Labshake search
Citations for Qiagen :
1101 - 1150 of 2369 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... All samples from bead-based assays were performed using a LiquiChip-200 instrument (Qiagen, Hilden, Germany) using Bio-Plex Manager v6.1 software for quantification.
-
bioRxiv - Immunology 2022Quote: ... primers followed by reverse phase 0.65 x SPRI beads purification and a QIAquick Spin Column (QIAGEN) purification ...
-
bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
-
bioRxiv - Microbiology 2022Quote: ... Petiole samples were lyophilized and ground to fine powder using a TissueLyzer II bead mill (Qiagen). DNA was extracted from ground tissue using a CTAB/phenol/chloroform extraction protocol as previously described (Wei et al ...
-
bioRxiv - Pathology 2023Quote: ... at 35 Hz for 2 minutes with a sterile stainless steel 0.5 mm metal bead (Qiagen). Homogenized samples underwent depletion of host and bacterial ribosomal RNA using a modified previously published protocol (40) ...
-
bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen) in a gravity flow column and then washed with 300mL of wash buffer A (25 mM HEPES pH 8.0 ...
-
bioRxiv - Neuroscience 2023Quote: ... was added to tissue and lysed using a sterile metal bead in a TissueLyser II (Qiagen). Tissue lysate in TRIzol was then mixed with UltraPureTM phenol:chloroform:isoamyl alcohol (ThermoFisher ...
-
bioRxiv - Bioengineering 2023Quote: ... Mechanical disruption was performed using the TissueLyser II and 8-mm stainless steel beads (both Qiagen) at an amplitude of 30 for 2 min at a time ...
-
bioRxiv - Pathology 2023Quote: ... tissue lipids were extracted in bead-mill tubes (ceramic 1.4 mm, Mo-Bio, Qiagen, Germantown, MD) containing a solution of 225 µL MeOH ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was purified using an equal volume of SPRI beads and resuspended in EB buffer (Qiagen). DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina ...
-
bioRxiv - Neuroscience 2023Quote: ... Precellys tubes were prefilled with 600 mg of ceramic beads (1.4 mm, Qiagen, cat. no 1103955) to facilitate metabolite extraction ...
-
bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
-
bioRxiv - Plant Biology 2024Quote: ... coli under a T7 promoter and purified using Ni-NTA nickel affinity beads (Qiagen, Hilden Germany). Purified protein was dissolved in PBS solution and lyophilized ...
-
bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) and expression of the His-tagged Cas proteins was performed following the instruction of the protein purification kit (Qiagen, Valencia, CA, USA). Single colonies of transformed cells were cultivated overnight ...
-
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotencybioRxiv - Developmental Biology 2021Quote: Single embryo was subject to whole-genome amplification by using REPLI-g Mini Kits (QIAGEN, Cat. No. 150023). The target sites and 6 potential off-target sites (Table S4 ...
-
bioRxiv - Neuroscience 2021Quote: ... Plasmids were isolated from cell pellets using the EndoFree Plasmid Maxi Kit (0.45 g cells/column; Qiagen, 12362). Sequencing-based barcode quantification (see below Quantifying barcodes from plasmids and rabies virus genomes ...
-
bioRxiv - Molecular Biology 2021Quote: ... the circular DNA (40 ng) was subjected to rolling circle amplification (RCA) using Repli-G midi kit (Qiagen). RCA is a technique used for multiple amplification of circular DNA to obtain sufficient amplicons for next-generation nucleotide sequencing (NGS ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293FT cells were transfected with a lentiviral vector with pVSV-G and pCMV8.2 using Effectene (QIAGEN, Hilden, Germany). Forty-eight hours after transfection ...
-
bioRxiv - Genomics 2021Quote: ... the resulting cell pellet was subjected to whole genome amplification using the Repli-g® midi kit (Qiagen), after which the DNA was purified using QIAamp® DNA mini kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: ... The DNA for long-read MinION (ONT) sequencing was extracted using Genomic-tip 100/G (Qiagen, Hilden, Germany). The long-read sequencing libraries were prepared using 1D Ligation Barcoding Kit (SQK-LSK109 and EXP-NBD104 ONT ...
-
bioRxiv - Microbiology 2022Quote: ... Whole transcriptome amplification of total RNA was carried out using RNA REPLI-g Cell WGA & WTA Kit (Qiagen) according to its protocol ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was extracted from 0.5 g sediment samples using RNeasy PowerSoil Total RNA Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... 0.25 g soil was used for DNA extraction using a DNeasy PowerLyzer PowerSoil kit (Qiagen Inc., Germantown, MD) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Whole genome amplification was performed by multiple displacement amplification with the REPLI-g single cell kit (Qiagen, #150345), according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... we extracted total genomic DNA using the Qiagen Genomic Tip 20/G gravity flow columns (Qiagen, Germantown, MD) using the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: Total DNA was extracted from 0.25 g of each soil sample by using a DNeasy PowerSoil Kit (Qiagen) and QIAcube (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... DNA was extracted from 10 g of sediment using the PowerMax Soil DNA Isolation Kit (12988-10, QIAGEN) according to the manufacturer’s protocol with minor modifications for the step of homogenization and cell lysis i.e. ...
-
bioRxiv - Microbiology 2019Quote: ... Nucleic acid extractions were performed on approximately 0.25 g of each sample using the Qiagen DNeasy Powersoil DNA Isolation kit following the manufacturer’s instructions (Qiagen). The lysing step was performed using the Disruptor Genie cell disruptor (Scientific Industries) ...
-
bioRxiv - Genomics 2021Quote: ... exigua pupa using the Qiagen Genomic-tip 100/G kit according to the manufacturer’s instructions (Qiagen, Venlo, Netherlands). Quality of the extracted HMW DNA was analyzed on an Agilent 4200 TapeStation System using Genomic DNA ScreenTape (Agilent ...
-
bioRxiv - Genomics 2020Quote: ... Whole genome amplification was performed by multiple displacement amplification (MDA) using the REPLI-g Single Cell Kit (QIAGEN) according to protocols provided by the manufacturer ...
-
bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from an overnight culture using the Genomic-tip 20/G kit (Qiagen GmbH, Germany). Sequencing was performed with the PacBio RS II system based on single molecule ...
-
bioRxiv - Genomics 2021Quote: ... DNA was extracted from an overnight culture using the QIAGEN genomic tip 100/G kit (Qiagen®, #10243) according to manufacturing protocol ...
-
bioRxiv - Microbiology 2021Quote: Genomic DNA was amplified from all samples using a REPLI-g Single Cell Kit (Qiagen, Germantown, MD, USA) using UV-treated sterile plasticware and reverse transcription-PCR grade water (Ambion ...
-
bioRxiv - Microbiology 2022Quote: Total DNA was extracted from 0.25 g of soil with the DNeasy PowerSoil Pro kit (Qiagen, Hilden, Germany), following the manufacturer’s instructions ...
-
Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... 440 μL of MDA reaction mixture containing 290 μL REPLI-g sc Reaction Buffer (Qiagen, catalog no. 150345), 20 μL REPLI-g sc DNA Polymerase (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: Genomic DNA from the pooled library samples was extracted using a Qiagen Genomic-tip 100/G kit (Qiagen) and used to prepare TraDIS sequencing libraries as previously described (25) ...
-
bioRxiv - Microbiology 2022Quote: Total community DNA was extracted from 1 g of crushed rocks using DNeasy PowerSoil Pro Kit (Qiagen, German), quality checked by electrophoresis using a 1.5% agarose gel and Nanodrop spectrophotometer (Thermofisher ...
-
bioRxiv - Microbiology 2022Quote: ... bacterial cells reaching an OD620nm = 0.6 were pelleted (5000 x g, 15 min) and immediately fixed in 1.5 ml RNA-protect (Qiagen) for 10 min before being processed using a PureLink RNA mini kit (Ambion ...
-
bioRxiv - Microbiology 2023Quote: ... 5μL of the resulting DNA underwent one or more displacement amplifications using the Repli-G MDA kit (Qiagen), to enrich microbial DNA ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 5-10 g of soil per sample with the DNeasy PowerMax Soil Kit (Qiagen) and used for short read and long read sequencing ...
-
bioRxiv - Genetics 2023Quote: ... Whole genome amplification was done using Multiple Displacement Amplification (MDA)18,19 (Qiagen REPLI-g kit catalog no. 150345) and the BioSkryb Primary Template Amplification (PTA ...
-
bioRxiv - Developmental Biology 2023Quote: ... with the addition of a 2-minute spin at 15,000 x g in the QIAshredder (Qiagen, Germantown, MD) prior to the genomic DNA elimination step ...
-
bioRxiv - Genomics 2023Quote: Total RNA was extracted from approximately 0.1 g whole rosette tissues using the RNeasy Plant Mini Kit (Qiagen). RNA samples were quantified using a NanoDrop spectrophotometer (NanoDrop Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting DNA was amplified by multiple displacement amplification (MDA) using a REPLI-g Single Cell Kit (QIAGEN) following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... regius cells harboring cyanobacterial symbionts was subjected to independent genome amplification using REPLI-g Single Cell Kit (QIAGEN), and the amplified DNA samples were processed with S1 nuclease (TaKaRa ...
-
bioRxiv - Genomics 2024Quote: ... To extract high molecular weight DNA a Genomic-tip 20/G kit (Qiagen Benelux BV, Venlo, the Netherlands) was used according to the standard protocol ...
-
bioRxiv - Microbiology 2023Quote: ... After centrifugation (30 min, 100,000 x g, the cleared lysate was purified via Ni-NTA agarose columns (Qiagen) and eluted with a discontinuous imidazole gradient using an ÄktaGo system ...
-
bioRxiv - Microbiology 2024Quote: ... 2.5 µl of the preparation was used for whole-genome amplification with REPLI-g Single Cell Kit (Qiagen). Subsequently ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Lysates were incubated for 1 hr at 37°C after adding 10 µl of RNAse A (20 mg/ml) and proteins were precipitated with 200 µl of Protein Precipitation Solution (Qiagen Gentra Puregen Cell kit). After centrifugation ...