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Citations for Qiagen :
951 - 1000 of 1556 citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 22 human vaginal samples using the QIAamp UCP Pathogen Mini Kit (QIAGEN, Venlo, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: HeLa cells (2×106) were transfected with 100 pmol of validated siRNAs against all human ZDHHCs [42] (Qiagen) using interferrin transfection reagent (Polyplus) ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA from murine or human monocytes was isolated using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, 80224). DNA (1 µg ...
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bioRxiv - Neuroscience 2021Quote: The total RNA from mouse cortex and human PBMC was extracted using the RNeasy® Mini Kit (Qiagen) and real-time PCR was done as described in the Supplementary material.
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bioRxiv - Neuroscience 2020Quote: ... RNA was isolated from human autopsy brain tissue using the RNeasy Plus Universal Mini Kit (Qiagen Cat# 73404).
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from cells or human or mouse brain tissues using RNeasy Mini kit (Qiagen, 74106). Reverse transcription was carried out using iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... knockdown of KCNB1 expression in human cells was carried out using a mixture of 4 siRNA duplexes (Qiagen; Cat# ...
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bioRxiv - Immunology 2020Quote: ... RT2 profiler PCR array of human NF-κB signaling targets was performed as instructed by the manufacturer (Qiagen).
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bioRxiv - Genomics 2021Quote: ... The manufacturer’s protocol was followed for manual purification of total RNA from human whole blood (Qiagen, cat #762164). RNA was eluted with RNase free water ...
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bioRxiv - Neuroscience 2021Quote: Microdissected mouse and human brain tissue was flash frozen and homogenized in Trizol using a TissueLyser II (Qiagen). Phenol chloroform extraction was then used together with the RNeasy mini kit (QIAGEN ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR was performed using QIAGEN’s RT2 Profiler™ PCR Array Human Cell Death PathwayFinder (QIAGEN, Hilden, Germany). RT-PCR data was analyzed and visualized for pathway analysis by GeneSpring GX 14.9 (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from the human biopsy samples and mice colon tissue was isolated using RNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Human myeloma cell lines were authenticated by subjecting genomic DNA isolated with the QIAamp DNA Mini Kit (Qiagen), and short tandem repeat (STR ...
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bioRxiv - Developmental Biology 2024Quote: ... Reads from *.fastq files were mapped to the human reference genome (GRCh37) using CLC Genomics Workbench 12.0 (Qiagen). Transcript abundance was expressed as reads per kilobase of transcript per million mapped reads (RPKM) ...
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bioRxiv - Neuroscience 2023Quote: ... Bulk gDNA and RNA from human retinal organoids were isolated using the AllPrep DNA/RNA Micro Kit (Qiagen) or DNeasy Blood & Tissue Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: SiRNAs targeting human MSI2 (Supp Table S3) and nonspecific control pool siRNAs were purchased from Qiagen (Frederick, MD). Cultured cells at 50% confluence were transfected with siRNA at final concentrations of 50 nmol/L using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was collected from treated human or murine primary spinal cord astrocytes using an RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from human glioblastoma cells using the RNeasy kit according to the manufacturer’s instructions (Qiagen). RNA quality was assessed on a Bioanalyzer (Agilent ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from the frozen human stomach tissues using the RNeasy plus Mini Kit (Qiagen, US) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reads were aligned to the GRCm38 build of the human genome by using CLC Genomics Workbench v10 (Qiagen).
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bioRxiv - Genomics 2024Quote: Genomic DNA from human CD34+ cells from three normal adult donors was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Primary human Fibroblasts were washed with PBS before total RNA extraction using the RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: RNA from human cell cultures was extracted from 6 well plates using RNeasy Plus micro kit (Qiagen, 74034). The plates were washed once with PBS ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from human CSF samples using the PAXgene® Blood miRNA kit (Pre-Analytix, Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: The ipsilateral hemispheres were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Microbiology 2021Quote: The cells were lysed by high speed vortexing for 2 minutes using lysing matrix E tubes (MP Biomedical) in buffer RLT (Qiagen) amended with 1% 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were lysed in Qiazol Lysis Reagent and total RNA was extracted using the MaXtract High Density kit with further purification using the RNeasy Mini Kit (all Qiagen). 70 ng of total RNA per sample was then hybridized with reporter and capture probes for nCounter Gene Expression code sets (Mouse Neuroinflammation codeset ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitates (IPs) were washed in high salt buffer and RNA from inputs and IPs extracted using the RNeasy micro kit (Qiagen).
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were subsequently cloned into pGEM®-T Easy Vector plasmids in a JM109 High- Efficiency Competent Cells (Qiagen) strain of Escherichia coli ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we extracted high-molecular weight (HMW) DNA from 200 uL fresh blood using the MagAttract HMW DNA mini kit (Qiagen), following the Whole Blood protocol ...
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bioRxiv - Genomics 2022Quote: ... The biotinylated single-stranded PCR products were then purified with Streptavidin Sepharose High Performance beads (Cytiva) and hybridized to sequencing primers (same as forward) on the PyroMark vacuum prep workstation (QIAGEN). Finally ...
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bioRxiv - Microbiology 2020Quote: ... All DNA purifications were performed using a Roche High Pure PCR product purification kit or a QIAquick Gel Extraction Kit (Qiagen). Standard procedures for DNA digestion and ligation were used in conditions recommended by the enzyme manufacturer (Promega or Fermentas) ...
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bioRxiv - Cancer Biology 2019Quote: Cells were harvested and high molecular weight DNA was extracted using the MagAttract HMW DNA Kit (Qiagen N.V., Venlo, Netherlands). Size selection was performed to remove fragments <10 kilobases (kb ...
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bioRxiv - Microbiology 2021Quote: High-molecular weight (HMW) genomic DNA was isolated from liquid cultures using the Qiagen Genomic-tip 20/G kit (Qiagen). DNA was then subjected to a hybrid approach of both short- and long-read DNA sequencing to achieve high-confidence complete de novo genome assemblies ...
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bioRxiv - Microbiology 2022Quote: ... was extracted from a high titre phage stock (~1010 PFU/mL) and overnight bacterial cultures using the DNAeasy Blood and Tissue Kit (QIAGEN) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Dovetail staff extracted high molecular weight (HMW) DNA from tissues using the Blood and Cell Culture Midi Kit (Qiagen, GmbH) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... High-quality total RNA was extracted from the right hemisphere of the brain using RNeasy Plus Universal Midi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... pentatrifurcatum 38,39 cells in culture dishes (50 Petri dish cultures) using MagAttract high-molecular-weight (HMW) DNA kit (Qiagen, MD), following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: DNA from fecal samples and bacterial cultures was extracted using the MoBio High Throughput (HTP) PowerSoil Isolation Kit (MoBio Laboratories; now QIAGEN) with minor modifications ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was collected from multiple stages of zebrafish development (30%-epiboly, 9 somite, Prim-5, High-pec and Larvae) using the miRNeasy kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The cell lysate was clarified by high-speed centrifugation and the clarified lysate incubated with Ni-NTA agarose resin (Qiagen) for 2 h at 4 °C using a rotator ...
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bioRxiv - Genomics 2021Quote: ... DNA from ChIP and input samples were isolated with Phenol-chloroform using MaXtract High Density tubes (Qiagen Catalog No. 129046) to maximize sample retrieval ...
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bioRxiv - Genomics 2021Quote: ... For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Genomics 2022Quote: Genomic DNA from aposymbiotic animals stored in RNAlater was extracted using MagAttract High Molecular Weight DNA extraction kit from Qiagen following manufacture’s protocol with some modifications ...
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bioRxiv - Genetics 2022Quote: High molecular weight (HMW) genomic DNA from 538 F2 spleen samples was isolated via the MagAttract HMW DNA Kit (Qiagen) at the University of Michigan and transferred to the University of California San Diego for further processing ...
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bioRxiv - Microbiology 2023Quote: ... We could not get a PCR product for the R escaper and therefore extracted the total DNA from the escaper and the ancestor λ-vir phage lysate after enrichment for high phage titer (> 107 pfu/ mL) DNA with the DNeasy blood and tissue kit (QIAGEN), starting from the Proteinase-K treatment step to lyse the phages 92 ...
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bioRxiv - Cancer Biology 2023Quote: ... high-quality genomic DNA was isolated from whole epithelia using the DNeasy Blood and Tissue Kit per manufacturer protocol (Qiagen). PCR products were generated using REDTaq® polymerase and LSL excision primers ...
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bioRxiv - Genomics 2023Quote: ... Whole-genomic DNA with high purity and quality was extracted from the tissue samples using DNeasy Blood & Tissue Kits (QIAGEN). We generated >25x coverage whole-genome short-read paired-end data (using Illumina Novaseq ...
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bioRxiv - Plant Biology 2023Quote: Detection of SNPs as genetic markers were performed by high-resolution melt analysis (HRM) by using the Rotor Gene Technology (Qiagen). PCR was carried out in 20 µl volume containing 20 ng template DNA ...