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Citations for Qiagen :
2051 - 2100 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... followed by additional purification using RNeasy spin columns with DNase treatment according to the manufacturer’s instructions (Rneasy Mini Kit; RNAse-Free DNase Set; QIAGEN). RNA was reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was further purified by an on-column DNAse digestion using the RNase-Free DNase Set (Qiagen, Cat# 79254) and two rounds of washes using the RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... Each dataset was mapped to the to the predicted gene sets (NCBI Annotation Release 100) using CLC Genomics (Qiagen). Reads were mapped with the following parameters ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA was subjected to on-column DNase digestion with the RNase-free DNase set (Qiagen, Cat. No. 79254), cDNA synthesis and RT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... and an on-column DNase I digest was performed with the Qiagen RNase-Free DNase Set (Qiagen, Hilden, Germany). RNA was eluted in 50 μl of RNase-free molecular biology grade water and DNA was removed by incubation with 1 μl TURBO DNase (2 U/μl ...
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bioRxiv - Immunology 2021Quote: ... lysed with Qiazol lysis reagent and total RNA was purified using miRNeasy kit and RNAse-free DNAse set according to the manufacturer’s instructions (Qiagen). RNA quality was checked using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions including an on-column DNaseI digestion step using the RNase-free DNase Set (Qiagen). First- strand cDNA synthesis and PCR reaction was performed as previously described.45 Exonspanning primers (Eurofins Genomics ...
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bioRxiv - Systems Biology 2021Quote: ... RNA-isolation for NGS applications was performed according to manufacturer’s protocol (Qiagen RNeasy mini kit, RNase free DNase set). For detection of protein abundance by western blotting ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Genomics 2021Quote: ... according to the manufacturer’s instructions with the addition of an on-column DNase digestion step using the RNase-free DNase Set (QIAGEN). Reverse transcription was performed using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Further RNA extraction was performed according to the manufacturer’s instructions including an on-column DNase digestion using the RNase-Free DNase Set (Qiagen). RNA purity and quantity was measured using the Nanodrop (Nanodrop Technologies ...
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bioRxiv - Genetics 2022Quote: ... DNase digestion and RNA clean-up were performed using RNeasy Mini kit and RNase-Free DNase set (Qiagen, MD), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from bacterial strains using a Qiagen Genomic DNA Buffer Set (Qiagen, Germany; Cat. No. 19060) and Qiagen genomic tip 100/G gravity-flow ...
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bioRxiv - Cancer Biology 2024Quote: ... A DNase treatment of RNA has been added before RNA Cleanup using the RNase-Free DNase set from Qiagen following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Off-column DNase treatment of all inner ear samples was carried out using the RNase-Free DNase Set (Qiagen). Quantity and quality of the obtained total RNA samples were analyzed using a spectrophotometer (Nanodrop ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR reactions were set up in duplicate in the Rotor-Gene Q thermocycler 5 plex (Qiagen, Germantown, MD, USA). Primers reported here were designed using Primer BLAST (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Microbiology 2023Quote: ... was applied and a DNase digestion was carried out by using the RNase-Free DNase Set (Qiagen, Hilden, Germany).
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bioRxiv - Neuroscience 2023Quote: ... Total RNA extraction and DNase treatment were carried out using miRNeasy Micro Kit and RNase-free DNase set according to the manufacturer’s instructions (QIAGEN).
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bioRxiv - Neuroscience 2023Quote: ... following the manufacturer’s instructions including DNase I treatment using RNase-Free DNase Set and on-column protocol (QIAGEN, USA). The isolated total RNAs were aliquoted for both RNA-seq and qRT-PCR.
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bioRxiv - Immunology 2024Quote: ... The RNAse-Free DNAse set was used to digest DNA during RNA purification according to the manufacturer’s instructions (Qiagen). The purity of RNA was assessed by calculating the ratio of OD260/OD280 ...
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bioRxiv - Plant Biology 2024Quote: ... and the reaction mixture and the programme conditions were set according to the supplier’s instructions and run on a Rotor-Gene Q Machine (QIAGEN). UBIQUITIN C (UBC) ...
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bioRxiv - Cell Biology 2021Quote: ... and the PCR product was purified using QIAquick PCR Purification Kit (Qiagen #28106), then shuttled to Gateway™ pDONR™221 Vector (Invitrogen#12536017 ...
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bioRxiv - Immunology 2021Quote: ... the PCR products were purified using the MinElute PCR purification kit (Qiagen, UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR reactions were column-purified using the QIAquick PCR Purification Kit (Qiagen). Sequencing adaptors and barcodes were added with a second round of PCR using the KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Real-Time PCR was performed using miRCURY SYBR Green PCR Kit (Exiqon-Qiagen) according to the manufacturer’s instructions with StepOnePlus Real-Time PCR System (ABI ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were purified with the QIAquick PCR Purification kit (Qiagen; Hilden, Germany), then underwent in vitro transcription using the mMessage Machine T7 Transcription Kit (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... Single and pooled animal genotyping PCR reactions used Taq PCR Master Mix (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... PCR product was purified following the protocol for “QIAquick PCR Purification Kit” (Qiagen). Agarose gel electrophoresis showed right amplicon sizes and single bands ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining PCR product was purified using the QIAquick PCR purification kit (Qiagen) and sent to an external service (AllGenetics ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products were purified using MinElute PCR purification kit (Qiagen, Dusseldorf, Germany). Lentiviral vector LentiCRISPR-v2 was digested with Esp3I (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The remaining PCR reaction was purified using the QIAquick PCR Purification kit (QIAGEN), and the purified DNA product was sequenced at the Dartmouth Molecular Biology Core Facility using the Applied Biosystems 3730 DNA Analyzer ...
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bioRxiv - Pathology 2019Quote: ... The PCR products were cleaned up using QIAquick PCR Purification Kit (QIAGEN Inc.) according to manufacturer’s instructions and then direct sequenced using Sanger sequencing technology in the Genomics Core Facility ...
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bioRxiv - Genetics 2020Quote: ... The resulting PCR products were purified using QIAquick PCR Purification Kit (Qiagen®) and then undergoing Sanger sequencing (Genomics® ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were then purified using the Qiaquick PCR extraction kit (Qiagen), as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The final PCR products were purified with QIAquick PCR Purification Kit (QIAGEN #28106) and quantified with the KAPA Library Quantification Kit (Kapa Biosystems #KK4844) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were purified using the QIAquick® PCR purification kit (QIAGEN Inc.) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting PCR products were purified using a QIAquick PCR Purification Kit (Qiagen) and checked by agarose electrophoresis ...
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bioRxiv - Genetics 2021Quote: ... The PCR reaction was column-purified with the QIAquick PCR Purification Kit (Qiagen). Sequencing adaptors and barcodes were added with a second round of PCR using the KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Genetics 2021Quote: ... PCR products were then pooled and purified using QIAquick PCR purification kits (Qiagen). Different libraries were then quantified with Kappaquant to determine relative concentrations of amplified product ...
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bioRxiv - Genetics 2021Quote: ... The 1st PCR products were purified by MinElute PCR Purification Kit (28004, Qiagen) and eluted in 13 μl Elution Buffer in the kit ...
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bioRxiv - Genetics 2021Quote: ... The 2nd PCR products were purified by MinElute PCR Purification Kit (28004, Qiagen) and eluted in 10 μl Elution Buffer in the kit ...
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bioRxiv - Microbiology 2020Quote: ... and quantitative PCR reactions were performed with the QuantiTect Probe PCR Kit (Qiagen), using 1 ng of extracted phage DNA in 25 μL reaction volume ...
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bioRxiv - Physiology 2020Quote: ... PCR reactions were transferred to a qRT-PCR thermocycler (Rotorgene 3000Q, Qiagen, UK) with supporting software (Hercules ...
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bioRxiv - Plant Biology 2021Quote: Quantitative real-time PCR was performed with QuantiTect SYBR Green PCR Kit (Qiagen) in a CFX96 Real-Time Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were also purified using QIAquick PCR Purification Kit (Qiagen, Germany) and the fragment sizes were evaluated using 2100 Agilent BioAnalyzer (Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... and then the PCR products were purified using QIAquick PCR Purification Kit (Qiagen). DNA concentration was measured with a Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR fragment was purified using the QIAquick PCR purification kit (Qiagen) and transformed into PY79 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were purified using the MinElute PCR Purification Kit (Qiagen, Hilden, Germany). Six serial 10-fold dilutions (101–106 copy numbers/reaction ...
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bioRxiv - Immunology 2020Quote: ... PCR products were column-purified using the QIAquick PCR Purification Kit (Qiagen, #28106). For targeted TCR reconstitution of TnT cells ...
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed using the QuantiTect SYBR Green PCR kit (Qiagen, Germany) on the ABI QuantStudioTM6 Flex system (Roche ...