Labshake search
Citations for Qiagen :
1801 - 1850 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... equal amounts (200 µl) of FruK and Cra cell lysates were mixed with 100 µl of nickel-nitrilotriacetic acid beads (Qiagen). After overnight incubation at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2023Quote: ... tissues were placed in 300μL of sterile PBS containing sterile glass beads and mechanically lysed at a frequency of 20 shakes per seconds for 3 minutes in a TissueLyser II (Qiagen). Negative controls consisted of tubes containing PBS and beads but no sample ...
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bioRxiv - Microbiology 2023Quote: DNA from fecal and cecal samples were isolated using a bead-beating phenol:chloroform extraction method followed by DNeasy Blood & Tissue Kit (QIAGEN, USA). In short ...
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bioRxiv - Microbiology 2023Quote: ... GnTi expressed His-tagged gp140s and gp120s were purified from the harvested and clarified supernatant using Ni-NTA agarose beads (Qiagen) following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: DNA extraction was carried out with 37 mg of freeze-dried mycelium using the Nucleospin Microbial DNA kit in combination with 3 mm tungsten carbide beads (Qiagen) for tissue disruption in a MM 301 vibratory mill ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Approximately 50 mg of tissue was homogenised in 500 µl phosphate-buffered saline (PBS) for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen). Total nucleic acids were extracted using the Nucleo Mag Vet Kit (Macherey & Nagel ...
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Pathology 2024Quote: One frozen liver piece of 25mg per mouse was homogenized in a closed tube with glass beads and 1mL Qiazol by using the Tissue Lyser (Qiagen) for 5min at 50Hz ...
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bioRxiv - Plant Biology 2023Quote: ... samples were flash-frozen in liquid nitrogen immediately prior to grinding and ground using 3 mm glass beads at 30 Hz for 3 min in a TissueLyser II (Qiagen) to a fine powder ...
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bioRxiv - Developmental Biology 2023Quote: ... These were stored at -80°C before RNA extraction using Pre cellys bead homogenisation (Bertin Technologies, France) and RNA easy Kit (Qiagen). Turbo DNA free DNase kit (Ambion ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
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bioRxiv - Microbiology 2024Quote: ... 10 Arabidopsis seedlings from the same MS plate were sampled together in 2 mL microtubes containing two 3 mm-diameter tungsten carbide beads (Qiagen), and flash frozen in liquid nitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... resuspended in 50 ml of distilled water and the protocol was modified to include 0.1 mm glass beads (Qiagen, Germany) to increase DNA extraction by mechanical lysis ...
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bioRxiv - Plant Biology 2024Quote: ... The fresh leaf discs were transferred to a 2 mL tube containing a 5 mm stainless steel bead and 500 µL buffer RLT (QIAGEN). The leaf discs were disrupted using a TissueLyser LT (QIAGEN ...
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bioRxiv - Developmental Biology 2022Quote: ... injected embryos developed to blastocyst stage in culture were collected in 1.5 ul water individually and the whole genome was replicated using a REPLI-g® Mini Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: DNA extractions were performed using ∼10 g of sediment with the PowerMax Soil DNA Isolation Kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). To increase the DNA yield ...
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bioRxiv - Neuroscience 2020Quote: ... 500 GFP-positive and 500 GFP-negative nuclei were sorted directly into 3uL of REPLI-g Advanced Storage buffer (Qiagen Cat#: 150365) in a PCR tube strip (Genessee Cat# ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of strains IMX2300 and IMX2300-1 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Microbiology 2020Quote: ... single Achromatium cells were obtained as previously described (Ionescu et al. 2017) and amplified using the Repli-G kit (Qiagen, Hilden, Germany) following the manufacturer instructions ...
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bioRxiv - Zoology 2021Quote: ... High-molecular-weight genomic DNA was prepared from the kidney of one male shrew using a Genomic-tip 20/G (QIAGEN, Germantown, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... High molecular weight DNA was extracted from 40mg frozen liver from both species using Qiagen Genomic Tip 100/G kit (Qiagen, Hilden, Germany). DNA integrity was assessed visually by separating fragments on a 0.5% TAE agarose gel which revealed a predominant band of high molecular weight DNA >45kb ...
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bioRxiv - Genetics 2022Quote: ... and extracted and amplified from vitrified blastocysts by thawing in phosphate-buffered saline supplemented with 5% bovine serum albumin and processing with REPLI-g whole genome amplification methods (Qiagen, Cat. #150343). Informed consent was obtained from all participants (custodians of the blastocysts ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was extracted from the blood (N. scutatus) or tail clip (P. textilis) using the genomic-tip 100/G kit (Qiagen, Hilden, Germany). This was performed with proteinase K (NEB ...
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bioRxiv - Microbiology 2021Quote: ... total DNAs from these samples (20 g each) were extracted using the Qiagen DNeasy® PowerSoil® Pro Kit (Qiagen, Hilden, Germany) and the integrity of DNA was evaluated by gel electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pelleted at 500 g for 5 min and total RNA was extracted using RNeasy Mini Kit (QIAgen, Cat No. 74104), including DNAse digestion step (QIAgen ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from pelleted nuclei with the Qiagen Genomic tips 20/G and HMW DNA extraction buffer set (Qiagen no. 19060) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Two PowerBead preparations of 0.5 g were used along with the PowerSoil Pro DNA Isolation kit (MoBio Labs/QIAGEN, Carlsbad, CA, USA) according to the instructions ...
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bioRxiv - Genetics 2020Quote: ... DNA binding plate was incubated with 600 μL of liquid for 4 minutes at RT and centrifuged at 1,300 g for 5 minutes sequentially with PB buffer (Qiagen Cat. No. 19066), followed by PE buffer (Qiagen Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... from entire blastocysts (sibling day-8) was whole-genome amplified by multiple displacement amplification (MDA) with a REPLI-g Single Cell Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions with full or half reaction volumes for the fast 3-h protocol ...
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bioRxiv - Microbiology 2021Quote: Soil genomic DNA was extracted from 0.25 g of thawed soil rhizosphere and bulk samples using the DNeasy Power Soil Kit (Qiagen, Germantown, MD, USA). Amount and purity of DNA were assessed using a NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The total genomic DNA was extracted from 0.25 g of soil using the Power Soil DNA Isolation Kit (Qiagen, Carlsbad, CA, USA) in the Microbial Ecology and Biotechnology Laboratory (LEMBiotec ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Genomics 2021Quote: A half-loopful overnight grown bacterial culture was used to extract the genomic DNA using the QIAGEN Genomic-tip 100/G (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... A total of 44 single-sperm samples were selected to undergo whole genome amplification (WGA) with the REPLI-g Single Cell Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... total DNA extraction was performed on this suspension of freshly grown bacterial colonies using the Genomic-tip 500/G kit (Qiagen, Hilden, Germany). Plasmids were electrophoresed in a 0.5% SeaKem Gold agarose with a Tris-acetate buffer ...
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bioRxiv - Microbiology 2022Quote: ... 2 g of soil was immediately transferred into pre-weighed sterile tubes containing 3 ml of LifeGuard soil preservation solution (Qiagen, Toronto, CA) to stabilize the RNA ...
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bioRxiv - Neuroscience 2022Quote: Total forebrain RNA (10 μg) was isolated from three pairs of littermate matched AtrxFoxG1Cre and control P17 mice using the RNeasy Mini kit (Qiagen Cat# 74104). cRNA was generated and hybridized to an Affymetrix Mouse Genome 430 2.0 Array at the London Regional Genomics Center (London ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were thawed and DNA was extracted using the phenol-chloroform protocol detailed above without the liquid nitrogen grinding step (CCS156, CCS165, CCS166 and CCS176) or using the Qiagen Genomic-tip 20/g kit (Qiagen, Venlo, Netherlands) according to manufacturer instruction ...
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bioRxiv - Microbiology 2022Quote: The resulting lysate was then loaded onto a Qiagen Genomic-tip 20/G column and purification was performed following manufacturers recommendations (Qiagen, Hildern, Germany). The Genomic-tip 20/G column was equilibrated with 1 mL of Buffer QBT equilibration buffer (750 mM NaCl ...
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bioRxiv - Genomics 2023Quote: Genomic DNA for the Pacific Bioscience Single Molecule Real-Time (SMRT) sequencing was prepared from 2 mL of fresh blood using the genomic-tip 100/G kit (Qiagen, Hilden, Germany). This was performed with additional RNase (Astral Scientific ...
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bioRxiv - Microbiology 2023Quote: DNA extraction from 0.25 g rhizosphere soil (frozen at −80°C) was performed using the DNeasy PowerSoil Pro kit (QIAGEN GmbH, Hilden, Germany) according to manual ...
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bioRxiv - Neuroscience 2023Quote: Collected microvessels were pelleted at 20,000 g for 10 min and resuspended in 350 μl of RLT Plus Buffer from the RNeasy® Micro Kit (QIAGEN Inc.). Total RNA was isolated using the RNeasy® Micro Kit according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... the samples were centrifuged for 30 min at 24,000 x g and the supernatant was loaded into a Ni-NTA affinity chromatography (Qiagen USA, Germantown, MD). After washing at 20 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 20,000 x g for 45 minutes at 4°C and incubated with Ni-NTA agarose resin (Qiagen, catalogue number: 30230) pre-equilibrated with lysis buffer for 2 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of approximately 0.2 g of biomass pellets were further used for mDNA extraction also with the PowerLyzer® PowerSoil® DNA Isolation kit (QIAGEN).
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bioRxiv - Microbiology 2023Quote: Total soil DNA was extracted from 0.25 g of rhizosphere soil samples using the Power Soil DNA Isolation Kit (Qiagen, Calsbad, CA, USA). The NanoDrop 1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The MDA reaction was performed using the Qiagen REPLI-g Mini kit and purified with the QIAamp DNA Mini kit (Qiagen, Hilden, Germany). Next ...
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bioRxiv - Genomics 2023Quote: ... Total RNA was extracted from approximately 1 g of fresh gametophytic tissue using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA).