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Citations for Qiagen :
1801 - 1850 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: PCR products were purified using the QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR products were purified using MinElute PCR Purification Kit (Qiagen) to 30 μL final volume and quantified using a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... PCR amplicons were then purified using QIAquick PCR Purification kit (Qiagen). Purified PCR amplicons were subjected to Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using a PCR purification kit (Qiagen, 28006) and then cloned into the AbVec expression plasmids to produce recombinant human IgG1 mAbs (a gift from Patrick C ...
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bioRxiv - Neuroscience 2023Quote: ... PCR product was purified using the QIAquick PCR purification kit (Qiagen) followed by A-tailing and ligation into a pGEM®-T Easy vector ...
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bioRxiv - Biochemistry 2022Quote: ... PCR amplicon was purified twice using PCR purification kit (QIAGEN; 28104). Fragment size was confirmed using agarose gel electrophoresis and the DNA concentration was quantified using absorbance at 547 nm ...
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bioRxiv - Biochemistry 2023Quote: ... PCR fragments were purified using a QIAquick PCR purification kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Second-round PCR products were purified with PCR purification columns (Qiagen) and quantified by 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2022Quote: ... PCR amplified library was purified using PCR cleanup columns (Qiagen #28206) and quantified for PE-150bp sequencing (Novogene).
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bioRxiv - Genetics 2023Quote: Amplified DNA was PCR-purified using QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol and prepared for Sanger sequencing using the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: The PCR amplicons were purified using a PCR purification kit (Qiagen). This PCR amplicon was fused with Kozak 3XHA (~100 bps ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was purified using a PCR purification kit (Qiagen) according to the manufacturer’s instructions and quantified with a 2100 Agilent Bioanalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was then purified using PCR Purification Kit (Qiagen). The concentration of DNA was measured using Quant-iT Picogreen Assay (Thermofisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... The PCR products were purified using QIAquick PCR Purification Kit (Qiagen). Then ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were purified using QIAquick PCR Purification Kit (Qiagen, 28104). DNA library preparations ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was PCR-purified using Qiagen PCR purification kit (Qiagen, #28106). The amplified fragments and the pcDNA5/FRT/TO vector were then digested with the enzymes listed in the names of primers in the table above ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were purified using the QIAquick PCR purification kit (Qiagen) according to manufacturer’s protocol and submitted with corresponding sequencing primers for Sanger sequencing to confirm target modifications using the TIDE algorithm ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting PCR products were purified using MinElute PCR Purification Kit (Qiagen) and submitted for Illumina sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the MinElute PCR purification kit (Qiagen). Fragments were digested using EcoRI-HF (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products were then purified using QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol and quantified concentration using NanoDrop ND-1000 device (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Digital PCR was performed using the QIAcuity EvaGreen PCR kit (Qiagen) and the chlamydiae-specific primer pair Chl40F (5’ CRG CGT GGA TGA GGC AT 3’ ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using QIAquick PCR Purification Kit (Qiagen, Germany). Transposomes were prepared using 100 ng transposon DNA and EZ-Tn5 transposase (Lucigen ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were purified using a QIAquick PCR Purification Kit (Qiagen). Unique barcode adapters were annealed to each sample ...
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bioRxiv - Microbiology 2024Quote: ... The resulted PCR amplicons were purified (QIAquick PCR Purification Kit, Qiagen) and ligated into pBAD/Thio-TOPO plasmid vector (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... All the PCR products were purified (MinElute PCR purification Kit, QIAGEN) and digested with NotI (New England BioLabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR-amplified templates were purified (QIAquick PCR purification kit, Qiagen #28106) and verified for the presence of a single DNA band of the expected size on a 1% TAE-agarose gel ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified using QIAquick PCR Purification Kit (Qiagen), and then further amplified for 7 cycles in the second round of PCR containing 40 ng dsDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the MinElute PCR Purification Kit (Qiagen) and in vitro transcribed to produce antisense RNA-probes using the DIG Probe Synthesis Kit (Roche) ...
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bioRxiv - Immunology 2024Quote: ... PCR products were purified with the MinElute PCR Purification Kit (Qiagen). Cloning was performed with the TOPO TA Cloning & Bacterial Transformation kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified with a QIAquick PCR purification kit (Qiagen) and size-selected one last time with an E-Gel SizeSelect II 2% ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified with a QIAquick PCR Purification kit (Qiagen), molarity was assessed with the Agilent 2100 and samples were sequenced on the Illumina NextSeq550.
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bioRxiv - Cancer Biology 2024Quote: ... Second-round PCR products were purified with PCR purification columns (Qiagen) and quantified by 2100 Bioanalyzer (Agilent ...
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bioRxiv - Neuroscience 2022Quote: ... Mice received either miRCURY LNA power inhibitor for mmu-miR-335-5p (Ant-335; sequence: 5’-CATTTTTCGTTATTGCTCTTG-3’; Qiagen, Cat No.: 339132; 0.1 nmol in 2 μL PBS), or a non-targeting scrambled control (Scr ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted in 150 μl phenol-chloroform and purified on a QIAquick column (Qiagen) and eluted in 50 μl EB buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Immunology 2022Quote: ... then 150 µL of homogenate was added to an equal volume of buffer RLT (Qiagen) for RNA extraction ...
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bioRxiv - Physiology 2020Quote: ... RNA from human islets (∼150 for each donor) was extracted with RNeasy Mini Kit (Qiagen) and was reverse transcribed using the High Capacity Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from 150-200 islets using the RNeasy Micro kit (Qiagen, Valencia, CA). RNA was quantified by spectrophotometry with a NanoDrop 2000 (Life Technologies Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments <150 bp in length were removed using the GeneRead Size Selection Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Primers used for expression studies were purchased from Qiagen (QuantiTect Assays, Hilden. Germany): Ccl2 (QT00167832) ...
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bioRxiv - Plant Biology 2020Quote: ... using gene-specific primers (Table S1) by employing a Rotor-Gene Q6000 (Qiagen). qPCR conditions were composed of three steps of cycling ...
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bioRxiv - Microbiology 2021Quote: ... and β2-microglobulin (B2M) mRNAs were performed using validated QuantiTect primer assays (Qiagen). B2M levels were used as an endogenous control for normalization ...
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bioRxiv - Biochemistry 2021Quote: ... GAPDH transcripts were detected by RT2 qPCR Primer Assay (Qiagen, Cat# 330001 PPQ00249A) and the qPCRBIO SyGreen Mix Hi-ROX kit (PCRBIOSYSTEMS) ...
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bioRxiv - Pathology 2022Quote: ... The transgene was detected using RT2 qPCR Primer Assays from Qiagen (Venlo, Netherlands), and as a normalization gene we used (GAPDH or beta-actin) ...
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bioRxiv - Cancer Biology 2020Quote: ... or GAPDH (catalog no. QT00273322) as an endogenous control (Quantitect Primer Assay, Qiagen). Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... GAPDH transcripts were detected by RT2 qPCR Primer Assay (Qiagen, Cat# 330001 PPQ00249A) and the qPCRBIO SyGreen Mix Hi-ROX kit (PCRBIOSYSTEMS) ...
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bioRxiv - Genetics 2019Quote: ... 20 pmol of each primer and 1 U of Taq polymerase (Qiagen, USA) and 1 µl of the DNA template ...
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bioRxiv - Cancer Biology 2020Quote: ... Primer design was carried out using PyroMark Assay Design software (version 2.0, Qiagen). Pyrosequencing of biotinylated PCR products was performed using PyroMark Q48 Advanced CpG reagents (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... using commercially available primers for human FAAH (CpG Island 100530) (EPHS100530-1A, Qiagen). Methylation-sensitive (EPHS115450-1A ...
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bioRxiv - Cancer Biology 2022Quote: ... the respective Quantitect Primer pairs for detection of specific mRNAs (Qiagen, Hilden, Germany), and StepOnePlusTM qPCR system (2-ΔΔCT method) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Primer extension reaction was performed with one unit of Taq polymerase (QIAGEN, Germany), 0.2 mM dNTP mixture ...