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Citations for Qiagen :
2951 - 3000 of 3116 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Total RNA (5×106 cells) was extracted from FEA or CRFK cell lines by using the RNeasy Plus Mini Kit (Qiagen). RNA from FEA ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA for PIWIL3 5′ RACE was extracted from the ovaries of 8-week-old golden hamsters using ISOGEN (Nippon Gene) and RNeasy (Qiagen). 5′ RACE was performed using the SMARTer RACE 5/3 kit (Takara Bio ...
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bioRxiv - Immunology 2021Quote: ... Cells were rinsed at rested at 37°C for a minimum of 1h before undergoing red-blood-cell lysis by 5-10 ml RBC lysis solution (Qiagen) for 20 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... and 3-week-old) and leaves (4-, 5-, and 6-week-old plants) was isolated and purified using RNeasy Plant Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Remaining cell cultures were spun down at 4000 rpm for 5 min and the cell pellet resuspended in 50 μL nuclease-free water (Qiagen). Total RNA was extracted using a hot acidic phenol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The eluted RNA from tissues and swabs was analyzed by one-step qRT-PCR using 5 μL input RNA with the QuantiFast Probe PCR kit (Qiagen) and primer/probe sets targeting the NiV Malaysia and HeV N gene ...
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bioRxiv - Developmental Biology 2022Quote: ... medial and proximal samples for each replicate were homogenised with an OMNI TH handheld 5 mm tip probe in 400 μl lysis buffer (Qiagen) for 20 seconds ...
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bioRxiv - Developmental Biology 2022Quote: A repair template containing TagRFP-T::AID with homology at the 5’ and 3’ ends to the egl-43 locus was PCR amplified and purified using a PCR purification kit (Qiagen). 3 μl of 10 μM tracRNA (IDT ...
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bioRxiv - Genomics 2022Quote: ... were annealed (10 μL each 100 μM) to 10 μL 5′-[Phos]-CTGTCTCTTATACACATCT-3’ oligonucleotide (100 μM) within 80 μL EB buffer (Qiagen), incubating 2 min at 95°C and cooled to room temperature (0.1°C/sec) ...
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bioRxiv - Microbiology 2022Quote: ... the slurry was diluted to an OD600 of 5 and genomic DNA was purified using the DNeasy Blood & Tissue DNA purification kit (QIAGEN). In parallel ...
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bioRxiv - Immunology 2022Quote: Cells were centrifuged for 5 minutes at maximum speed and RNA was extracted with the RNeasy Plus Micro Kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: Cyanobacterial cells were collected by centrifugation of liquid cultures at 10000 g for 5 min and DNA was extracted from cell pellets with the DNeasy PowerBiofilm kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: mRNA was isolated from Boyden chamber migration assay-derived Treg pellets from 5 HD and 4 uRRMS patients using RNeasy micro kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was sonicated for 5 min and genomic DNA was extracted using the QIAamp DNA Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was sonicated for 5 min and nuclear gDNA was extracted following the protocol of the DNeasy Blood & Tissue kit (Qiagen) with addition of RNase A treatment.
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 3-5 mm ear primordia of the inbred line B73 using RNeasy Mini Kit (Qiagen) with on-column DNase I (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... For each experimental condition total RNA was prepared from 5 OD600 units of cells using the RNeasy Plus RNA Isolation Kit (Qiagen). The synthesis of cDNA was performed using 500 ng of prepared RNA ...
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bioRxiv - Biophysics 2022Quote: ... Cell debris was cleared by centrifugation at 40,000 g for 45 min at 4°C and supernatant was applied to His-tag affinity purification using Ni-NTA agarose (beads or 5 ml column, Qiagen) in buffer A (50 mM TRIS ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 5 million cells per cell line using the Qiagen AllPrep DNA/RNA extraction kits (Qiagen, 80004). Nucleic acid quantification and quality detection were performed and RNA sequencing libraries were generated via Illumina Stranded mRNA prep ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from 5-20 mg of melanoma primary and metastatic tissues was extracted using AllPrep DNA/RNA Mini isolation kit (Qiagen). A total of 100 ng of total RNA was used as input for RNA- seq libraries using SMARTer Stranded Total RNA-seq - Pico input (Takara Bio USA ...
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bioRxiv - Synthetic Biology 2023Quote: The pARP3 plasmid for methylation analysis was extracted from 200 mL of an overnight culture of Tol 5 transformant cells using the HiSpeed Plasmid Midi kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was clarified by centrifugation at 5 °C at 18500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB3_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 5 °C at 18 500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB2_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Developmental Biology 2024Quote: Pools of 5 embryos were used for RNA extraction via the Qiagen RNeasy Micro Kit (Qiagen, Valencia CA; Cat# 74004). RNA was eluted in RNase-free water and sent to the New York University Genome Technology Center for RNA-sequencing ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Microbiology 2023Quote: ... These tissues were then weighed and homogenized by either Omni homogenizer (Omni International, Omni Tissue Homogenizer (TH) 115V) or by 5-mm stainless steel beads with a TissueLyser (Qiagen) and used for onward analysis of infectious virus and vRNA quantification ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated for 30 min at 4 °C with 5 mL of Ni2+-beads (Qiagen Ni-NTA Superflow) equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... DNA for metagenomes was extracted from 5 and 0.2 μm filters (47 mm diameter) using the DNAeasy Power Soil Pro kit (Qiagen, Sweden). DNA from viral fraction (142 mm diameter membranes ...
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bioRxiv - Neuroscience 2023Quote: ... After this step the samples were adjusted to RT for 5 minutes and processed using the RNeasy Lipid Tissue Mini Kit (Qiagen) as described in the user manual ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA extraction from all fractions was performed using Qiazol at 65°C for 5 minutes with regular mixing and then purified with miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and precipitated with ammonium sulfate as in Niu et al.49.The precipitate was dissolved in 30 ml buffer C and loaded onto a 5 ml column of Ni-NTA-agarose (Qiagen) pre-equilibrated in buffer C ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 100 µL DTT (100 mM) at RT for 5 min and recovered by the RNeasy clean up kit (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was resolved by 1.5 % agarose followed by excision and isolation within the size range of 300-600 bp using Gel Extraction Kit (Qiagen). NGS libraries were pooled and quantified by qPCR using NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed and incubated in Proteinase K (Roth) and Ribonuclease A (Roth; 5 min, RT) before isolating cellular and viral DNA with DNeasy Blood & Tissue Kit (Qiagen). qPCR was performed using FastStart Universal Probe Master (Roche ...
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bioRxiv - Genomics 2023Quote: ... Second strand synthesis was performed after thawing by the addition of 5 µL of second strand synthesis mix (3 µL of elution buffer [Qiagen] ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from pooled triplicates 5 hpi with RNeasy Mini columns according to the manufacturers instructions (RNeasy Kits, Qiagen) and strand-specific qPCRs were performed as described previously (38) ...
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bioRxiv - Cancer Biology 2023Quote: ... The material stored in Qiazol was heated at 65°C for 5 min and RNA was extracted using the RNeasy Lipid Tissue Kit including the optional DNase digestion (Qiagen). RNA quality and concentration were assessed using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
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bioRxiv - Genomics 2022Quote: ... We incubated the reaction for 5 minutes at 55 °C in a G-Storm GS1 (G-Storm) thermal cycler and after incubation added 5 μl Buffer PB (19066, QIAGEN) to inactivate and strip the transposase from the tagmented DNA ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... The sample was stored with RNAprotect following the manufacturer protocol (Protocol 5 from RNAprotect Bacteria Reagent Handbook 01/2020, Qiagen) at -80°C until processed ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and added to appropriate volumes of buffer RLT and homogenised at 50 Hz for 5 minutes using the TissueLyser LT (Qiagen), until all tissue was homogenised ...
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bioRxiv - Immunology 2023Quote: ... 5×104 cells were resuspended in buffer RLT supplemented with ý-mercaptoethanol and processed with an RNeasy Micro Kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with either the Pin-point system or SpCas9 was extracted 5 days post electroporation using the Gentra Puregene Cell Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 1µM Template-Switching Oligo (5′-AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3′, where “r” prefixes a ribonucleic acid base and “+” prefixes a locked nucleic acid base, Qiagen) then incubated using a ThermoMixer C with the following conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 5 mM DTT) and incubated for 45 minutes at 65°C before addition of 40 μg Proteinase K (Qiagen) and further incubation for 1.5 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Then this amplified product was labeled with biotin at 5’ end followed by purification using the Qiaquick nucleotide removal kit (QIAGEN).To check the drug-protein interaction ...