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Citations for Qiagen :
2801 - 2850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 1.5×106 cells using the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was added to a 500 μl equilibrated Glutathione Sepharose 4 Fast Flow affinity medium (GE) or Ni-NTA agarose (Qiagen). The lysate was incubated with an affinity matrix for 1 hour at 4 ⁰C ...
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bioRxiv - Molecular Biology 2024Quote: ... and Actin primers were obtained from Quantitect (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: ... on ice for 30 min using a TissueLyser II (Qiagen, Venlo, Netherlands). After centrifugation at 16,000 × g for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 400 ng purified PCR product (PCR purification kit, Qiagen) was denatured by incubating at 95°C for 5 min and the temperature was slowly lowered to < 30°C to allow the formation of heteroduplex ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mg tissue was processed using the Blood and Tissue DNeasy kit (QIAGEN). 100 ng DNA was used for TaqManTM PCR quantification with transgene-specific probe/primer sets normalized to the TERT gene (Supplementary Table 2).
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated using an RNeasy kit (Qiagen) and reverse transcription was performed with the Quantitect kit (QIAGEN) ...
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bioRxiv - Neuroscience 2024Quote: ... and mRNA was purified using Oligotex beads (QIAGEN). mRNA was treated with EZ-DNase (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... and reverse transcription was performed with Quantitect kit (QIAGEN). Spliced transcripts were quantified by TaqManTM PCR using GloX4 primer-probe set specific for transgene exon-exon junction (Table 2) ...
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bioRxiv - Neuroscience 2024Quote: ... and reverse transcription was performed with the Quantitect kit (QIAGEN). Library cDNA was subjected to PCR for Illumina NGS sequencing as described below in the in vivo screening methods.
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from 100–200 mg of tissue using RNeasy plus universal kit (QIAGEN), and reverse transcription was performed with Quantitect kit (QIAGEN) ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from 1-5E5 sorted cells using the DNeasy blood and tissue kit (Qiagen, 69504) and used for barcode amplification and NGS analysis.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5-mm stainless steel beads (Qiagen) at 25 Hz for 1.5-3 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction was performed using a combination protocol between the NucleoSpin® Plant II kit from Macherey-Nagel and the DNeasy plant mini kit from QIAGEN. Approximately 2 g of each lichen sample was grinding and crushed with a mortar and pestle for distribution of the sample and convert the lichen samples into powder ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA samples were extracted from peripheral blood leukocytes of each subject using a QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from two batches of 50 adult females using the PureGene Core Kit A (Qiagen) and the two gDNA extracts were then pooled in equal proportion into a single sequencing library ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purified on RNeasy mini columns (Qiagen) and QC checked using Qubit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA samples were then treated with RNase-free DNase set (Qiagen) to remove gDNA contamination ...
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bioRxiv - Pathology 2024Quote: ... Genomic DNA was isolated from EDTA anticoagulated blood according to the membrane-based QIAamp DNA extraction protocol (Qiagen, Hilden, Germany). The common genetic variants involved known to modulate the risk of fatty liver ...
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bioRxiv - Pathology 2024Quote: ... 20µL Proteinase K on a TissueLyser II (Qiagen) for 2 min at 25 Hz and 3 min at 30 Hz ...
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bioRxiv - Pathology 2024Quote: RNA from cells was isolated using the RNeasy Mini Kit or RNeasy Micro Kit (74104 and 74004, respectively, QIAgen, Germany), following manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... including on-column DNA digestion (RNase-Free DNase Set, 79254, QIAGEN). RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 500 uL of the homogenate was processed using the RNeasy Plant Mini kit (Qiagen) according to manufacturer’s protocol (centrifugations steps were performed at 12,000 rpm for 30 seconds) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was then isolated from the samples using a RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... CcAP1 and CcFULLike-A and CcFULLike-B with CLC Genomics Workbench v21.0.3 (QIAGEN, Aarhus, Denmark) (Supplementary Table S5) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the supernatant was removed and purified using a DNeasy Blood & Tissue Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... were harvested at the bolting stage using liquid nitrogen and ground to powder form using tissue lyser (Tissuelyser II, Retsch, Qiagen) at 30 frequency/ s for 2 mins and lyophilised overnight ...
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bioRxiv - Bioengineering 2024Quote: Datasets were imported to Ingenuity Pathway Analysis (IPA, Qiagen) for calculating pathway activation patterns or z-scores.
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bioRxiv - Plant Biology 2024Quote: ... 65°C treatment with EDTA and cleaned with the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). Depletion of ribosomal RNA (rRNA ...
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bioRxiv - Bioengineering 2024Quote: ... EVs were then isolated by the miRCURY Exosome Isolation Kit (Qiagen, #76743) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... root samples or 3rd and 4th leaf samples were separately harvested and ∼500 mg of samples were used for total RNA extraction using RNeasy Plant Mini Kit (supplied by Qiagen, Cat #74136). One µg of total RNA was used to obtain cDNA using Reverse Transcription Super-mix (supplied by Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated from whole seeds as described in the RNeasy Plant Mini Kit (Qiagen). Sequencing libraries were prepared with TruSeqTM RNA library prep kit (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... 100⍰mg of ground powder from each tissue was used for RNA isolation using the RNeasy Plant Mini Kit (#74904, QIAGEN Ltd UK) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Clarified cell lysates were purified using Ni-NTA resin (Qiagen) to produce highly purified and active enzyme ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
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bioRxiv - Plant Biology 2024Quote: The total RNA was isolated from 50-100 mg of cells using RNeasy Plant Mini kit (Qiagen, Düsseldorf, Germany) and treated with DNA-Free kit (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR products were purified with QIAquick PCR & Gel Cleanup Kit (Qiagen, Hilden, Germany). All amplicons were directly Sanger-sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Physiology 2024Quote: ... centrifuged and washed with M9 for three times and subjected to RNA extraction using TissueDisruptor and the RNeasy Mini Kit from Qiagen. Three biological replicates were included for WT and APOE4 ...
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bioRxiv - Physiology 2024Quote: Total RNA was isolated using RNeasy Minikit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: 50 mg of mouse spleens were homogenized in 500 μl of the same lysis buffer using the TissueLyser LT (Qiagen, #85600) (50 sec for 2 min ...
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bioRxiv - Physiology 2024Quote: ... 5plex Platform system (QIAGEN). The reaction was run in the QIAcuity Nanoplate 26k 24-well plates (QIAGEN) ...
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bioRxiv - Physiology 2024Quote: All chicks were genetically sexed from DNA extracted from erythrocytes (Qiagen DNeasy Blood and Tissue kits ...
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bioRxiv - Physiology 2024Quote: ... The 40 µl reaction mix consisted of 10 µl of 4x QIAcuity Probe PCR Kit (QIAGEN), 2 µl of the target assay ...
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bioRxiv - Physiology 2024Quote: ... Absolute quantification of Myc and Rpl3 expression was carried out using the QIAcuity Software Suite (QIAGEN).
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bioRxiv - Physiology 2024Quote: GAS muscles were pulverized on a metal block cooled with liquid nitrogen before lysis and RNA extraction using the RNeasy Fibrous Tissue Mini Kit (74704, Qiagen). RNA purity and integrity was examined with a Bioanalyser (Agilent).
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bioRxiv - Physiology 2024Quote: ... and NR4A3-overexpressing (NR-203Oex) plasmid DNA was then purified using endotoxin-free buffers (Cat. No. 19048) and columns (Cat. No. 10083) from Qiagen, before assessing DNA concentration and purity by spectrophotometry.
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bioRxiv - Physiology 2024Quote: ... The reaction was run in the QIAcuity Nanoplate 26k 24-well plates (QIAGEN). The 40 µl reaction mix consisted of 10 µl of 4x QIAcuity Probe PCR Kit (QIAGEN) ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing reads were processed by the CLC Genomics Workbench 9.5.1 software (Qiagen). Reads were mapped to the Aethionema arabicum PacBio contigs originating from the CYP accession 13 ...
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bioRxiv - Physiology 2024Quote: ... lysed in 300µL of RLT buffer (Qiagen) and stored at -80°C until extraction of all samples ...
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bioRxiv - Physiology 2024Quote: ... DNeasy Blood & Tissue Kits were used (QIAGEN #69506). After extraction ...