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Citations for Qiagen :
3451 - 3500 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from T-ALL cells collected purification using RNeasy Plus Mini or Micro Kit (Qiagen). cDNA were generated using SuperScript® VILOTM cDNA Synthesis Kit (ThermoFisher Scientific).Real-time PCR reactions were carried out using Power SYBR® Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total RNA was extracted from cells using the Qiagen RNeasy Plus Universal Micro Kit following manufacturer’s instructions (Qiagen). Extracted RNA samples were quantified using a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated and RNA was extracted with an RNeasy mini kit (Qiagen 74104), which was then converted to cDNA using Superscript III (Invitrogen 18080051) ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was then harvested for RNA using the RNeasy Plus Mini Kit (#74134, Qiagen, Hilden, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Total RNA from isolated T cells and colon tissue was isolated using the RNeasy Mini Kit (Qiagen, Germany). cDNA was then generated from 200 ng total RNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: High-molecular-mass DNA was extracted from 30Lmillion frozen pelleted cells using the Gentra Puregene extraction kit (Qiagen). Purified gDNA was assessed using fluorometric (Qubit ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were collected from each developmental stage and total RNA was isolated using the RNeasy plus kit (Qiagen), and reverse transcribed with the high-capacity cDNA reverse transcription kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was extracted from these UV-irradiated cells using the QIAamp DNA Blood Mini Kit (51104, Qiagen) and RNase A (19101 ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from 3×106 SYAT or CGR8 cells with the Rneasy Plus Mini Kit (Qiagen, #74134). Reverse transcription was performed on 1 µg RNA using oligodT (Thermo Fisher Scientific #SO131 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1 x 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: RNA extraction from hypothalamus and cultured cells was performed using the miRNeasy Mini Kit (217004, Qiagen, Venlo, Netherlands) according to the instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from cells growing in P24 or P12 wells using the RNeasy plus kit (QIAGEN). The resulting RNA was digested with 2 U of DNAse I for 30 min at 37 °C and further purified using RNA clean & concentrator 5 columns (Zymo research) ...
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bioRxiv - Genomics 2024Quote: Genomic DNA from human CD34+ cells from three normal adult donors was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: RNA from human cell cultures was extracted from 6 well plates using RNeasy Plus micro kit (Qiagen, 74034). The plates were washed once with PBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were lysed in Trizol™ reagent and total RNA was purified using RNeasy Mini kit (Qiagen # 74104). cDNA was generated from isolated RNA at a concentration of 1 μg per 10 μl reaction volume ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted 96h after transfection using homemade cell lysis buffer (10 mM Tris-HCl pH8.5 and 0.05% SDS) or DNeasy Blood & Tissue Kits (Qiagen) to evaluate editing frequencies ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were re-centrifuged and the pellet was used for total RNA extraction using RNeasy kit (Qiagen) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: mRNA analyses were performed by extracting total mRNA from HEPA1-6 cells using RNeasy mini kit (Qiagen, 74104) following manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... and the 6xHis-SUMO tagged proteins were purified from the soluble protein fraction after centrifugation using an Ni2+-NTA Superflow column (Qiagen, Venlo, Netherlands). Next ...
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bioRxiv - Cancer Biology 2021Quote: The recombinant hexahistidine-tagged TNC-C and EDB WT and mutant proteins (at 60 μg of protein / 40 μl beads in PBS) were immobilized to Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at RT for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... His-tagged proteins were purified on NiNTA beads (Qiagen). Purified proteins were eluted with 500 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified with Ni-NTA affinity resin (Qiagen). The aminoacylation assay protocol from Jiongming Lu was then followed (Lu et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Protein were purified using Ni-NTA agrose (Qiagen, 30210) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble protein was mixed with Ni-NTA resin (Qiagen) for 1h at 4 degrees on a nutator ...
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bioRxiv - Biochemistry 2020Quote: ... Fusion proteins were purified through consecutive Ni-NTA (Qiagen), amylose (NEB) ...
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bioRxiv - Biophysics 2021Quote: ... purified N protein was incubated with RNAse A (Qiagen) with 1:15 (RNAse A ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were either purified using NI-NTA agarose (Qiagen) or anti C-tag beads ...
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bioRxiv - Plant Biology 2021Quote: ... labeled proteins were incubated with Ni-NTA agarose (Qiagen) on a rotary shaker for 1 h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified by Ni-NTA agarose (Qiagen) and eluted with lysis buffer containing 300 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: Protein was purified using Ni-NTA agarose column (Qiagen). A detailed protocol is described in supplementary materials and methods.
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bioRxiv - Neuroscience 2020Quote: ... GAPDH protein was purified by Ni-NTA chromatography (Qiagen) under native conditions ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were purified using Ni-NTA resin (Qiagen, Germany) on a gravity flow column followed by size-exclusion chromatography on a Superdex® 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... Protein purification was performed using Ni-NTA resin (Qiagen) equilibrated with the lysis buffer containing 20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were purified by Nibaffinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Genomics 2019Quote: ... We added 200 µl of Protein Precipitation Solution (Qiagen) and centrifuged at 15,000 rpm for 3 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Genes for protein purification were cloned into pQE30 (QIAGEN). Raw 264.7 and U937 cells were cultured in RPIM1640 medium in the presence of 10% FBS ...
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bioRxiv - Genomics 2020Quote: ... total proteins and RNA were extracted (RNeasy Qiagen 74104) according to the manufacturer’s instructions and analyzed.
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bioRxiv - Biochemistry 2024Quote: ... protein purification was performed using Co+NTA agarose (Qiagen), followed by a Phenyl Sepharose column ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified with Ni-NTA Agarose (Qiagen), dialyzed to PBS containing 6 M urea ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were purified with Ni-column chromatography (Qiagen) by following the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were purified through consecutive Ni-NTA (Qiagen), amylose (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was purified through Ni-NTA agarose beads (Qiagen) (Lysis Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Expressed proteins were captured on Ni-NTA Superflow (Qiagen) equilibrated with Buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... protein was captured using Ni-NTA affinity chromatography (Qiagen). Protein was further purified using ion exchange chromatography preceding size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were purified by Ni-NTA affinity chromatography (QIAGEN) and gel filtration chromatography (HiLoad 26/60 Superdex 75 pg ...
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bioRxiv - Immunology 2024Quote: ... Protein content was quantified using a BCA test (Qiagen). Legendplex assay was done as per the manufacturer’s instructions using a X20 Special Order 5-laser Fortessa (BD ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were incubated with Ni-NTA Agarose (Qiagen 30230) resin ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA from melanoma cells and freshly frozen tumors was extracted with the DNeasy purification kit (Qiagen Inc., Valencia, CA). High melanin content was removed with OneStep™ PCR Inhibitor Removal Kit (Zymo Research Corporation ...