Labshake search
Citations for Qiagen :
301 - 350 of 10000+ citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80 °C until clean up using a kit (Qiagen 74004). cDNA was generated using 11 rounds of amplification with 10 ng RNA input ...
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bioRxiv - Cell Biology 2020Quote: Isolated Chromatin was digested with 1% proteinase K for 2 hrs at 60 °C with 300 rpm and was subsequently purified by using QIAquick® Nucleotide Removal Kit (28306, Qiagen) according to the manufactory’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged at 5000 g for 2 hours at 4 °C and the pellet was collected for DNA extraction with a DNeasy PowerSoil kit (Qiagen, Germany). The sediment from Lime Blue was collected with a freeze core (modified from Stocker and Williams ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The right pectoral fin was removed and stored in 95% ethanol at −20 °C prior to subsequent DNA extraction using QIAamp Fast DNA Tissue kits (Qiagen, Inc.) following the manufacturer’s protocols.
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bioRxiv - Genomics 2022Quote: ... After overnight incubation at 37 °C colonies were picked for mini-prep cultures and mini-preps were performed using the Qiaprep® Miniprep kit (Qiagen). The insert in the resulting plasmid was sanger sequenced (Source Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... Islets were pelleted and stored at −80°C until DNA extraction using the Blood/Tissue DNeasy kit (Qiagen; Germantown, MD, USA) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The chromatin fragments were then left to reverse crosslink overnight at 65°C with shaking and the DNA was then purified with the QIAquick PCR purification kit (QIAGEN; 28104), according to manufactures instructions ...
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bioRxiv - Neuroscience 2023Quote: ... After protein digestion (PK Buffer and Proteinase K (20 mg/mL) for 2 hrs at 45°C) DNA was purified with QIAquick PCR Purification Kit (Qiagen, Germany) according to manufacturer instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant was discarded and the pellet was frozen at -20 °C for further extraction with the DNeasy® Plant Mini Kit (Qiagen). Total DNA was eluted in 10 µl of water and concentration was measured using NanoDrop ...
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bioRxiv - Systems Biology 2023Quote: ... Liver samples were stored at -140°C and prepared as follows: total RNA was extracted using miRNeasy kit (Qiagen; Hilden, Germany). Libraries were prepared using 10 ng/μl with Truseq Stranded RNA ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were incubated at 37°C for 30 min and then purified using the MinElute Reaction Cleanup kit (Qiagen Cat #28204) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and incubated at room temperature for 20 min Samples were then frozen at -20°C overnight prior to RNA extraction using the QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen, Cat #28206). Library preparation was performed on purified fragments using Nextera DNA Flex Library Prep Kit (Cat #20018704 ...
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bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown in LB at 37°C with shaking and genomic DNA was isolated using the DNeasy® Tissue Kit (Qiagen) from exponential and stationary phase cultures ...
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bioRxiv - Neuroscience 2023Quote: ... Transposition occurred at 37°C for 30 minutes and transposed DNA was purified using the MinElute PCR Purification Kit (Qiagen, 28004). Indexing libraries using Nextera i5 and i7 indexed amplification primers (Illumina XT index kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted from fresh specimens stored at -20° C in 95% ethanol with a Qiagen DNeasy kit (Qiagen, Hilden, Germany) following manufacturer’s protocols modified by the recommendations of Cruaud et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... two independent limbs were microdissected and snap-frozen at −80°C for subsequent total RNA extraction using the RNEasy Mini Kit (QIAGEN, 74134) following protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The siRNA targeting rat Psmc1 included Rn_RGD:621097_1 FlexiTube siRNA (Qiagen, Cat# SI02002427) and Rn_RGD:621097_2 FlexiTube siRNA (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from cultured rat embryonic striatal neurons following stimulation (RNeasy, Qiagen) and submitted to the Genomic scorelab at the Heflin Center for Genomic Sciences at the University of Alabama at Birmingham for library preparation as previously described17,47 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Colonies that grown on the LB-antibiotic plate were picked and inoculated into fresh LB-antibiotic medium at 37 °C to prepare overnight culture for plasmids extraction using QIAprep Spin Miniprep kit (Qiagen, Hilden, Germany). The plasmids were then sent for DNA sequencing (1st BASE ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were placed at 37°C for 30 min and the resulting fragmented DNA was purified using the Minelute Cleanup Kit (Qiagen, Hilden, Germany), with elution performed in 10 µl of the provided elution buffer ...
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bioRxiv - Genomics 2019Quote: ... 4°C) the aqueous phase was mixed with 700 μl 70% ethanol and applied to a Silica column (Qiagen RNAeasy Mini kit). RNA was then purified according to the manufacturers recommendations ...
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bioRxiv - Immunology 2021Quote: ... The dried DNA pellet was reconstituted in 50 μL H2O treated with 330 μg/mL of RNase A for 2 hours at 37°C and then recovered using the QIAquick PCR Purification kit (QIAGEN Cat#28104). DNA concentration was determined by Qubit (Thermofisher).
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bioRxiv - Microbiology 2022Quote: ... aeruginosa cells were lysed after 18 hours using 2.5mg/mL lysozyme and incubated at 37°C for 30 minutes and RNA was extracted using the RNeasy mini kit (QIAGEN, Germantown, MD). DNase treatment was done twice for better DNA removal using Qiagen RNase-free DNase during RNA extraction and using NEB DNase after RNA extraction ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 12-d-old seedlings grown at 22°C in LD conditions at ZT13 with the RNeasy® Plant Mini Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... was linearized with BbsI-HF restriction enzyme for 1 hour at 37°C and gel purified with QIAquick® Gel Extraction Kit (Qiagen, 28704). Two gRNAs targeting ITGBβ1 (Horlbeck et al. ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: Cells were lysed in RLT PLUS buffer and stored at -80°C until RNA isolation with the RNeasy Micro Kit (Qiagen, Hilden, Germany). cDNA was generated using the iScript Advanced cDNA generation kit (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2019Quote: Total DNA was extracted from tadpole tails by overnight digestion in 10% proteinase K solution at 56 °C and extracted using the Qiagen Biosprint 96 DNA blood kit (Qiagen, CA, USA). DNA was eluted in 100 - 200 μL buffer AE (QIAgen).
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bioRxiv - Biochemistry 2020Quote: Total RNAs were extracted from Wildtype MIN6 and MIN6 stably expressing shRNAs (non-specific/NS, C, D) using RNeasy kit (QIAGEN, Germantown, MD) and cDNAs were prepared using qScript cDNA SuperMix (Quantabio ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Immunology 2020Quote: Fecal pellets were placed in a sterile round-bottom tube and stored at −20 °C until processed for DNA extraction using the QIAamp DNA Stool Mini Kit (Qiagen, Germantown, MD). DNA concentrations determined spectrophotometrically (Molecular Devices ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells were cultured at 37°C and plasmid isolation was carried out using a Qiagen Plasmid Mini Kit (Qiagen, Germantown, MD) according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... All collected plant tissues were frozen immediately in liquid N2 and stored at −70°C until the RNA was extracted and isolated using the RNeasy mini kit (Qiagen; Hilden, Germany) and library prepped using the SQK-PCS109 kit (Oxford Nanopore Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Colonies were selected and grown in liquid broth (Fisher, catalog #12780052) overnight at 37 °C for DNA isolation (by QIAprep Spin Miniprep Kit, Qiagen, catalog #27106) and sequence verification (EuroFins Genomics).
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total mRNA was extracted from lung tissue kept in RNAlater stored at -20 °C using a RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: DNA extraction from 0.25 g rhizosphere soil (frozen at −80°C) was performed using the DNeasy PowerSoil Pro kit (QIAGEN GmbH, Hilden, Germany) according to manual ...
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bioRxiv - Developmental Biology 2022Quote: ... Tubes were placed at 37°C for 30 min and the resulting fragmented DNA was purified using the Minelute Cleanup Kit (Qiagen, Hilden, Germany), with elution performed in 10 µl of the provided elution buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The dissected tissues were immediately placed on dry ice and stored at −80°C until RNA extraction was performed using the RNeasy Plus Micro Kit (Qiagen Catalog # 74034). Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation ...
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bioRxiv - Genomics 2022Quote: ... we extracted total RNA from eight snap-frozen tissues kept at -80°C (brain, breast muscle, heart, kidney, liver, lung, spleen, and testis) using the RNeasy Mini Kit (Qiagen; Hombrechtikon, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was treated with proteinase K at 42°C for 2 hrs and purified using MinElute PCR Purification Kit (Qiagen, Cat# 28004).
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bioRxiv - Microbiology 2023Quote: ... we dried both washed lichen fragments and wash-water pellets at 65°C and extracted DNA with the DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany).
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bioRxiv - Microbiology 2024Quote: ... DNA was precipitated with 100% ethanol and 3 M sodium acetate at -80°C for 60 minutes and then cleaned using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA) per manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 100,000 g (31,000 RPM) for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen, Germantown, MD) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Cancer Biology 2024Quote: ... and AR DBD domain DNA fragment were cut by NheI (N-terminal) and XhoI (C-terminal) and purified with the QIAquick Gel Extraction Kit (Qiagen; cat. 28704). After ligation with T4 DNA Ligase (New England ...