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Citations for Qiagen :
2201 - 2250 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 1μg of total RNA was reverse transcribed using the miScript II RT Kit (Qiagen). For qRT-PCR analysis of miRNAs ...
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bioRxiv - Genetics 2020Quote: ... mRNA reverse transcription (RT) is performed with QuantiTect reverse transcription kit (Qiagen, Hilden, Germany).
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bioRxiv - Genomics 2020Quote: ... The cDNA of WCR midgut was synthesized by Omniscript RT kit (Qiagen, Germantown, MD). To validate the pattern of AS ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was detected using One-step probe RT-qPCR kits (Qiagen) run on the CFX96 detection system (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was detected using One-step probe RT-qPCR kits (Qiagen) run on the CFX96 detection system (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg total RNA using the QuantiTect RT kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesised from 500 ng RNA using the miScript II RT kit (Qiagen) with HiFlex buffer ...
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bioRxiv - Genomics 2020Quote: ... and cDNA was synthesized using the miScript II RT Kit (Qiagen, 218160, California, USA) following the manufacturer’s instructions and stored at −80°C until use ...
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bioRxiv - Immunology 2021Quote: ... The RT-qPCR RNA was isolated from three cultures using RNeasy Mini kit (Qiagen), the cDNA was synthesized using SensiFAST reverse transcriptase (Bioline) ...
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bioRxiv - Cell Biology 2021Quote: ... 250 or 500 ng of total RNA using the miScript II RT kit (Qiagen) and the cDNA was amplified with miScript SYBR Green PCR (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Reverse transcription was carried out using the miRCURY LNA™1 RT Kit (Qiagen) using 10 ng of RNA according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cDNA was synthesized from 500ng of total RNA using the Omniscript RT-Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: RNA was reverse transcribed to cDNA using Sensiscript RT Kit reagents (Qiagen, cat # 205213) with RiboGuard RNase inhibitor (Lucigen ...
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bioRxiv - Cell Biology 2023Quote: RNA samples for RT-qPCR analysis were generated by using RNeasy Mini kits (Qiagen) using ∼2 million cells as starting material ...
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bioRxiv - Microbiology 2023Quote: ... RNA was reverse transcribed into cDNA using the Qiagen QuantiTect RT kit (Qiagen, 205311), including negative controls ...
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bioRxiv - Bioengineering 2023Quote: ... complementary DNA (cDNA) was synthesized using miRCURY LNA RT Kit (Catalog No. 339340, Qiagen). For each miRNA target ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was generated from total RNA using the miRCURY LNA RT Kit (QIAGEN, 339340) and then amplified in duplicate by qPCR using the miRCURY LNA SYBR Green PCR Kit (QIAGEN ...
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bioRxiv - Developmental Biology 2023Quote: ... The extracted RNA was retro-transcribed using the miScript II RT kit (QIagen, 218161). qPCR for miR quantification was performed with miScript SYBR Green PCR kit (QIagen ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription of 1 μg RNA was performed using Omniscript RT kit (Qiagen, #205111). CLN3 transcript was amplified ...
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bioRxiv - Cell Biology 2021Quote: ... following manufacturer’s instructions and gene-specific primers on a Corbett Rotor-gene qPCR machine (Qiagen). Relative quantification was obtained with the ΔΔct method using Hprt or 29s as internal control ...
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bioRxiv - Cell Biology 2022Quote: ... and random primers from RNA extracted and prepared using the RNeasymini kit (Qiagen, CA, USA) following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2019Quote: ... or Mm_Prkaca_1_SG and Mm_Tbp_1_SG QuantiTect Primer Assay with SYBR Green for Prkaca and Tbp respectively (QIAGEN). Relative expression values were calculated using the ΔΔCt method including normalization with the Tbp values.
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bioRxiv - Genetics 2021Quote: ... using custom or vector primers and plasmid DNA purified with the QIAprep Spin kit (Qiagen). Reads were assembled using MacVector software.
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bioRxiv - Developmental Biology 2022Quote: ... Primers for GAPDH (Geneglobe ID: QT00079247) and ADRB2 (Geneglobe ID: QT00200011) were purchased from Qiagen and primers for mEGFP-ADRB2 were self-made (refer to “Oligonucleotides”).
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bioRxiv - Developmental Biology 2021Quote: ... The PCR products were column-purified using the QIAquick PCR Purification Kit (Qiagen, 28106) and subjected to in vitro transcription using the MEGAscript T7 Transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Multiplex microsatellite PCR amplifications were performed with a multiplex PCR kit (Qiagen Inc.®) for 26 microsatellite markers as previously described (Cornille et al. ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... The PCR product was transcribed with QIAquick PCR Purification Kit (#28104, Qiagen, Hilden, Germany), transcribed with MAXIscript Kit (#AM1320 ...
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bioRxiv - Immunology 2019Quote: ... and qRT-PCR was performed using the rotor-gene SYBR green PCR kit (Qiagen) (see Supplemental Table 1 for gene names and sequences used) ...
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bioRxiv - Plant Biology 2020Quote: ... For qRT-PCR was performed using the SYBR® Green PCR kit (Qiagen, 204054). Plant GAPDH was used as the internal reference to normalize the cDNA content ...
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bioRxiv - Microbiology 2019Quote: ... The PCR reaction was cleaned and concentrated using the QIAquick PCR Purification Kit (QIAGEN), eluting in 30 μl H2O ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany) before the IVT reactions.
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bioRxiv - Microbiology 2019Quote: PCR products were purified using the Nucleospin Gel and PCR kit (QIAGEN, Hilden, Germany). The resulting amplicon was sequenced using the Sanger method by the College of Biological Sciences UCDNA Sequencing Facility (http://dnaseq.ucdavis.edu/) ...
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bioRxiv - Genetics 2019Quote: ... PCRs were carried out with the QIAGEN multiplex PCR kit (Qiagen GmbH, Hilden, Germany) following the instructions of the manufacturer in three multiplexes combining different product sizes and fluorescent dyes ...
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bioRxiv - Genetics 2019Quote: ... These PCR products were purified using a QIAquick PCR Purification Kit (QIAGEN, Hilden, Germany), then 1 μg of each was used as template for synthesis of dsRNA using a MEGAscript™ T7 Transcription Kit (ThermoFisher) ...
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bioRxiv - Genetics 2019Quote: ... PCR products were purified using the QIAquick PCR purification kit (Qiagen, Montreal, Quebec, Canada).
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were purified using a QIAquick PCR Purification Kit (Qiagen, cat no: 28104) and were then cloned using a pGEM→-T Easy Vector System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified by a QIAquick PCR Purification kit (Qiagen, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... real-time quantitative PCR was performed using the miScript SYBR Green PCR kit (Qiagen) on ABI Prism 7900HT (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... the PCR product was purified using the Qiaquick PCR Purification Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Thirty-five microliters of PCR products were purified using QIAquick PCR Purification kit (QIAGEN) and sequenced to confirm site-specific integration and successful insertion of Nsi1 (primers in Supplementary Table 3) ...
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bioRxiv - Microbiology 2020Quote: qRT-PCRs were performed using the QuantiNova SYBR Green PCR Kit (QIAGEN, Venlo, Netherlands). 1 μl cDNA was added to 19 μl master mix (containing 10 μl SYBR Green ...
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bioRxiv - Immunology 2020Quote: ... PCR-amplified DNA barcodes were purified using the QIAquick PCR Purification kit (Qiagen, 28104) and sequenced at PrimBio (USA ...
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bioRxiv - Microbiology 2021Quote: ... Each PCR contained 25 µL of 1 × Taq PCR Mastermix (Qiagen GmbH, Hilden, Germany), final concentration of 0.2 µM of each primer ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of template DNA and the Qiagen PCR multiplex PCR kit (Qiagen, France) on a T100TM Thermal cycler (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplified and then DNA was isolated using the Qiagen PCR purification kit (Qiagen). Assembly of the final donor plasmid was performed by Gibson Assembly (New England BioLabs) ...
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bioRxiv - Biophysics 2020Quote: ... The resulting PCR amplification products were purified using the Qiaquick PCR purification Kit (Qiagen). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... The first PCR was performed with the PCR Multiplex Plus Kit (Qiagen, Hilden, Germany) using 12.5 μl master mix ...
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bioRxiv - Microbiology 2022Quote: ... PCR arrays were performed according to the PCR array handbook from the manufacturer (Qiagen). Real-time PCR was performed using RT2 Profiler PCR array in combination with RT2 SYBR green master mix (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... These PCR products were purified using the QIAquick PCR purification kits (QIAGEN cat.no. 28104) and then subjected to T7-Endonuclease 1 (T7E1 ...
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bioRxiv - Microbiology 2021Quote: ... and the remaining PCR product was purified with the MinElute PCR Purification Kit (Qiagen).