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Citations for Qiagen :
901 - 950 of 1556 citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Initial crystallization conditions were obtained with the Protein Complex Suite (Qiagen) screened by sitting drop-vapor diffusion at 18 °C using a Mosquito robot and were optimized in hanging drops by mixing 1 μL of the sample with 1 μL of the precipitant ...
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bioRxiv - Genomics 2021Quote: ... The proteins were bound to a Ni-NTA sepharose beads (Qiagen), extensively washed with binding buffer and eluted in buffer containing 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... and proteins were purified with Ni-NTA resin (Qiagen, Cat# 151010181) and followed by Superose 6 10/300 gel filtration column chromatography (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The solubilized protein was then purified using Ni-NTA agarose (Qiagen). The purity was confirmed (>98% ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen), the eluted fractions containing S protein were pooled ...
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bioRxiv - Microbiology 2021Quote: ... cruzi proteins attached to addressable Liquichip Ni-NTA beads (Qiagen, CA) and T ...
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bioRxiv - Molecular Biology 2021Quote: ... The fusion protein was affinity purified using Ni-TNA agarose (Qiagen). Details are provided in the Supplementary Information methods section.
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bioRxiv - Microbiology 2022Quote: ... Protein was purified under native conditions according to the QIAexpressionist (Qiagen) protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). To purify JetAB complexes containing one full-length and one truncated JetA protomer ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen), eluted with 50 mM Tris-base ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified from sonicated lysates using Ni-NTA resin (Qiagen) and gravity flow ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Novex, Qiagen) using MOPS running buffer (Novex ...
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bioRxiv - Developmental Biology 2023Quote: ... After the protein digestion with Proteinase K (QIAGEN, Cat. No. #158920) and RNA digestion with RNase A (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Cell Biology 2023Quote: ... and AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen). Isolated DNA and RNA samples were quantified by Nanodrop (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen) and a gravity column (Biorad) ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: SNAP-GAF protein was purified on Ni-NTA agarose (Qiagen 30210), followed by Amylose resin (NEB cat ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were purified using a two-column protocol: Ni-NTA (Qiagen) affinity chromatography followed by size exclusion chromatography (HiLoad Superdex 75 pg or 250 pg ...
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bioRxiv - Genomics 2020Quote: ... High molecular weight DNA was isolated from each coral tissue sample using the Qiagen DNeasy kit (Qiagen, Valencia, CA) without prior enrichment for S ...
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bioRxiv - Neuroscience 2020Quote: ... Endotoxin free plasmid preparations of high yield and purity for electroporation was prepared by maxi-prep (Qiagen, Hilden, Germany).
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bioRxiv - Genomics 2022Quote: ... The pellets were used to extract high molecular weight DNA using Gentra Puregene yeast/bacteria DNA isolation kit (Qiagen), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: The pEGFP-attB vector with the priEE fragment was prepared at a high concentration using Plasmid Midi Kit (Qiagen) and transported to WellGenetics (Taiwan ...
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bioRxiv - Evolutionary Biology 2021Quote: High molecular weight DNA was isolated from fin tissue preserved in ethanol using QIAamp DNA Mini Kit (Qiagen, Germany) or obtained by purification of salt method extracted DNA (Aljanabi & Martinez ...
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bioRxiv - Genomics 2021Quote: ... High-quality genomic DNA was extracted from liquid cultures using the DNeasy Plant Mini Kit from Qiagen (Venlo, Netherlands). The isolates were sequenced on an Illumina HiSeq in paired-end mode and raw reads were deposited at the NCBI SRA under the BioProject PRJNA596434.
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bioRxiv - Genomics 2021Quote: High molecular weight DNA was extracted from 100 µl of blood using the DNeasy Blood and Tissue kit (Qiagen). For long read (Oxford Nanopore ...
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bioRxiv - Immunology 2020Quote: ... Gene ontology analyses for high affinity/SHM class-specific GC B cells were performed with Ingenuity Pathway Analysis (Qiagen) software using avg_logFC values of all genes significantly enriched in at least one class ...
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bioRxiv - Microbiology 2022Quote: High molecular weight (>20 kb fragment) genomic DNA was prepared using the Qiagen MagAttract HMW DNA Kit (Qiagen 67563), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... High molecular weight DNA was extracted from liver tissue using a Genomic-tip 20/G DNA kit (QIAGEN, 10223).
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bioRxiv - Plant Biology 2022Quote: ... were collected in March 2018 and used for high-molecular-weight DNA extraction with DNeasy Plant Mini Kit (Qiagen). A paired-end (PE ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearized DNA was extracted using phenol/chloroform and centrifugation in MaXtract High-density phase separation tubes (Qiagen #129046), followed by overnight precipitation with ammonium acetate and 100% ethanol at -20ºC ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Molecular Biology 2022Quote: ... following manufacturer’s instructions except after adding chloroform the sample was transferred to pre-spined MaXtract High Density (Qiagen #129065) and centrifuged at 12,000g for 15 mins at 4°C to phase sperate the aqueous phase from the organic phase ...
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bioRxiv - Genomics 2023Quote: ... RNA was initially extracted following the Qiazol manufacturer’s protocol using the optional 2mL MaXtract High-density gel tubes (Qiagen) to obtain the crude aqueous fraction ...
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bioRxiv - Genomics 2023Quote: ... high-quality genomic DNA of each sample was extracted by using a DNeasy Plant Mini Kit (Qiagen, United States). The DNA sequencing libraries were constructed following the procedures provided by the Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2023Quote: High-molecular-weight (HMW) DNA was extracted from 30M frozen pelleted cells using the Gentra Puregene extraction kit (Qiagen). Purified gDNA was assessed using fluorometric (Qubit ...
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bioRxiv - Genomics 2022Quote: ... We extracted High-Molecular Weight Genomic DNA (HMW DNA) from the dissected bacteriomes with MagAttract HMW DNA Kit (QIAGEN). Metagenomic libraries of three species were prepared with NEBNext Ultra II DNA Library Prep Kit (BioLabs ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: High molecular-weight sperm DNA collected from F0 males was obtained using MagAttract HMW DNA Kit (QIAGEN, Cat # 67563), following a modified version of the manufacturer’s instructions which consisted of replacing the digestion step with Proteinase K with 40 µL of the reducing agent TCEP (Tris(2-carboxyethyl)phosphine hydrochloride ...
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bioRxiv - Microbiology 2023Quote: ... Genomic high-molecular-weight DNA was extracted from cell pellets using MagAttract R HMW DNA Kit (Qiagen, Hilden, Germany) to a final elution volume of 100 μL ...
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bioRxiv - Genomics 2024Quote: The resuspended protoplasts were then used as input for the MagAttract high molecular weight DNA extraction kit (67563, Qiagen), which was used with the manufacturer’s protocol for blood cells with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... Conversion of total mRNA to cDNA was performed using a High-Capacity cDNA Reverse Transcription Kit (Qiagen, Hilden, Germany).
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequence reads were mapped against the human reference genome (NCBI build 37/hg19) using CLC Genomics Workbench (version 5.0.1) software (QIAGEN). Mutation analysis was performed under recessive ...
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bioRxiv - Microbiology 2019Quote: Microbial genomic DNA was extracted from the human stool samples using the DNeasy PowerSoil DNA Isolation Kit (Qiagen). The V4 region of 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform(67) ...
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bioRxiv - Molecular Biology 2019Quote: ... in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT) (RT2 qPCR Primer Assays, Qiagen) mRNA when performing siRNA analyses.
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bioRxiv - Genetics 2019Quote: ... The list of human orthologs was analyzed for pathway enrichment by Ingenuity Pathway Analysis software (Qiagen, Hilden, Germany).
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bioRxiv - Cell Biology 2019Quote: ... transfected with 100 ng of human TfR (and 100ng of rescue construct if applicable) using the Effectene (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... human HDL (40nM) or PBS for 48 hours prior to RNA isolation using the RNeasy Mini kit (Qiagen). RNA samples were converted to cDNA libraries by the Northwestern University Genomics Core facility and were then run on the Illumina HT-12 microarray ...
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bioRxiv - Microbiology 2021Quote: ... Cell-free cfDNA (both human and mouse) was isolated with QIAamp DSP Circulation NA Kit (QIAGEN, Hilden, Germany) following the manufacturer’s protocol ...