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Citations for Qiagen :
3651 - 3700 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: From pellets of 100 million cells genomic (g)DNA was extracted using the QIAamp DNA Blood Maxiprep Kit (Qiagen) according to manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2024Quote: ... DNA was isolated from STX4 KO clones and BEAS-2B.Cas9 control cells using the QIAamp DNA Micro Kit (Qiagen) per manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from approximately 5-10 million cells from each organ using the RNeasy Mini Kit (QIAGEN). SuperScript® IV Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Cell pellets were processed for extraction and purification of genomic DNA using DNeasy Blood and Tissue Kit (#69506, Qiagen). Target loci were amplified from purified gDNA by PCR using NEBNext Ultra II Q5 Master Mix (#M0544X ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcriptase reactions were performed on mRNAs isolated from hybridoma cell lines using RNeasy Micro kit (Qiagen, Hilden, Germany), followed by Superscript first strand synthesis reaction (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 20,000 events/cells were sorted directly into RNA lysis buffer and RNA was extracted using RNeay Micro Kit (Qiagen). cDNA synthesis and qPCR were set up as described above ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from cells by using the RNeasy Plus Mini Kit (cat# 74134, Qiagen, Germantown, MD, USA) and cDNA was synthesized and amplified from 1 μg total RNA using the using SuperScript IV VILO Master Mix with ezDNase Enzyme (cat# 11766050 ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA from tumor and normal cells with the indicated treatments was extracted using the miRNAeasy Plus kit (Qiagen) and used for the analysis of gene expression using RT-qPCR (Four replicates ...
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bioRxiv - Genomics 2022Quote: ... Cells were harvested after 24 hours after transfection and total RNA isolated using the RNeasy Maxi kit (Qiagen, 75162) with on-column DNase I digestion ...
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bioRxiv - Microbiology 2023Quote: ... Viral genomic DNA was purified from the harvested cells using a QIAamp DNA Blood mini kit (Qiagen, CA, USA). The viral genome copy number was quantified by qPCR and normalized to the amount of total DNA.
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bioRxiv - Systems Biology 2022Quote: ... genomic DNA was extracted for pelleted cell fractions with one of the following: DNeasy Blood & Tissue Kit (Qiagen #69504) for fractions with fewer than 5 x 106 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... as well as by single cell qPCR for GAPDH or ACTB using the QuantiNova SYBR Green PCR Kit (Qiagen). cDNA concentration was measured using Quant-iTTM PicoGreenTM dsDNA Assay Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from KMS11 or JJN3 cells treated with NT or ILF2 ASOs using the RNeasy kit (Qiagen). Estimates of gene expression were generated by pseudo-aligning FASTQ files against human genome GRCh38.p12 (Ensembl version 94 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then collected via centrifugation and genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). Sequencing libraries were generated essentially as described67 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were normalised to cell density and DNA was isolated using the Plasmid Maxi Kit (Qiagen, Cat. No. 12163) according to manufacturer’s directions except that all steps and reagents ...
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bioRxiv - Microbiology 2022Quote: Nucleic acid from 100µL of cell supernatant were extracted using QIAamp 96 DNA kit and Qiacube HT robot (both from Qiagen).
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bioRxiv - Genomics 2023Quote: Genomic DNA was harvested from post-transfection HuH-7 cells using the DNeasy Blood & Tissue Kit (Qiagen, catalog #69506). Similarly ...
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bioRxiv - Neuroscience 2023Quote: Total RNA of MSN cultures over time was extracted and purified from cell lysates using RNeasy Mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: RNA from the collar cells of regenerating feather buds was isolated using the Qiagen RNEasy Kit (Qiagen, Valencia CA), treated with the Zymo PCR Inhibitor Removal kit (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA from cultured cells was isolated using Qiagen RNeasy Mini Kit according to the manufacture’s protocol (Qiagen, 74106). RNA yield and quality of the samples were assessed using a NanoDrop spectrophotometer ...
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bioRxiv - Immunology 2023Quote: ... and plasmid DNA from 200-500 µl of the cell suspension was purified using QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... total RNA was isolated from 250 cells (or less; see Table S8) using the RNeasy MinElute kit (Qiagen 74204). RNA extraction was performed according to the manufacturer’s protocol with an additional DNase digest step between two washes with 350µl RW1 ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from cell lines or pooled tumor spheroids using the miRNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Bacterial cells were cultured overnight in liquid and the final libraries were extracted using QIAGEN Plasmid Maxi Kit (QIAGEN). The libraries were quantified and subpools combined in 1:4.76 molar ratio to get the final library containing 5,760 gRNAs.
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bioRxiv - Neuroscience 2023Quote: ... Individual microglial cells were cut and dropped into an inverted microcentrifuge cap containing 50μl buffer RLT (RNeasy Micro Kit - Qiagen) and 1% β-Mercaptoethanol.
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA from COR-LT system cell lines was purified via the DNeasy Blood and Tissue DNA extraction kit according to the manufacturer’s instructions (Qiagen). Unmodified alleles were amplified via primers located outside of the homologous arm sequences ...
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bioRxiv - Microbiology 2023Quote: Total RNA from purified NK cells was isolated using QIAshredder columns and the RNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Infected cells were incubated for 24 hours prior to genomic DNA extraction using the QIAmp DNA Mini Kit (Qiagen). HIV-1 early and late RT products copy numbers were quantitated by real time PCR as described above ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from frozen cells using an RNeasy Plant Mini Kit and DNase treated (QIAGEN, Manchester, UK). First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega ...
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bioRxiv - Bioengineering 2023Quote: Total RNA isolation from cell pellets was performed according to manufacturer’s instructions for RNeasy Mini or Micro kit (cat. 74104 or 74004, Qiagen). RNA was eluted from the columns using 20 µl of RNase-free water and quantified using Nanodrop.
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cells with the RNeasy Mini Kit followed by on-column DNase digest (Qiagen, Cat. #74004). mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown in 6 well plates and total mRNA was isolated using the RNeasy Kit (Qiagen, 74-104) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were harvested in 700 μl Qiazol and total RNA was isolated using the miRNeasy Micro Kit (Qiagen, Netherlands) according to manufacturer’ ss protocol ...
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bioRxiv - Genomics 2023Quote: ... gDNA was extracted from cell lines or buffy coat using the QIAsymphony DSP DNA Midi Kit (cat. 937855, Qiagen) per the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA and RNA were extracted from the B14804-/- and wild-type cells using AllPrep DNA/RNA Mini kit (Qiagen). cDNA was synthesized from extracted RNA using SuperScript™ III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from three independent experiments of the cells or nuclei were prepared using the RNeasy mini-kit (Qiagen) according to the protocol provided by the manufacturer ...
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bioRxiv - Systems Biology 2023Quote: RNA was extracted from cell lysates using the RNeasy Mini Kit and subjected to a DNase treatment according to manufacturer’s protocols (Qiagen) then stored at −80 °C until further use ...
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bioRxiv - Systems Biology 2023Quote: ... toruloides cell pellet was resuspended in 350 μL of Buffer RLT from the RNeasy mini kit (Qiagen, Hilden, Germany). Approximately 500 μL of acid-washed glass beads (acid washed ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we extracted DNA for other strains and purified it using a Blood and Cell Culture DNA mini kit (Qiagen). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted from cell pellets of three biological replicates (i.e., time-separated cultures) using RNeasy Plus Mini Kit (Qiagen) with genomic DNA eliminator columns and resuspended in nuclease-free water according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: RNA was isolated from cultured or isolated cells or snap-frozen brain tissues using the RNeasy Mini Kit (Qiagen) or the RNeasy Lipid Tissue Mini Kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from D0 and D7 3T3-L1 cells using an RNeasy Plus Mini Kit (Qiagen #74134). RNA concentration was measured by NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and its genomic DNA was amplified in the same 0.2-mL PCR tube using the REPLI-g single-cell WGA kit (QIAGEN) to obtain single-worm WGS data from LJ2050 ...
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bioRxiv - Genomics 2023Quote: High-molecular-weight (HMW) DNA was extracted from 30M frozen pelleted cells using the Gentra Puregene extraction kit (Qiagen). Purified gDNA was assessed using fluorometric (Qubit ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the corresponding conditions from 200,000-250,000 cell pellets using the RNeasy Mini Kit (Qiagen #74104) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was extracted from about 1 × 106 cells from all groups using RNeasy Mini Kit (Qiagen, Valencia, CA), then using the random hexamer primer and M-MuLV reverse transcriptase kit (Fermentas ...
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bioRxiv - Bioengineering 2023Quote: ... purified genomic DNA (25 ng) was first amplified according to the manufacturer’s instructions using REPLI-g Single Cell kit (Qiagen). Amplified genomic DNA was then diluted into ∼800 ng/μl and 1 μl DNA was used for each qPCR reaction ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from these cell lysates using the RNeasy UCP Micro Kit protocol and reagents (Qiagen, Cat #73934). DNA was digested via on-column DNase I treatment for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from U2OS and MD-MB-231 cells using the RNeasy Mini Kit (QIAGEN no. 74134). cDNA was synthesized using Superscript III First-strand Synthesis System for RT-PCR kit (Invitrogen ...