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Citations for Qiagen :
1751 - 1800 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... peak fractions were supplemented with 10 mM imidazole and 10 µg/mL aprotinin/leupeptin and bound to NiNTA beads (Qiagen) for 2 hours ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease73 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Biochemistry 2022Quote: ... Imidazole pH 8.0 was added to the detergent phase to a final concentration of 15 mM and the mixture was incubated with Ni-NTA agarose beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... mosquitoes were homogenized in 1.7 mL tubes with 50 μL of water and approximately ten 1.0 mm diameter zirconia beads (Thistle Scientific 11079110zx) by shaking at 30 Hz for 2 minutes in a Tissue Lyzer II homogenizer (Qiagen). DNA was extracted using a PureLink Genomic DNA Mini kit (Thermofisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... up to 20mg of tissue immersed in 100µl of Buffer T1 and with the aid of a 5mm steel bead (QIAGEN, #69989) was homogenized in the TissueLyser (QIAGEN ...
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bioRxiv - Plant Biology 2022Quote: ... were added to each sample and samples were homogenized for 10 min in a bead mill (25 Hz, Retsch Qiagen). Samples were centrifuged for 15 min at 14 000 rpm at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... individually crushed and lysed with the help of Mini-Beadbeater-16 from BioSpec using sterile zirconia beads and the DNA was purified using the DNAeasy® blood and Tissue kit (Qiagen, Valencia CA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from LNs by bead homogenization and purified using the Qiagen RNeasy Plus Mini Kit (Qiagen 74104) and cDNA was prepared from 750 ng of RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems 4368814) ...
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bioRxiv - Neuroscience 2022Quote: Total mRNA isolation was performed according to the protocols of the Isol-RNA Lysis Reagent (5PRIME) and the bead-milling TissueLyser system (Qiagen) [40] ...
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bioRxiv - Plant Biology 2023Quote: ... the resulting dried leaves were then ground to powder with a 3-mm glass bead placed in a tube with a Tissuelyser mill (Qiagen). The ground samples were incubated at 4°C in DNA extraction buffer (200 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was homogenized in a bead miller and total RNA was extracted with a Qiagen RNAse microkit (Qiagen, Copenhagen, Denmark). RNA quality was assessed with an Agilent Bioanalyzer and samples with a RIN <8.0 were excluded (n=2).
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 10000g for 10 minutes and the supernatant was allowed to bind with 1 ml of Ni-NTA beads (Qiagen) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... bead beating was performed on 100 mg of cecal contents for 10 min using a tissue lyser (Qiagen, Germantown, MD). Remaining steps for DNA isolation were performed as per manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
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bioRxiv - Physiology 2022Quote: ... They were subsequently organized in metal brackets that were stored at -20°C and homogenized with stainless steel beads at 30 Hz using a TissueLyser II (Qiagen). Samples were lysed twice for one minute ...
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bioRxiv - Immunology 2022Quote: ... For bacterial load determination one lobe of the lung was collected in 1 mL PBS with 1 metal bead and smashed using a tissue lyser (Qiagen). 25 μL of lysed lung were put in duplicates on CYE-plates and grown for 3-4 days at 37°C in a non-CO2 incubator.
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bioRxiv - Biophysics 2022Quote: ... Cell debris was cleared by centrifugation at 40,000 g for 45 min at 4°C and supernatant was applied to His-tag affinity purification using Ni-NTA agarose (beads or 5 ml column, Qiagen) in buffer A (50 mM TRIS ...
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bioRxiv - Microbiology 2023Quote: ... These tissues were then weighed and homogenized by either Omni homogenizer (Omni International, Omni Tissue Homogenizer (TH) 115V) or by 5-mm stainless steel beads with a TissueLyser (Qiagen) and used for onward analysis of infectious virus and vRNA quantification ...
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bioRxiv - Plant Biology 2022Quote: ... frozen in liquid nitrogen, ground to a fine powder (3 mm glass beads added to tissue, ground using tissue lyser (TissueLyser II, QIAGEN), 30/second frequency ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated for 30 min at 4 °C with 5 mL of Ni2+-beads (Qiagen Ni-NTA Superflow) equilibrated in lysis buffer ...
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bioRxiv - Genetics 2023Quote: ... and then the product was purified with 0.6× SPRIselect beads and washed twice with 80% ethanol and eluted in 30 µl of buffer EB (Qiagen, 19086). Sequences of primers ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was eluted by incubation of the beads with 200 μl of RLT buffer from the RNeasy Mini Kit (Qiagen) for 2 min ...
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bioRxiv - Microbiology 2022Quote: ... transferred to 2 ml lysis matrix B tubes (MPBio) and subjected to bead beating for 15 min at 30 Hz (Tissuelyser II, Qiagen) with RNAse A added ...
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bioRxiv - Plant Biology 2023Quote: 8-day-old seedlings growing on agar plates in long-day condition were collected and flash-frozen in liquid nitrogen before powdering with metal beads using a TissueLyser II (Qiagen). Total RNA was isolated using the NucleoSpin® RNA Kit (Thermo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... previously coated with the anti-B55δ antibody (1 hour incubation at 4°C, followed by extensive washes) and Nickel beads (Qiagen) coated with 400 μg of 6XHIS-S67thio-S109A-XeARPP19 ...
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bioRxiv - Genetics 2023Quote: ... were homogenized in G2 solution from NucleoBond buffer kit by a stainless steel bead via the mechanical disruption of TissueLyzer (QIAGEN) for 10min (50 Hz) ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed on the second sample of the beads using Trizol reagent and the miRNeasy mini kit (QIAGEN). An RNase-free DNase (QIAGEN ...
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bioRxiv - Molecular Biology 2023Quote: ... Mosquito bodies and legs were homogenized in phosphate-buffered saline (PBS) supplemented with 20% FBS and 2% penicillin-streptomycin with 5-mm stainless steel beads with a TissueLyser (Qiagen) before RNA isolation ...
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bioRxiv - Plant Biology 2023Quote: ... Arabidopsis thaliana frozen leaf tissues were weighed and ground by using two 3 mm stainless steel beads for 3 minutes at 30 Hz with frozen adapters on a TissueLyser II (Qiagen). The resulting frozen powder was dissolved in 650 µL chloroform-methanol (3:7 ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease40 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Genomics 2023Quote: ... Swabs were incubated at 37 degrees Celsius for 30 minutes then bead beaten for 6 minutes at 30Hz in a TissueLyser II (Qiagen). The rest of the protocol was followed increasing the volumes to account for the additional lysis buffer ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from frozen tissue samples using ceramic beads (Precellys homogenizer, 1× 20 s) in RLT buffer (RNeasy Mini kit, Qiagen) supplemented with 1%ß-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Physiology 2023Quote: ... were immersed in the Transnetyx microbiome sample collection tube (1.5 mL, prefilled with DNA stabilization liquid and glass beads) and sent to Transnetyx (Cordova, TN) for automated DNA extraction (Qiagen DNeasy 96 PowerSoil Pro QIAcube HT extraction kit) ...
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bioRxiv - Microbiology 2023Quote: 25 mg tissue sections from either the left lower lobe of the lung or from the terminal ileum were bead-lysed in RLT buffer (Qiagen) supplemented with 1% (w/w ...
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bioRxiv - Plant Biology 2023Quote: ... 2 h after the light is on) and flash-frozen in liquid nitrogen before powdering with glass beads using a TissueLyser II (Qiagen).
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bioRxiv - Physiology 2023Quote: ... Approximately 25 mg of powdered gastrocnemius were homogenized with pre-chilled metal beads using a TissueLyser II (Qiagen, Hilden, Germany) for 40 seconds at 25 Hz ...
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bioRxiv - Pathology 2023Quote: ... Skeletal muscle (triceps and Tibialis anterior (TA)) and spinal cord tissue samples underwent homogenization with 7 mm stainless steel beads (#69990, Qiagen) in a Tissue Lyser LT (#85600 ...
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bioRxiv - Microbiology 2023Quote: DNA extraction was carried out with 37 mg of freeze-dried mycelium using the Nucleospin Microbial DNA kit in combination with 3 mm tungsten carbide beads (Qiagen) for tissue disruption in a MM 301 vibratory mill ...
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bioRxiv - Cell Biology 2023Quote: ... liver was homogenized in 500uL of 3:1:6 isopropanol:water:ethyl acetate containing internal standard in ceramic bead tubes (Qiagen #13113-50) using the TissueLyzer II (Qiagen #9244420) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and tissuelysed in 100ul of PBS with about ten 1.0mm diameter zirconia/silica beads (Thistle Scientific # 11079110z) using Qiagen TissueLyser II (Qiagen # 85300) for 2 minutes at 30Hz ...
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bioRxiv - Developmental Biology 2023Quote: ... circularized DNA was purified with AMPure XP beads and 4C-libraries were finally generated by two rounds of PCR and purified by QIAGEN column ...
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bioRxiv - Microbiology 2023Quote: ... equal amounts (200 µl) of FruK and Cra cell lysates were mixed with 100 µl of nickel-nitrilotriacetic acid beads (Qiagen). After overnight incubation at 4°C with gentle rotation ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants were then supplemented with 20 mM imidazole and then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) that were pre-washed with 5 column volumes of 1x PBS ...
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bioRxiv - Microbiology 2023Quote: ... RpaB was purified following the same protocol39 using Precellys 24 (Bertin Technologies) to disrupt the cells and 0.5 mL of bedded Ni-NTA agarose beads (Qiagen GmbH) to bind 6×His-tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... The BmAgo3 immunoprecipitated beads were divided into two and incubated in buffer D with/without 200 μg/ml RNase A (Qiagen) at 30°C for 15 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Approximately 50 mg of tissue was homogenised in 500 µl phosphate-buffered saline (PBS) for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen). Total nucleic acids were extracted using the Nucleo Mag Vet Kit (Macherey & Nagel ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were pelleted at 15000 g at 4 °C for 30 minutes and the supernatant was supplemented with 20 mM of imidazole before loading onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Bioengineering 2023Quote: ... Lysate supplemented with 20 mM imidazole was cleared by centrifugation at 15000 g at 4 °C for 30 minutes and the supernatant was then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Microbiology 2023Quote: ... GnTi expressed His-tagged gp140s and gp120s were purified from the harvested and clarified supernatant using Ni-NTA agarose beads (Qiagen) following manufacturer’s instructions.