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Citations for Qiagen :
1951 - 2000 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... HIS-fused p300 was expressed from pFastBac1 vector in High-Five cells and purified on Ni-NTA agarose beads (Qiagen, 30210) in BC buffer (20 mM HEPES pH7.9 ...
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the RNeasy Fibrous Tissue Mini Kit (Qiagen 74704), according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... posterior eye cups (PECs) were separated and collected in 2 mL reinforced tubes (SPEX Sample Prep) containing 5 mM stainless steel beads (Qiagen, LLC).
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bioRxiv - Microbiology 2024Quote: ... followed by rotation of the adapter and ten additional minutes of bead beating using a TissueLyser II (QIAGEN; Cat. No. 85300) using a 2 ml Tube Holder Set (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... and 10 zirconium beads were added to approximately 5 mg of intestinal tissue stored at -80 °C in RNA later tissue reagent (Qiagen, Germany). A Tissue lyser (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... PI-negative fraction was sorted in each well of a 96 well plate containing 3 μL of PBS (Qiagen, REPLI-g SC Kit) with 0.2% Tween 20 ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos that reached blastocyst stage were lysed as described above and underwent whole-genome amplification using the Repli-G Mini kit (Qiagen, Inc., Valencia, CA). Target regions were amplified using primers developed using Primer3 (Supplementary Information ...
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bioRxiv - Cell Biology 2021Quote: Drosophila S2R+ cells were transfected with 0.5 µg DNA (pAC-Gal4+pUASp-GEM, or pAC-LAMP1-GFP 77) in 12-well plate using Effecetene transfection kit (Qiagen, Cat. # / ID: 301425) and then were plated 48 hours after transfection on concanavalin A-coated glass coverslips 78 ...
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bioRxiv - Genomics 2020Quote: ... The enriched mtDNA was then amplified by multiple displacement technology (MDA) using specific primers (REPLI-g Mitochondrial DNA kit, Qiagen, Cat No. 151023), followed by library construction (Nextera XT Sample Prep Kit ...
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bioRxiv - Bioengineering 2020Quote: Embryos that reached the blastocyst stage were lysed and underwent whole-genome amplification using the Repli-G Mini kit (Qiagen, Inc., Valencia, CA). To determine presumptive off-target sites ...
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bioRxiv - Microbiology 2021Quote: ... Intact bacterial cells were pelleted by centrifugation at 10,000 x g for 5 min at room temp and DNA extracted from the pellet using a DNeasy® Blood & Tissue kit (Qiagen, Hilden, Germany). DNA concentration was assessed by the QubitTM dsDNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Whole genome amplification of single sorted cells was carried out by multiple displacement amplification with the REPLI-g single cell kit (Qiagen, Cat. No. 150345), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fecal material (0.25 g wet weight) using the QIAamp Power Fecal Pro DNA kit (QIAGEN GmbH, Cat. No. 51804) and was quantified using Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We inspected individual embryos under a dissecting scope for evidence of development and then isolated them for whole genome amplification using the REPLI-g Midi kit from Qiagen (catalog number 150043). For each WGA DNA sample ...
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bioRxiv - Genomics 2021Quote: ... Nuclei pellets and single cells were used to perform WGA using REPLI-g Advanced DNA single cell kit (QIAGEN; Cat No./ID: 150363) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplify the remaining circular DNA in the reaction by MDA using φ29 DNA polymerase and random hexamer primers (REPLI-g Mini Kit (Qiagen, Hilden, Germany, 150025)) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Microbiology 2022Quote: DNA from each tube was amplified via multiple displacement amplification (MDA) using the Repli-g single-cell kit (Qiagen, Hilden, Germany, Cat. #150343) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Whole genome amplification (WGA) nuclei pellets were carried out using the REPLI-g Advanced DNA single-cell kit (QIAGEN; Cat No./ID: 150363), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was then purified by mixing with beads at a 1:0.6 DNA/beads ratio followed by 3 washes with 70% ethanol and eluted with 30 μl of elution buffer (Qiagen Cat# 19086). Whole-exome sequencing was performed using the Mouse_Exome_Targets baitset from the Wellcome Sanger Institute pipeline ...
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bioRxiv - Genetics 2020Quote: ... placed in 2ml Eppendorf (Hamburg, Germany) tubes and disrupted with a pre-cooled Stainless-Steel Beads (5 mm) in the TissueLyzer (QIAGEN, Venlo, Netherlands). The genomic DNA (gDNA ...
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bioRxiv - Microbiology 2022Quote: ... Organs were homogenized by high-speed shaking in 2 mL microcentrifuge tubes filled with sterile PBS and 5/7 mm stainless steel beads using TissueLyser LT (Qiagen, Hilden, Germany).
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bioRxiv - Evolutionary Biology 2019Quote: ... bacteria were removed from beads by vortexing in PBS prior to DNA extraction using the Qiagen DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany). For planktonic samples ...
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bioRxiv - Genomics 2020Quote: ... was rinsed in 1X phosphate buffered saline, homogenized with Bullet Blender Bead Lysis Kit (NextAdvance, Troy, NY) and the supernatant passed through QIAshredder columns (Qiagen, Hilden, Germany) before been used for tumor DNA extraction using the Allprep DNA/RNA/miRNA Universal Kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... Lyophilized material was pulverized in a 2ml screw top microcentrifuge tube with a glass bead for 10 minutes at a frequency of 20/s in a TissueLyser (Retsch ballmill, Qiagen, Venlo, Netherlands). The material was AIR prepped and destarched using protocols from Barnes and Anderson (Barnes and Anderson ...
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bioRxiv - Microbiology 2021Quote: ... ethanol-stored flies were rehydrated in water and transferred to 1.5 ml well plates for homogenisation using a bead beater (Qiagen Tissue Lyzer II). Protein was digested using Proteinase K ...
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bioRxiv - Microbiology 2020Quote: ... Tissues were homogenized in 1 ml cell culture medium (see above) and a 5 mm steel bead in a TissueLyser (Qiagen, Hilden, Germany). Fecal samples were vortexed in sterile NaCl and the supernatant was sterile filtered (22µm ...
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bioRxiv - Plant Biology 2020Quote: Tocochromanols in 50.3 ± 0.4 mg DW of ground seed powder were extracted in 750 μL of icecold heptane, using two 3-mm diameter glass beads (Carl Roth GmbH+Co, Karlsruhe, Germany) and a Tissue-Lyser (Qiagen, Hilden, Germany) at 25 Hz for 2 min ...
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bioRxiv - Microbiology 2021Quote: ... 23 Normal colonic mucosal biopsies were placed in lysozyme containing buffer for 30 minutes followed by bead beating and DNA extraction with the Qiagen DNA Blood and Tissue Kit (Qiagen Cat# 69504). The purified DNA samples were stored in aliquots at −20□°C.
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bioRxiv - Microbiology 2021Quote: DNA from plastics was extracted using a modified bead-beating approach combined with the Puregene Tissue DNA extraction kit (Qiagen, Valencia, CA) as previously described 69 ...
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bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Aqueous homogenates of ovarian tissue samples were created by weighing the tissue and adding 4uL of water per mg of tissue and then using a bead beater (TissueLyser II, QIAGEN; Germantown, MD) to homogenize the sample ...
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bioRxiv - Immunology 2022Quote: ... Total DNA was isolated from approximately 200 mg frozen tissue by repeated bead-beating combined with chemical lysis and column-based purification using the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions (Salonen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... tMCAO mice at 24 h and 48 h post reperfusion were used for total mRNA isolation according to the protocols of the Isol-RNA Lysis Reagent (5 PRIME, Gaithersburg, USA) and the bead-milling TissueLyser system (Qiagen, Hilden, Germany). QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 100 μL 0.1mm glass beads were added into the filter casing and put through two 10-minutes cycles of bead beating in a Tissuelyser (Qiagen, Hilden, Germany). The flow-through ...
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bioRxiv - Cell Biology 2022Quote: Snap-frozen iBAT samples were homogenized in deionized water (200 µl per 10 mg tissue) using a TissueLyser bead mill (85210, Qiagen, Hilden, Germany). The glycogen content of the homogenates was measured using a Glycogen Assay Kit II (ab169558 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with sterile glass beads and beaten for 30 s at full speed in a Qiagen Tissue Lyser II (Qiagen, Hilden, Germany®). After cell lysis ...
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bioRxiv - Microbiology 2019Quote: ... The tube was subjected to bead beating for 3 x 45 seconds at 30 m/s in a TissueLyser II (Qiagen, Hilden, Germany) and placed on ice for 2 mins before repeating ...
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bioRxiv - Physiology 2020Quote: Isolated SAN or left atrial tissue was homogenized using a bead homogenizer and total RNA was extracted using QIAzol (Qiagen, Hilden, Germany) and chloroform according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... step for 15 min at 4°C to remove insolubilized material and the supernatant was incubated with Ni-NTA agarose beads (Qiagen, cat: 30230) for 2 hrs ...
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bioRxiv - Microbiology 2020Quote: Ticks were homogenised in a 2 ml reaction tube with two 5 mm steel beads and 500 μl PBS using a Tissue Lyser II (Qiagen, Hilden, Germany) for 3 min at 30 Hz ...
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bioRxiv - Microbiology 2021Quote: ... Another ~0.1 g of tissue was collected from directly below the inoculation site and was ground in bead beater tubes using a PowerLyzer (Qiagen, Hilden, Germany) for two cycles of 2200 rpm for 90 s in each with a 4 min rest between cycles ...
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bioRxiv - Genetics 2020Quote: ... 30 mg of snap frozen tissues were processed adding 1 ml of QIAzol reagent in presence of one 5 mm stainless steel bead (Qiagen, Hilden, Germany). Total RNA isolation from homogenized tissues was performed using Qiagen RNeasy kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.1 g of tissue per 1 mL of PBS was homogenized with stainless steel beads of 5 mm (25–30 Hz for 5 min) by a Tissuelyser (Qiagen, Venlo, Netherlands). The homogenates were centrifuged at 10,000 x g for 10 min and the supernatant was collected and stored at −80 °C until CD quantification ...
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bioRxiv - Genomics 2021Quote: ... then the product was purified with 0.6X SPRIselect beads and washed twice with 80% ethanol and eluted in 30 ul buffer EB (Qiagen, catalog no. 19086). Sequences of primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was subsequently isolated from approximately 30 μg of each tissue using “bead beating” homogenization and an RNAeasy mini-kit (Qiagen, Hilden, Germany) following manufacturer’s directions.
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bioRxiv - Bioengineering 2020Quote: ... The samples were ground by bead beating for 25 sec at 25 Hz using a Tissue Lyser II machine (Qiagen, Hilden, Germany). For CFU-based bacterial enumeration ...
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bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors (PMSF, Aprotinin, Pepstatin, Leupeptin) and homogenised with autoclaved metal beads in a TissueLyser II instrument (Qiagen, Hilden, Germany), followed by ultrasonication (8 sec ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
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bioRxiv - Plant Biology 2022Quote: ... A 2 ml sample of leaf powder was then transferred to an Eppendorf tube and ground to a fine powder using a metal bead in a Tissuelyzer (Qiagen, Germantown, MD). The finely powdered leaf tissue was then sent to Midwest Laboratories (Omaha ...