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Citations for Qiagen :
2051 - 2100 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Total cellular RNA was extracted using AllPrep DNA/RNA/Protein mini kit (Qiagen, UK). Reverse transcription was carried out using kits from Invitrogen following the manufacturer’s instructions (SuperScript First-Strand Synthesis System for RT-PCR) ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged proteins were purified by affinity chromatography on Ni-NTA Agarose resin (Qiagen) and eluted with buffer containing 0.5 M imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... and day 7,14,21 and 28 of maturation using AllPrep DNA/RNA/Protein (80004, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Total protein was extracted from snap frozen tissues with a Tissue Lyser II (Qiagen) using 5mm Bead Beater balls (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then isolated with Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen # 80004). Breast microstructures from 5 independent donors were exposed to vehicle (PBS and PBS plus DMSO) ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were purified by affinity chromatography with Ni-NTA superflow affinity column (Qiagen) as previously described (33).
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bioRxiv - Cell Biology 2023Quote: ... Proteins harboring the His6 tag were captured by incubation with Ni- NTA resin (Qiagen) (2ml resin per bacterial lysate from 1L culture ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Microbiology 2024Quote: ... Soluble protein fractions were then mixed with pre-equilibrated Ni-NTA agarose resin (Qiagen) and incubated at 4°C for 1 h with rotation ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria isolates then underwent DNA isolation using AllPrep Bacterial DNA/RNA/Protein Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Propidium iodide (10 μg/μl) was added and GFP positive and negative nuclei were sorted directly into 500 μl RLT lysis buffer (Qiagen RNAeasy Plant Mini Kit) containing 1% 2-mercaptoethanol using a BD FACSAria™ III Cell Sorter (BD Biosciences).
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bioRxiv - Microbiology 2019Quote: Community DNA from Lake Matano sediment enrichments was extracted from 2◻g samples and purified using a PowerSoil Isolation Kit and UltraClean® 15 Purification Kit (formerly MO BIO Laboratories, now Qiagen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was cleared by centrifugation (35,000 x g, 1 h) and the supernatant was loaded onto a Ni-affinity chromatography column (Qiagen Ni-NTA Superflow, 20 mL) pre-equilibrated with Ni-NTA buffer ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a 10 mL culture using the QIAGEN® Genomic DNA Buffer Set and Genomic-tips™ 100/G set (midi-prep) (QIAGEN, Hilden, Germany). This procedure adhered to the Sample Preparation and Lysis Protocol for Bacteria as outlined in the QIAGEN® Genomic DNA Handbook ...
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bioRxiv - Microbiology 2020Quote: ... Fresh frozen tissue homogenates were prepared by thawing frozen tissue and placing 200 mg (± 50 mg) of minced tissue in a tube containing 1 ml DMEM culture medium and a steel bead (Qiagen, Germantown, MD, USA). Homogenization was performed with the TissueLyser LT (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... The frozen samples were ground using a bead beater machine (RETSCH, Newtown, PA, USA) followed by total RNA extraction using the RNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: DNA extraction of bacterial DNA from frozen vaginal lavage samples was done using Qiagen spin protocol as per manufacturer’s instructions with an additional bead beating step (Qiagen DNA mini kit, Denmark).
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from all isolates using Qiagen EZ1 Blood extraction kit with a previous step of bead-beating (Qiagen, Crawley, United Kindom). Then total DNA concentration was determined using a Qubit fluorometer (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were homogenised for two cycles of 30 seconds at 25Hz with a 3mm stainless steel bead in a Tissuelyser™ (Qiagen, Manchester, UK). The homogenates were then diluted to a total volume of 1000 µl with RPMI media (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... followed by lysis in a 2 mL sure-lock tube containing 5 mm stainless steel homogenization beads using the TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 seconds at 30 hz followed by 1 min of 30 hz while keeping the sample cold ...
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bioRxiv - Microbiology 2022Quote: ... we added 500 μL of TRIzol and one 3-mm glass bead to the microtubes with mosquitoes and then homogenized the samples in a TissueLyser® (QIAGEN, Hilden, Germany) at 30 Hz for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... Lung homogenates were prepared by homogenizing 200 mg of tissue in a tube containing 1 mL DMEM and a steel bead with a TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 s at 30 hertz ...
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bioRxiv - Microbiology 2019Quote: ... punched papers with faeces or bee samples were homogenized by a stainless-bead (5 mm Ø) in a Tissue Lyser II (Qiagen, Hilden, Germany). Total RNA was eluted in 30 μl RNase-free water and quantified by a Qubit RNA HS kit on the Qubit fluorometer (Thermo fisher scientific ...
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bioRxiv - Biophysics 2022Quote: ... 100 µl of the protein extract were then supplemented with 400 µl of homogenization buffer containing 10 mM imidazole and added to 50 µl Ni2+-NTA agarose beads (Qiagen GmbH, Hilden, Germany) preconditioned with washing buffer (0.1 M sodium phosphate buffer (pH 8.0 ...
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bioRxiv - Genetics 2020Quote: ... approximately 30 seeds per genotype were placed in a 2 mL Eppendorf Safe Lock tube along with one stainless steel bead (Qiagen Cat. No. 69989), frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Plant Biology 2023Quote: ... The frozen tissue samples (∼ 100 mg weighed into a tube with fresh weight recorded) were homogenized in a TissueLyser II bead mill (Qiagen; Cat. No. 85300) for 1 min at 20 Hz without thawing out ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from mock communities cultured in vitro was extracted using a plate-based adaptation of the above protocol including a bead beating process combined with phenol–chloroform isolation with QIAamp 96 DNA QIAcube HT kit (Qiagen Beverly LLC #51331) protocols43.
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from each section individually using the protein precipitation method (Puregene, Qiagen, Netherlands).
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bioRxiv - Molecular Biology 2020Quote: ... Protein was extracted using a modification of the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Uncleaved proteins and TEV were removed by a second pass with Ni-NTA resin (Qiagen). Proteins were concentrated and further purified using gel filtration (S75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... CRY2 protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen, Hilden, Germany) following elution with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The His-tag protein was purified by using a column of Ni-Sepharose 4B (Qiagen), followed by gel filtration using a Superdex 75 column (G.E ...
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bioRxiv - Systems Biology 2020Quote: ... Upstream regulation and protein networks were evaluated via Ingenuity Pathway Analysis (IPA, QIAGEN, Hilden, Germany) based on the DEPs in the plasma experiment ...
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bioRxiv - Microbiology 2021Quote: ... The OrhR-His6 fusion protein was purified to homogeneity using Ni-NTA Spin Columns (Qiagen) and dialyzed against binding buffer ...
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bioRxiv - Microbiology 2019Quote: ... The extraction was carried out using the AllPrep Bacterial DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were purified under native conditions by affinity chromatography using Ni–NTA agarose (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... and the recombinant protein was purified on Ni-NTA agarose following the manfacturer’s instructions (Qiagen). The eluate (3x 500 μl ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from clarified lysate by affinity chromatography on Ni2+-nitrilotriaceta-agarose (Qiagen) in 20 mM Tris-HCl 500 mM NaCl pH 8.0 buffer and by size exclusion chromatography on Superdex 200 HL 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Protein purification was carried out using QIAExpressionist NiNTA purification kit according manufacturer’s instructions (Qiagen, Germany) and following the HIS purification under native conditions protocol ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant Lsr2 proteins were purified using immobilized-metal affinity chromatography (Ni-NTA agarose, Qiagen) and size exclusion chromatography ...
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bioRxiv - Biophysics 2019Quote: ... All the proteins were obtained in cell lysates and subsequently purified using Ni-NTA (Qiagen) affinity-columns and superdex 200 increase 10/300 GL (GE Lifesciences ...
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bioRxiv - Molecular Biology 2019Quote: ... His6- and GST-tagged proteins were affinity-purified in batch on Ni-NTA agarose (Qiagen) and glutathione Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from fetal tissues using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) and bisulfite conversion was carried out using EZ DNA Methylation Kits (Zymo Research) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was subsequently applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) which was washed with dialysis buffer supplemented with increasing concentrations of imidazole (10-250 mM) ...
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bioRxiv - Microbiology 2020Quote: ... 6x Histagged proteins were purified from the crude extract using Ni-NTA agarose columns (Qiagen) according manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... NspS-6xHis protein was purified from the clarified supernatant using Ni-NTA Superflow resin (Qiagen) equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: Breast tumor tissues (PTs and IBTRs) were processed using the AllPrep DNA/RNA/Protein (Qiagen) protocol ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...