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Citations for Qiagen :
1701 - 1750 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The libraries were purified with a 30-minute incubation at 37 °C in the presence of 160 μg/mL RNase A (Qiagen), a treatment with 200 μg/mL Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... The homogenate was treated overnight with proteinase K (2 mg/ mL) at 56 °C and DNA was extracted using the MagAttract PowerSoil DNA EP Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: 7-day old in vitro grown plantlets or adult leaves of soil grown 3-4 week-old plants were ground in 2 mL tubes using a Tissue Lyser (Qiagen) twice for 30 sec at 30 Hz before RNA extraction using the RNeasy Plant Mini kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... a ∼20 mg chunk of frozen lung tissue was collected using a scalpel and placed in 1 mL RLT buffer (Qiagen) with 1:100 β-mercaptoethanol in a Precellys silicone bead dissociation tube (Bertin-Corp) ...
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bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease40 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Physiology 2023Quote: ... were immersed in the Transnetyx microbiome sample collection tube (1.5 mL, prefilled with DNA stabilization liquid and glass beads) and sent to Transnetyx (Cordova, TN) for automated DNA extraction (Qiagen DNeasy 96 PowerSoil Pro QIAcube HT extraction kit) ...
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bioRxiv - Cancer Biology 2023Quote: Investigation of the cell cycle related genes in LoVo cell line on treatment with FA FOS I was performed as described above by using the synchronized and 24 h FA FOS I (198 µg/ml) treated cells profiled by using RT2 Prolifer PCR Array- Human Cell cycle from Qiagen. The data was analysed by using Gene globe platform and reactome pathway analysis.
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bioRxiv - Microbiology 2022Quote: ... Bacterial cells were harvested from the surface of the cheese agar by using a sterile razor blade and were then immediately placed into 3 mL of RNAProtect Bacteria Reagent (Qiagen) and frozen at -80C until RNA extraction ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1.5 h in 50 mL conical tubes ...
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bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with ice-cold PBS and lysed at room temperature with 1 ml/well QIAzol lysis reagent (QIAGEN). Lysates were incubated at room temperature for 5 min and homogenised by pipetting up and down ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were selected in puromycin (1 μg/ml) the next day and after 4 days genomic DNA was extracted using DNeasy Blood and Tissue kit (Qiagen). Genomic DNA was sonicated to an average size of 500 bp using S2 Focused-ultrasonicator (Covaris ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants were then supplemented with 20 mM imidazole and then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) that were pre-washed with 5 column volumes of 1x PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Physiology 2023Quote: ... The digest was run on an 1% Agarose gel (TAE buffer, 0.25 µg/ml EtBr) and the the QIAquick Gel extraction kit (Qiagen, 28704) was used to extract the pLV6 backbone according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1 h in 50 mL conical tubes ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from approximately 3 ml of whole blood using the Gentra Puregene Blood Kits (#158467; Qiagen, Hilden, Germany), following the “Whole Blood” subsection in the manufacturer-provided handbook ...
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bioRxiv - Microbiology 2023Quote: ... was added to the supernatant to give a final concentration of 1 mM and the resultant suspension was applied to a 1 ml Ni2+-NTA column (Qiagen). The resin was then washed sequentially with 10 ml of lysis buffer (without Triton X-100) ...
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bioRxiv - Genomics 2023Quote: ... tubes were incubated at 56°C for 1 hour followed by addition of 2ul of RNAse A (100mg/ml, QIAGEN) to 1ml of saliva sample and incubated at room temperature for 5 minutes ...
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bioRxiv - Genetics 2023Quote: ... The leaves were ground in liquid nitrogen and powder was resuspended in 2 ml of RLT buffer from RNeasy Plant Mini kit (Qiagen). For each sample ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged for 30 min at 38,000 g and 4°C and cleared supernatants loaded onto Ni-NTA columns with 0.5 mL bed volume (1018244 Qiagen, Germany). The columns were washed sequentially with 3 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: ... RpaB was purified following the same protocol39 using Precellys 24 (Bertin Technologies) to disrupt the cells and 0.5 mL of bedded Ni-NTA agarose beads (Qiagen GmbH) to bind 6×His-tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting DNA pellets were washed with 1.0 mL 70% ice-cold ethanol and re-suspended in 30 µL of EB buffer (Qiagen, Germany) after drying.
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bioRxiv - Molecular Biology 2023Quote: ... The BmAgo3 immunoprecipitated beads were divided into two and incubated in buffer D with/without 200 μg/ml RNase A (Qiagen) at 30°C for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... about 100 mg of tissue samples were homogenised in 1 mL of TRI reagent at room temperature using a tissue lyser (Qiagen). The homogenised samples were transferred to fresh microcentrifuge tubes for RNA isolation ...
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bioRxiv - Biochemistry 2023Quote: ... HBEC5i cells with treated with either non-specific siRNA or Flvcr2 siRNA were harvested and kept in 1 mL TRI reagent (Qiagen).
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bioRxiv - Bioengineering 2023Quote: ... the cells were pelleted at 15000 g at 4 °C for 30 minutes and the supernatant was supplemented with 20 mM of imidazole before loading onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Bioengineering 2023Quote: ... Lysate supplemented with 20 mM imidazole was cleared by centrifugation at 15000 g at 4 °C for 30 minutes and the supernatant was then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C with agitation for six hours after which time 1 mL cell pellets were treated with RNAprotect (Qiagen). Cell pellets were washed in 0.5 mL PBS pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... preceded by a 10-minute bead-beating step at 30 Hz in 2 ml e-matrix tubes (MP Biomedical, USA) using a Tissuelyser II (Qiagen). Molarity and fragment-length distribution of the extracts were measured using a Tapestation ...
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bioRxiv - Immunology 2023Quote: ... A 0.3 ml portion of this homogenate was used to extract and purify RNA using the RNeasy Mini Kit (CAS: 74104, Qiagen) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples and inputs were incubated for 4 hr at 37°C with 1.5 µl of 10 mg/ml RNase A (Qiagen, 1007885) and 15 µl 10% sodium dodecyl sulfate and 3.5 µl 20 mg/ml Proteinase K (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... resuspended in 50 ml of distilled water and the protocol was modified to include 0.1 mm glass beads (Qiagen, Germany) to increase DNA extraction by mechanical lysis ...
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bioRxiv - Microbiology 2024Quote: ... 10 Arabidopsis seedlings from the same MS plate were sampled together in 2 mL microtubes containing two 3 mm-diameter tungsten carbide beads (Qiagen), and flash frozen in liquid nitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The bits containing the powder was immediately transferred and rinsed in a 2 ml Eppendorf (DNA/RNA and DNase/RNase free tube) containing 1 ml of Tissue Lysis buffer ATL from the QIAamp DNA Micro Kit (Qiagen) and stored at 4°C until the DNA extraction process (i.e ...
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bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Synthetic Biology 2023Quote: The pARP3 plasmid for methylation analysis was extracted from 200 mL of an overnight culture of Tol 5 transformant cells using the HiSpeed Plasmid Midi kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from five 10-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from ten 4-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
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bioRxiv - Systems Biology 2024Quote: ... and left brain hemisphere using the phenol-chloroform extraction method with 1 mL of Qiazol Lysis Reagent (Qiagen, Hilden, Germany), as previously described in Ederer et al ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
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bioRxiv - Biophysics 2023Quote: ... The soluble extracts were applied to 2 ml columns of nickel-nitrilotriacetic acid agarose (Ni-NTA) (QIAGEN catalogue no. 30210) that had been equilibrated with lysis buffer ...