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Citations for Qiagen :
3701 - 3750 of 6611 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The released genomic DNA was amplified using the REPLI-g Single Cell Kit (QIAGEN) and analyzed by PCR targeting three genes that were polymorphic between the IIdA19G1-GD and IIdA20G1-HLJ isolates ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: Total cellular RNA was extracted from cells with a RNeasy Micro Extraction Kit (QIAGEN). Reverse transcriptase reactions were performed using an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by single-cell RNA purification (Norgen kit) including on-column DNAse I (Qiagen) treatment for 10 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were sorted into 384 well plates each containing 3 μl Vapor-Lock (Qiagen) in all wells and eight different conditions of 0.3 μl lysis buffer consisting of 0.125μM OligodT30VN (5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’ ...
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bioRxiv - Genomics 2024Quote: ... total RNA was isolated from cell lysates using AllPrep DNA/RNA Mini Kit (QIAGEN). RNA quality was assessed based on RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from cells using RNeasy® Plus Mini Kit (74034, Qiagen) and RT was performed with 1-2 μg RNA using SuperScript™ IV VILO™ Mastermix (11756050 ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... We homogenized the flies from each sample in Gentra Puregene Cell Lysis Solution (Qiagen) with ceramic beads using the TissueLyser (Qiagen Inc.) ...
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bioRxiv - Immunology 2024Quote: ... cells were collected and lysed using RNeasy Micro Kit RLT buffer (Qiagen, Hilden, Germany), then passed through Chromatrap gDNA removal columns (Porvair Sciences ...
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bioRxiv - Genomics 2024Quote: ... and purified with a Blood & Cell Culture DNA Kit (Cat# 13343, Qiagen, Beijing, China) following the manufacture’s protocols ...
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bioRxiv - Immunology 2024Quote: ... we extracted DNA from untouched and nucleofected cells using QIAamp DNA Mini Kit (Qiagen). Next ...
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bioRxiv - Immunology 2024Quote: ... and FO cells from three healthy individuals using the RNeasy plus Micro Kit (Qiagen). RNA-seq libraries were prepared as previously described [47] ...
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bioRxiv - Immunology 2024Quote: ... DNA was extracted from CRISPR edited cells using DNsasy Blood & Tissue Kit (QIAGEN, 69506). DNA samples were PCR amplified using KOD Hot Start DNA Polymerase (MilliporeSigma ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from Vero rKSHV.294 cells using the RNeasy kit (Qiagen) as per manufacturer’s suggestions ...
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bioRxiv - Cancer Biology 2024Quote: DNA isolation was performed using the Blood and Cell Culture DNA Mini Kit (QIAGEN). The isolated DNA samples were sent to the Center for Applied Genomics at the Children’s Hospital of Philadelphia for the SNP array analysis using the Infinium Global Screening Array-24 v3.0 Kit (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from cell pellets by the QIAamp Blood Maxi Kit (Qiagen) and resuspended in Buffer EB (10 mM Tris–HCl [pH 7.5] ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X106 cells were mixed with 5 μl of 20 μM siRNAs (GeneSolution siRNA, Qiagen) and 100 μl of Ingenio electroporation solution (Mirus ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from human cells using RNeasy Mini kits (QIAGEN, Hilden, Germany) and reverse transcribed using Super-Script III (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and genomic DNA extracted using QIAmp UCP DNA Micro kit (QIAGEN). The targeted loci were PCR amplified with NGS adaptors and sent for amplicon sequencing (Primers Table 1 “Panc480 mutation validation” & 3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... cell pellets were resuspended in Qiagen P2 Lysis Buffer (Qiagen, Hilden, Germany, Cat.#19052) to promote cell lysis ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were genetically validated by extracting the genomic DNA (Blood and Tissue Kit, Qiagen), amplifying the stably integrated gene by PCR and sequencing the PCR product.
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bioRxiv - Genomics 2023Quote: ... pulcherrimus genomic DNA was extracted by using Blood & Cell Culture DNA Midi Kit (QIAGEN), and short genomic DNA segments were removed by Short Read Elimination XL Kit (Circulomics Inc.) ...
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA transfections in HEK293FT cells were performed using Effectene transfection reagent (#301425, QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen). Extracted RNA was used to synthesize cDNA using IgM and IgG reverse transcription primers and SuperScript III enzyme (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Total mRNA was isolated from 5×105 cells using a kit (RNeasy kit, QIAGEN) and reverse-transcribed using oligo(dT)20 primer (SuperscriptIII ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from siNT and siPITAR cells using the Trizol method (Qiagen). RNA quality was assessed using an Agilent TapeStation system ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼500 flow sorted cells were resuspended in 2.5 µl of RLT plus buffer (Qiagen). Polyadenylated RNA was captured with oligo-dT conjugated beads and processed using Smart-seq2 protocol(66) ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNA was extracted from adherent cell layers using the RNAeasy Plus Kit (Qiagen) and reverse transcribed into cDNA using random primers with the Taqman cDNA reverse transcription Kit (Applied Biosciences ...
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bioRxiv - Genomics 2022Quote: ... RNA was isolated from the cells using RNeasy plus micro kit (QIAGEN, Cat# 74034) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated using the Blood and Cell Culture DNA Maxi Kit (Qiagen) and integrated gRNA sequences were amplified by PCR using HiFi HotStart ReadyMix (KAPA) ...
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bioRxiv - Immunology 2023Quote: ... total RNA was isolated from clarified lung homogenates and cultured cells using RNeasy (Qiagen). The RT reaction and qPCR were performed using ReverTra Ace and Thunderbird SYBR (Toyobo) ...
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bioRxiv - Immunology 2023Quote: ... Total RNA of the sorted B cells was isolated with RNeasy Mini Kit (Qiagen). The libraries for RNA-Seq were sequenced using 2×75 bases paired-end protocol on an Illumina HiSeq 3000 instrument ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA samples were extracted from cell pellets using a RNeasy Mini kit (QIAGEN) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... gDNA was extracted from cell culture using the DNeasy Blood & Tissue Kit (QIAGEN, Netherlands).
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bioRxiv - Microbiology 2023Quote: RNA of cells was transcribed to cDNA using the QuantiTect Reverse Transcription Kit (Qiagen): 50-1000 ng RNA were mixed with RNAse-free water and gDNA Wipeout buffer for 2 min at 42°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were collected at OD6000.35 for RNA isolation by using the RNeasy kit (Qiagen). PrimeScript™ RT Reagent Kit (TAKARA ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from sorted cells with a RNeasy plus mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from monolayer cell cultures using miRNeasy Mini kit (Qiagen, Germantown, MD) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... an aliquot of 100,000 cells were pelleted and genomic DNA was extracted (Qiagen, 69506) for PCR and Sanger sequencing ...
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bioRxiv - Systems Biology 2023Quote: ... we isolated gDNA from barcoded cells using the QIAmp DNA Mini Kit (Qiagen #51304) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from cells using the Qiagen RNeasy kit (Qiagen, Valencia CA). Complementary DNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Systems Biology 2023Quote: ... We then isolated gDNA from cells using the QIAmp DNA Mini Kit (QIAGEN, 51304), following the spin protocol for DNA purification from blood or bodily fluids ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from cell lysates and purified with RNeasy Mini Kit (Qiagen) or Direct-zolTM RNA MiniPrep Kit (Zymo Research) ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from cells using the QIAshredder kit (QIAGEN, Redwood city, CA, USA) and the RNeasy mini kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from dHL-60 cells using the RNeasy Mini Kit (Qiagen #74104) with the RNase-Free DNase Set (Qiagen #79254 ...
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bioRxiv - Immunology 2023Quote: ... RNA was isolated from dHL-60 cells using the RNeasy Mini Kit (Qiagen #74104). RNA was converted into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems #43-688-14 ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cultured meningioma cells using the RNeasy Mini Kit (74106, QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cellular RNA was isolated from sorted B cell subsets with RNeasy Micro Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... sorted cells were washed and RNA isolated using the RNeasy Plus Micro Kit (Qiagen) including a gDNA elimination step by QIAshredder spin columns (Qiagen).
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were kept at 37 LJ for 30 minutes and then purified by Qiagen MinElute Kit ...