Labshake search
Citations for Qiagen :
2001 - 2050 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products were isolated by QIAquick PCR Purification Kit (Qiagen; 28104) and subjected to Sanger sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were cleaned using the MinElute PCR purification kit (Qiagen 28004) and the selection process was repeated four times with PCR products from prior rounds used as the starting library ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using a QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified using the MinElute PCR Purification kit (Qiagen). Amplicons were cloned into the LentiGuide-Puro vector (digested according to the lentiviral CRISPR toolbox protocol developed by Zhang lab(37 ...
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bioRxiv - Microbiology 2024Quote: ... and PCR products were purified before cloning (QIAquick PCR purification kit, QIAGEN). Amplicons were cloned in front of the luxCDABE operon in the plasmid pUC18R6KT-mini-Tn7-KmR-luxCDABE into the SpeI and XmaI restriction sites (highfidelity enzymes ...
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bioRxiv - Immunology 2024Quote: ... PCR products were purified using the QIAquick PCR purification kit (#28106; QIAGEN). Sanger sequencing was performed on an ABI 3730 XL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... PCR Buffer 10x (Qiagen), 0.2 mM dNTPs and 400 mM primers ...
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bioRxiv - Cell Biology 2024Quote: ... PCR Buffer 10x (Qiagen), 0.2 mM dNTPs and 400 mM primers ...
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bioRxiv - Plant Biology 2023Quote: ... PCR purification kit (QIAGEN) was used for DNA purification after proteinase K treatment for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... 1X PCR buffer (Qiagen), 0.5 mM of each primer ...
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bioRxiv - Developmental Biology 2020Quote: cDNAs were synthesized from total RNA (corneal) using the Omniscript RT kit (Qiagen) with oligo-dT (dT20 ...
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bioRxiv - Neuroscience 2021Quote: ... 500 ng of total RNA was transcribed using the QuantiTect RT Kit (Qiagen). Real-time PCR was performed with 1 µl cDNA per reaction using the Power SYBR Green PCR Master Mix (ThermoFischer Scientific ...
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bioRxiv - Immunology 2019Quote: ... and 1µg of total RNA was reverse transcribed with Omniscript RT kit (Qiagen) according to the manufacturerś instructions ...
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bioRxiv - Genetics 2020Quote: ... cDNA was retrotranscribed from total RNA extracted using the Omniscript RT kit (Qiagen). For mitochondrial transcripts CoI and Nd4 ...
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bioRxiv - Microbiology 2021Quote: ... For reverse transcription and synthesis of complementary DNA the Omniscript RT Kit (Qiagen) using random hexamers (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using the Exiqon miRCURY LNA Universal RT microRNA kit (Qiagen). qPCR for individual miR-17∼92 members was performed using miRCURY LNA probes and miRCURY LNA SYBR Green PCR Kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: cDNA synthesis was carried out using the miRCURY LNA RT Kit (Qiagen #339340) from human purified EVs or plasma RNA (10 ng ...
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bioRxiv - Bioengineering 2021Quote: ... The cDNA was added to RT² SYBR Green ROX qPCR Mastermix (Qiagen 330520) and the solution was added to a Custom RT2 PCR Array 384-well plate and qPCR program was run using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems 4485701) ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was reverse transcribed using Omniscipt TM RT kit (Qiagen, US. Catalog# 205113) and the complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis for miRNA was done with the miRCURY LNA RT kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: RNA conversion to cDNA was performed using the miRCURY LNA RT Kit (Qiagen, Cat No ...
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bioRxiv - Neuroscience 2020Quote: ... and each sample was reverse transcribed using miScript II RT Kit (Qiagen, #218161). qRT-PCR reactions were performed with miScript SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: The RT-qPCR was performed on site using a RotorGene 5plex platform (Qiagen). Primers and Taqman dual labelled probes targeting Mtb and the internal control were procured from Eurofin Genomics ...
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bioRxiv - Immunology 2020Quote: ... The RNA was reverse transcribed to cDNA using the miScript RT kit (Qiagen). miRNAs were quantified using miScript Primer Assays (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... mRNA reverse transcription (RT) was performed using the QuantiTect reverse transcription kit (Qiagen) in accordance with the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... 18 µl of Omniscript enzyme at 4 U/µl (Omniscript RT Kit, Qiagen), and 37 µl water ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA was then synthesized with an Omniscript Reverse Transcription (RT) Kit (QIAGEN). Quantitative PCR reactions were carried out in a CFX Connect Real-time PCR detection system (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was prepared with reverse transcriptase using an Omniscript RT kit (205113, Qiagen) following the standard protocol provided ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was completed using QuantiNovaTM RT kit (Qiagen-Hilden, Germany: Ref#205413) according to manufacturer’s guidelines ...
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bioRxiv - Genetics 2021Quote: Dissected larval or adult guts were homogenised in 150 μL of QIAzol Lysis Reagent (QIAGEN, 79306), and stored at −80 °C ...
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bioRxiv - Genetics 2021Quote: Pools of 150 flies per sample were homogenized using a TissueLyser (Qiagen, Inc., Germantown, MD, USA). Genomic DNA was extracted with the Gentra Puregene Tissue kit (Qiagen Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining 150 μL of RLT-dissolved embryo were used for RNA extraction (QIAGEN RNeasy Kit), the RNA was quantified and equal amounts were used for reverse transcription ...
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bioRxiv - Plant Biology 2020Quote: ... Paired sequence reads of 150 bp were aligned using CLC Genomics Workbench 10.0.2 (QIAGEN, Aarhus, Denmark). The Arabidopsis genome assembly of ftp.ensemblgenomes.org/pub/current/plants/fasta/arabidopsis_thaliana/dna/Arabidopsis_thaliana.TAIR10.dna.toplevel.fa.gz (downloaded 10.8.2019 ...
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bioRxiv - Physiology 2022Quote: ... approximately 150-200 mg of snap frozen placenta tissue was lysed in RLT-lysis buffer (Qiagen) with homogenization aided by a tissue-lyzer ...
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bioRxiv - Microbiology 2024Quote: ... 150 μl of the supernatant was extracted with a DNeasy blood & tissue kit (Qiagen, Maryland, USA), and were extracted as purified DNA in 50 μl of distilled water ...
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bioRxiv - Physiology 2024Quote: Approximately 150–200 mg of placenta tissue was lysed and homogenized in RLT-lysis buffer (Qiagen) using a tissue homogenizer ...
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bioRxiv - Molecular Biology 2021Quote: ... the PCR products were held at 4°C until PCR purification using the QIAquick PCR purification kit (#28106, Qiagen, Hilden, Germany). DNA concentrations were determined using NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... and real-time reverse transcription quantitative PCR (qRT-PCR) was performed using QuantiNova™ SYBR Green PCR Kit (Qiagen, Hilden, Germany). Primers were synthesized by IDT Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... The primers were designed by use of the Pyromark Assay Design Software package (Qiagen), and 10 CpG sites of the FOXP3 intron region were selected for quantitative DNA methylation analysis ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed with specific primers for mouse IFN-α11 (PPM03050B-200, Qiagen), mouse β-actin (PPM02945B-200 ...
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bioRxiv - Neuroscience 2021Quote: ... These genes were compared against the housekeeping gene HPRT1 (Cat No. QT00199640, Qiagen Primer) and relative gene expression was quantified using the 2^ddCT method ...
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bioRxiv - Cancer Biology 2020Quote: ... All data were normalized to the housekeeping gene 18S (18S QuantiTect Primer Assay; Qiagen).
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bioRxiv - Immunology 2020Quote: Random-primer reverse transcription was done using RT2 First Strand kits (Qiagen; Valencia, CA), including a genomic DNA removal treatment ...
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bioRxiv - Cancer Biology 2022Quote: ... TBP and GAPDH were proprietary primers that were designed by Qiagen (Qiagen, Hilden, Germany). The samples were then subjected to quantitative Real-Time PCR (RT-qPCR ...
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bioRxiv - Genetics 2022Quote: ... Sequencing was performed using the sequencing primer TGTAGGATGAAGTCTTACA and PyroMark Gold Q24 Reagents (Qiagen) according to the manufacturer’s instructions on a PyroMark Q24 pyrosequencer (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... IL −6 and TNFα mRNA were detected by validated QuantiTect primer assays 144 (Qiagen).
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bioRxiv - Microbiology 2024Quote: Mouse cDNA was amplified with gene-specific primers and HotStarTaq Master Mix Kit (Qiagen) (Table 1) ...
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bioRxiv - Genetics 2023Quote: ... with biotin-labeled methylation-specific primers designed with Pyromark Assay Design Software 2.0 (Qiagen). The primer sequences are shown in Supplemental Table S1 ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... including DNase I digestion via the RNase-Free DNase Set to remove traces of genomic DNA (Qiagen, Hilden, Germany). Quality and quantity of the isolated RNA was examined with the Fragment Analyzer (Advanced Analytical Technologies GmbH ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...