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Citations for Qiagen :
3601 - 3650 of 6611 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: RNA was extracted from the indicated cell lines with the RNeasy kit (Qiagen, 74136) and converted to cDNA using qScript (Quanta ...
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bioRxiv - Systems Biology 2021Quote: ... cell pellets were harvested and mRNA was extracted using the RNeasy Mini Kit (Qiagen). 500 ng extracted total RNA was reverse transcribed into cDNA for each sample ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were FACS-sorted for mCherry+ directly into cytoplasmic lysis buffer (Qiagen Buffer C1), after which the standard Qiagen Blood and Cell Culture DNA kit workflow was carried out for DNA extraction ...
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bioRxiv - Neuroscience 2020Quote: Sequencing libraries were prepared using the QIAseq FX Single Cell RNA Library Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: RNA was isolated from cell sorted GFP+ microglia using the RNeasy Mini kit (Qiagen) per manufacturer’s instructions and eluted in nuclease free water ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen, Germantown, MD) and from tissue (∼50-100 mg cut over dry ice ...
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bioRxiv - Biophysics 2021Quote: ... coli bacteria and isolation from the cells using a QIAprep Spin Miniprep Kit (Qiagen), the plasmid was linearized by restriction enzyme digestion at the last restriction site RS6 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from CD11b-positive cells using the RNeasy Micro Kit (Qiagen). Libraries were prepared using Smart-Seq2 64 and modified for sequencing using the Nextera XT DNA Sample Preparation Kit (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA from sorted cells was isolated using miRNeasy Mini Kit (Qiagen, Cat# 217004). Total RNA from human and mouse primary muscle progenitor cells was isolated using TRIzol/Chloroform standard protocol ...
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bioRxiv - Cell Biology 2020Quote: FAK-WT or FAK -/- SCC cells were transiently transfected using HiPerFect (Qiagen, Manchester, UK), according to manufacturer’s protocol with a final concentration of 80 or 100 nM siRNA respectively (Supplementary Material and Methods ...
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bioRxiv - Genetics 2021Quote: Total RNA from the cell lines was extracted using the RNeasy mini Kit (Qiagen). Total RNA from HNEs or HBEs was extracted using the QIA shredder and RNeasy micro Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: RNA isolation from FACS-sorted cells was performed using the miRNeasy mini kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... putida type strain cells (200 μl) was extracted using MagAttract HMW DNA Kit (Qiagen). The DNA was eluted in 100 uL AE buffer ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from approximately 1×106 cells using the RNeasy Mini kit (Qiagen). DNA was digested using the TURBO DNA-free Kit (Ambion) ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant of the lysed cells was loaded onto Ni-NTA columns (Qiagen, Germany), and the proteins were further purified by a gel-filtration column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: RNA from cell culture supernatant was isolated using the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... nasal swab samples or cell cultured virus isolates by RNeasy Mini Kit (QIAGEN, Denmark) automated on the QIAcube (QIAGEN ...
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bioRxiv - Immunology 2021Quote: ... CD4+ cells were cross-linked and lysed according to EpiTect ChIP OneDay kit (Qiagen). After sonication to shear the chromatin ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from cells using RNeasy Plus Mini Kit (QiaGen, Cat#74134) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: RNA from infected cell supernatants was extracted using QIAamp viral RNA mini kit (Qiagen) and cDNA was synthesized using the High Capacity cDNA RT kit (Thermo ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted and purified from single cell suspensions using RNeasy Micro Kit (Qiagen) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... ligated then isothermally amplified using a QIAseq FX single cell RNA library kit (Qiagen). Fragmentation and dual-index library preparation was conducted with an Illumina DNA Prep ...
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bioRxiv - Immunology 2020Quote: DNA was extracted from the sorted cells by using a DNA-extraction kit (Qiagen) and then bisulfite treated using an EZ DNA methylation kit (Zymo Research) ...
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bioRxiv - Immunology 2020Quote: Total RNA of IFN-α2 treated HEK293 cells was purified using RNeasy columns (Qiagen) with on-column DNase I digestion ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from cell pellets using DNeasy Blood & Tissue Kit (69506, Qiagen). The VZV TK gene (ORF36 ...
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bioRxiv - Immunology 2020Quote: RNA from infected cells was extracted using QIAGEN RNeasy (QIAGEN RNeasy Mini Kit, 74106) following the manufacturers instructions ...
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bioRxiv - Microbiology 2021Quote: ... whole genome amplification was performed using REPLI-g Single Cell kits (Qiagen, Hilden, Germany) [15] ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: Total RNA was prepared from frozen cell pellets using the RNeasy Micro Kit (QIAGEN). RNA was quantified using a Quantus Fluorometer (Promega ...
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bioRxiv - Immunology 2020Quote: ... RNA extraction from sorted cells was performed by using RNeasy Plus Micro kit (Qiagen). The cDNA was generated by employing ingredients from the TAKARA cDNA Synthesis Kit (TAKARA Bio Europe) ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were lysed for RNA extraction as per manufacturer’s instructions (Qiagen, Valencia, CA). T7 oligo(dT ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells and plasmids were isolated using standard miniprep kits (Macheray & Nagel, and Qiagen). Sequences of integrative plasmids can be found in Supplementary Table 4.
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bioRxiv - Genomics 2021Quote: ... All vectors for mammalian cell experiments were purified using Plasmid Plus Midiprep kits (Qiagen), PureYield plasmid miniprep kits (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... An equivalent of OD600 = 3.0 of cells were collected and RNAprotect Bacteria Reagent (Qiagen) was mixed with the cells as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... One fifth of the cells was used for plasmid DNA extraction (Qiagen, Hilden, Germany). The remaining cells underwent RNA extraction using the innuPREP RNA Mini Kit 2.0 (Analytik Jena ...
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bioRxiv - Genomics 2021Quote: ... Transient Transfections of S2 cells were performed using Qiagen Effectene Transfection reagent (Qiagen 30425) at a 1:25 DNA-Enhancer ratio ...
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bioRxiv - Genomics 2021Quote: ... RNA from bulk live cells was extracted using the RNeasy Plus Mini Kit (Qiagen). Cells were first suspended in a combination of the standard Drop-seq lysis buffer with PBS at equal volume ratio (to make the lysis buffer’s final concentration the same as that in nanoliter droplets) ...
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bioRxiv - Neuroscience 2021Quote: ... Microdissected cells were lysed in 100 μl of RNA lysis buffer (Qiagen, Hilden, Germany) and stored at −80°C until further use ...
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bioRxiv - Cancer Biology 2021Quote: RNA was isolated from cells in culture using the RNeasy plus mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cell pellets using the RNeasy Plus Mini kit (Qiagen). RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... were isolated using the “miRNeasy Mini Kit” (cell numbers ≥ 5 × 105, Qiagen, Cat# 217004). FACS-isolated FO or MZ B cells were directly sorted into the Qiazol Lysis Reagent (700 μl final volume) ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was extracted from B cells using RNeasy Mini Kit (Qiagen, Valencia, CA). 100ng RNA was reverse transcribed using SuperScript IV VILO Master Mix (Thermofisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from mouse MLL-AF9 cells by RNeasy Micro Kit (Qiagen), 72 hours after transduction of the cells ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was immediately isolated from SPMCs following cell seeding experiments using RNeasy Microkits (Qiagen). Primers for CFOS ...
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bioRxiv - Immunology 2022Quote: ... Total RNA from sorted cells was isolated with the Rneasy Plus Micro Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... CD4+ cells were cross-linked and lysed according to EpiTect ChIP OneDay kit (Qiagen). After shearing and pre-clearing of chromatin ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed directly with RLT buffer from the RNeasy Mini Kit (74016; Qiagen) followed by RNA extraction following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted from cells using a RNeasy Plus Kit (Qiagen, Cat. #74034) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA from unsorted cell population was isolated using the QIAamp Blood Maxi Kit (Qiagen). For both sorted and the unsorted cell populations ...
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bioRxiv - Molecular Biology 2022Quote: ... serum or cell medium was extracted using the QIAmp DNA Mini Blood kit (Qiagen), used according to the ‘‘Blood and body fluid protocol’’ ...
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bioRxiv - Bioengineering 2022Quote: ... cell lysates were harvested using the RNeasy Mini kit (Qiagen #74104, Germantown, MD, USA), according to the manufacturer’s instructions ...