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Citations for Qiagen :
3001 - 3050 of 3116 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: DNA was extracted from fecal pellets (100 mg, n=5 per group) by the QIAmp Power Fecal DNA kit (12850-50, Qiagen). The V4 region of 16S rRNA gene was amplified ...
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bioRxiv - Immunology 2023Quote: ... MRC-5/hTERT or A549 cells were reverse transfected with ON-TARGETplus siRNA SMARTpools (Horizon Discovery) or FlexiTube siRNA (Qiagen) using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Aliquots of ∼500,000 isolated B cells were pelleted at 400g for 5 minutes and then resuspended in 350uL RLT Plus buffer (Qiagen). Total RNA was then isolated using RNeasy Mini Plus kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... we FACS-sorted single nuclei in 384-well plates prefilled with 5 μL of Vapor-Lock (Qiagen, cat. no. 981611) per well ...
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bioRxiv - Cancer Biology 2023Quote: ... of 5 million AMO1 shIRE1 cells previously treated per condition was isolated using the AllPrep DNA/RNA Mini Kit (Qiagen). gDNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA Extraction and Purification: Genomic DNA was extracted from 5 M cells using the Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from Day 11 populations (n=20, 5/treatment) using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germany) adapted from the manufacturer’s instructions to include a lysostaphin lysis step(50) ...
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bioRxiv - Immunology 2024Quote: Samples were double-sorted using a FACSAria or FACS Astrios: 600-1000 cells from each population were collected into 5 μl TCL buffer (Qiagen) containing 1% beta-mercaptoethanol (β-ME ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was thoroughly aspirated and replaced with 20 µl of 1x PBS containing 5 mg/ml of RNase A (Qiagen) and 12 mM of purified H6-mNeonGreen-NLS protein as a loading control ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from 5×106 sorted WSU-FSCCL IgG+ and IgM+ cells using the RNeasy mini kit (Qiagen, 74004). RNA quality control was performed using a Bioanalyzer (Agilent) ...
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bioRxiv - Genomics 2024Quote: Total RNA was isolated from 5 × 106 cells harvested from frozen patient vials using the RNeasy Plus mini-Kit (Qiagen). Libraries were prepared with the NEBNext® rRNA Depletion Kit and were sequenced using the Illumina NextSeq500 platform ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from the anterior half of the body from 10-12 5 dpf larvae per sample at using the Rneasy Mini Kit (Qiagen). cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... the anterior segment was pelleted by centrifugation for 5 minutes at 600g and then subjected to total RNA extraction using a RNeasy Mini Kit (QIAGEN). Following RNA extraction ...
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bioRxiv - Genomics 2024Quote: ... Each sample was mixed with 5 µL of one of 6 Illumina adapter sequences (specified in the Qiagen protocol appendix) and 2 µL of ultralow input ligase ...
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bioRxiv - Systems Biology 2024Quote: ... for 5 minutes and immediately lysed in the β-Mercaptoethanol-containing lysis solution from the RNeasy minikit (Qiagen Cat # 74104). RNA was extracted following the Qiagen kit instruction manual and eluted in 10mM Tris-HCl ...
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bioRxiv - Physiology 2024Quote: Tissues were disrupted using stainless steel beads of 5 mm diameter in a Tissue Lyser LT (Qiagen, Venlo, The Netherlands). The resulting tissue homogenates were purified using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µl of serum was centrifuged at 2000 x g for 5 min to remove debris and processed using the DNeasy Blood and Tissue Kit (Qiagen), with the ethanol precipitation step performed overnight at -20°C ...
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bioRxiv - Genomics 2024Quote: ... The pooled cells were centrifuged at 500 × g for 5 minutes at 4°C and resuspended in 100 μl of buffer EB (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... SF was transfected with 12.5 nM antisense LNA GapmeRs CBP (Qiagen, Sequence: 5′-GCG GCG ATC CTT TAG A-3′) or p300 (Qiagen ...
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bioRxiv - Immunology 2024Quote: Parasite DNA quantification by qPCR was conducted on genomic DNA extracted from 5% of every brain homogenate using the DNEasy Blood & Tissue Kit (QIAGEN). As previously outlined (58) ...
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bioRxiv - Genomics 2024Quote: ... RNA was first amplified using a first-round PCR (RdRp S1 5ߣ-GGKTGGGAYTAYCCKAARTG -3’, RdRp R1 5’-TGYTGTSWRCARAAYTCRTG-3’) with the One-Step RT-PCR Enzyme MixKit (Qiagen), targeting a total expected size of 620 base pairs (bp) ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from 5 IGH+ and 7 IGHUND lymphoma cases using either QIAamp DNA FFPE Tissue Kit (QIAGEN) (FFPE tissues ...
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bioRxiv - Zoology 2024Quote: ... Multiplex PCR was performed in 10 µl reaction volume containing 5 µl 2x QIAGEN Multiplex PCR Master Mix (QIAGEN, Germany), 2 µl 5x Q-Solution (QIAGEN) ...
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bioRxiv - Cancer Biology 2024Quote: ... and homogenised in NP40 lysis buffer (as described previously) using mechanical shearing with 5 mm stainless steel metal beads (Qiagen). Debris were cleared by centrifugation ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Multiplex amplification was carried out in 10 μl reaction volumes containing 5 μl of QIAGEN Multiplex PCR Buffer Mix (QIAGEN Inc.), 0.2 μM labeled forward primer (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... flies were individually crushed in 400 µL of lysis buffer by a sterile 5-mm stainless bead shacked by a TissueLyser® (Qiagen) for 30 s at 25 Hz ...
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bioRxiv - Neuroscience 2020Quote: ... RAGE-/- and SOD1G93A x RAGE-/- mice (n = 5 - 6) using RNeasy Lipid Tissue extraction kit according to manufacturer’s instructions (QIAGEN, CA, USA). The total RNA was purified from genomic DNA contamination using Turbo DNase treatment (Ambion ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of plasmid DNA was transfected into each well using Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427). Unless otherwise noted ...
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bioRxiv - Molecular Biology 2021Quote: Cells were seeded in 6-well and 12-well plates at a density of 1.25×105-5×105 cells per well and transfected with miR-320a mimic (Qiagen, 339173 YM00471432) or antagomir (GenePharma ...
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bioRxiv - Immunology 2021Quote: ... Single cells were sorted into individual wells of a 96-well plate containing 5 μl of lysis buffer (TCL buffer (Qiagen #1031576) with 1% of 2-b-mercaptoethanol) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected by centrifugation (5 min at 500 x g) and RNA was isolated using RNeasy Mini Kit (RNeasy Mini Kit, Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and the tissue was homogenized with a 5 mm tungsten carbide bead in a bead mill homogenizer (Qiagen, Tissue Lyser II) at 30 Hz for 1.5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sample was divided into 5 parts and each part was purified using one PCR purification columns (PCR purification kit, Qiagen 28106). 50ul elution buffer was used the elute each column ...
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bioRxiv - Microbiology 2022Quote: ... and the amplified DNA bands from the 5’ and 3’ ends were individually excised and purified with QIAquick® Gel Extraction Kit (QIAGEN). Purified PCR products were cloned into pJET1.2/blunt plasmid (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were centrifuged for 5 minutes at 300g and resuspended in 1mL PBS + RNase Inhibitors (Invitrogen AM2694, Qiagen Beverly Inc Y9240L) and fixed as previously described for split-pool ligation-based RNAseq ...
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bioRxiv - Biochemistry 2020Quote: ... embryo pellets were homogenized by cryo-milling after addition of a 2.5 or 5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 15 seconds 3-6 times ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Genetics 2022Quote: ... 0.08M KOH was added to each well and incubated at room temperature (RT) for 5 min and then washed with EB (Qiagen, 19086). Next ...
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bioRxiv - Genomics 2022Quote: ... The swabs were placed in Red 1.5 mL RINO® screw-cap tubes (NextAdvance) pre-filled with RNase-free zirconium oxide beads and QIAzol Lysis Reagent (Qiagen) was added ...
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bioRxiv - Microbiology 2021Quote: ... 5) 240 µL of the supernatant is subjected to viral RNA extraction using the QIAamp Viral RNA Mini Kit (Qiagen, #52904), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... We checked ASOs penetration in cells by means of the 5′-FAM-labeled control ASO A provided by Qiagen (Figure S5f).
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bioRxiv - Neuroscience 2021Quote: ... 5 ng of each PCR product was pooled into one tube for purification using the QIAquick PCR purification kit (Qiagen #28106) and eluted into 30 µL Nuclease Free Water ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 SCID mT3-2D subcutaneous tumors and the mT3-2D cell line using a DNeasy Blood and Tissue Kit (Qiagen, #69504). Indexed ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from the colon tissue of mice (n=5) by using the RNeasy mini kit (Cat# 74104, Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD14- CD16- CD20+CD38+ BG505 SOSIP+YU2-gp140+ B cells were sorted into individual wells of a 96-well plates containing 5 μl of a lysis buffer (Qiagen, 1031576) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Microbiology 2022Quote: ... The cells were then centrifuged at 300g for 5 minutes and lysed using buffer RLT provided in the RNAeasy Plus Micro Kit (Qiagen; 74034). After the lysate was homogenized using the QIAshredder Kit (Qiagen ...