Labshake search
Citations for Qiagen :
1301 - 1350 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: Total RNA was extracted from 2 g of rhizosphere soil using the RNeasy PowerSoil Total RNA Kit (QIAGEN, Canada) and 0.5 g roots using RNeasy Plant Mini Kit (QIAGEN ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4.5 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1 h in 50 mL conical tubes ...
-
bioRxiv - Physiology 2023Quote: DNA extraction was performed from 0.1 g of fecal sample using QIAamp PowerFecal Pro DNA Kit (Qiagen, Hilden, Germany). The solid fecal sample was added into PowerFecal Pro tubes containing pre-filled beads and the extraction was performed according to the manufacturer’s instruction with additional bead-beating step (ensruing a total of three bead-beating steps ...
-
bioRxiv - Microbiology 2023Quote: ... The solubilized fraction was centrifuged (30 min, 100,000 x g) and the supernatant was purified via Ni-NTA agarose columns (Qiagen) and eluted with a discontinuous imidazole gradient using an ÄktaGo system ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (100,000 x g for 30min) and then bound to Ni-NTA Agarose (Qiagen) for 1h at 4°C with shaking ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by centrifugation at 20,000 x g for 30 min and loaded on a 5 ml Ni-NTA Superflow column (QIAGEN) followed by extensive washing ...
-
bioRxiv - Neuroscience 2023Quote: ... Whole genome amplification (WGA) was performed directly following FACS using the REPLI-g Advanced DNA Single Cell Kit (Qiagen) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: Ten millilitres of culture were pelleted by centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
-
bioRxiv - Genomics 2024Quote: ... each 50 mL midiprep culture was spun down for 30 minutes at 4,300 x g and plasmid DNA isolated using a midiprep kit according to the manufacturers instructions (Qiagen).
-
bioRxiv - Bioengineering 2023Quote: ... purified genomic DNA (25 ng) was first amplified according to the manufacturer’s instructions using REPLI-g Single Cell kit (Qiagen). Amplified genomic DNA was then diluted into ∼800 ng/μl and 1 μl DNA was used for each qPCR reaction ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The lysate was centrifuged at 50,000×g for 10 min and to the supernatant 1 mL of 50% Ni−NTA (Qiagen) was added and incubated for 1 h at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer in a 50 mL falcon tube ...
-
bioRxiv - Bioengineering 2024Quote: ... spun down for 15 s at 8,000 g and eluted 3 times by adding 700 µL Buffer RW1 (Qiagen) and spinning ...
-
bioRxiv - Bioengineering 2024Quote: ... A subset of the samples (5 g) was preserved in 5 mL RNA preservation solution (RNAprotect, QIAGEN, Hilden, Germany) and stored at -80°C ...
-
bioRxiv - Cell Biology 2024Quote: From pellets of 100 million cells genomic (g)DNA was extracted using the QIAamp DNA Blood Maxiprep Kit (Qiagen) according to manufacturer’s instructions with the following modifications ...
-
bioRxiv - Microbiology 2024Quote: ... Lysates were clarified by centrifugation at 21,000× g at 4°C and loaded onto gravity flow Ni-NTA agarose columns (Qiagen), followed by washing with 50 mM HEPES pH 7.5 ...
-
bioRxiv - Genetics 2021Quote: ... Biotinylated RNA was released from beads using 100 mM DTT and cleaned using RNeasy MinElute Kit (QIAGEN 74204). Half of eluted RNA or 600 ng of depleted RNA was ligated to 5 µM of 50 adenylated ...
-
bioRxiv - Cancer Biology 2021Quote: Mouse-derived xenograft HCC tumors were homogenized in RIPA buffer using metal beads and the LT TissueLyser (Qiagen). Samples were sonicated (Branson Sonifier 3310s ultrasound probe ...
-
bioRxiv - Developmental Biology 2021Quote: ... all remaining bead-bound EGFP+ nuclei were processed for RNA extraction using the RNeasy Plus Micro kit (Qiagen). Nuclear RNA-seq libraries were constructed with the Stranded RNA-seq Kit with Ribo Erase (Kapa Biosystems ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... two tubes of each strain were freeze-dried overnight and macerated using a TissueLyzer II bead-beater (Qiagen). Two 2 mm metal beads were placed in each tube ...
-
bioRxiv - Microbiology 2022Quote: ... beat-beading tubes were filled 1/3 with beads and soaked overnight in 500 μl buffer RLT (Qiagen). Tubes were spun at full speed and excess buffer removed ...
-
bioRxiv - Neuroscience 2019Quote: ... The tissue–methanol mixture was subjected to bead-beating for 45 sec using a Tissuelyser cell disrupter (Qiagen). Extracts were centrifuged for 5 min at 5,000 rpm to pellet insoluble material and supernatants were transferred to clean tubes ...
-
bioRxiv - Genomics 2019Quote: ... beads were washed with native lysis buffer three times followed by RNA extraction using RNeasy mini kit (Qiagen). 12.5μl RNA was used for cDNA synthesis with Advantage RT-PCR kit (Clontech) ...
-
bioRxiv - Biochemistry 2020Quote: ... RNA was eluted from the beads with RLT buffer and purified using RNEasy MinElute columns (Qiagen, catalog #74204). RNA was eluted into 10 μL of nuclease free water and analyzed on Agilent 2100 Bioanalyzer using the RNA 6000 Pico kit (Agilent ...
-
bioRxiv - Microbiology 2021Quote: ... samples were crushed with a mortar and pestle and further homogenized with glass beads in a TissueLyzer (Qiagen). Total RNA was extracted using phenol/chlorophorm/isoamylalcohol phase-separation followed by a GeneJET RNA Purification Kit (Thermo Scientific™) ...
-
bioRxiv - Physiology 2021Quote: Liver homogenates were prepared in cold conditions (+4°C) by adding a stainless-steel bead (Qiagen, cat. 69989) and tissue lysis buffer (composition ...
-
bioRxiv - Plant Biology 2021Quote: ... small pieces of leaf tissue were flash frozen in liquid nitrogen and ground in a bead mill (Qiagen). Tissue powder was incubated in extraction buffer (100 mM Tris-HCl pH9.5 ...
-
bioRxiv - Genomics 2021Quote: DNA from fecal samples was isolated using the MagAttract PowerMicrobiome DNA/RNA kit with glass beads (Qiagen, Germany). Metagenomic libraries were prepared using the NEBNext Ultra II for DNA Library Prep kit (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... 6xHIS-SUMO2 conjugates were purified under denaturing conditions using Ni-NTA-agarose beads according to manufacturer’s instructions (Qiagen). Briefly ...
-
The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was split between two columns each with 2 ml Ni-NTA agarose beads (Qiagen, 30210) pre-equilibrated with Lysis Buffer ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Samples were then ground to powder using a 3 mm tungsten carbide bead (Qiagen Cat. No. / ID: 69997) on a TissueLyser II (Qiagen ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Homogenization of the samples was performed using a Retsch bead beater (Retsch GmbH) in RLT plus buffer (Qiagen). RNA was enriched using a PolyA enrichment protocol implemented in the TruSeq RNA library preparation kit ...
-
bioRxiv - Biochemistry 2020Quote: ... homogenized by cryomilling after addition of a 2.5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 30 seconds 3–6 times ...
-
bioRxiv - Microbiology 2021Quote: ... 500 µl of PBS-AB was added to each sample along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized during 10 min and then centrifuged at 15,000 g for 10 min ...
-
bioRxiv - Systems Biology 2022Quote: ... Supernatants were collected and purified with Ni2+ Sepharose beads (Smart-lifesciences, China) or Ni-NTA Agarose (QIAGEN, Germany), then washed with lysis buffer (For the purification of biotinylated protein ...
-
bioRxiv - Plant Biology 2020Quote: ... The clear supernatant was then gently mixed with 1 ml of Ni+2-NTA agarose beads (Qiagen, Germany) for 30 min and then added to the column with a flow rate of 0.5 ml/min ...
-
bioRxiv - Microbiology 2019Quote: ... The final concentrated solution was transferred to a microtube containing Pathogen lysis tube L beads (Qiagen, Hilden, Germany), 50 μl of Solution SL and 200 μl of sterile saline solution to homogenize ...
-
bioRxiv - Developmental Biology 2020Quote: ... benthamiana leaves were grinded in a tube with two glass beads (3 mm) with a TissueLyser II (Qiagen) and directly after supplied with 600 μl EB ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The m6A-enriched fragmented RNA was eluted from the beads and purified using the RNeasy mini kit (Qiagen). The quality of the m6A-enriched RNA was assessed with the Bioanalyzer ...
-
bioRxiv - Microbiology 2021Quote: ... for 30 minutes at 37°C followed by beadbeating with 50μg 0.1mm zirconium beads for 6 minutes on the Tissuelyzer II (Qiagen) prior to loading onto the Qiacube HT ...
-
bioRxiv - Cell Biology 2019Quote: ... and placed into 2ml eppendorf tubes containing 200μl LB and a stainless steel bead of 5mm diameter (Qiagen). Flies were homogenized using the TissueLyser II (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: ... We then washed beads four times in 1 ml of Modified RLT buffer (RLT buffer supplied by Qiagen with added 10 mM Tris pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... lumbar spinal cord or the glabrous skin of the footpad were harvested and homogenized in 0.1M HCl + 0.5% triton X-100 using glass beads and a TissueLyser II (Qiagen). Lysate was centrifuged at 18,000 xg and 4 °C for 40 – 60 min to pellet cell debris ...
-
bioRxiv - Cancer Biology 2021Quote: ... Xenograft and HCC mouse samples were homogenized in RIPA buffer using metal beads and the LT tissuelyzer (Qiagen). All samples were sonicated (ultrasound probe ...
-
Bioaugmented sand filter columns provide stable removal of pesticide residue from membrane retentatebioRxiv - Microbiology 2020Quote: ... The filters were then transferred to bead tubes supplied with the DNeasy PowerWater Kit (Qiagen, København Ø, DK) and frozen for subsequent DNA extraction according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... 500 µL of PBS-AB was added to each sample (Lungs = 0.01 – 0.04g; Cecum = 0.3 -0.5g) along with Tungsten carbide 3mm beads (Qiagen, Gaithersburg, MD). Samples were homogenized at a speed of 10 Hz for 10 min ...
-
bioRxiv - Genomics 2020Quote: ... PCR products were cleaned-up using 1:1 of SPRI beads and eluted in 30µl elution buffer (Qiagen). The resulting amplicons were assayed on the Fragment Analyzer System (Agilent) ...
-
bioRxiv - Biochemistry 2022Quote: ... coli as described in (Péli-Gulli et al., 2015) using Ni-charged agarose beads (QIAGEN, product number 30210). Proteins were eluted in elution buffer (50 mM NaH2PO4 pH 8.0 ...
-
bioRxiv - Cell Biology 2022Quote: ... and the soluble fraction of the lysate was incubated with Ni2+-nitrilotriacetic acid (NTA) beads (Qiagen, Hilden, Germany). Proteins were eluted with 300 mM imidazole in 60 mM NaH2PO4 ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA (gDNA) of fecal microbe were extracted using a silica bead beating-based protocol adapted from Qiagen MagAttract PowerMicrobiome DNA/RNA Kit [Qiagen 27500-4-EP] ...