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Citations for Qiagen :
1101 - 1150 of 1556 citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen) following the instructions of the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Plant Biology 2020Quote: ... and the GST fusion proteins were purified by Glutathione HiCap Matrix columns (Qiagen) and eluted using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was analyzed by immunoblotting using monoclonal anti-RGSH4 antibodies (Qiagen) to detect ABCG5 and polyclonal anti-hABCG8 antibodies (Novus Biologicals ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The recombinant proteins were purified under denaturing conditions on Ni-NTA agarose (Qiagen). The purified proteins were used for rat immunization in an in-house animal facility at the Charles University.
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with Ni-NTA agarose resin (Qiagen, 30210) for four hours ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen). Proteins were eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... All proteins were purified under native conditions by using Ni-NTA sepharose (QIAGEN) according to the manual.
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bioRxiv - Immunology 2021Quote: ... The proteins from filtered culture supernatants were purified by Ni-NTA column (QIAGEN) and size exclusion chromatography (HiLoad Superdex 75 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified by metal affinity chromatography on a Ni2+-NTA column (Qiagen). Cell free extract was loaded onto column followed by two wash steps with wash buffer (Tris 50 mM ...
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bioRxiv - Biochemistry 2021Quote: ... Briefly proteins were purified by glutathione-Sepharose (Cytiva) or Ni-NTA agarose (Qiagen) affinity chromatography ...
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bioRxiv - Microbiology 2021Quote: ... and the protein was purified using a Ni–NTA column (Qiagen, Hilden, Germany) as reported previously 49 ...
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bioRxiv - Microbiology 2020Quote: ... The RBD protein was purified by affinity chromatography using Ni-NTA resin (QIAGEN), followed by size exclusion chromatography on a HiLoad Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble recombinant protein was purified using theNickel-Nitrilotriacetic acid (Ni-NTA+; Qiagen) resin ...
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bioRxiv - Cell Biology 2021Quote: ... The protein interaction network analysis was performed using QIAGEN’S Ingenuity Pathways Analysis (QIAGEN’S Ingenuity pathway analysis ...
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bioRxiv - Bioengineering 2022Quote: ... Purification of all proteins was performed using a Ni-NTA agarose column (Qiagen). After washing with buffer A containing 30 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Proteins were extracted from frozen mouse left ventricles using the TissueLyser LT (Qiagen) with 5mm stainless steel beads (69989 ...
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bioRxiv - Molecular Biology 2023Quote: The eluted protein was mixed with 1 ml Strep-Tactin Superflow agarose (Qiagen) and incubated at 4 °C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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bioRxiv - Neuroscience 2023Quote: Protein pathway analysis was conducted on DEP using Ingenuity Pathway Analysis (IPA, Qiagen) and Gene Ontology (GO ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was isolated from the lysate using Ni-NTA Agarose resin (Qiagen) and was purified to homogeneity and >95% purity by size exclusion chromatography using a HiLoad Superdex 75 pg 16/600 column (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified through metal-affinity chromatography using Ni2+–NTA agarose beads (Qiagen). Purified protein aliquots were stored at -30°C in 50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2023Quote: ... all proteins were purified using Ni-NTA (nickel-nitrilotriacetic acid) resin from QIAGEN. After the incubation of Expi293 media supernatant with the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under non-denaturing conditions using Ni-NTA resin (Qiagen) at 4 °C according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Physiology 2023Quote: ... Tissues were then lysed in protein isolation buffer using a tissue-lyser (Qiagen). Protein concentration was measured by Bradford assay ...
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bioRxiv - Biophysics 2023Quote: ... His6-MBP-HNRNPH2 proteins were first purified using Ni-NTA Agarose (#30230, Qiagen) and eluted with buffer containing 250 mM imidazole pH 7.8 (#79227 ...
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bioRxiv - Cancer Biology 2022Quote: ... EV and protein fractions using miRNeasy serum/plasma kit (Qiagen 1071073 Lot#160020206) after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035) ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Nickel sepharose according to the manufacturer’s instructions (QIAGEN). The purified recombinant protein was dialyzed overnight against dialysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by immobilized metal affinity chromatography using Ni–NTA agarose (Qiagen) pre-equilibrated with lysis buffer in individual Econo-Pac gravity-flow columns (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: SAINT Express protein hits were analyzed using Ingenuity Pathway Analysis (IPA) software (Qiagen). Significant hits were analyzed and enrichment z-score was calculated for different pathways ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant TDP-43 proteins were purified over Ni-NTA agarose beads (Qiagen) and eluted using 50 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... All nucleotide and protein sequences were analyzed using CLC Main Workbench 8.1.2 (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... The His6-tagged proteins were purified using Ni-NTA beads (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The His6-tagged proteins were purified using Ni-NTA beads (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The His6-tagged proteins were purified using Ni-NTA beads (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was again loaded onto a NiNTA 10/50 column (Qiagen, Germany) and subsequently subjected to size exclusion chromatography using Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates from snap-frozen tissue were prepared using the TissueLyser II (Qiagen) and RIPA lysis buffer (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA (200 ng) was used for quantitative PCR with the Human Cellular Senescence RT2 Profiler™ PCR Array (Qiagen) containing 84 key genes involved in the initiation and progression of the biological process causing cells to lose the ability to divide ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from the cell pellets of human primary male and female PT cells using the RNAeasy Mini Kit (Qiagen). After quantifying RNA concentration in a Nanodrop instrument (Thermo) ...
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bioRxiv - Cancer Biology 2019Quote: ... was isolated from human PanNET specimens or cells grown on 6-cm or 10-cm plates using RNeasy mini kit (Qiagen) containing gDNA eliminator spin columns ...
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bioRxiv - Molecular Biology 2019Quote: Preliminary screening for the presence of serum exosomal miRNAs was determined using a miScript human miFinder PCR array (MIHS-001Z, Qiagen). RNA was converted to cDNA using miScript RT kit (218060) ...
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bioRxiv - Plant Biology 2019Quote: ... three replicates of 25 to 30 pooled egg cells were used for total RNA extraction according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (Qiagen). In brief ...
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bioRxiv - Genetics 2020Quote: ... Analyses of HTT CAG repeat size in both HttQ111 mice and in patient fibroblasts was performed by PCR using human-specific HTT primers and Taq PCR Core Kit with Q solution (Qiagen), as previously described (17 ...
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bioRxiv - Bioengineering 2020Quote: ... The prepared cDNA was preamplified using the RT2 PreAMP Primer Mix for Human and Mouse PCR Array (Qiagen, PBH-181Z). cDNA was analyzed by RT-qPCR using a Qiagen RT2 profiler custom panel (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Total RNA of 1-2 x 106 human macrophages (2-4 biological replicates/ macrophage donors per condition, see Table S17) was isolated using RNeasy extraction kit (Qiagen) including DNAse treatment according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Synthetized cDNA was subjected to a PCR array specific for the human antiviral response (RT² Profiler PCR array – PAHS-122Z, SA Biosciences, Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... was used for human ISG15 and ISG56 and mouse genes including Gapdh as the housekeeping gene on the Rotor-Gene Q 5plex (Qiagen). RT-qPCR primers and probes are listed in the supplementary table 2.