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Citations for Qiagen :
251 - 300 of 10000+ citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... overnight incubation at 37 °C) and DNA was extracted using the QiaAmp Blood Mini Kit (Qiagen Cat No./ID: 51304) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... primers with an annealing temperature of 60 C for 45 seconds and PCR products purified via the QIA Quick PCR Purification Kit (Qiagen). DNA concentration was measured on the Nanodrop ...
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bioRxiv - Biochemistry 2023Quote: ... Filters were incubated at 55°C for approximately 4 h and the resulting lysates were purified with the DNeasy kit (Qiagen) using a slightly modified protocol49 ...
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bioRxiv - Cancer Biology 2023Quote: ... The material stored in Qiazol was heated at 65°C for 5 min and RNA was extracted using the RNeasy Lipid Tissue Kit including the optional DNase digestion (Qiagen). RNA quality and concentration were assessed using a 2100 Bioanalyzer (Agilent Technologies ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following complete removal and evaporation of residual ethanol (10 mins at 30°C) DNA was extracted using the AllPrep DNA/RNA FFPE Kit (Qiagen). DNA was eluted in 40 µl Elution buffer.
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bioRxiv - Cell Biology 2023Quote: ... gDNA wipeout buffer was used to remove Genomic DNA for 2 minutes at 42°C and cDNA was synthetized with the QuantiTect Reverse Transcription Kit (Qiagen). qPCR was performed with PowerUp SYBR Green Master Mix using the StepOnePlus Real-Time PCR system ...
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bioRxiv - Cancer Biology 2023Quote: ... by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Microbiology 2023Quote: ... Template DNA was degraded using RQ1 DNAse for 30 min at 4°C and RNA was purified using RNAeasy mini kit (Qiagen). 4-week-old NRG mice were injected intra-hepatically using 10 µg of RNA in a maximum volume of 50 µL of PBS+RNA and one week post injection a terminal bleed was performed ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen). Extracted gDNA was stored at −20°C.
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Zoology 2024Quote: ... involved proteinase K digestion at 55°C using 20 µl of proteinase K and 220 µl of ATL lysis buffer (MinElute Reaction Cleanup kit, Qiagen). Prior digestion ...
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bioRxiv - Systems Biology 2024Quote: DNA for Nanopore sequencing was purified from frozen samples stored at -80 °C using a Blood & Cell Culture DNA Midi Kit (Qiagen). Approximately 100 mg of tissue sample was crushed in a BioPulveriser cooled in liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... shaking overnight at 30°C for 16hrs and the plasmid DNA is purified using a QiaPrep 96 column DNA purification kit (Qiagen). This procedure was conducted twice on different days to produce two separate replicates.
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bioRxiv - Immunology 2023Quote: ... The supernatant was aspirated out and the pellets stored at −80°C until mRNA extraction using the RNEasy Micro Kit (Qiagen). Corresponding cDNA was synthesized from the mRNA samples using the cDNA amplification kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow of transplanted tumor bearing CTR and VEGF-C mice was isolated and gDNA was purified using QIAmp DNA Mini Kit (Qiagen) as described ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from five-day-old green seedlings with or without four hours of 27°C treatment of each genotype using the RNeasy Plant Kit (Qiagen). For mRNA sequencing (mRNA-seq ...
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bioRxiv - Microbiology 2023Quote: ... an unbiased amplification method was employed by amplifying the purified DNA at 30°C for 8 hours using a REPLI-g Single Cell kit (Qiagen). The amplified DNA was purified using AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Digests were incubated for 2 hours at 37°C then purified using a QiaQuick PCR purification kit (Qiagen Cat#28106). Fragments of 2100 bp were size-selected using a SageELF instrument (Sage Science ...
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Phenogenomic resources immortalized in a panel of wild-derived strains of five species of house micebioRxiv - Evolutionary Biology 2023Quote: Genomic DNA was isolated from frozen (−80 °C) or alcohol-preserved muscle or spleen tissues using DNeasy Blood & Tissue Kits (Qiagen). High-quality DNA aliquots were next-generation sequenced at the Edinburgh Genomics facility using HiSeq X technology ...
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bioRxiv - Microbiology 2024Quote: ... Samples were collected and frozen at −20°C until DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen) according to the Protocol ...
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bioRxiv - Immunology 2024Quote: ... Cytosolic fractions were prepared by centrifugation at 10,000 × g for 30 min at 4°C and DNA was isolated from them using the DNeasy Blood & Tissue kit (Qiagen). The copy number of mitochondrial DNA encoding 16S RNA (RNR2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysed cell material was stored overnight at −80 °C before total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). RNA isolation and RT-qPCR was performed by the University of Rochester Genomics Research Center ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Developmental Biology 2023Quote: ... These were stored at -80°C before RNA extraction using Pre cellys bead homogenisation (Bertin Technologies, France) and RNA easy Kit (Qiagen). Turbo DNA free DNase kit (Ambion ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from the cells dissociated with C&H or dispase or from fresh frozen tissues using RNeasy plus mini kit (#74134, Qiagen). 500 ng of RNA from each sample was used for cDNA synthesis using High-capacity RNA-to-cDNA kit (#4387406 ...
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bioRxiv - Genomics 2024Quote: ... were heat killed in a 95°C water bath for 20 min to produce thermolysates before DNA extraction (MagAttract kit, Qiagen) and whole-genome sequencing ...
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bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated at 37 °C for 30 min followed by immediate purification of DNA fragments with the MinElute PCR Purification Kit (Qiagen). ATAC-seq including amplification of Library and Indexing was performed as described elsewhere67 ...
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bioRxiv - Microbiology 2024Quote: ... 140 μL virus supernatant was treated with 0.25 μg RNaseA for 30 min in 37 °C to remove the extracellular RNA and continued with viral RNA extraction (QIAamp Viral RNA Kits, Qiagen) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 140 μL virus supernatant was treated with 0.25 μg RNaseA for 30 min in 37 °C and continued with viral RNA extraction (QIAamp Viral RNA Kits, Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fecal pellets were collected and stored at −80°C until DNA extraction with DNeasy PowerLyzer PowerSoil Kit (Qiagen; 12855-100). Fecal Rg abundance was determined with Rg-specific 16S rDNA primers ...
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bioRxiv - Genomics 2021Quote: ... and grown at 30°C or 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Developmental Biology 2021Quote: ... Luciferase reporter constructs were either mock-treated or methylated in vitro with SssI CpG methyltransferase for 4 h at 37 °C and purified with the QIAquick Purification Kit (QIAGEN 28704). Reporter plasmid (500 ng ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The reaction was incubated at 37°C for 30 min and purified using a MinElute Reaction Cleanup Kit (Qiagen, Germantown, MD). The PCR reaction consisted of 30 μl transposed DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Bacterial cells were pelleted by centrifugation at 6,000 RCF at 4°C for 15 minutes and plasmid DNA was extracted using a Qiagen Plasmid Plus Midi Kit (Qiagen #12943). To estimate the total number of unique barcodes present in the plasmid pool ...
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bioRxiv - Genomics 2019Quote: ... The fresh blood sample was immediately brought to the laboratory at 4 °C and genomic DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... incubated overnight at 37°C in L-broth containing 50 ng/μL ampicillin and DNA isolated using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... treated with DNase I at 37°C for 30 min and finally cleaned with the miniElute kit (QIAGEN, Catalog Number: &28004). For next-generation sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: We homogenized tongue or ear tissue (stored at −20°C) and extracted genomic DNA using QIAamp Fast Tissue kits (Qiagen, Inc), with verification via gel electrophoresis (2% agarose) ...
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bioRxiv - Genomics 2021Quote: ... The resultant nuclei were then incubated for 30 min at 37°C with an in-house made Tn5 enzyme and purified with the Qiagen MinElute kit (Qiagen, 28004). PCR reactions for each replicate were performed with 15 cycles using Ad1F and Ad2.2R primers and NEBNext High-Fidelity 2X PCR Master Mix (New England Labs Cat #M0541) ...
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bioRxiv - Molecular Biology 2021Quote: ... the PCR products were held at 4°C until PCR purification using the QIAquick PCR purification kit (#28106, Qiagen, Hilden, Germany). DNA concentrations were determined using NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... harvested and stored at −80°C in RLT buffer with β-mercaptoethanol from the RNeasy Mini prep Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions until cDNA synthesis for RT2 Profiler PCR array ...
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bioRxiv - Microbiology 2021Quote: ... Fecal pellets were snapfrozen and stored at −80°C until DNA extraction with the DNeasy Powersoil kit by Qiagen (Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Transposition was performed for 30 minutes at 37°C after which the transposed DNA was purified using MinElute PCR Purification Kit (Qiagen, 28004) and eluted in 10 µl of elution buffer (EB ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Microbiology 2019Quote: ... the supernatant was removed with a micropipet and 350 µL of 4°C Buffer RLT Plus (from Qiagen RNeasy Mini Kit) was added to the pellet to collect the RNA ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2019Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80°C until clean up using a kit (Qiagen 154025593). cDNA was generated using 11 rounds of amplification with 10 ng RNA input.