was stored at −80°C as well as tissue samples were stored at −80°C or in RNAlater (Qiagen, Hilden, Germany) at −20°C.
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bioRxiv - Cancer Biology 2021Quote: ... PCOs were stored at -80°C in RNAlater (Qiagen) until library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... and further disrupted by TissueLyser II (QIAGEN, C.0659). 100 mg ground sample was first resuspended in 1 mL lysis buffer (1×PBS ...
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bioRxiv - Genomics 2020Quote: ... Obtained fully tryptic peptides were mapped to the secalin and nsLTP sequences in CLC Genomics Workbench v12 (Qiagen, Aarhus, Denmark) using 100% sequence identity to confirm the expression at individual protein levels.
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bioRxiv - Developmental Biology 2022Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Ensembl Rnor_5.0.78) using CLC Genomics Workbench 12.0 (Qiagen, Germantown, MD). Transcript abundance was expressed as transcript per million mapped reads (TPM ...
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bioRxiv - Developmental Biology 2023Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Ensembl Rnor_5.0.78) using CLC Genomics Workbench 12.0 (Qiagen, Germantown, MD). Transcript abundance was expressed as reads per kilobase of transcript per million reads mapped (RPKM ...
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bioRxiv - Immunology 2020Quote: ... were either frozen at −80°C in RLT buffer (Qiagen) for future RNA extraction or snap frozen using O.C.T.™ and preserved at −80°C ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Biophysics 2019Quote: ... The cells were transiently transfected with cDNA encoding the rat TRPV2 and its mutants using the Effectene reagent (Qiagen) 48-72 hours before experiments ...