Labshake search
Citations for Qiagen :
2001 - 2050 of 3015 citations for 1 2 2 morpholin 4 ium 4 ylethoxy phenyl butan 1 one chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was incubated for 1 hour with Ni-NTA agarose beads (Qiagen) at 4°C ...
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bioRxiv - Genetics 2019Quote: ... 20 pmol of each primer and 1 U of Taq polymerase (Qiagen, USA) and 1 µl of the DNA template ...
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bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Molecular Biology 2023Quote: The eluted protein was mixed with 1 ml Strep-Tactin Superflow agarose (Qiagen) and incubated at 4 °C for 30 min ...
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bioRxiv - Immunology 2022Quote: Total RNA (from 1 × 106 cells) was isolated with the RNeasy kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % (w/v) sodium deoxycholate) and once with 500 µl EB buffer (Qiagen). Beads were treated with 300 µg/ml RNase A ...
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bioRxiv - Neuroscience 2022Quote: ... 1% of input was saved in RLTplus buffer (Qiagen RNeasy Micro Kit #74034) supplemented with 2-Mercaptoethanol before 20µl or 15µl of HA-magnetic beads (Pierce ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 1 mL of Ni-NTA beads (Qiagen, USA) while rotating for 2 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The worms were then suspended in 1 ml RLT buffer (Qiagen RNeasy kit) containing β-mercaptoethanol and flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was added to a 1-mL Ni-NTA agarose column (QIAGEN). The column-bound HigB2–HigA2 complex was disrupted by washing the column with buffer containing 5 M guanidine-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... + 1% β-mercapto-ethanol and RNA was purified using the RNeasy kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... This was then added to 1 ml of Ni-NTA Agarose (Qiagen, 30310) and left on a rolling platform at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was incubated for 1 hr with Ni-NTA agarose beads (Qiagen) equilibrated in lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... using specific primers (Supplemental Table 1) in a Rotor-Gene Q machine (Qiagen). Standard curves were achieved by dilutions of one cDNA sample ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 1 ml of Ni-NTA agarose beads (Qiagen), equilibrated in the lysis buffer for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a 1 ml Ni-NTA Superflow cartridge (QIAGEN). The column was washed with 10 column volumes (CVs ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA from 1×106 cell was first extracted with RNeasy Plus kit (Qiagen), per manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... RNA (1 μg) was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... with zirconia/silica beads in 1 mL RLT buffer (Qiagen, Germantown, MD, USA). RNA was extracted using the Zymo Direct-zol RNA Miniprep Plus kit as recommended by the manufacturer ...
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bioRxiv - Genetics 2024Quote: ... the cells were lysed in 1 mL of RLT buffer obtained from Qiagen, and for Mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells at a concentration of 1 μg using Effectene transfection reagent (QIAGEN). Cells were infected with Mimivirus (MOI-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μg plasmid DNA (1 μg/μL; prepared by QIAGEN Plasmid Midi Kit) in 30 μL MMG solution was used to transform 200 μL protoplasts by gently mixing with 230μL 40% PEG transformation buffer ...
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bioRxiv - Cell Biology 2024Quote: ... containing 1% β-mercaptoethanol and homogenized using a bead mill (Qiagen TissueLyser LT). RNA was purified using the Qiagen RNeasy Mini kit with DNase (Macherey-Nagel ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: RPE-1 p53KD cells (parental and clones) were harvested in buffer RLT (Qiagen). Strand-specific libraries were generated using the TruSeq PolyA Stranded mRNA sample preparation kit (Illumina) ...
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bioRxiv - Genomics 2024Quote: ... 1 h 45 min] and resuspended in 360 µL of buffer ATL (Qiagen). DNA was extracted in duplicate with the QIAamp DNA Mini Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: Total RNA (from 1 × 106 cells) was isolated with the RNeasy kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Immunology 2021Quote: ... BALF and serum was performed by quantitative reverse-transcriptase PCR (qRT-PCR) using the one-step Quantinova™ Probe RT-PCR kit (QIAGEN). Primers and a TaqMan probe were designed to hybridize to a sequence in ORF7 (encoding N) ...
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bioRxiv - Bioengineering 2019Quote: Genes were quantified by one-step quantitative reverse transcription-polymerase chain reaction (q-PCR) using the QuantiFast SYBR Green RT-PCR kit (Qiagen, UK). A master mix was prepared by mixing 6.25 μl SYBR green ...
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bioRxiv - Genomics 2020Quote: ... and from two “normal” males (50289,70203) and one “normal” female (20025) were collected and stored in RNAlater™ (QIAGEN, Hilden, Germany). The normal individuals may be carriers of the genetic variant(s ...
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bioRxiv - Genetics 2020Quote: ... 1μl of bisulfite converted DNA was used as template to amplify target genomic regions using specific primers (one biotinylated) with the PyroMark PCR kit (Qiagen, #978703), following the recommended conditions (annealing temperature 56°C) ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA was extracted from overnight cultures from one isolated colony of each stock following standard protocols (QIAGEN DNAeasy, Hilden, Germany). Extracted DNA was treated with RNase A and purified on Axygen AxyPrep Mag PCR Clean-up SPRI beads ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: ... All RNA extracts were subjected to reverse transcription polymerase chain reaction (RT-PCR) using the one-step RT-PCR kit (Qiagen, USA) and PanCoV F2 (5’-AAR TTY TAY GGH GGY TGG-3’ ...
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bioRxiv - Microbiology 2020Quote: ... These primers were used to amplify the mRNA from the sample using Quantifast SyBR Green one –step RT-PCR kit (Qiagen, Netherlands) and the reactions were run using ABI 7500 real-time PCR instrument (Thermo Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification of a single product of the expected size by a primer pair was first confirmed by RT-PCR using Qiagen® One-Step RT-PCR kit (Qiagen) followed by agarose gel analysis of the amplified products ...
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bioRxiv - Microbiology 2019Quote: ... 1 µM of forward and reverse oligos were heated for one minute at 100°C with 5 mM MgCl2 and 7 mM Tris-Cl (i.e. Qiagen Elution Buffer) and annealed by slowly cooling to room temperature.
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bioRxiv - Cell Biology 2020Quote: Cells were seeded in 6-well plates at a density of 80,000 cells/well one day prior to transfection and transfected with DR5-AS LNA GapmeR (Qiagen, United States) at a concentration of 40 nM or with 1500 ng of pcDNA3.1-DR5-AS with the Fugene HD transfection reagent (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sample was divided into 5 parts and each part was purified using one PCR purification columns (PCR purification kit, Qiagen 28106). 50ul elution buffer was used the elute each column ...
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bioRxiv - Cell Biology 2022Quote: ... and organoids at the end of passage one subjected to two weeks of differentiation (n=3) using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: The frozen filters were retrieved and cut into two halves using sterile surgical blades and DNA was extracted from one-half using DNeasy PowerSoil Kit (Qiagen, Germany) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was quantified and qualitatively assessed using a nanodrop instrument following which one step qRT-PCR was performed using QuantiFast SYBR Green PCR kit (204054; Qiagen, Germany). Table 1 lists the primers used for mRNA quantification and cellular glyceraldehyde-3-phosphate dehydrogenase expression was used as a normalizing control ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was divided into two parts: one part was directly combined with nickel-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen, China); the other part was heated in a 65 °C water bath for 60 minutes and then centrifuged at 14000 rpm for 60 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... while URA3 and ACT1 levels were measured by one-step RT-PCR with specific primers (Supplementary Table S3) following the standard protocol of the RNeasy® Mini kit (Qiagen). All strains were propagated for at least 100 generations before analysis.
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bioRxiv - Physiology 2020Quote: ... One μg of total RNA was reverse transcribed into cDNA using the Qiagen QuantiTect Reverse Transcription Kit (Qiagen, Valencia, California, USA) following manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... 5 ng of each PCR product was pooled into one tube for purification using the QIAquick PCR purification kit (Qiagen #28106) and eluted into 30 µL Nuclease Free Water ...
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bioRxiv - Genomics 2020Quote: ... high-molecular weight DNA was extracted from the entire body of one F1 pupa using a QIAGEN Blood & Culture DNA Midi Kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was recovered from one-month-old of the DL1YTT001 culture by using a RNeasy® PowerSoil® kit (Qiagen). The kits mentioned above were used according to manufacturer protocols.
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bioRxiv - Physiology 2023Quote: ... A one-step real-time quantitative polymerase chain reaction (RT-qPCR) was performed using a QuantiFast SYBR Green RT-PCR one-step kit on a Rotorgene 3000Q thermocycler (Qiagen, UK). Each reaction was setup as follows ...
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bioRxiv - Developmental Biology 2023Quote: DNA from two separate stage 42 embryos of F0 Cab and F0 Kaga and one stage 42 embryo of F1 hybrid Kaga/Cab was extracted using DNeasy Blood and Tissue Kit (Qiagen #69504) following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 3 × 105 cells.well−1 in 6-well plates and transfected 24 h later with one pmiRGLO derivative construct per well (0.9 µg plasmid) using Effectene (Qiagen cat. #301425). After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat ...
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bioRxiv - Microbiology 2023Quote: Total DNA was isolated from the whale blow and technical control samples in one batch using a QIAamp DNA Mini Kit (QIAGEN, Germany). The primers used for sequencing the 16SrRNA V3 and V4 regions were 341F (5’-CCTACGGGNGGCWGCAG ...