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Citations for Qiagen :
1601 - 1650 of 5919 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was isolated using Genomic DNA Buffer Set with Genomic-tip 20/G (Qiagen, Hilden, Germany). To construct the complementation vector ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real time PCR (qRT-PCR) was performed in duplicate using a SYBR® Green PCR kit (Qiagen) on a Mastercycler® ep realplex (Eppendorf ...
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bioRxiv - Immunology 2020Quote: ... The number of PCR amplification cycles was determined by qRT-PCR using Quanitfast SYBR Green PCR mastermix (Qiagen) and Nextera Primer I5 and I7 Indexes for 5 cycles ...
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bioRxiv - Genomics 2023Quote: ... The number of PCR cycles in the second PCR was determined with QuantiTect SYBR Green PCR Kit (Qiagen) using 1 μL of the first PCR product after 5-folds of dilution or H2O as the template with 0.3 μM concentration of each primer in 10 μL reaction volume in a duplicate measurement setting ...
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bioRxiv - Neuroscience 2020Quote: ... Complementary DNA (cDNA) was prepared using Qiagen Omniscript RT Kit (205111, Qiagen). Mouse and rat gene expression was normalized to the internal control Hsp90 and β-actin ...
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bioRxiv - Immunology 2021Quote: ... RNA was reverse transcribed with the QuantiTect reverse transcription (RT) kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Generation of cDNA was performed by GoTaq 2-step RT system (Qiagen). Real-time PCR reactions were measured by ABI StepOnePlus system using SYBR green qPCR master mix (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription of miRNAs was performed with the miScript RT Kit (Qiagen) and qRT-PCR was performed with the Power SYBR Green-PCR Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesis was performed with an miScript II RT Kit (218161, Qiagen) using HiFlex buffer according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and RT-qPCR was performed using Quantitect SYBRgreen qPCR master mix (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: cDNA was synthesized using miScript II RT Kit (Qiagen, Cat No. 218161), according to manufacturer specifications ...
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bioRxiv - Microbiology 2019Quote: ... We eliminated DNA from RNA samples using the RTS DNase kit (QIAGEN) and synthesized first strand cDNA using the SuperScript III First Strand Synthesis Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed into cDNA using miScript II RT kit (Qiagen). cDNA was then processed using miScript SYBR Green PCR kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using miScript II RT kit (Qiagen) as per protocol before qPCR using miScript SYBR Green kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with random hexamers using an Omniscript RT Kit (Qiagen). cDNA was quantified using TaqMan gene expression probes (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Synthesis of cDNA was carried out using an Omniscript RT kit (Qiagen) with 100 ng total RNA per reaction ...
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer’s protocol (Qiagen Omniscript RT, Cat No./ID: 205111). Afterwards we performed qPCR to assess the mRNA expression of atossa and porthos ...
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bioRxiv - Immunology 2020Quote: ... 2μg was utilised for cDNA synthesis using the Omniscript RT kit (Qiagen). Quantitative PCR was carried out using previously described specific primers and probes and normalized to 18S rRNA housekeeping gene ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were run on a Rotor-Gene Q cycler (Qiagen) in a three-step protocol ...
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bioRxiv - Genomics 2019Quote: ... and RT-qPCR: RNA extraction was performed using an RNAeasy kit (Qiagen). Reverse transcription was performed using Superscript III (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... and then converted to cDNA using the miRCURY LNA RT Kit (Qiagen). The samples with total exosomal RNA and cDNA were stored in a freezer at −80℃ ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using QuantiNova SYBR Green 1 Step kit (Qiagen) with 40 cycles of amplification at 57ᵒC performed on an ABI 7500 (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: cDNA synthesis was performed using the miScript II RT Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA was reverse transcribed using the miScript-II RT kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The complementary DNA was synthesized by a miScript II RT kit (Qiagen) using the hiSpec buffer method ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently reverse transcribed using the miRCURY LNA RT kit (Qiagen, 339340) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently reverse transcribed using the mScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... was used to synthesise cDNA and miRCURY LNA RT Kit (Qiagen, UK) was used to perform qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... The NS segment was amplified using the Omniscript RT Kit (Qiagen, Germany) and Phusion® High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: Reverse transcription was performed using the Omniscript RT Kit (Qiagen Inc., USA). RNA samples were thawed on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Mice received either miRCURY LNA power inhibitor for mmu-miR-335-5p (Ant-335; sequence: 5’-CATTTTTCGTTATTGCTCTTG-3’; Qiagen, Cat No.: 339132; 0.1 nmol in 2 μL PBS), or a non-targeting scrambled control (Scr ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted in 150 μl phenol-chloroform and purified on a QIAquick column (Qiagen) and eluted in 50 μl EB buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Immunology 2022Quote: ... then 150 µL of homogenate was added to an equal volume of buffer RLT (Qiagen) for RNA extraction ...
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bioRxiv - Physiology 2020Quote: ... RNA from human islets (∼150 for each donor) was extracted with RNeasy Mini Kit (Qiagen) and was reverse transcribed using the High Capacity Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from 150-200 islets using the RNeasy Micro kit (Qiagen, Valencia, CA). RNA was quantified by spectrophotometry with a NanoDrop 2000 (Life Technologies Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments <150 bp in length were removed using the GeneRead Size Selection Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: PCR products were purified with the QIAquick PCR purification kit (Qiagen) and submitted to Genewiz for sequencing.
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified (MinElute PCR purification kit, Qiagen, 28004) and 4 μL was mixed with 1 μL Salt solution (1.2M NaCl ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified using QIAquick PCR Purifcation Kit (Qiagen, Germany). Illumina adaptors were ligated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... The final PCR libraries were purified on PCR purification columns (QIAGEN), and the eluted DNA was mixed at a ratio of 1:5:5 from the low ...
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bioRxiv - Genomics 2020Quote: ... PCR products were purified using a QIAquick PCR Purification Kit (Qiagen) and then sequenced by Sanger platform.
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bioRxiv - Molecular Biology 2021Quote: ... The final PCR libraries were purified on PCR purification columns (QIAGEN), and the eluted DNA was mixed at a ratio of 1:5:5 from the low ...
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bioRxiv - Microbiology 2019Quote: ... Real-time PCR mix included 0.5X Quantifast Multiplex PCR MasterMix (QIAGEN), 400 mM of each forward and reverse primer ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified using MinElute PCR purification kit (Qiagen) and used as dsDNA donors later ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The PCR product was cleaned using QIAquick PCR Purification Kit (Qiagen), 3’A-tailed using Taq polymerase (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... All PCR products were purified with QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... PCR products were purified with QIAquick PCR purification kits (QIAGEN, Germany), mixed with primers ...
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bioRxiv - Genetics 2020Quote: ... PCR products were purified using the QIAquick PCR purification kit (QIAGEN) and diluted to 20 ng/µL ...
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bioRxiv - Pathology 2019Quote: ... PCR products were cleaned using the QIAquick PCR Cleanup kit (Qiagen) following manufacturer’s instruction followed gel electrophoresis using 1 kb ladder (Promega) ...
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bioRxiv - Genetics 2019Quote: ... PCR products were purified using the Qiaquick PCR purification kit (Qiagen), all 18 capsids (for the 18 parent library ...