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Citations for Qiagen :
1901 - 1950 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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bioRxiv - Evolutionary Biology 2021Quote: ... flies were individually crushed in 400 µL of lysis buffer by a sterile 5-mm stainless bead shacked by a TissueLyser® (Qiagen) for 30 s at 25 Hz ...
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bioRxiv - Developmental Biology 2020Quote: ... mUHRF1 C-terminally tagged with GFP- and 6xHis-tag was expressed in HEK 293T cells and then purified using Qiagen Ni-NTA beads (Qiagen #30230). Recombinant mDPPA3 WT and 1-60 were purified as described above ...
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bioRxiv - Immunology 2019Quote: ... approximately 25 mg of tissues were homogenized in 2 mL tubes containing 600 μL of Buffer RLT with 2% β-mercaptoethanol and a stainless steel bead (5 mm, Qiagen) using a TissueLyser II system (Qiagen) ...
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bioRxiv - Immunology 2020Quote: Naso- and oropharyngeal swab samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Immunology 2020Quote: Airway samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from young leaf tissue collected from each F0 parent (flash frozen) and each F2 (desiccated using silica gel) using Qiagen DNEasy reagents and Magattract beads (Qiagen, Inc.). Sequencing libraries for the two F0 parents were prepared using the NEBNext Ultra II kit (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... by shaking with two 3-mm glass beads for 2 min at 30 Hz with a Tissue-Lyser (Qiagen, Hilden, Germany). After 5 min in an ice-cold ultrasonic bath ...
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bioRxiv - Microbiology 2020Quote: ... and the pellet containing virions was resuspended in 200 μl UltraPure water and added to the QIAGEN DNeasy PowerSoil Kit bead tubes (QIAGEN, Germany) for DNA extraction according to the manufacturer’s instructions with one exception ...
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bioRxiv - Microbiology 2021Quote: ... The mycelia were then lyophilized and ground with a tungsten bead in 50 μL lysis buffer (Qiagen DNeasy-Plant AP1 buffer) with a Retsch™-MM400 (Retsch ...
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bioRxiv - Cancer Biology 2020Quote: ... The ligated products were size-selected (AMPure Beads, Agencourt, Beverley, MA) and amplified (GeneRead DNA I Amp Kit, Qiagen, Mississauga, ON); then the regions of interest were captured using biotinylated DNA baits (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... embryo pellets were homogenized by cryo-milling after addition of a 2.5 or 5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 15 seconds 3-6 times ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were powderized by cryomilling after addition of a 2.5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) 3 times at 25 Hz for 30 seconds ...
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RNA-seq sample preparation kits strongly affect transcriptome profiles of a gas-fermenting bacteriumbioRxiv - Systems Biology 2022Quote: ... before disrupting cells with glass beads using the Precellys® 24 instrument and extracting total RNA using the RNeasy Mini Kit (74104; Qiagen). Next ...
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bioRxiv - Genomics 2022Quote: The second lobe of lung or trachea was immersed in 1 mL PBS in a 2 mL Micro Centrifuge Tube (Fisherbrand, 14-666-315) containing one stainless steel bead (5 mm, QIAGEN, 1026563) immediately after dissecting the SARS-CoV-2 infected mouse or hamster ...
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bioRxiv - Genomics 2022Quote: ... Trachea was dissected and immersed in 1 mL PBS in a 2 mL microcentrifuge tube (Fisherbrand, 14-666-315) containing one stainless steel bead (QIAGEN, 69989). After the homogenization ...
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bioRxiv - Genomics 2022Quote: ... The swabs were placed in Red 1.5 mL RINO® screw-cap tubes (NextAdvance) pre-filled with RNase-free zirconium oxide beads and QIAzol Lysis Reagent (Qiagen) was added ...
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bioRxiv - Genetics 2019Quote: ... with 3mm stainless steel beads and RNA was extracted using Qiagen 96 RNeasy Kits according to the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA was eluted in 45 μL of RNase-free water and stored at −70°C ...
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bioRxiv - Genetics 2019Quote: ... The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s ...
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bioRxiv - Cell Biology 2019Quote: ... using 0.6x bead to sample ratio and concentrated by eluting in 60% of the corresponding input sample volume using Elution buffer (Qiagen, 19086). The final sequencing library was quantified using BioAnalyzer High Sensitivity DNA kit (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 8) and the supernatant was incubated with 80 μl pre-washed Ni-NTA coated Sepharose beads (Ni-NTA agarose, (Qiagen, Hilden) for 90 mins at 4°C on a rolling incubator ...
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bioRxiv - Genetics 2020Quote: ... suspended in 1 mL of (v/v) 50% ACN and homogenized in a bead mill (50 Hz, 5 min; TissueLyser LT, Qiagen, Germany) using two 5 mm tungsten balls ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and DNA was extracted using a TissueLyser II bead mill together with the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following supplier recommendations.
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bioRxiv - Microbiology 2019Quote: ... Tissue samples were homogenized with the kit Lysis buffer and 2.8 mm ceramic beads (MO BIO Laboratories Inc., Carlsbad, USA) using TissueLyser II (Qiagen, Hilden, Germany). Subsequently total RNA was extracted as described in the protocol with a DNase I treatment step as per the manufacturer’s instructions ...
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Revisiting a GWAS peak in Arabidopsis thaliana reveals possible confounding by genetic heterogeneitybioRxiv - Genetics 2021Quote: ... Tissue was disrupted by adding two small stainless beads bearings and agitating with a tissue lyser (tissuelyser II, Qiagen, Hilden, Germany) for 10 min at 20 rev/s ...
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bioRxiv - Microbiology 2020Quote: ... the cell supernatant was collected by centrifugation and loaded onto a column packed with Ni-NTA agarose beads (Qiagen, Hilden, Germany). The column was washed with a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Organ samples collected during necropsy were weighed and homogenized in sterile media using beads in a tissue lyser machine (Qiagen, Germany). The lysate was used for RNA extraction using the MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or remaining mosquito bodies were collected separately in a 2ml Eppendorf Safe Lock tube with 250μl DMEM (2% FBS, 1% Sodium Pyruvate) with 25 mg/ml Amphotericin B and a stainless steel bead (Qiagen, Hilden, Germany). Samples were stored at −80°C.
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bioRxiv - Immunology 2021Quote: ... Freshly harvested or in -80°C stored organ in 1 ml complete cell culture medium was homogenized using a metal bead at 25 Hz for 1 - 1.5 min by TissueLyser (Qiagen, Hilden, Germany). After centrifugation at 3500g for 5 min ...
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bioRxiv - Immunology 2022Quote: ... The middle and inferior right lobes were weighted and homogenized with PBS (1:5 w/v) using a 5 mm stainless steel bead (Qiagen, USA) and a TissueLyser LT (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... The frozen plant material was ground to a fine powder using 2 mm Ø glass beads (Huberlab) and a TissueLyser (Qiagen) for 1 min at maximum frequency ...
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bioRxiv - Pathology 2022Quote: ... tissue samples were homogenized in 1 ml of phosphate buffered saline with a metal bead using a TissueLyzer II (Qiagen GmbH). Viral nucleic acids were extracted from blood and homogenized spleen ...
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bioRxiv - Immunology 2022Quote: ... ∼50mg of each thawed sample was transferred to a microcentrifuge tube containing a 5mm stainless steel bead (Qiagen, Part No. 69989) and 1200 µL Buffer RLT Plus ...
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bioRxiv - Neuroscience 2022Quote: ... The beads were rinsed three times with 0.35 M KCl and RNA were eluted in 300 𝜇L RLT buffer (Qiagen, Hilden, Germany) for 5 min at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... and 5mm stainless steel beads (Qiagen, Valencia, CA. RNA was extracted using the RNeasy 96 Universal Tissue Kit (Qiagen, Valencia, CA) and quantified using a NanoDrop 8000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 x 2 minute intervals at 25 Hz with the addition of one 7 mm stainless steel bead (Qiagen #69990). 200 uL of tissue lysate was used for KingFisher Flex (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... buffer containing 100 mM Tris-HCl (pH 8.5), 10 mM TCEP, and 40 mM CAA and crushed using zirconia beads (Tomy, Tokyo, Japan) and TissueLyser (Qiagen, Hilden, German). Proteins were inactivated at 95 °C for 5 min ...
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bioRxiv - Physiology 2023Quote: Whole adipose tissue or isolated primary adipocytes and aorta were mechanically homogenised with 5mm stainless steel beads using a TissueLyser II system (Qiagen, UK) in the presence of 700μL QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... The lysis buffer was then separated from beads and filter material with a syringe and processed using the Qiagen RNeasy extract kit (Qiagen 74104), which included an in-line RNAse-free DNase removal step (Qiagen 79256) ...
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bioRxiv - Microbiology 2023Quote: ... one aliquot was not processed any further and one other aliquot was bead beaten with 100 mg of sterile glass beads (400-600 nm) for 30 s at 30 Hz in a Tissue-lyser II (Qiagen, Germany), in order to open up Symbiodiniaceae cells and release intracellular bacteria to facilitate their growth on agar plates ...
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bioRxiv - Microbiology 2023Quote: ... a small amount of tissue (>25 mg) was added to 1 mL of TRIzol with a single 5mm stainless steel bead (Qiagen, Germany) and homogenised using a TissueLyser II (Qiagen ...
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bioRxiv - Pathology 2023Quote: ... The lyophilized pellets were ground into a fine powder using ∼15 glass beads in a grinding machine (Qiagen MM300 Retsch TissueLyser). A total of 560 DNA samples of isolates coming from separate leaves were extracted using an automated DNA extraction robot ...
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bioRxiv - Microbiology 2023Quote: ... 20-40 mg of tissue was placed in a tube containing 600 μl of OptiMEM and a 5 mm stainless steel bead (Qiagen; #69989) and homogenized in the Qiagen TissueLyser II by two dissociation cycles (1,800 oscillations/minute for 2 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-bead complexes were washed in high and low salt buffer and RNA was eluted from the RNA-bead complexes using the Qiagen RNeasy Plus Micro Kit (Qiagen 74034). Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230) ...
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bioRxiv - Neuroscience 2024Quote: ... Inclusion bodies were solubilized in 8 M guanidine hydrochloride (GdnHCl) and then PrP was captured using Ni-NTA Superflow beads (Qiagen #30410). Bead-bound PrP was refolded on-column using a 4 h gradient from 6 to 0 M GdnHCl and then eluted using a gradient from 0 to 500 mM imidazole ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We placed at least 200mg of feces into individual bead tubes from the kit and then homogenized the sample (Tissue Lyser II, Qiagen, Germany) two times for 20 seconds ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were homogenised in QIAzol reagent (1 mL) in a 2 mL safe lock tube with stainless steel beads using a TissueLyser II homogeniser (Qiagen, UK) at 30 Hz until fully homogenised (1-2 min) ...
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bioRxiv - Evolutionary Biology 2023Quote: Tissue pools were homogenised for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen, Germany). A volume of 0.2× chloroform (Carl Roth ...
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bioRxiv - Molecular Biology 2023Quote: ... One mL TRIzol was added to all samples and homogenized with stainless steel grinding balls for 2 minutes at 30 Hz using a TissueLyser II bead mill (Qiagen, USA). Then ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted after disrupting the frozen leaves with metal beads in RLT and β mercaptoethanol solution using a Qiagen RNeasy plant mini kit (Qiagen) according to manufacturer’s instructions (without the optional DNase step) ...