Labshake search
Citations for Qiagen :
151 - 200 of 10000+ citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The indicated cell types were harvested and stored at −80°C until RNA was isolated using RNeasy kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: DNA isolation was performed by adding proteinase K and incubation at 56°C using QIAamp DNA Mini Kit (Qiagen). DNA was quantified using a Qubit Fluorometer (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... stored at −80°C) and PBMCs were extracted using QIAGEN DNeasy Blood and Tissue kit (Qiagen Sciences, Maryland, USA), following manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from a bank of frozen brains kept at −80 °C using a RNA isolation kit according to the manufacturer’s instructions (Qiagen). Frozen brain samples were thawed and dissected from 5 different regions ...
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bioRxiv - Microbiology 2021Quote: ... Membrane filters were stored at −80°C until DNA could be extracted using a DNeasy Power Water kit (Qiagen) and bacteria were quantified using qPCR of the vvhA gene.
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by 5 minutes of centrifugation at 16.000 g of bacterial lysate was stored at −80 °C or immediately purified with the miRNeasy mini kit (Qiagen). RNA quantity was determined using the Qubit 4 Fluorometer and Nanodrop spectrophotometer (Thermofisher) ...
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bioRxiv - Microbiology 2022Quote: ... and stored pellets at −80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s instructions (protocol #5) ...
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bioRxiv - Microbiology 2019Quote: DNA was extracted from subsamples frozen at -20°C using the DNeasy PowerLyzer PowerSoil kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions with minor modifications to increase yield and final DNA concentration based on the assumption that some sites and depths would have a relatively low microbial biomass ...
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bioRxiv - Microbiology 2019Quote: ... with 0.5% NaCl at 30 °C for overnight and DNA extraction was done by DNeasy Blood and Tissue Kit (QIAGEN). Specific forward and reverse primers targeting each gene of interest were used for PCR amplification (TABLE 2) ...
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bioRxiv - Immunology 2019Quote: ... and transferred to −80°C storage until RNA purification with the Qiagen AllPrep RNA/DNA kit (Qiagen, Hilden, Germany). For generating stimulated PBMC samples ...
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bioRxiv - Plant Biology 2019Quote: Genomic DNA was extracted immediately upon UV-C exposure (time point 0) using the Plant DNA Extraction kit (Qiagen). Five µg of genomic DNA were sonicated (Diagenode Bioruptor ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C and genomic DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). The concentration of resultant DNA was determined using a Qubit double-stranded DNA BR assay kit and a Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... total RNA was extracted from the frozen mite samples (−80°C) using the RNeasy Plus Mini Kit (Qiagen, Belgium) according to the manufacturer’s Quick-Start Protocol ...
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bioRxiv - Microbiology 2022Quote: ... The aqueous phase was separated by centrifugation at 13,000 g at 4°C and RNA was purified using the RNeasy Mini Kit (Qiagen) with on-column DNAse digestion ...
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bioRxiv - Immunology 2023Quote: ... 40mM Tris-HCl ph 6.5 for 2 hour at 45°C prior to purification with QIAquick PCR Purification Kit (Qiagen). For ‘input’ DNA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (3,000 g, 15 min, 4°C) and total RNA was isolated using the miRNeasy Mini Kit (QIAGEN) in combination with the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Genomics 2023Quote: ... Transposition reaction was incubated at 37°C for 30 min and purified using the PCR purification MinElute Kit (QIAGEN), according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... we first extracted nuclei from fresh leaves and then prepared libraries using the EpiTect Hi-C Kit (Qiagen, Netherlands). HiC libraries were sequenced on a NextSeq500 instrument (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... for 10 minutes at 37°C followed by a final clean-up with Qiagen RNeasy MinElute Kit (Qiagen, #74204) using default manufacturer’s protocol (which keeps RNAs > 200nt ...
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bioRxiv - Microbiology 2023Quote: ... 37 °C) of strain GFKo1 grown in nutrient broth (Oxoid) using the Qiagen DNeasy Blood and Tissue Kit (Qiagen). Extracted DNA was adjusted to a concentration of 0.2 ng/μL and treated using the Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... cholerae N16961 cultures grown in LB at 37°C in both exponential (OD600 0,8) and stationary (OD600 2,8) growth phases using the RNeasy® Mini Kit (QIAGEN), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µl NEB2 buffer and 0.1 U enzyme were incubated 15 min 30°C and purified from unincorporated nucleotides using Nucleotide Removal Kit (Qiagen). Binding reactions were carried out in a total volume of 10 µl ...
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bioRxiv - Microbiology 2023Quote: ... cholerae WT and ΔSI cultures grown in LB at 37°C in both exponential (OD600 0,8) and stationary (OD600 2,8) growth phases using the RNeasy® Mini Kit (QIAGEN), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... for 1h at 37 °C (2 units per sample) followed by purification with the RNeasy miniElute Cleanup kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... All samples were stored at -80°C until the DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen). A blank DNA extraction was included to account for possible contaminations ...
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bioRxiv - Molecular Biology 2021Quote: ... The peptide-coupled proteins were separated from uncoupled proteins using Ni-NTA Agarose (Qiagen).
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Bioengineering 2022Quote: ... and native rat tissue samples were homogenized in lysis Buffer RLT (Qiagen). Total RNA was isolated using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with goat anti-rat beads (QIAGEN, cat. no. 310107). For intracellular staining ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested by centrifugation at 4°C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Inoculants from glycerol stocks or stab culture were cultured overnight in liquid LB at 37°C and plasmids were extracted using Plasmid miniprep kit (Qiagen). See Key Resources Table for shRNA identity ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 200,000 g for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were incubated at 37 °C for 16 hours overnight and were in turn cleaned-up using the QIAquick PCR Purification kit (QIAGEN) and eluted with 40 μl of 50 °C water ...
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bioRxiv - Microbiology 2019Quote: ... snap frozen on dry ice and stored at −80°C until RNA purification with the Qiagen AllPrep RNA/DNA kit (Qiagen). Immunoglobulin amplicon preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Five colonies with the correct sized insert were inoculated into liquid LB supplemented with spectinomycin and chloramphenicol and grown overnight at 37°C for plasmid extraction using a QIAprep Spin Miniprep Kit (Qiagen). The plasmid constructs were confirmed by Sanger sequencing ...
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bioRxiv - Genomics 2020Quote: ... We stored tissue at 4 °C for 24-72 hours prior to extracting DNA with a DNAeasy Blood and Tissue Kit (Qiagen) with on-column RNase A treatment following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Tissue samples were snap frozen in liquid Nitrogen and stored at −80 °C until genomic DNA (gDNA) was extracted using DNeasy blood and tissue kit (QIAGEN). Tissues were lysed in 360 µL of lysis solution on a Precellys 24 homogenizer for 30s at 4.0 ms−1 ...
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bioRxiv - Genomics 2019Quote: ... transposition was performed on 25,000 sorted tetraploid nuclei at 37°C for 30 minutes followed by DNA purification with the MinElute Reaction Cleanup Kit (28206, QIAGEN). DNA fragments were PCR preamplified for 5 cycles initially ...
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bioRxiv - Neuroscience 2019Quote: ... EST plasmids were grown in Luria Bertani (LB) media overnight at 37°C and purified before further use (QIAprep Spin Miniprep Kit, Qiagen). Plasmids were linearized with PauI (New England Biolabs ...
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bioRxiv - Genetics 2019Quote: ... After 16 hrs in a 37°C shaker bacteria were harvested and plasmid DNA was isolated using a Megaprep kit (Qiagen). ITR integrity was confirmed by digestion with XmaI as well as with AhdI ...
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bioRxiv - Developmental Biology 2019Quote: ... Cross-linking was reversed by incubation overnight at 65 °C and DNA was purified using a MinElute PCR purification kit (Qiagen). All IP DNA and 1 ng of input DNA were used for library preparation using the ThruPLEX-FD Prep Kit or ThruPLEX DNA-Seq (Rubicon Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcribed mRNAs were DNAse-treated using TURBO-DNAse for 15 min at 37°C and purified using the RNeasy Mini Kit (Qiagen) and quantifed using Qubit™ RNA BR Assay Kit (ThermoFisher ...
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bioRxiv - Systems Biology 2020Quote: ... cultures were harvested in RLT buffer and stored at −80°C before isolation of RNA using the RNeasy Mini Kit (Qiagen). mRNA was isolated from 1 ug total RNA by poly-dT enrichment using the NEBNext Polya ...
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bioRxiv - Genetics 2019Quote: ... Cross-links were reversed at 65°C overnight (16 hrs) and DNA was purified using a PCR purification kit (Qiagen). DNA content was quantified by RT-PCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2019Quote: ... The transposition reaction was performed at 37°C for 30 min and was followed by purification of the samples using the Qiagen MinElute PCR purification kit (Qiagen). Transposed DNA fragments were amplified for 11 cycles using the NEBnext high-fidelity PCR master mix and the Ad1_noMX and Ad2.1-2.6 barcoded primers from (10) ...
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bioRxiv - Cell Biology 2021Quote: The input and immunoprecipitated DNA were incubated in the same buffer with the addition of 20 μg Protease K at 65 °C overnight to reverse crosslinks before purification using a QIAquick PCR Purification kit (QIAGEN). Before qPCR analysis ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...
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bioRxiv - Cancer Biology 2021Quote: ... and storing at −80°C.68 DNA extraction of tumor tissue from FFPE was carried out using QIAGEN FFPE DNA extraction kit (QIAGEN). DNA was extracted from plasma and buffy coat using Macherey-Nagel NucleoSnap and QIAGEN Blood and Tissue kits ...
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bioRxiv - Plant Biology 2021Quote: Plant samples of ∼50 mg were frozen in liquid nitrogen and stored at −80°C prior to RNA extraction with RNeasy Plant Mini Kit and on-column RNase-Free DNase Set (Qiagen). Total RNA (0.5 µg ...