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Citations for Qiagen :
1551 - 1600 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Liver tissue (50-70 mg) was homogenized in 500 μl of 0.1 % NP-40 in PBS using a bead mill Tissue Lyser (Qiagen) and centrifuged for 2 min at 3000 x g to remove the insoluble material ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the sediment samples (∼500 mg wet weight) with the PowerSoil DNA bead tubes (Qiagen, USA), using the FastPrep-24™ Classic (MP Biomedicals ...
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bioRxiv - Molecular Biology 2020Quote: ... Frozen samples were homogenized with zirconia beads YTZ-4 (AS-ONE, Osaka, Japan) using TissueLyser II (Qiagen, Hilden, Germany), and total RNA was then extracted using the Maxwell 16 LEV Plant RNA Kit (Promega ...
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bioRxiv - Immunology 2021Quote: ... Samples were added to 2 ml ZR BashingBead lysis tube with 0.5 mm beads (Zymoresearch) and tubes arranged in a pre-cooled Tissuelyzer II adapter (Qiagen) and disrupted by shaking at 50 Hz for 25 min ...
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bioRxiv - Microbiology 2021Quote: ... Sample tubes were transferred back to ice before 3 rounds of 60 second bead beating on the TissueLyser2 (Qiagen). Samples were incubated at room temperature for five minutes before 200 µL of chloroform (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed to a fresh 50 mL conical and further incubated with 2mL of Talon Beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Amplified libraries were then purified using 1.5x ratio of SPRI beads to remove adapter dimers and eluted in 50 μl of EB (Qiagen) after an incubation for 10 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were equalized and His6-HA-SUMO1-conjugates were enriched on nickel-nitrilotriacetic acid (NiNTA) agarose beads (Qiagen, #L30210) as described in15 ...
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bioRxiv - Neuroscience 2022Quote: ... After the third wash the beads were slowly resuspended in 350µL RLT buffer (from Qiagen RNeasy Mini kit #74004) by slowly pipetting up and down and incubated on a rotating nutator for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... The tissue samples were disrupted and homogenized by adding 2 stainless steel beads (SSB 32) using the TissueLyserII (Qiagen) at 20 Hz for 5 mins ...
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bioRxiv - Microbiology 2022Quote: ... homogenized in 500 μl PBS by bead beating (3 mm steel ball, 25 Hz for 2.5 min in a TissueLyser (Qiagen)) ...
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bioRxiv - Pathology 2023Quote: Sixty µl of blood was transferred to a 1.4 mm ceramic beads pre-filled tube containing 600 µL of RLT from the RNeasy Mini Kit (Qiagen), samples were homogenized ...
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bioRxiv - Molecular Biology 2022Quote: ... The final PCR product was purified once more with 1,45x AMPure XP beads and eluted in 20 µL of the Buffer EB (QIAGEN). The libraries were quantified using the KAPA Library quantification kit (Kapa Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 14-666-315) containing 1 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Nucleic acids were isolated from excised granulomas by homogenizing in TRIzol (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in tubes containing glass beads (200 mg, 0.4 mm) and were lysed using a TissueLyser LT (Qiagen). To minimize protein degradation ...
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bioRxiv - Microbiology 2023Quote: ... 25 mg of freeze-dried leaf tissue was ground into a fine powder using two 1.5 mm diameter steel beads in a TissueLyser II (Qiagen) at 30 Hz for 1 minute ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... The kidneys were homogenized using 0.5 M acetic acid and two 5-mm steel beads in TissueLyser LT (Qiagen), followed by addition of pepsin to 0.1 mg/ml and incubation for 3 days ...
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bioRxiv - Microbiology 2023Quote: ... and seeds (in situ) were ground using two sterile stainless-steel beads in a Tissue Lyzer II (Qiagen, Germany) for 3×30s at 30Hz ...
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bioRxiv - Microbiology 2023Quote: ... The clarified supernatant was passed through the pre-equilibriated nickel-nitrilotriacetic acid (Ni-NTA) beads (Qiagen, Germantown, MD, USA) in a gravity flow column ...
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bioRxiv - Immunology 2023Quote: ... pH 6.0) and homogenized using a sterile 5mm stainless steel bead in a Tissue Lyser 2 (Qiagen, Hilden, Germany) for 90 seconds at 1/60Hz ...
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bioRxiv - Microbiology 2023Quote: ... Cells suspended in lysis buffer were transferred to a 2 mL screw cap tube that contained 0.5 mL of 0.1 mm disruption beads for bacteria and 0.7 mL QIAzol™ Lysis Reagent (Qiagen; 79306). Cells were lysed via mechanical disruption using a bead beater for four rounds of 30 s each ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was 0.2 µM filtered and was incubated overnight at 4°C with 5 mL of Ni-NTA beads (Qiagen). XXX
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the supernatant incubated 1 hr at 4°C with 1.5 ml packed Ni-NTA Agarose beads (Qiagen). Beads were washed with buffer H adjusted to 50 mM imidazole ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amplified libraries were then purified using 1.8x ratio of MagBio beads to remove adaptor dimers and eluted in 30 μL of EBT (QIAGEN) after an incubation for 5 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg⋅ml –1 Leupeptin and 3 mM Benzamidine) with steel beads at 28.5 Hz for 1 min (Qiagen TissueLyser II ...
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bioRxiv - Microbiology 2023Quote: ... containing a sterile 4.5mm ball bearing and homogenized with 2 rounds of bead-beating (2m30s, 30 beats/s) in a TissueLyserII (Qiagen). The lysate was then transferred to a new tube and 1/5 volume of chloroform (Avantor ...
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bioRxiv - Biochemistry 2023Quote: ... the bead slurry was spun down and resuspended in 100 µl wash buffer and digested with proteinase K (Qiagen) at 50 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA and RNA from healthy tissues and lymphomas of control and STIL-transgenic mice was extracted using the All Prep DNA/RNA/Protein Mini Kit according to manufacturer’s instructions after tissue homogenization using TissueLyser II and stainless-steel beads (5 mm, all Qiagen). DNA and RNA concentrations were quantified with Qubit 2.0 using the dsDNA High Sensitivity and RNA High Sensitivity Kit (both Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNAs were purified using 0.8x VAHTSTM DNA Clean Beads and then amplified with Hot Start DNA Polymerase (QIAGEN). The PCR reaction protocol was ...
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bioRxiv - Microbiology 2024Quote: ... The feces were homogenized by bead beating and viral RNA was extracted using the RNeasy Mini QIAcube kit (Qiagen). The tissues were homogenized by bead beating and viral RNA was extracted using the RxnDirect-zol RNA Miniprep Plus kit (Zymo) ...
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bioRxiv - Neuroscience 2024Quote: ... was added to tissue and lysed using a sterile metal bead with rapid agitation in a TissueLyser II (Qiagen). Lysate was then mixed with UltraPure phenol:chloroform:isoamyl alcohol (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 cells were harvested by centrifugation and genomic DNA was prepared using Blood & Cell Culture DNA Midi Kit 100/G Genomic-tips (Qiagen #13343). To examine alkaline degradation 3 μg of DNA was treated with 0.3 M NaOH at 55 °C for 2 hr in 15 μl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... (2015) which is a modified CetylTrimethylAmmoniumBromide (CTAB) protocol (Doyle and Doyle, 1990) combined with a MagAttract suspension G (QIAGEN, Hilden, Germany) purification step (Xin and Chen ...
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bioRxiv - Genomics 2019Quote: ... 200 μl lysis buffer were used per organoid for homogenization at 12,000 x g for 2 min in a QIAshredder Column (Qiagen, Hilden, Germany) after lysis and prior to addition of 70% ethanol ...
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bioRxiv - Genomics 2020Quote: ... ~20 ng genomic DNA was amplified using a whole-genome amplification (WGA) kit according to the manufacturer’s instructions (Qiagen REPLI-g Mini Kit, Qiagen, Valencia, CA). The REPLI-g Kit is developed based on multiple displacement amplification (MDA) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then harvested by centrifugation (5 min, 10,000 × g) and immediately suspended in 1 mL bacterial RNA protect (Qiagen, Germantown, MD), followed by 5 min incubation at room temperature ...
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bioRxiv - Genetics 2020Quote: ... We purified the digested vector from a 0.8% agarose gel (1.2 g agarose Fisher #21-255-00GM) in 150 mL 1x TAE (Fisher #FERB49)) using a QiaQuick Gel Extraction Kit (Qiagen #28706) and quantified using a Qubit dsDNA HS Assay Kit.
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bioRxiv - Neuroscience 2020Quote: ... Whole genome amplification (WGA) was performed directly following FACS using the REPLI-g Advanced DNA Single Cell kit (Qiagen Cat#: 150365) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected by centrifugation (5 min at 500 x g) and RNA was isolated using RNeasy Mini Kit (RNeasy Mini Kit, Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA for sequencing was isolated with the the Qiagen 100/G kit according to the manufacturer’s description (Qiagen, Hilden, Germany) and library preparation and sequencing was done as described previously using Illumina Miseq sequencing (Illumina ...
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bioRxiv - Microbiology 2020Quote: Total bacterial DNA from approximately 0.25 g of colon contents was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions as described13 ...
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bioRxiv - Microbiology 2021Quote: ... Suspended fecal samples and swab samples were centrifuged at 4,000 x g for 15 min and viral RNA was extracted from each supernatant using the QIAamp Viral RNA Mini Kit (Qiagen, USA). Conventional RT-PCR was performed using the Invitrogen™ SuperScript™ IV First-Strand Synthesis System (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were centrifuged for 10 minutes at 4,300 x g and the cell pellet resuspended in the lysis buffer (RLT) from the RNeasy kit (Qiagen, Germantown, MD). Cells were then homogenized with sterile 0.1 mm glass beads (Biospec Products Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... or CRTC3 or control sgRNA (Supplementary Table 2) were transfected into 293FT cells together with packaging plasmids pMD2.G and pSPAX2 using Effectene transfection reagent (Qiagen #301425). The viral supernatants were collected at 48 ...
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bioRxiv - Cell Biology 2021Quote: Cells debris was pelleted by centrifugation at 23,000 x g for 45 minutes and the supernatant loaded onto a 10 ml Ni2+-NTA column (Qiagen, Hilden, Germany). The column was washed with 600 ml buffer A and protein batch eluted with 100 ml buffer B (buffer B = buffer A supplemented with 290 mM imidazole).
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bioRxiv - Immunology 2022Quote: ... The lysate was then clarified by centrifugation at 16,000 x g for 45 minutes and then applied to 3 mL Ni-NTA resin (Qiagen, Valencia, CA) equilibrated with buffer A ...
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bioRxiv - Microbiology 2020Quote: ... total DNA from the sample (20 g) was extracted using the Qiagen DNeasy® PowerSoil® Pro Kit (Qiagen, Hilden, Germany) and the integrity of DNA was evaluated by gel electrophoresis ...
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bioRxiv - Neuroscience 2022Quote: 1 µg of RNA was used for cDNA synthesis using the QuantiTect Reverse Transcription Kit according to the manufacturers’ protocol (Qiagen 205313). Quantitative PCR was carried out on a RotorGeneQ (Qiagen ...