Labshake search
Citations for Qiagen :
1851 - 1900 of 3015 citations for 1 2 2 morpholin 4 ium 4 ylethoxy phenyl butan 1 one chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... One microgram of RNA from each sample was used for cDNA synthesis by using QuantiTect Reverse Transcription Kit (QIAGEN). Quantitative PCR was performed using SYBR Green PCR Master Mix in QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Microbiology 2021Quote: ... After another one-hour incubation bacteria were pelleted and total RNA was extracted using the miRNeasy mini kit (QIAGEN). RNA samples were treated with RNase-Free DNase (QIAGEN ...
-
bioRxiv - Microbiology 2021Quote: One-tenth of a TG single cell suspension was used for DNA extraction using the QIAamp DNA Kit (Qiagen). TaqMan qPCR was performed in duplicate on the 7500 Real-Time PCR system using 2X PCR Universal Master Mix (Applied Biosystems ...
-
bioRxiv - Immunology 2020Quote: ... which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen, Hilden, Germany), 0.1 mM dithiothreitol (Invitrogen ...
-
bioRxiv - Genomics 2021Quote: Total DNA was extracted from muscle tissue of one male fish using a QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV2 subgenomic viral RNA was quantified using primer probe sets as previously described (Wölfel et al., 2020) and Quantifast One-Step RT-PCR master mix (Qiagen) on a QuantStudio 3 or 5 instrument (ThermoFisher) ...
-
bioRxiv - Systems Biology 2022Quote: ... genomic DNA was extracted for pelleted cell fractions with one of the following: DNeasy Blood & Tissue Kit (Qiagen #69504) for fractions with fewer than 5 x 106 cells ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was isolated one week after the initial passage using Trizol/Chloroform extraction and RNeasy Mini kit (Qiagen) purification ...
-
bioRxiv - Biophysics 2022Quote: ... HEK-293 cells were seeded in Labtek chambers (#1.5 glass-bottom) one day in advance and then transfected with Effectene Transfection Reagent (QIAGEN) according to the manufacturer’s protocol.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Allele-specific expression was measured using RT-ddPCR with the One-Step RT-ddPCR Advanced Kit for Probes (Qiagen) on a QX200 ddPCR Droplet Reader (BioRad ...
-
bioRxiv - Microbiology 2024Quote: ... used for DNA extraction of minipig blood and (NM-3) one negative control for the kit DNeasy PowerWater (Qiagen) used for the DNA extraction of water samples ...
-
bioRxiv - Cell Biology 2020Quote: RNA was extracted from 1 million cells with RNeasy mini Kit (Qiagen) following the manufacturer’s instructions and with DNase treatment on column ...
-
bioRxiv - Developmental Biology 2021Quote: RNA was isolated from 1×103-2.5×106 cells using QIAzol (Qiagen). Total RNA was extracted using miRNeasy Mini kit (Qiagen) ...
-
bioRxiv - Microbiology 2020Quote: ... and the pellet was immediately resuspended in 1 mL RNA-Protect (Qiagen). RNA-stabilized cell pellets were stored at −80 °C.
-
bioRxiv - Microbiology 2020Quote: ... 1 mL was centrifuged and washed twice with nuclease-free water (Qiagen). The cell pellet was resuspended in 500 µL of nuclease-free water ...
-
bioRxiv - Biochemistry 2020Quote: ... PARP-1 was purified by sequential application to Ni-NTA agarose (Qiagen), HiTrap Heparin HP column (GE Healthcare) ...
-
bioRxiv - Cell Biology 2020Quote: ... was carried out after treatment with 50 μg ml−1 RNase (Qiagen) for 20 min at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... with 1 µL cDNA using the following oligonucleotides (QuantiTect Primer Assays, Qiagen): Gli1 (Gli1 ...
-
bioRxiv - Neuroscience 2020Quote: ... and 1% Triton X-100 and homogenized in a TissueLyser II (Qiagen), centrifuged at 20,000 rcf at 4°C for 10 minutes after which the supernatant was collected for multiplexed immunoassay analyses.
-
bioRxiv - Plant Biology 2021Quote: ... Genomic DNA (1 μg) isolated using a DNeasy plant mini kit (Qiagen) was used for library construction ...
-
bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... The followed antibodies and dilutions were used: Strep 1:1000 (Qiagen, 34850), PAF1 1:1000 (Bethyl Labs #A300-173A) ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA (1 μg) was was extracted using miRNeasy Mini Kit (Qiagen) and applied to either miR-based or mRNA based reverse transcription ...
-
bioRxiv - Biochemistry 2020Quote: ... we took 1 µg of plasmid (typically from a midi-prep (Qiagen)) and digested in a 20 µL reaction with 20 units of BamHI-HF (NEB ...
-
bioRxiv - Biophysics 2020Quote: ... the surface was incubated with 1 nM biotinylated penta-His antibody (Qiagen) in buffer A for 10 min ...
-
bioRxiv - Genomics 2022Quote: ... total RNA was extracted from 1 million cells using RNeasy columns (QIAGEN). Ribosomal RNA (rRNA ...
-
bioRxiv - Microbiology 2020Quote: ... and IFN-stimulated DF-1 and CEF using the RNeasy kit (Qiagen) according to the manufacturer’s instructions as previously described48 ...
-
bioRxiv - Immunology 2021Quote: ... the supernatant was loaded onto a 1 ml Strep-Tactin column (Qiagen) at a flow rate of 0.7 ml/min in the ÄKTA prime-plus liquid chromatography system (GE Healthcare) ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 µg RNA was reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... The clarified lysate was incubated with 1 ml Ni-NTA resin (Qiagen) for 1 h ...
-
bioRxiv - Biochemistry 2022Quote: Aliquots (1 ml) of culture were added to RNAProtect Bacteria Reagent (Qiagen) (2 ml) ...
-
bioRxiv - Genomics 2020Quote: ... and 1 μl of diluted (0.08x) QIAseq FastSelect 5S/16S/23S (Qiagen) were added into 6 μl COVID-19 specimen RNA along with 3 μl NA denaturation buffer ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using miScript II RT kit (Qiagen) as per protocol before qPCR using miScript SYBR Green kit (Qiagen ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 1 μM Locked Nucleic Acid (LNA) oligo-d(T)30 (Qiagen) in primary-probe hybridization buffer composed of 40% formamide (Sigma ...
-
bioRxiv - Molecular Biology 2019Quote: ... A 1:3 mixture of a QuantiFast SYBR Green PCR Kit (Qiagen) and a FastStart SYBR Green Master (Roche ...
-
bioRxiv - Cell Biology 2019Quote: ... Two hundred microliters of 1-mg/ml RNase A (Qiagen, Germantown, MD) was added ...
-
bioRxiv - Genetics 2021Quote: ... for less than 1×104 cells or the RNeasy kit (Qiagen #74004) otherwise ...
-
bioRxiv - Microbiology 2020Quote: ... harvested pellets were resuspended in 1 ml RNA Protect Bacteria Reagent (Qiagen), incubated for 5 min at ambient temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Each 1-kb fragment was purified using a gel extraction kit (Qiagen) and were ligated by PCR using primers Lpg499-DR and Lpg499-UF to generate a 3-kb fragment that was purified by a gel extraction kit (Qiagen) ...
-
bioRxiv - Neuroscience 2021Quote: ... 10 mM imidazole (final concentration) and 1 ml Ni-NTA resin (Qiagen) pre-washed in 25mM Hepes ...
-
bioRxiv - Microbiology 2021Quote: ... multivorans 1 genomic DNA (gDNA, extracted with DNeasy Blood & Tissue Kit, Qiagen), cleaned up using the Monarch PCR Purification kit (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2021Quote: ... amplicons were subjected to the QIAseq 1-Step Amplicon Library Kit (Qiagen) for Illumina compatible next generation sequencing (NGS ...
-
Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... containing 10μl of 1% v/v β-mercaptoethanol TCL buffer (Qiagen # 1031576) using a BD AriaII cell sorter ...
-
bioRxiv - Immunology 2020Quote: ... Tissue was homogenized at 30hz for 1 minute in a TissueLyser (Qiagen) then briefly spun at maximum RPM in a benchtop centrifuge to pellet large debris ...
-
bioRxiv - Genomics 2020Quote: ... and purified from a 1% agarose gel (QIAquick Gel Extraction Kit, Qiagen).
-
bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from 1 million cells by RNeasy columns (QIAGEN). We used 2 µg of RNA to perform reverse transcription with random primers using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech ...
-
bioRxiv - Microbiology 2022Quote: ... The lysate was incubated with 1 mL of Ni- NTA agarose (Qiagen) for 1 h at 25 °C with rotating ...
-
bioRxiv - Plant Biology 2022Quote: ... The supernatant was mixed with 1 ml Ni-NTA-Agarose slurry (Qiagen) in an overhead rotator (10 rpm ...
-
bioRxiv - Microbiology 2023Quote: ... Dilutions of 104-101 plasmid copies contained 1 ng carrier RNA (QIAGEN). QPCR was performed in a LightCycler 480 Instrument II (Roche ...
-
bioRxiv - Immunology 2022Quote: ... RNA was extracted from 1 × 106 cells using the RNeasy kit (Qiagen), and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831 ...