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Citations for Qiagen :
1951 - 2000 of 3015 citations for 1 2 2 morpholin 4 ium 4 ylethoxy phenyl butan 1 one chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The SCGs cultured in 6-well dishes and one entire 6-well was harvested and pooled together for RNA isolation (RNeasy, Qiagen) per sample ...
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bioRxiv - Neuroscience 2022Quote: Vector genomes present in each treated animal was quantified after preparation of genomic DNA from one brain hemisphere with the AllPrep96 DNA/RNA Kit (Qiagen). qPCR with primers and a probe directed against the hSyn promoter sequence (forward 5’-ATGAGTGCAAGTGGGTTTTAGGA-3’ ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen). Purified mRNA was converted to cDNA with the High Capacity cDNA Reverse Transcription kit (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell free DNA (cfDNA) extraction and library preparation was performed on plasma samples (QIAseqTM cfDNA All-in-One Kit (QIAGEN)) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by three rounds of washes with sterile PBS + PI for 45 mins, then subjected to one round of incubation with DNase I (4716728001, MilliporeSigma) + RNase A (19101, Qiagen) + PI for 72 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Viral RNA was extracted one from (Used in detection limit evaluation) using viral RNeasy® Mini-kit (Qiagen, Hildan, Germany). Extracted RNA was stored at −70°C until further use.
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bioRxiv - Physiology 2023Quote: One thyroid lobe was used for total RNA extraction according to the manufacturer’s protocol (RNeasy Micro Kit, Qiagen, Hilden, Germany). RNA concentration and purity were determined by spectrophotometry (ratio 260/280 nm) ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Transcript-specific primers were designed to span introns and the spcar3 mutation site and used in one-step RT-PCR reactions (210210, OneStep RT-PCR kit, Qiagen). Primer sequences for qRT-PCR are listed in Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... and Chl523R (5’ CCY YMC GTA TTA CCG CAG CT 3’) targeting the 16S rRNA gene on a QIAcuity One digital PCR device (Qiagen) as recommended by the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... total RNA of each developmental stage was isolated from one to three biological replicates using the RNeasy Micro Kit (Qiagen). Library preparation for stranded polyA-based mRNA was then performed using the TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... One hundred microliters (uL) PBS was added to NW pellets followed by 280uL AVL lysis buffer (Qiagen, Germantown, MD, USA). Two to three mL blood was collected from the cranial vena cava in an SST tube for serum separation ...
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bioRxiv - Molecular Biology 2020Quote: ... and clarified lysate was incubated with 1 ml packed Ni-NTA resin (QIAGEN) per 750 ml culture for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... The embryos were treated with protease (1 µL of 25 µg/µL Qiagen Protease ...
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bioRxiv - Cell Biology 2019Quote: Decellularized scaffolds were homogenized-lysed for 1 hr in Tissue homozilyser II (Qiagen). The ECM proteins were analyzed on SDS PAGE ...
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bioRxiv - Microbiology 2019Quote: ... perfringens cultures (maximum of 1 x 107cells) lysed with lysis buffer (200μl Qiagen Buffer P1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of genomic DNA extracted using the DNeasy Blood & Tissue kit(Qiagen) was bisulfite-converted by using the EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from 1 × 107 PBMC using RNeasy mini kit (Qiagen, Hilden ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the plugs were treated with RNase (Qiagen, 20µl in 1 ml TE buffer) for 1h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... weighed and homogenized in 1 ml of DMEM with stainless steel beads (QIAGEN) for 30s at 30 Hz using TissueLyserII (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was mixed with 1 mL of Ni-NTA agarose beads (Qiagen), and the mixture was rotated at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of total RNA was retrotranscribed using QuantiTect Reverse Transcription kit (Qiagen). Primers designed to amplify cpxP ...
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bioRxiv - Neuroscience 2020Quote: Antennae from twenty moths were homogenized in 1 mL QIAzol (Qiagen, Hilden, Germany). RNA was extracted using Qiagen RNeasy Mini kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of total RNA extracted with the RNeasy Plant Mini Kit (Qiagen) was treated with DNaseI (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were then incubated with 1 mL prewashed Ni-NTA resin (Qiagen) at 4 °C for 2 h on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellets were resuspended in 1 ml Qiagen RNA protect reagent (Qiagen) and incubated for 24 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: RPE-1 p53KD cells (parental and clones) were harvested in buffer RLT (Qiagen). Strand-specific libraries were generated using the TruSeq PolyA Stranded mRNA sample preparation kit (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 µg total RNA was reverse transcribed using the Quantitect kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Two independent siRNAs targeting YBX1 (Hs_YBX1-1 and Hs_YBX1-6 FlexiTube siRNA, Qiagen) and a non-targeting negative control siRNA (AllStars ...
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bioRxiv - Microbiology 2022Quote: ... and tissues were homogenized in 600 μl RLT buffer and 1% betamercaptoethanol (Qiagen) in a 2 mL cryovial using a tissue lyser (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel (Qiagen, USA). The bands corresponding to full-length gene segments were excised and the DNA was recovered using QIAquick gel extraction kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... a penta-His HRP conjugate was used (1:4000, QIAGEN GmbH, Hilden, Germany). The detection of GFP was performed with the primary antibody anti-GFP (1:10000 ...
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bioRxiv - Biochemistry 2021Quote: ... peritoneal membrane sections (80 mg) were dissociated in 1 ml buffer RLT (QIAGEN) supplemented with β-mercaptoethanol (1:100 v:v ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μL.mL−1 NP-40) and immediately placed in “RTL plus” buffer (Qiagen). mRNA was purified using RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (1 μg) was reverse-transcribed by QuantiTect Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Tissue was further homogenized at 28 Hz for 1 min using TissueLyser (Qiagen) followed by 45 min incubation at 65°C ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was then immediately added to 1 mL RNAprotect Bacteria Reagent (Qiagen), vortexed ...
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bioRxiv - Molecular Biology 2020Quote: ... 200ul and 600ul Buffer RLT (containing 1% β-mercaptoethanol, QIAGEN RNeasy Mini Kit) was added to nuclear and cytosolic fractions respectively and vortexed vigorously ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from 1×106 cells with the RNeasy Mini Kit (Qiagen). For qPCR ...
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bioRxiv - Cell Biology 2022Quote: Aliquots (1-1.2 ml) of culture were added to RNAProtect Bacteria Reagent (Qiagen) (2-2.4 ml) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Figure 1) stored in RNAlater using the AllPrep DNA/RNA mini kit (Qiagen). Purity and quality of the extracted DNA was estimated using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in 1 mL of RNAprotect solution (Qiagen, Hilden, Germany), pelleted and stored at −80°C until gDNA extraction ...
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bioRxiv - Immunology 2019Quote: Total RNA from 1×106 cells was isolated using RNeasy Mini kit (Qiagen). 50 b.p ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer then eluted in buffer containing 250 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was treated on-column with 1 µL RNase-Free DNase (Qiagen #79254). First strand cDNA was synthesized from purified total RNA using the SuperScript VILO cDNA Synthesis Kit (Invitrogen #11755050 ...
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bioRxiv - Microbiology 2019Quote: ... and the bacterial pellets resuspended in 1 ml RNA Protect Bacterial Reagent (Qiagen). Following another centrifugation cycle ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to a 1 mL of Ni-NTA Agarose (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to a 1 mL of Ni-NTA Agarose (Qiagen) equilibrated with lysis buffer ...