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Citations for Qiagen :
1451 - 1500 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were then incubated at 4 °C for 1-1.5 hours on one ml of Ni-NTA resin (Qiagen). The resin was then loaded into columns (Biorad ...
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bioRxiv - Molecular Biology 2019Quote: ... and the supernatant was added to Ni-NTA agarose resin (0.5 mL bed volume per liter of cell culture; Qiagen) equilibrated in Ni-NTA lysis buffer and agitated by rocking at 4°C for 60 min ...
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bioRxiv - Immunology 2021Quote: Bacterial plasmid DNA was extracted from a 3 ml overnight culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Next ...
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bioRxiv - Neuroscience 2020Quote: ... each individual cell was transferred to a 0.2 ml PCR tube containing 2 μl of lysis buffer (RLT Plus - Qiagen). The tube was immediately placed on a metal plate sitting on top of dry ice for flash-freezing ...
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bioRxiv - Bioengineering 2021Quote: ... The complex (12 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Neuroscience 2021Quote: ... Every second cryosections (35 μm thick) was collected into 1.5 mL eppendorf tubes containing 350 μl RLT Plus Buffer (Qiagen) supplemented with 1% 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 μg of mHTTex1 seed or 50 μg of seeded neuronal lysate was incubated with varying doses of proteinase K (0 to 0.5 μg/mL, Qiagen) for 30 min at 25°C and heat inactivated at 75°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... A total of OD600 = 0.3 in 1 ml of cells was pelleted and gDNA was extracted using DNeasy Blood & Tissue kit (Qiagen) using the Gram-negative bacterial protocol followed by quantification using Qubit ...
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bioRxiv - Microbiology 2020Quote: ... The complex (7.5 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mg of freeze-dried and ground roots were mixed with 1.6 mL of 1 M perchloric acid and homogenized in a TissueLyser II (Qiagen) in microtubes containing 6 glass beads of 2.8 mm in diameter ...
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bioRxiv - Microbiology 2020Quote: ... and midguts were individually transferred to 1.5 ml screw-top tubes with 350 μl RLT Plus buffer from Qiagen AllPrep DNA/RNA Mini Kit and kept at −20°C until DNA and RNA extraction.
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bioRxiv - Microbiology 2020Quote: Viral RNA was purified from 140 mL of cell culture supernatant using the QIAamp Viral RNA Mini Kit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue samples were homogenized in 3 mL sterile PBS for 20 seconds on ice using a TissueRuptor homogenizer (Qiagen) with sterile tips ...
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bioRxiv - Bioengineering 2022Quote: ... protein yields of the CNA35 probe were synthesized using E.Coli bacteria before purification using ÄKTApurifier (Cytiva) and a 5 ml Ni-NTA Superflow Cartridge (Qiagen), dialysis with SnakeSkinTM Dialysis tubing with 10 kDa MWCO ...
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bioRxiv - Systems Biology 2022Quote: ... Ten millilitres of culture were pelleted by immediate centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was washed three times with ice-cold PBS and suspended in 1 ml TRIzol™ reagent (QIAGEN). Instead ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining recovery was grown overnight in 150 mL LB + chloramphenicol and plasmid DNA was isolated by MaxiPrep (Qiagen). The complexity of the SpCas9 catalytic and PAM domain libraries were estimated to be 77,400 and 292,600 respectively ...
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bioRxiv - Bioengineering 2022Quote: Nuclei were isolated from differentiating white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 uL of Tris-Hydrochloride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: Tissue-gel samples were equilibrated in DNase buffer (750 ml) for 30 min and then incubated with RNase-free DNase1 (2.7 Kunitz units/µl, 50 µl) (Qiagen) in DNase buffer (450 µl ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled ORC were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled Cdt1 were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer D with 10 mM imidazole for 1.5 hr with rotation at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing SctV9 without higher order aggregates of SctV were selected and incubated with 1.5 mL of Ni2+- NTA resin (Qiagen) pre-equilibrated in Buffer F (6 h ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 20 min under constant rotation with 3,5 ml of pre-washed Ni-NTA superflow beads (Qiagen). Beads were collected by centrifugation ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Microbiology 2022Quote: Pellets from 50 ml culture were processed for RNA extraction using the RNeasy PowerSoil Total RNA Kit (Qiagen, USA). A DNase kit (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The lysates were cleared by centrifugation at 10,000 xg for 30 min and added to 1 ml of Ni-NTA agarose (Qiagen) pre-equilibrated with the same lysis buffer ...
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bioRxiv - Microbiology 2019Quote: A total of 250 ng of Qst was incubated with 1 ml Ni-NTA Superflow beads (Qiagen, Hilden, DE) in an end-over-end shaker for 1 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant (enriched with SGs) was transferred to a new 1.5 ml microcentrifuge tube and purified using the RNeasy Plant Mini Kit (QIAGEN).
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bioRxiv - Microbiology 2019Quote: ... or lungs (pneumonia) were harvested and homogenized in 1 mL PBS + 0.1% Triton-X100 using a TissueLyser LT (Qiagen) before plating to LB agar + 5 μg/ml irgasan to enumerate bacterial burden.
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bioRxiv - Immunology 2020Quote: ... or IFNγ (10ng/mL) for 6 hours prior to harvesting and RNA extraction using RNeasy Mini Kit (Qiagen 74106). Next ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein was normalized to 2 mg across all samples tested and incubated with 0.25 mL of 50% Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was isolated in six biological replicates per strain from 0.5 mL of culture using the RNeasy Protect Bacteria Mini kit (Qiagen) on six consecutive days ...
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bioRxiv - Developmental Biology 2020Quote: ... Femoral vein blood collection (1.0-2.0 mL) was performed on each animal and blood was placed in to PAXgene blood collection tubes (Qiagen). Whole blood was frozen and stored at −80° C for shipment to UCLA for methylation analysis.
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bioRxiv - Bioengineering 2021Quote: Nuclei were isolated from white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 ul of Tris-Hydrochloride (Sigma ...
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bioRxiv - Genetics 2021Quote: ... 10 mM imidazole (final concentration) was added to the sample and the solution was bound to 0.5 ml NiNTA resin (Qiagen). The resin was washed with NTA buffer A1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... cells were first washed and pelleted five times with 15 ml of PBS then lysed with QIAzol reagent (Qiagen), RNA was extracted according to manufactures directions ...
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bioRxiv - Immunology 2020Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen No. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were centrifuged for 30 min at 45,000 × g and incubated with 1 ml of Ni-NTA agarose (Qiagen) per 1 l expression culture ...
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bioRxiv - Biochemistry 2021Quote: ... The ankyrin repeat domains were purified over a Ni-nitrilotriacetic acid column (2.5 ml column volume) according to the manufacturer’s instructions (QIAgen, Germany). Up to 200 mg of highly soluble ankyrin repeat domains were purified from one liter of E ...
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bioRxiv - Genetics 2021Quote: ... We extracted DNA from approximately 1-2 million cells of actively growing culture by first pelleting the cells and resuspending them in 1.0 mL Cell Lysis Solution (Qiagen). The samples were incubated with RNase A solution at 37°C for 40 min ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen pieces of tissue (~20 mg) were homogenized in 0.5 ml TRIzol in a TissueLyser II (Qiagen, Hilden, Germany) (2 × 2 min at 30 Hz ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...