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Citations for Qiagen :
1151 - 1200 of 2490 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads as per the manufacturer instructions (Qiagen, Hilden, Germany). Beads were washed ...
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bioRxiv - Biochemistry 2020Quote: ... Nickel affinity purification of 10His-SUMO1T95R conjugates was carried out with Ni-NTA agarose beads (Qiagen). 25μl beads were prepared per 1ml lysate by washing for three times with guanidine lysis buffer and added to the cell lysate for mixing overnight at 4□ ...
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bioRxiv - Genomics 2021Quote: ... Flies were individually disrupted using a 3mm diameter steel bead in a TissueLyser (Qiagen, Germantown, MD) for 30 seconds at 30Hz in 100uL of 2mg/mL Proteinase K in PK buffer (MagMAX™ ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... Muscles were homogenized 1 min at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in 300 μl ice-cold homogenization buffer ...
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bioRxiv - Microbiology 2021Quote: ... following manufacturer’s instructions with two modifications: bead beating was performed in a TissueLyzer (Qiagen, Hilden, Germany) for 10 minutes and autoclaved MilliQ water was used instead of the kit’s buffer in the last elution step ...
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bioRxiv - Molecular Biology 2020Quote: ... the collected eluate was incubated with pre-washed nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen) for 90 min at 4°C to remove the His-tagged TEV protease ...
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bioRxiv - Genomics 2021Quote: ... After physical cell disruption through bead-beating using a Tissuelyser II machine (Qiagen, Cat. No. 85300), DNA extraction was performed using a custom nucleic acid extraction protocol (details explained in (25)) ...
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bioRxiv - Microbiology 2022Quote: ... was added to the tube together with a 5 mm stainless steel bead (Qiagen, Maryland, USA). The sample was homogenized for two minutes at 30 Hz using a TissueLyser II (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... with blinding to treatment groups.12 Bilateral biceps muscles were first bead homogenized (TissueLyser II; Qiagen) at a frequency of 30 Hz for 3 cycles of 2 minutes in lysis buffer [10 mM Tris (pH 7.4) ...
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bioRxiv - Molecular Biology 2022Quote: ... the suspension was centrifuged and the supernatant was loaded onto a Ni-NTA agarose beads (Qiagen) affinity column supplemented with 10 mM imidazole ...
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bioRxiv - Genomics 2022Quote: ... DNA was finally purified using 10 ul of silica beads (QIAEX II Gel Extraction Kit, Qiagen) and eluted in 30 ul elution buffer ...
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bioRxiv - Biochemistry 2022Quote: Dried mycelia and zoospores were ground to a fine powder using beads and Biosprint shaker (Qiagen). 300 µl of extraction buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was supplemented with 30 mM Imidazole-HCl and incubated with Ni-NTA beads (Qiagen), 2 ml of beads for 500 ml culture ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen tissues were placed into tubes containing a 5 mm stainless steel bead (Qiagen, Courtaboeuf, France) and 1 ml of Trizol reagent (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... skin was homogenized in TRIzol using two 7 mm metal beads and a TissueLyser LT (Qiagen). Homogenates were centrifuged to separate an RNA containing aqueous phase ...
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bioRxiv - Microbiology 2019Quote: ... Samples were then homogenised using 5mm steel beads and a Tissue Lyser II (Qiagen, Hilden, Germany) at 15Hz for 20 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... the resolubilized histidine-tagged histones were purified with Ni affinity chromatography using Ni-NTA beads (Qiagen). For untagged H2B ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Subjects transferred collected material directly into the bead tube of a PowerSoil DNA Isolation kit (Qiagen). We extracted DNA following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... sorokiniana were ground with two stainless steel beads (5 mm) using a TissueLyser (Qiagen, Hilden, Germany) at 30 Hz for 1 minute at room temperature and soaked in 1 ml of 100 mM sodium acetate buffer pH 5.0 at 70°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 mM Tris pH 8.0) using 5 mM stainless steel beads in a TissueLyser II (Qiagen). In vitro TPP1 assay was performed as described ...
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bioRxiv - Systems Biology 2019Quote: ... Beating beads were added and the samples were homogenized in 350 µl of cold Qiazol (Qiagen) lysis reagent in a TissueLyser (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... were directly placed in a bead tube of a DNeasy PowerBiofilm Kit (QIAGEN, Germantown, MD, USA) under a laminar flow cabinet ...
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bioRxiv - Microbiology 2021Quote: ... The samples were subjected to bead beating using the Qiagen PowerLyzer 24 Homogenizer (Qiagen, Germantown, MD) at 2500 speed for 45 seconds ...
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bioRxiv - Immunology 2020Quote: ... All samples from bead-based assays were performed using a LiquiChip-200 instrument (Qiagen, Hilden, Germany) using Bio-Plex Manager v6.1 software for quantification.
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bioRxiv - Immunology 2022Quote: ... primers followed by reverse phase 0.65 x SPRI beads purification and a QIAquick Spin Column (QIAGEN) purification ...
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bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
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bioRxiv - Microbiology 2022Quote: ... Petiole samples were lyophilized and ground to fine powder using a TissueLyzer II bead mill (Qiagen). DNA was extracted from ground tissue using a CTAB/phenol/chloroform extraction protocol as previously described (Wei et al ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
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bioRxiv - Pathology 2023Quote: ... at 35 Hz for 2 minutes with a sterile stainless steel 0.5 mm metal bead (Qiagen). Homogenized samples underwent depletion of host and bacterial ribosomal RNA using a modified previously published protocol (40) ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Neuroscience 2023Quote: ... was added to tissue and lysed using a sterile metal bead in a TissueLyser II (Qiagen). Tissue lysate in TRIzol was then mixed with UltraPureTM phenol:chloroform:isoamyl alcohol (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen) in a gravity flow column and then washed with 300mL of wash buffer A (25 mM HEPES pH 8.0 ...
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bioRxiv - Pathology 2023Quote: ... tissue lipids were extracted in bead-mill tubes (ceramic 1.4 mm, Mo-Bio, Qiagen, Germantown, MD) containing a solution of 225 µL MeOH ...
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bioRxiv - Microbiology 2023Quote: ... DNA was purified using an equal volume of SPRI beads and resuspended in EB buffer (Qiagen). DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Precellys tubes were prefilled with 600 mg of ceramic beads (1.4 mm, Qiagen, cat. no 1103955) to facilitate metabolite extraction ...
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bioRxiv - Plant Biology 2024Quote: ... coli under a T7 promoter and purified using Ni-NTA nickel affinity beads (Qiagen, Hilden Germany). Purified protein was dissolved in PBS solution and lyophilized ...
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bioRxiv - Bioengineering 2024Quote: ... Mechanical disruption was performed using the TissueLyser II and 8-mm stainless steel beads (both Qiagen) at an amplitude of 30 for 2 min at a time ...
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bioRxiv - Immunology 2024Quote: ... and minced before being homogenized using 5mm stainless steel beads using a Tissue Lyzer II (Qiagen). Protein content was quantified using a BCA test (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer’s instructions with an added cell lysis step at TissueLyser II bead beater (Qiagen), eluted in nuclease-free water ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were resuspended with zirconia/silica beads 0.1 mm diameter and disrupted mechanically (PowerLyzer 24 Qiagen) two cycles of 45s 5000 rpm with 5 minutes ice in between ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) and expression of the His-tagged Cas proteins was performed following the instruction of the protein purification kit (Qiagen, Valencia, CA, USA). Single colonies of transformed cells were cultivated overnight ...
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Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotencybioRxiv - Developmental Biology 2021Quote: Single embryo was subject to whole-genome amplification by using REPLI-g Mini Kits (QIAGEN, Cat. No. 150023). The target sites and 6 potential off-target sites (Table S4 ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were isolated from cell pellets using the EndoFree Plasmid Maxi Kit (0.45 g cells/column; Qiagen, 12362). Sequencing-based barcode quantification (see below Quantifying barcodes from plasmids and rabies virus genomes ...
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bioRxiv - Molecular Biology 2021Quote: ... the circular DNA (40 ng) was subjected to rolling circle amplification (RCA) using Repli-G midi kit (Qiagen). RCA is a technique used for multiple amplification of circular DNA to obtain sufficient amplicons for next-generation nucleotide sequencing (NGS ...
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bioRxiv - Molecular Biology 2021Quote: HEK293FT cells were transfected with a lentiviral vector with pVSV-G and pCMV8.2 using Effectene (QIAGEN, Hilden, Germany). Forty-eight hours after transfection ...
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bioRxiv - Genomics 2021Quote: ... the resulting cell pellet was subjected to whole genome amplification using the Repli-g® midi kit (Qiagen), after which the DNA was purified using QIAamp® DNA mini kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... The DNA for long-read MinION (ONT) sequencing was extracted using Genomic-tip 100/G (Qiagen, Hilden, Germany). The long-read sequencing libraries were prepared using 1D Ligation Barcoding Kit (SQK-LSK109 and EXP-NBD104 ONT ...
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bioRxiv - Microbiology 2022Quote: ... Whole transcriptome amplification of total RNA was carried out using RNA REPLI-g Cell WGA & WTA Kit (Qiagen) according to its protocol ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from 0.5 g sediment samples using RNeasy PowerSoil Total RNA Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions.