Labshake search
Citations for Qiagen :
3901 - 3950 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 140 μl of supernatants from infected cells were subjected to RNA extraction using the QIAamp Viral RNA Mini Kit (Qiagen), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: Cells were harvested and high molecular weight DNA was extracted using the MagAttract HMW DNA Kit (Qiagen N.V., Venlo, Netherlands). Size selection was performed to remove fragments <10 kilobases (kb ...
-
bioRxiv - Cell Biology 2021Quote: ... For PCR amplification of the V5 tag cDNA was extracted from cells and a PCR was performed using the QIAquick PCR purification kit (QIAGEN) and the following primer sequences ...
-
bioRxiv - Microbiology 2019Quote: ... the beads were suspended in Buffer D2 and subjected to multiple displacement amplification using a REPLI-g Single Cell Kit (QIAGEN).
-
bioRxiv - Neuroscience 2021Quote: ... Bacteria were plated on a large 24.5 × 24.5 cm2 plate containing solid media and ampicillin and cells were scraped from the plate for plasmid DNA extraction using a plasmid midi kit (Qiagen) the next day ...
-
bioRxiv - Microbiology 2021Quote: ... the beads were suspended in Buffer D2 and subjected to multiple displacement amplification (MDA) using a REPLI-g Single Cell Kit (QIAGEN).
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested 48 hours after transfection and total RNA was collected using the RNeasy Plus Mini Kit (QIAGEN, #74136). Sample concentrations were measured using a NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ES2 human clear cell carcinoma cells present (wild type) or absent (siRNA) ARID1A were collected and extracted using an RNeasy Plus Micro Kit (Qiagen). Barcoded TruSeq RNA v2 libraries (Illumina ...
-
bioRxiv - Biochemistry 2021Quote: ... The PNPLA2 transcript levels in ARPE19 cells determined by quantitative RT-PCR were normalized using the QuantiTect SYBR Green PCR Kit (Qiagen) in the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: A cell pellet corresponding to 20-25 OD600 units was lysed with glass beads and RNA was isolated from the cell lysate using the RNeasy Mini Kit and RNase-free DNase Set (both Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... and total RNA from the cells was extracted by phase separation with chloroform and subsequently using the RNeasy Mini Kit (Qiagen). RNA-Seq libraries were prepared using TruSeq RNA library Prep Kit v2 (Illumina ...
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from the sorted cell populations using the Qiagen RNeasy® Plus Micro Kit (Qiagen, Catalogue no.- 74034) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... from the iNgn2-H1 cell line was extracted from hPSCs with the DNeasy Blood and Tissue kit according to manufacturer’s instructions (Qiagen, 69504). PCR of the gDNA was performed with the primer pair forward 5’-AGGAAATGGGGGTGTGTCAC-3’ (in the AAVS1 locus ...
-
bioRxiv - Developmental Biology 2022Quote: Extraction of RNA from flash frozen cell pellets or primary tissue was carried out with the miRNeasy kit (Qiagen, 217004). The work surface ...
-
bioRxiv - Microbiology 2022Quote: L cell monolayers were disrupted by scraping and pelleted at 200 x g for 5 min before RNA was isolated from 2 x 105 cells using the RNeasy Plus Mini RNA extraction kit (Qiagen) per manufacturer’s protocol.
-
bioRxiv - Immunology 2022Quote: ... The next day cells were washed off the plates and plasmids were purified using the HiSpeed Plasmid Maxi kit (Qiagen). The resulting replicate libraries were packaged into a lentiviral library using the packaging vectors psPAX2 and pMD2G and the LV-MAX lentiviral production system (ThermoFisher) ...
-
bioRxiv - Genomics 2022Quote: We extracted genomic DNA materials from clonally expanded cells and matched peripheral blood and colorectal tumour tissue using DNeasy Blood and Tissue kits (Qiagen) or Allprep DNA/RNA kits (Qiagen ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA from sorted CD45-EpCam+UEA1+ and CD45-EpCam+UEA1-thymic stromal cells from 4-week-old mice were isolated using the RNEasy kit (Qiagen). cDNA was synthesized from the total RNA and used as a template for real-time relative quantitation for Foxn1 using commercially available probes and reagents (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... Viral RNA was extracted from 140 μL of cell-free culture media using QIAamp Viral RNA mini kit (Qiagen, Germany), according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: SUZ12 isogenic M3 MPNST cells were treated with or without DNMT inhibitors (50 nM daily Decitabine or 4 μM single dose of GSK862) for 4 days followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Bisulfite sequencing was conducted by the IGO core facility at MSKCC ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Plasmid from the initial and final time point was extracted and purified from pelleted cells using a QIAprep Spin Miniprep Kit (Qiagen).
-
bioRxiv - Microbiology 2022Quote: ... for Illumina sequencing most samples were subjected to whole-genome amplification (WGA) with the REPLI-g Single Cell Kit (Qiagen), either directly from few ciliate host cells (four samples ...
-
bioRxiv - Neuroscience 2019Quote: ... reaction was conducted after pressure ejection of the cytoplasm into a microcentrifuge tube containing the REPLI-g WTA single cell kit (Qiagen). Each cell was incubated in a total volume of 5.5 μl at 24°C for 5 min and then cooled to 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: Individual single Giardia cysts or pools of 10 cysts isolated by FACS were subjected to MDA with the REPLI-g single cell kit (Qiagen). MDA was performed according to the manual ...
-
bioRxiv - Cell Biology 2019Quote: ... The upstream and downstream genomic fragments were generated by PCR amplification reactions from the genomic DNA extracted from SUM159 cells using the QiaAmp DNA mini kit (Qiagen). The open reading frame encoding EGFP together with a flexible linker (5’-GGAGGTTCTGGTGGTTCTGGTGGTTCC-3’ ...
-
bioRxiv - Microbiology 2019Quote: ... Total RNA was extracted from all six cell pellets according to the previously described protocol (78) and cleaned with the RNeasy Mini Kit (Qiagen). All RNA samples were then treated with Turbo DNA-free DNase (Ambion ...
-
bioRxiv - Genomics 2019Quote: ... Dovetail staff extracted high molecular weight (HMW) DNA from tissues using the Blood and Cell Culture Midi Kit (Qiagen, GmbH) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: ... DNA and RNA are extracted simultaneously from a single frozen tissue or cell pellet sample using the AllPrep DNA/RNA kit (Qiagen). For FFPE tumor tissues ...
-
bioRxiv - Zoology 2020Quote: ... Up to 100mg of thorax-tissue was obtained from frozen specimens and prepared for DNA-extraction using the Blood & Cell Culture DNA Midi Kit (#13343) by Qiagen, using 100/G Genomic-tips ...
-
bioRxiv - Genomics 2019Quote: ... RNA was extracted from whole-cell blood using the PAXgene™ Blood RNA System Kit (Qiagen, UK. Catalogue No. 762174), following the manufacturer’s protocol for control samples and probands ...
-
bioRxiv - Microbiology 2019Quote: ... Approximately 5×106 HEK293 cells were used to isolate RNA with the All Prep RNA/DNA Mini Kit (Qiagen; 80204). cDNA was generated using 1μg of RNA with oligo(dT ...
-
bioRxiv - Cell Biology 2019Quote: ... Eight to ten hundred adherent DLD-1 cells were washed with PBS and lysed directly with RLT buffer from RNAeasy Mini Kit (Qiagen). Extracted RNA was analyzed for 260/280 and RIN value on Bioanalyzer 2100 (Agilent) ...
-
bioRxiv - Immunology 2019Quote: ... genomic DNA purified from CD8 T cells and bisulfite converted using the EpiTect Bisulfite Kit as per the manufacturer’s instructions (Qiagen, Inc.). Bisulfite-converted DNA was PCR amplified and cloned with the TOPO TA cloning kit (Life Technologies) ...
-
bioRxiv - Synthetic Biology 2020Quote: All cell cultures were lysed with 600uL of buffer RLT Plus from the Qiagen RNEasy Plus Mini Kit (Qiagen, 74136), and were pipetted up and down vigorously to homogenize ...
-
bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
-
bioRxiv - Developmental Biology 2020Quote: ... The plasmid was transformed into chemically competent DH5a cells and purified from individual colony liquid cultures with the Qiagen Spin Miniprep Kit (Qiagen). To make the sgRNA we linearized purified pDR274 containing the guide sequence with Dra1 and used a T7 RNA polymerase for in vitro transcription (NEB) ...
-
bioRxiv - Systems Biology 2020Quote: ... Subsequently cells were harvested and lysed and the genomic DNA was extracted using the DNeasy® Blood & Tissue Kit (Qiagen), according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... and the remaining library preparation steps were performed by following the protocol of QIAseq FX single cell RNA library kit (Qiagen).
-
bioRxiv - Developmental Biology 2020Quote: ... mpeg1-GFP-positive cells from the skin were collected in Qiazol and RNA was extracted using the miRNeasy Micro Kit (Qiagen). Analyses were performed using the FlowJo software (Treestar).
-
bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA was extracted from samples of 2×109 cells after lysis using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNA from cultured cells was isolated with Direct-zol RNA miniprep (Zymogen) and from tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s protocols ...
-
bioRxiv - Pathology 2021Quote: Total RNA from either snap-frozen muscles or cultured cells was isolated using an RNeasy Micro Kit (Qiagen, Hilden, Germany). The mRNA was reverse-transcribed into cDNA with PrimeScript RT Master Mix (TaKaRa ...
-
bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Microbiology 2019Quote: ... Genomic DNA from non-sorted (exponentially growing or stationary) cells was also extracted using Blood and Tissue DNA extraction kit (Qiagen), following the manufacture instructions.
-
bioRxiv - Microbiology 2020Quote: ... pentatrifurcatum 38,39 cells in culture dishes (50 Petri dish cultures) using MagAttract high-molecular-weight (HMW) DNA kit (Qiagen, MD), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Genomic DNA from the cell pellets was extracted and purified using the Qiagen DNeasy Blood and Tissue Kit (Qiagen; 69506) following the Gram-negative bacteria protocol ...
-
bioRxiv - Microbiology 2020Quote: Cell-associated (CA)-RNA and DNA were isolated from cryopreserved peripheral blood mononuclear cells (PBMCs) using the AllPrep DNA/RNA Mini Kit (Qiagen). Unspliced CA-RNA and total HIV DNA levels were quantified using a real-time PCR approach with primers/probes targeting conserved regions of HIV LTR/gag as previously described.8,92 The CA-RNA assay measures levels of unspliced transcripts ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from three cell pellets per condition (prepared as independent biological samples) using the DNeasy UltraClean Microbial Kit (Qiagen) according to the manufacturer’s instructions ...