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Citations for Qiagen :
3901 - 3950 of 6611 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted and purified from cells using QIAamp DNA Micro Kit (Qiagen 56304) with the user-developed protocol QA43 and no more than ~0.5M cells per column ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from the induced harvested cells using the RNeasy Plus Mini Kit (Qiagen). This RNA was used for qPCR to validate the RBP knockdown as well as for semi-quantitative RT-PCR experiments to check the splicing pattern of endogenous CD19 ...
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bioRxiv - Genomics 2019Quote: ... gDNA from PC3 and DU145 cells were purified using the QIAamp DNA Mini Kit (Qiagen) and sonicated to ~170 bp using a Covaris S2 sonicator to create a synthetic DNA template with similar physical properties to cfDNA.
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bioRxiv - Cancer Biology 2019Quote: Melanoma cell lines were collected and RNA isolated with the RNeasy kit (Qiagen, Valencia, CA). After subsequent cDNA amplification using Superscript III (Invitrogen ...
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bioRxiv - Genetics 2019Quote: DNA from whole cell populations was purified using Qiagen DNeasy Blood & Tissue Kit (Qiagen 69504). PCR amplification was performed with MyTaq DNA Polymerase (Bioline BIO-21108 ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from cell culture supernatants using QIAamp Viral RNA Mini Kit (Qiagen) and reverse-transcripted to cDNA using LunaScript RT SuperMix kit (New England Biolabs ...
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bioRxiv - Immunology 2019Quote: Total cellular RNA was isolated from cells using the RNeasy isolation kit (Qiagen, Germantown, MD). The cDNA was synthesized from total RNA using Taqman reverse transcription reagents (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: Total cellular RNA was isolated from cells using the RNeasy isolation kit (Qiagen, Germantown, MD). RNA was quantitated and qualified with Nanodrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2019Quote: Total DNA from cell lines was extracted using the DNeasy Blood & Tissue Kit (Qiagen, Germany). Quantitative PCR was performed using TaqMan™ Universal Master Mix II ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from cell pellets using the DNeasy Blood and Tissue kit (Qiagen, 69504). DNA quantity and quality were measured using a NanoPhotometer (Implen).
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bioRxiv - Genomics 2020Quote: DNA was extracted from cell pellets using the DNeasy Blood and Tissue kit (Qiagen, 69504). DNA quantity and quality were measured using a NanoPhotometer (Implen) ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA was isolated from knockout cell lines using the Gentra Puregene Kit (QIAGEN #158388) and amplified with the primers to yield an amplicon roughly 150-250 base pairs in length using Herculase II Fusion Polymerase (Agilent #600675) ...
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bioRxiv - Molecular Biology 2019Quote: Cells were harvested using trypsin and RNA was extracted according to manufacturer’s instructions (RNeasy, Qiagen). 2 µg of the extracted RNA were reverse-transcribed using the high capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: Total RNAs were extracted from 8×105 cultured cells using RNA isolation kit (Qiagen, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed and resuspended in DM supplemented with RNA protect reagent (Qiagen, Germany). Cell lysis and total RNA extractions were performed with the RNeasy mini kit according to the manufacturer’s recommendations (Qiagen ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen) and sequence verified by Sanger sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from infected cells using QIAamp viral RNA kit (Qiagen, https://www.qiagen.com) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... and CHO-K1 cells respectively using a RNA isolation kit (RNeasy Mini Kit) from Qiagen. cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits ...
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bioRxiv - Genomics 2020Quote: ... The supernatant of cell lysate was incubated with 1 mL Ni-NTA agarose beads (QIAGEN) at 4 °C for 1 h ...
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bioRxiv - Pathology 2019Quote: ... DNA was extracted from tumour cell-enriched areas using DNeasy tissue kit (Qiagen, Hilden, Germany). Multiplex PCRs were performed ...
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bioRxiv - Immunology 2019Quote: We submitted RNA purified from the indicated cell culture conditions using an RNeasy kit (Qiagen) to the QB3-Berkeley Functional Genomics Laboratory ...
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bioRxiv - Bioengineering 2019Quote: Genomic DNA from cells or liver tissues were collected using DNeasy Blood & Tissue Kit (Qiagen). Target sites were amplified using High Fidelity 2X PCR Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was extracted from the cultured cells with the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from CD4+ T cells using the DNeasy blood and tissue kit (Qiagen), according to manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: Genomic DNA was isolated from 1×106 tumor cells using DNeasy Blood & Tissue Kit (Qiagen). BCMA and CS1 loci amplicons ...
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bioRxiv - Genetics 2021Quote: ... Total genomic DNA was extracted from cells using DNeasy Blood and Tissue Kit (Qiagen, USA). Primers flanking the MT-ND4 gene were used to amplify the edited locus using MyTaq polymerase (Bioline ...
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bioRxiv - Genetics 2019Quote: ... We extracted high molecular weight (HMW) DNA using Gentra Puregene Cell Kit (Qiagen, Hilden, Germany) from 5 × 106 cultured cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted DNA from each cell line using the DNeasy Blood and Tissue Kit (Qiagen) and prepared DNA-Seq libraries from 100 ng DNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was prepared from tumor cells using an RNeasy Mini kit (Qiagen; Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cells using QIAshredder and RNeasy mini kits (Qiagen, Hilden, Germany). cDNA was prepared from 1 μg of RNA using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... total mRNA from host cells was isolated using the RNeasy Mini kit (Qiagen, Valencia, CA) following our published standard procedures ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNA was isolated from cells using an RNAeasy Plus Mini kit (QIAGEN Inc, Valencia, CA). One-step qRT-PCR reactions were performed in triplicate using QuantiTech RT mix (QIAGEN ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from bone or bone marrow cells using RNeasy Plus Mini Kit (Qiagen). Four millimeters of fracture calluses free of bone marrow were homogenized for mRNA extraction ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from HCT-116 cells using the RNA-Easy Mini Plus Kit (Qiagen). Likewise ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were transfected with hTRPM3α2-GFP or its mutants using the Effectene reagent (Qiagen). Cells were loaded with 1 μM fura-2 AM (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells by using RNeasy Mini Kit (cat. # 74104) from QIAGEN. Paired-end sequencing was performed using the High-Seq platform with 100bp read length ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from cells using RNA extraction kits (QIAGEN, RNeasy Mini Kit, cat. 74106) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from ovarian cancer cell lines were extracted via RNeasy Mini Kit (Qiagen Inc.) and reverse transcribed by iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... cell pellet was resuspended with 250 μL of buffer P1 (with RNAse added) (Qiagen, 27104) by pipetting up and down ...
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bioRxiv - Bioengineering 2021Quote: RNA was extracted from ∼1 M cells using the QIAGEN RNeasy Mini Kit (QIAGEN 74104). A total of 36 samples were prepared ...
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bioRxiv - Bioengineering 2020Quote: ... The hydrogels and attaching cells were stored in 350 µl RLT lysis buffer (Qiagen, Germany) supplemented with 10 μl/ml β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Total RNAs of sorted cells were extracted by using the RNeasy Mini Kit (QIAGEN #74104) for whole-transcriptome sequencing ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA from transfected cells was extracted using DNeasy Blood and Tissue Kit (Qiagen, #69504).
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bioRxiv - Cancer Biology 2020Quote: Tumor cell RNA was isolated in Trizol and purified using RNeasy columns (Qiagen, Germantown, MD). An initial RNA-seq analysis was performed on a small set of MMs ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA was isolated from 200×106 cells using QIAamp DNA blood midi kit (Qiagen) followed by DNA purification using DNeasy PowerClean Pro Cleanup kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA of cancer cells was extracted with the QIAamp DNA Mini Kit (51304, QIAGEN) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was purified from each DLBCL cell line using DNeasy Blood & Tissue Kit (Qiagen) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: Cell-free DNA (cfDNA) was isolated using QIAamp Circulating Nucleic Acid Kit (Qiagen®, Germany) from different volumes of plasma samples (850µl to 2ml) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and genomic DNA was harvested using Blood & Cell Culture DNA Mini kits (Qiagen, Hilden, Germany). Input samples were harvested from infected but unsorted cells for each replicate.
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and total RNA was extracted using an RNeasy mini kit from Qiagen. cDNA was synthesized using 500 ng of total RNA ...