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Citations for Qiagen :
1851 - 1900 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... An 18S reference gene was amplified using the QuantiTect Primer Assay Kit (Qiagen). The primers were purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Gene expression levels were analyzed using predesigned QuantiTect primer assays (Hs_GAPDH_1_SG: QT00079247 Qiagen, Hs_FPR1_1_SG ...
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bioRxiv - Neuroscience 2023Quote: ... were predesigned and validated by QIAGEN and used diluted to a final work solution of 1x (QuantiTect Primers, QIAGEN). Appropriate negative controls were also prepared ...
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bioRxiv - Microbiology 2023Quote: ... All primers were manually designed in CLC Main Workbench (CLC Bio, Qiagen, Australia) and supplied by Macrogen Ltd ...
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bioRxiv - Plant Biology 2023Quote: ... using specific primers (Supplemental Table 1) in a Rotor-Gene Q machine (Qiagen). Standard curves were achieved by dilutions of one cDNA sample ...
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bioRxiv - Cancer Biology 2023Quote: ... The transcript levels of NOTCH3 were measured using QuantiTect primer assay (QT00003374, Qiagen), the sequences of the other targets are provided in Table 2 ...
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bioRxiv - Microbiology 2019Quote: ... PCR clean-up was performed with UltraClean PCR Clean-Up Kit (MoBio) or UltraClean 96 PCR Cleanup Kit (Qiagen) before quantification of amplicon products with Quant-iT dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real time PCR (qRT-PCR) analyses were performed with the QuantiFast SYBR Green PCR Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol using gene specific primers (Supplementary Table S3) ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR libraries generated from the second PCR were pooled and purified using QIAquick PCR purification kit (Qiagen, Catalog #28106). Samples were diluted to a final concentration of ~2 nM after they were quantified using the Qubit dsDNA HS Assay kit (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... The DNA in each total RNA sample was digested using DNase I (RNase-free DNase sets, Qiagen, CA). The RNA-seq library was prepared through the Lasy-Seq v1.1 protocol (Kamitani et al. ...
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bioRxiv - Genomics 2021Quote: ... RNA was purified following an on-column DNAse digestion using the RNase-Free DNase Set (Qiagen, Venlo, Netherlands) and cleaned up with the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... An on-column DNase treatment was performed using the Qiagen RNase-Free DNase Set (79254; Qiagen Hilden, Germany). The sample was eluted in 30ul of RNase free water and stored at −80°C prior to QC and library preparation ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s protocol and contaminating DNA was removed with the RNase-Free DNase Set (Qiagen, UK).
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bioRxiv - Microbiology 2021Quote: ... An extra step to remove contaminating DNA on-column was included using the RNase-Free DNase Set (Qiagen). The integrity of the RNA and absence of contaminating DNA was checked by agarose gel electrophoresis ...
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bioRxiv - Genomics 2022Quote: ... Between 50 and 75 ug of nucleic acids were treated with 6.8 units of DNase I using manufacturer’s buffers and columns (RNase-free DNase set; Qiagen) and 1g of glycogen ...
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bioRxiv - Microbiology 2022Quote: ... 14 μL of filtered supernatant was combined with 4 μL of RDD buffer (Qiagen, RNase-Free DNase Set) and 2 μL of DNase I and dissolved as per the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... Crushed tissue was immersed in RLT lysis buffer (from RNeasy Mini Kit and DNase Digestion set 217004, Qiagen) and vigorously vortexed ...
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bioRxiv - Biochemistry 2023Quote: ... Screening plates were set up by a sitting-drop vapor diffusion method using JCSG Core I-IV (Qiagen), PEG Suite I-II (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A set of an additional 25 specimens was extracted with the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) at the Hong Kong University of Science and Technology and sent for metagenomic library preparation and NovaSeq6000 2x150 bp sequencing at Novogene ...
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bioRxiv - Plant Biology 2023Quote: ... USA) with an on-column DNase digestion step (RNase-Free DNase Set, Product Code 79254, Qiagen, Maryland, USA). Obtained RNA was quantified using an ND1000 Nanodrop (Thermo Scientific ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was purified using a QIAGEN Genomic-tip 500G and QIAGEN DNA Buffer Set (QIAGEN, Hilden, Germany), following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2024Quote: ... DNase treatment was made by adding 40 µL of DNase solution mix (10μl DNase I Stock solution + 30μl Buffer RDD; RNase-Free DNase Set, Qiagen) to each column ...
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bioRxiv - Synthetic Biology 2024Quote: ... according to the manufacturer’s instructions with in-column DNase treatment with the RNase-Free DNase Set (Qiagen, USA). Total RNA samples were then converted to Cappable-seq libraries according to the published protocol59 available at www.neb.com/en-us/protocols/2018/01/19/cappable-seq-for-prokaryotic-transcription-start-site-determination ...
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bioRxiv - Cell Biology 2021Quote: ... The pre-PCR amplicons were subjected to quantitative real-time PCR analysis using the Rotor-Gene Probe PCR Kit (Qiagen) performed in a Rotor-Gene Q instrument (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... amplified PCR products were run on agarose gel to verify that the PCR worked and the remaining PCR products were purified using a PCR purification kit (Qiagen) before Sanger sequencing using the forward primer ...
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bioRxiv - Molecular Biology 2021Quote: ... The corresponding PEA1 targeting constructs were used as the PCR template and the PCR amplicon was purified by QIAquick PCR Purification Kit (Qiagen). IVT was performed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were used as the template for quantitative reverse transcription-PCR (qRT-PCR) in technical triplicate using the QuantiNova SYBR Green PCR kit (Qiagen). mRNA levels were normalized to the geometric mean of RPL11 ...
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bioRxiv - Microbiology 2022Quote: ... The expected size of PCR products was confirmed by agarose gel electrophoresis and PCR products were purified using QIAquick PCR purification kit (Qiagen). Purified PCR products were sequentially digested with the appropriate restriction endonuclease (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... Both the first and second PCR were checked on gel and the second PCR was purified using the QIAquick PCR Purification kit (Qiagen). Samples were mixed in equal amounts and sequenced on an Illumina NovaSeq 6000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... All PCR products were purified using the MinElute PCR purification kit (QIAGEN) or Monarch PCR & DNA Cleanup Kit (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then qRT-PCR was performed using QuantiTect-SYBR GREEN PCR Kit (Qiagen) in StepOne Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were purified on QIAquick PCR purification columns (Qiagen, Cat #28106) and loaded onto a 20% polyacrylamide/TBE gels ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were purified using the Qiaquick PCR Purification Kit (Qiagen, 28104), and sequenced at the Department of Biochemistry Sequencing Facility (University of Cambridge).
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified using the QIAquick PCR Purification Kit (QIAGEN, #28104) and cloned using the TOPO® TA Cloning (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... The PCR product was purified using the QIAQuick PCR Purification Kit (Qiagen) and eluted on MiniElute columns (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... Around 400ul PCR products were purified using MinElute PCR purification kit (Qiagen) to obtain the dsDNA donor pool which was verified by agarose gel electrophoresis ...
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bioRxiv - Genetics 2019Quote: ... PCR reactions were processed through a PCR clean-up column (Qiagen #28104), and the concentration determined using a QuBit dsDNA HS Assay (ThermoFisher Q32854).
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bioRxiv - Plant Biology 2020Quote: PCR products was gel purified using the PCR purification kit (Qiagen, USA) and restricted using BamHI and Sac1 for directional cloning into pBI121 binary vector (Chen et al ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified with the Qiaquick® PCR Purification Kit (Qiagen) and sequenced with the ABI BigDye terminator chemistry in an ABI 3730 DNA sequencer (Applied Genomic Technologies Core ...
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bioRxiv - Biophysics 2019Quote: ... The PCR product was purified using a QIAquick PCR purification kit (Qiagen). Monovalent streptavidin was produced by the Howarth lab.30 Block copolymers methoxy-poly(ethylene glycol)-block-poly(L-lysine hydrochloride ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were cleaned up using a QIAquick PCR Purification Kit (QIAGEN) and ligated with the NEBuilder® HiFi DNA Assembly Cloning Kit using a 1:2 (mol/mol ...
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bioRxiv - Genomics 2020Quote: ... followed by qRT-PCR with miScript SYBR Green PCR Kit (Qiagen: 218073) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Both PCR products were purified using the QIAquick PCR Purification Kit (Qiagen) and serially diluted 10-fold from 1010 to 104 copies/reaction to form the standard curve.
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bioRxiv - Immunology 2021Quote: ... All PCR products were purified using the QIAquick PCR Purification Kit (Qiagen) and diluted (final amount ...
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bioRxiv - Bioengineering 2021Quote: ... Amplified PCR products were column-purified (QIAquick PCR Purification Kit, QIAGEN, Germany) and their concentration was evaluated by NanoDrop (NanoDrop ...
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bioRxiv - Bioengineering 2019Quote: ... PCR produces were purified using QIAquick PCR Purification Kit (Qiagen Cat. 28106).
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were purified using the QIAquick PCR Purification Kit (Qiagen) and serially diluted 10-fold from 1010 to 104 copies/reaction to form the standard curve.
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bioRxiv - Plant Biology 2020Quote: ... the PCR products were purified with MinElute PCR Purification Kit (QIAGEN, 28006), and then were used for library construction using MGIEasy AmpSeq Library Prep Kit (MGI ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were then purified using the MinElute PCR Purification Kit (Qiagen) according to the manufacturer’s specifications and concentration (ng/ml ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time PCR was performed using QuantiTect SYBR green PCR kit (Qiagen) and the ABI prism 7900HT sequence detection system (Applied Biosystems) ...