Labshake search
Citations for Qiagen :
3801 - 3850 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... coli K12 MG1655 genomic DNA was extracted from a cell culture using the DNEasy Blood and Tissue kit (69504, Qiagen). All the other gDNA from the bacteria presented in Table 1 were isolated using the Monarch genomic DNA purification kit (T3010S ...
-
bioRxiv - Genomics 2020Quote: Total RNA was collected from untreated (T0) and 12h R5020- and E2-treated Ishikawa cells using RNeasy Plus Mini Kit (QIAGEN) and subjected to high-throughput sequencing in Illumina HiSeq 2000 and 2500 ...
-
bioRxiv - Genomics 2021Quote: ... The supernatant was then used for high molecular weight (HMW) genomic DNA extraction according to the Genomic-tips protocol (100/G, Blood & Cell Culture DNA Midi Kit, QIAGEN).
-
bioRxiv - Microbiology 2020Quote: ... We sampled 1 ml of the clear phase for living planktonic cells and extracted DNA using DNeasy Blood & Tissue Kit (Qiagen). The cultures were also plated on SHIEH-agar plates with dilutions for cfu/ml -estimation and colony-PCR.
-
bioRxiv - Biochemistry 2021Quote: ... The RNA was prepared from 5 OD equivalents of stressed and unstressed cells using the RNeasy Plus RNA Isolation Kit (Qiagen). 500 ng RNA of the total isolated RNA were used as a template for the synthesis of cDNA using Oligo(dT ...
-
bioRxiv - Microbiology 2021Quote: ... cells were pelleted by centrifugation and genomic DNA was extracted using the Gentra Puregene Yeast/Bacteria kit (Qiagen, Germantown, MD). The purified DNA was used as template for PCR (psdR primers provided in Supplemental Index Table S2 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Single cells of Desulfovibrio or Methanococcus were sorted into wells of a 96-well plate containing 3 µl of PBS and Buffer D2 from Repli-G single cell kit (Qiagen) by using Influx flow cytometer (BD) ...
-
bioRxiv - Physiology 2021Quote: ... 0.5 to 3 million cells were re-suspended in 500 uL TRIzol Reagent and total RNA was extracted using the miRNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... or trl RNA was was calculated using the 2-ΔΔCt method56 using RNA extracted from 500ul of cells following the 6 day incubation using an RNeasy Plus RNA extraction kit (Qiagen). Gene targets were amplified from cDNA using previously validated primers for clamp ...
-
bioRxiv - Genomics 2020Quote: Total RNA was isolated from tissues and cells using RNeasy micro kits with in-column DNase treatments (Qiagen, Toronto, Canada). For cardiac left ventricular samples (n = 7 for each mouse strain) ...
-
bioRxiv - Genomics 2019Quote: Total RNAs from the knockdown (shCTCF#1 and shCTCF#2) and control (shLuc) cells were extracted using miRNeasy Micro Kit (QIAGEN). cDNA was synthesized by using oligo (dT)20 and SuperScript III Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... DNA was extracted from circulating PD-1+ and PD-1− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing.
-
bioRxiv - Immunology 2021Quote: ... DNA was isolated from sorted IFNγ+ and IFNγ− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing ...
-
bioRxiv - Molecular Biology 2021Quote: ... HMG20A cDNA sequence was obtained by PCR from cDNA generated from RNA extracted from HeLa cells using the RNeasy Mini kit (Qiagen) and ProtoScript II First Strand cDNA Synthesis Kit (NEB ...
-
bioRxiv - Molecular Biology 2019Quote: RNA extraction was carried out using the RNeasy Plus Mini Kit Animal Cell Spin technique (Cat# 74106 Qiagen, Mississauga, Canada) using the protocol provided by the manufacturer ...
-
bioRxiv - Immunology 2020Quote: ... in vitro differentiated T-cells were sorted for live cells and lysed with RLT Plus buffer and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen). Full-length RNA-seq libraries were prepared as previously described [47] and paired-end sequenced (75 bp × 2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2 million S2 cells or Kc cells after RNAi treatment were resuspended in Trizol and RNA was purified using the RNeasy Mini Kit (QIAGEN). Afterwards ...
-
bioRxiv - Microbiology 2019Quote: ... DNA was purified from 500µl of this cell suspension using the MO BIO Microbial Ultraclean DNA Purification Kit (Qiagen, Germany). PCR amplifications were done using primers 27F (5’ AGAGTTTGATCMTGGCTCAG 3’ ...
-
bioRxiv - Immunology 2019Quote: ... Cells were transferred to RNase-free microcentrifuge tubes and RNA was isolated using the RNeasy mini kit (Qiagen, cat. #: 74104) including a DNase step (Qiagen ...
-
bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from FACS purified Zeb1+ (Lineage−Sca-1+CD49fhi tdTomato+) or Zeb1− basal cells (Lineage−Sca-1+CD49fhitdTomato−) using a RNeasy micro kit (Qiagen). cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) ...
-
bioRxiv - Cancer Biology 2020Quote: ... a final pool of 20 million cells were harvested for genomic DNA extraction using the QIAamp DNA Blood Maxi Kit (QIAGEN). PCR and sequencing were carried out as described above.
-
bioRxiv - Systems Biology 2020Quote: ... At N24 and N32 Rex1GFPd2 expression was determined by flowcytometry (see Flowcytometry Analysis) and cells were harvested in RLT buffer and isolated with the RNeasy mini kit (Qiagen) according to the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2019Quote: ... The expression construct was transformed into DH10MultiBacY cells and recombinant bacmid was purified with a QIAprep Spin Miniprep Kit (Qiagen). V0 and V1 virus generation was performed using SF9 cells as previously described 44 ...
-
bioRxiv - Plant Biology 2019Quote: ... RNA for ISO-seq was extracted from cells grown for 3 days in mating inducing media (Sørensen et al., 2014) using the RNeasy Mini kit (Qiagen, USA). Nuclei were isolated from the cell pellets (Raimundo et al. ...
-
bioRxiv - Cancer Biology 2019Quote: ... was isolated from human PanNET specimens or cells grown on 6-cm or 10-cm plates using RNeasy mini kit (Qiagen) containing gDNA eliminator spin columns ...
-
bioRxiv - Immunology 2019Quote: ... total RNA (2.5-5 μg) was extracted from BM mononuclear cells obtained by Ficoll-Paque gradient centrifugation using the miRNeasy kit (Qiagen) and depleted of ribosomal-RNA (Ribo-Zero™ rRNA Removal Kit ...
-
bioRxiv - Microbiology 2019Quote: ... Total environmental DNA was isolated from this 100 μL cell suspension by the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol.
-
bioRxiv - Genetics 2020Quote: ... Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR) of levels of Lucia gene DNA present in cell extracts recovered using Qiagen PCR cleanup kits (Qiagen). Lucia luciferase activity was normalised against the crossing point (Cp ...
-
bioRxiv - Genomics 2019Quote: Cells were sorted in an AstriosEQ (Beckmann Coulter) cell sorter and collected in 500 uL of RTL buffer from the RNAeasy extraction kit (QIAGEN). Forward and side scatter (FSC & SSC ...
-
bioRxiv - Plant Biology 2019Quote: ... three replicates of 25 to 30 pooled egg cells were used for total RNA extraction according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (Qiagen). In brief ...
-
bioRxiv - Microbiology 2019Quote: Differentiated PC12 cells infected with PRV were harvested and subjected to total RNA extraction using an RNeasy Plus Mini Kit (Qiagen). First strand cDNA synthesis of isolated RNAs was performed with SuperScript III First Strand synthesis kit with Oligo (dT ...
-
bioRxiv - Neuroscience 2020Quote: RNA was isolated from dissociated DRG neurons or cell body/axon compartments collected from insert cultures using RNeasy micro isolation kit (Qiagen). Purified RNAs were quantified with Ribogreen (Invitrogen ...
-
bioRxiv - Developmental Biology 2020Quote: ... ∼100 ng of total RNA from each cell type was used as input to generate miRNA sequencing libraries using a QIAseq miRNA library kit (Qiagen). Single-end 100 bp reads (1×100 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The remaining leukocytes and circulating tumour cells were washed once with PBS and gDNA was extracted from the pellet using the QIAmp DNA mini kit (Qiagen). Total RNA was isolated from cells after 48h in culture ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from mammalian cell culture after 48 h of pulsatile light activation using RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: DNA was extracted from flow cytometry-sorted T cells using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: RNA isolation from sorted ex vivo NK cells was extracted using the RNeasy Plus mini Kit (#74134, QIAGEN, Hilden, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: Total cellular DNA was isolated from growing or differentiated CIN612-9E cells using the DNeasy Blood and Tissue Kit (Qiagen) at times indicated in the figures ...
-
bioRxiv - Cancer Biology 2020Quote: 106 cells from both FUJIOKA and MOLM14 cells lines were collected 24h post transfection and RNA was extracted using RNeasy Mini kit (QIAGEN), and first-strand cDNA synthesis was performed using standard protocols ...
-
bioRxiv - Cell Biology 2021Quote: ... Mitochondria were purified from approximately 2*106 cultured HeLa cells continuously expressing the mitochondrial matrix marker su9-mCherry using the Qproteome Mitochondria Isolation Kit (Qiagen) following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: Driver mutations were derived from.15 CopyNumbers were calculated using the CNVKit package.16 RNA was isolated from cell cultures (+/− 5 μM AGI-5198) using the RNeasy kit (Qiagen). We performed paired-end sequencing of 2×100 with the Illumina Novaseq platform to obtain 8-10 GB per sample ...
-
P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: Cells were washed twice with ice-cold PBS and total RNA extracted using the RNeasy Extractions Kits (Qiagen, Hilden, Germany) as per manufacturer’s protocol and the quality checked using the Agilent 2200 TapeStation (Agilent Technologies ...
-
bioRxiv - Biochemistry 2020Quote: Total RNA was isolated from cells using the Qiagen’s RNeasy kit (# 74104) according to the manufacturer’s instructions (Qiagen, Germantown, MD). Total RNA (1 ug ...
-
bioRxiv - Bioengineering 2021Quote: ... Tumor areas were micro-dissected to enrich the analyzed specimen with cancer cells and DNA was extracted using the QIAamp DNA Micro Kit (Qiagen). DNA concentration was determined spectrophotometrically by the absorbance measurement at 260nm and all samples were kept at −20oC until use.
-
bioRxiv - Neuroscience 2020Quote: RNA was isolated from dissociated DRG neurons or cell body/axon compartments collected from insert cultures using RNeasy Microisolation kit (Qiagen). Sciatic nerve was cut in small pieces and digested with collagenase at 37°C for 30 min with intermittent trituration ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were then treated with IMiD compounds as indicated before RNA extractions were performed using the RNeasy mini kit (Qiagen). RNA was quantified using a NanoDrop 3300 Fluorospectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated in triplicate from D458 cells treated with 10nM THZ1 or DMSO using RNeasy Mini kit (Qiagen). RNA-seq was performed at Genomics and Microarray Core Facility ...
-
bioRxiv - Cell Biology 2021Quote: RNA samples of MS-5 cells cultured alone and in co-culture with SKM-1 cells extracted using Trizol were purified using the Rneasy Plus Micro kit (Qiagen) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from cells collected from 10 mL late-exponential phase culture in Todd-Hewitt broth with the DNeasy Blood & Tissue kit (Qiagen) using a modified protocol with an additional lysis step as described previously 61 ...
-
bioRxiv - Microbiology 2020Quote: ... Media was refreshed 4 and 8dpi and surviving cells were collected 12dpi for genomic DNA extraction using QIAamp DNA Blood Maxi Kit (Qiagen). gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems ...