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Citations for Qiagen :
1401 - 1450 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). To purify JetAB complexes containing one full-length and one truncated JetA protomer ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen), eluted with 50 mM Tris-base ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysate [1μg/μL] was RNase A digested at 37°C for 15 min following addition of 1 μg RNase A (Qiagen) per 25 μg protein ...
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bioRxiv - Microbiology 2020Quote: Whole genome amplifications were performed on DNA extracts at dilution 10 times through a multiple displacement amplification (MDA) step of 6 to 7 hours using the REPLI-g Midi Kit (QIAGEN) and following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... was evaporated to half volume on a vacuum centrifuge (MAXI-DRY LYO, Heto) and 10 µL of the volume was treated with REPLI-g Mini (Qiagen) for 40 hours.
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication and the lysates were centrifuged at 52000 g for 45 min at 4°C.The supernatants were incubated with Ni-NTA agarose resin (Qiagen, Germany) for 2 hours before washing with 100 bed volume buffer A supplemented with 50mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 200,000 g for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen) to obtain total RNA including the small RNA fraction ...
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bioRxiv - Genetics 2021Quote: ... Single sperm or oocytes were transferred into single PCR tubes for amplification with the REPLI-g Single Cell kit (QIAGEN). RADseq libraries and whole genome libraries were prepared and sequenced on the HiSeq 3000 or NovaSeq S2 sequencing system (Illumina) ...
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bioRxiv - Genetics 2021Quote: ... and a pool of 20 males (F8ThaiF9Mex_M) were used to extract DNA using the QIAGEN Genomic tip 20/G kit (Qiagen, Germany). In another sample ...
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bioRxiv - Genomics 2021Quote: ... The supernatant was then used for high molecular weight (HMW) genomic DNA extraction according to the Genomic-tips protocol (100/G, Blood & Cell Culture DNA Midi Kit, QIAGEN).
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples of 50 milligrams of asexual KG females (approximately 120 individuals) were used for DNA extractions with Genomic-tip 100/G (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Single cells of Desulfovibrio or Methanococcus were sorted into wells of a 96-well plate containing 3 µl of PBS and Buffer D2 from Repli-G single cell kit (Qiagen) by using Influx flow cytometer (BD) ...
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bioRxiv - Genomics 2020Quote: WGA of single cells was performed by multiple displacement amplification using the REPLI-g Single Cell kit (Qiagen, Valencia, CA) as per the manufacturer’s instructions but at one quarter of the recommended volumes ...
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bioRxiv - Molecular Biology 2021Quote: ... MDA was done directly on cells (Supplementary figure 9) using the REPLI-g Single Cell Kit from Qiagen (Cat #150343) following kit-specific protocol ...
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bioRxiv - Genomics 2022Quote: An aliquot roughly corresponding to 0.2 g of lysate powder from the CRAC experiment was transferred to a separate tube and used to perform genomic extraction using the Genomic-tip 20/G kit (QIAGEN) following manufacturer’s specifications ...
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bioRxiv - Genomics 2020Quote: ... and template-switching oligo (5′-AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3′, where “r” indicates a ribonucleic acid base and “+” indicates a locked nucleic acid base; Qiagen). cDNA was amplified using KAPA HiFi HotStart ReadyMix kit (Roche #KK2502 ...
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bioRxiv - Genetics 2019Quote: ... gDNA was extracted from dried blood spots and whole-genome amplified using QIAmp DNA Mini kits and Replica-G Midi amplification kits (Qiagen), respectively ...
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bioRxiv - Genomics 2020Quote: High-molecular-weight DNA from white (20025) and yellow fat (70346) individuals was extracted from liver tissue using Genomic-tips (G/100) from Qiagen, and fragments smaller than 25Kb were progressively depleted using a Short Read Eliminator kit (Circulomics ...
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bioRxiv - Cancer Biology 2019Quote: ... The lysate was then centrifuged at 15 000 ×g and the supernatant was added to the 1 mL Ni-NTA resin (Qiagen). After washing with Buffer A supplied with 30 mM imidazole ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 to 10 g slices of liver tissue were homogenized in cell lysis solution and proteinase K (Qiagen, Germantown, USA). DNA was extracted from the liver homogenates and resuspended in buffer ...
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bioRxiv - Biochemistry 2019Quote: Genomic DNA was extracted from 2 g of frozen Annona muricata seed powder with the DNEasy Mericon Food Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The insoluble fraction was precipitated by ultracentrifugation (20,000 g) for 30 minutes at 4°C and the supernatant was loaded onto a Ni-NTA superflow affinity column (QIAGEN). The Ni-column was then wash three times and eluted with buffer containing 30 mM HEPES (pH 7.8) ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 0.8 µg of RNA was used for cDNA synthesis according to manufacturer’s instructions of the QuantiTect® Reverse Transcription Kit (Qiagen). 1 µl of cDNA was used for semi-quantitative reverse-transcriptase polymerase chain reaction ...
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bioRxiv - Cell Biology 2021Quote: Isolated Tert+ and Tert-cells (≤ 5 cells) were subjected to synthesize the complementary DNA (cDNA) using REPLI-g WTA Single Cell Kit (QIAGEN) and analyzed for gene expression by qRT-PCR ...
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bioRxiv - Genetics 2021Quote: ... These single cells or nuclei were whole-genome amplified by a REPLI-g Single Cell Kit (Qiagen, Venlo, the Netherlands) for 3 hours ...
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Gonads or body? Differences in gonadal and somatic photoperiodic growth response in two vole speciesbioRxiv - Neuroscience 2020Quote: ... frozen pieces of tissue (∼0.02 g) were homogenized in 0.5 ml TRIzol reagent in a TissueLyser II (Qiagen, Hilden, Germany) (2 x 2 minutes at 30 Hz ...
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bioRxiv - Genomics 2021Quote: ... a 1.0 μL aliquot of each sample was amplified in 10 μL reactions incubated at 30°C for 16 h as described in the REPLI-g Mini Kit (QIAGEN).
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 1 mL digestate slurry or 0.25 g soil using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) following a modified kit protocol where bead beating for 30s at 4.5 ms−1 in a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
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bioRxiv - Molecular Biology 2021Quote: Spider genomic DNA was extracted from dissected leg and cephalothorax tissue from adult female individuals using Genomic-tip 20/G (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: 293FT cells were transfected with pMSCV-dnCRTC or pMSCV-GFP constructs together with packaging plasmid pMD.MLV and pseudotyped envelope plasmid pMD2-VSV-G using Effectene transfection reagent (Qiagen #301425) as previously described ...
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bioRxiv - Biophysics 2022Quote: ... The lysates were centrifuged (15 min, 27000 ×g) and the supernatants were applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers (20 mM NaH2PO4 ...
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bioRxiv - Biophysics 2022Quote: ... The lysates were centrifuged (20 min, 27000 ×g) and the supernatants were applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers (20 mM NaH2PO4 ...
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bioRxiv - Biophysics 2022Quote: ... before rapidly cooling the cell suspension on ice.22 The lysate was centrifuged (15 min, 27000 g) and the supernatant was applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers (20 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were collected via centrifugation at 2500 ×g for 15 min and used for DNA purification using the DNeasy Blood & Tissue Kit (Qiagen), following the optional protocol for gram-negative bacteria provided by the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... the DNA was amplified directly from the bacterial pellet before the DNA extraction step using the REPLI-G Midi kit (Qiagen).
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bioRxiv - Genomics 2022Quote: ... The clean-up single-strand DNAs were processed to the MDA amplification by REPLI-g Single Cell Kit (Qiagen 150343). For Trueprime MDA-EM-seq ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from approximately 10 g of sediments for each depth using PowerMax Soil DNA Isolation Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Centrifugation was performed for 10 min at 10.000 x g and the top aqueous phase was further collected for RNA isolation using the RNeasy Mini Kit (Qiagen) according to the manufactures’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 x g for 10 min) and infected cells using a QIAamp Viral RNA MiniKit and RNeasy Plus mini kit (Qiagen), respectively ...
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bioRxiv - Microbiology 2020Quote: ... 30% or fewer HIV-1 DNA positive wellsSorted pools were then subjected to whole genome amplification by multiple displacement amplification (MDA) using the Advance Single Cell Repli-G kit (Qiagen), following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the beads were suspended in Buffer D2 and subjected to multiple displacement amplification (MDA) using a REPLI-g Single Cell Kit (QIAGEN).
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bioRxiv - Microbiology 2021Quote: High-molecular weight (HMW) genomic DNA was isolated from liquid cultures using the Qiagen Genomic-tip 20/G kit (Qiagen). DNA was then subjected to a hybrid approach of both short- and long-read DNA sequencing to achieve high-confidence complete de novo genome assemblies ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was extracted from 0.25 g of an ethanol-free sample in duplicates using the DNeasy PowerSoil kit (QIAGEN, RRID:SCR_008539). The extraction protocol followed the manufacturer’s guidelines with several modifications (as described below) ...
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bioRxiv - Microbiology 2022Quote: ... for Illumina sequencing most samples were subjected to whole-genome amplification (WGA) with the REPLI-g Single Cell Kit (Qiagen), either directly from few ciliate host cells (four samples ...
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bioRxiv - Biophysics 2022Quote: ... Insoluble material was pelleted for 1 hr at 100,000 x g and the supernatant was added to Ni-NTA resin (QIAGEN, 30410) in batch and was eluted with an imidazole gradient containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... reaction was conducted after pressure ejection of the cytoplasm into a microcentrifuge tube containing the REPLI-g WTA single cell kit (Qiagen). Each cell was incubated in a total volume of 5.5 μl at 24°C for 5 min and then cooled to 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Individual single Giardia cysts or pools of 10 cysts isolated by FACS were subjected to MDA with the REPLI-g single cell kit (Qiagen). MDA was performed according to the manual ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was centrifuged (20 min, 27000 g) and the supernatant was applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers containing increasing concentrations of imidazole (20-50 mM ...
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bioRxiv - Zoology 2021Quote: Frozen single cells in PCR tubes were thawed and subjected to lysis and multiple displacement amplification (MDA) using the REPLI-g UltraFast Mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pelleted at 350 x g for 5 min at 4 °C and lysed in 350 μl RLT buffer of the RNeasy Mini Kit (Qiagen). RNA was isolated according to the manufacturer’s instructions ...