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Citations for Qiagen :
3551 - 3600 of 6611 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from siRNA treaded cells using the RNeasy Mini kit (Qiagen), 48 and 72 h post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted from cultured cells using the RNeasy RNA Isolation kit (Qiagen 74104) and used as template for cDNA generation with a High-Capacity cDNA Reverse Transcription Kit (Thermo 4368814) ...
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bioRxiv - Genomics 2019Quote: Dissociated human islets cells were transfected with scramble siRNA (Cat# 1027284, Qiagen, Toronto, Canada) or siRNA from ThermoFisher Scientific against OGDHL (ID# s31422) ...
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bioRxiv - Systems Biology 2020Quote: ... RNA was extracted from the harvested cells using the RNeasy®Mini Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... lactis was obtained using the Blood & Cell Culture DNA Mini kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... total mRNA was isolated from Sf21 cells using an RNeasy kit (Qiagen, Hilden, Germany), and reverse-transcribed to cDNA using a Protoscript II First Strand DNA Synthesis kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA of cell lines was extracted using RNeasy RNA extraction kit from Qiagen, (Valencia ...
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bioRxiv - Plant Biology 2020Quote: ... coli Rosetta cells was carried out using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) as described in Barja and Rodríguez-Concepción (2020) ...
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bioRxiv - Systems Biology 2020Quote: Total RNA was extracted from transfected cells using RNeasy Micro Kit (#74004, QIAGEN, Germany). First-strand cDNA synthesis was performed on 500-1000 ng of total RNA ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were thawed and DNA was purified using a DNeasy PowerSoil kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Intracellular HBV DNA was extracted from the cells using the QIAamp Mini Kit (QIAGEN), and the extracellular HBV DNA was recovered from the supernatant using the SideStep Lysis and Stabilization Buffer (Agilent Technologies ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from lung cancer cell lines using the RNeasy Mini kit (Qiagen). The extracted RNA was converted to cDNA using SMART-seq v4 Ultra Low input RNA kit (Takara) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or human ABCB1-transfected MDR-19 cells using the RNeasy Mini extraction kit (Qiagen). First strand cDNA synthesis was performed on 500 ng of template RNA using MMLV reverse transcriptase (fc 10 units/µl) ...
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bioRxiv - Developmental Biology 2021Quote: ... the media was taken off and cells were lysed directly in Qiazol (Qiagen, 79306), followed by RNA preparation.
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bioRxiv - Physiology 2020Quote: DNA was prepared from cells using Gentra Puregene Kits including RNase A treatment (Qiagen). mtDNA copy number per nuclear genome in MSC-derived adipocytes was quantified as described previously (18) ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from infected cells using DNeasy Blood & Tissue mini kit (Qiagen), and then the Pol and Env-coding regions were amplified by PrimeSTAR GXL DNA Polymerase (Takara ...
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bioRxiv - Microbiology 2020Quote: ... cells were harvested for viral RNA extraction with a viral RNA kit (52906, QIAGEN) according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Plasmids of 5 µg were transfected into 6x10[6] S2 cells using Effectene (Qiagen) and incubated for 3 days ...
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bioRxiv - Immunology 2020Quote: ... and small intestine epithelial Trm (CD103hiCD62Llo) P14 cells were sorted into TCL buffer (Qiagen) with 1% 2-Mercaptoethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were processed to total RNA using the RNeasy Plus Mini Kit (Qiagen) according to standard protocols ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was collected from treated cells using RNeasy Mini Kit per manufacturer’s protocol (QIAGEN). RNA integrity was confirmed with a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... Cells in solution were spun down and lyzed using the QIAshredder Kit (Qiagen; 79656). mRNA was harvested using the RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was isolated from transduced cells using a Genomic DNA Purification kit (Qiagen). gDNA was digested with HindIII HF (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... Total DNA was extracted from naive cells with DNeasy® blood & tissue kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Total DNA was extracted from cells using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany), following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was extracted from cells using the RNeasy Plus Mini Kit (Qiagen; 74134) and 1 μg RNA was used for cDNA synthesis with SuperScript II Reverse Transcriptase (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell culture supernatants was extracted using a Viral RNA Mini Kit (Qiagen) and RNA from cell lysates was extracted using a RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA was isolated using the Blood and Cell culture MAXI Kit (Qiagen, 13362), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... the cells were incubated in an alkaline solution (buffer DLB and DTT, Qiagen, Germany) for 5 min at room temperature ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from fresh cells using the RNeasy Plus Mini Kit (Qiagen, 74134). Approximately 1000 ng of extracted RNA was used to prepare libraries for sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Total RNA was isolated from iPSC cells (409B2) using the RNeasy Mini Kit (Qiagen). Coacervates were generated by adding CM-Dex ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were reverse transfected with 5 pmol of each siRNA (QIAGEN) using lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Genetics 2020Quote: Total RNA was isolated from the cells using RNeasy Plus Mini Kit (Qiagen, #74136) according to manufacturer’s protocol and cDNA was synthesized ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was isolated from cell pellets using the RNeasy Mini Kit (Qiagen, 74104). cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were incubated with 100 μl of Ni-NTA superflow beads (Qiagen, 30430) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from spleen-cell suspensions using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Extraction of total RNA from cells was performed using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from S2 cells using the RNeasy Plus Mini Kit (Qiagen 74134). RNA was reverse transcribed using the Maxima H minus system (Thermo Fisher K1681).
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted from total cell lysate and cytosolic fractions using DNeasy kit (Qiagen). DNA samples were each diluted 1:10 and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The pAC5.1-miRNA (300 ng) was transfected into Drosophila S2 cells using Effectene (Qiagen) per the manufactures’ instructions ...
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bioRxiv - Physiology 2020Quote: Total RNA was isolated from C2C12 cells using the RNeasy minikit (Qiagen, Valencia, CA) and reverse transcribed into cDNA using 1 μg of RNA and iScript Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: Total RNA was isolated from the experimental cells using RNAeasy kit (Qiagen, Germantown, MD) as per manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored in either 1 ml Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA), Queen’s buffer ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from cells using the DNeasy blood and tissue kit (Qiagen). Nectin1 deletion was confirmed by PCR amplification of the 5’ region of the NECTIN1 gene (Forward and reverse primers ...
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bioRxiv - Evolutionary Biology 2020Quote: DNA was extracted from WT T87 cells using a DNeasy Plant Mini Kit (QIAGEN). DNA (2.0 μg ...
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bioRxiv - Immunology 2021Quote: ... Genomic DNA was isolated from HEK293T cells using DNeasy Blood and Tissue Kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from cells using by QIAzol Lysis reagent (QIAGEN, CA, USA). Reverse transcription of 2 μg RNA was performed using M-MLV reverse transcriptase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... cells or tissues were lysed using a TissueLyser II (3 min, 30 Hz; Qiagen) in RIPA buffer (150 mM NaCl (Merck) ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µl cell culture supernatant was mixed with 200 µl AVL buffer (Qiagen, Germany) and ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was purified from approximately 40,000 cells using the RNeasy Mini Kit (Qiagen). For total RNA preparation from fixed cells ...