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Citations for Qiagen :
1051 - 1100 of 1556 citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... before RNA isolation using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: SacSm-containing protein sample was loaded on a Ni-NTA Agarose (Qiagen) column equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified from the supernatant via nickel affinity chromatography (Qiagen). The protein was further purified by a Hiload 16/60 Superdex 200 column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by affinity chromatography using Ni-NTA agarose (Qiagen) resin followed by his tag cleavage by TEV protease and gel filtration using Superdex 200 Increase 10/300 GL column (G.E ...
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bioRxiv - Biochemistry 2024Quote: ... Protein-digested DNA was purified using a PCR Clean-up kit (Qiagen). Purified DNA was treated with 100 nM TDP2 in 30 µL of 1X rCutsmart buffer (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... The fusion protein was purified using Ni-NTA Resin Spin Columns (QIAGEN). Eurogentec (Belgium ...
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bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... and human BON1 cell line [8] were isolated with RNeasy Plus Universal Kits (Qiagen Cat no. 73404, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA expression of 84 apoptotic genes was analyzed using the RT2 Profilμer PCR Human Apoptosis Array (Qiagen, PAHS-012Z). Arrays were prepared according to the manufacturer’s protocols applied to the prepared cDNA samples ...
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bioRxiv - Cancer Biology 2019Quote: ... and was run on miScript miRNA PCR Array Human Ovarian Cancer plates (Qiagen, MIHS-110ZE-4, 384 well plate). PCR plates were read the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... miScript miRNA PCR array of Human Neurological Development and Disease was performed using miScript SYBR green PCR kit (Qiagen). The PCR array contains 84 miRNAs which are differentially expressed during neuronal development and are responsible for progression of neurological diseases.
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bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: The human VCP cDNA was reverse transcribed from total RNA by using Omniscript RT kit (Qiagen Japan, Tokyo, Japan) extracted from A549 cells (RIKEN Cell Bank ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA targets were only included if biochemically confirmed using human tissue or non-species specific methods (sourced from QIAGEN’s curated Ingenuity Knowledge Base[48] ...
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bioRxiv - Immunology 2021Quote: ... followed by amplification and quantification using RT2 Profiler Human Innate and Adaptive Immune Response 96-well Array (Qiagen, 330231) with RT2 SYBR Green ROX qPCR Mastermix (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequenced reads were mapped with the hg38 human genome and sequence analysis was done by the CLC genomics workbench (12.0.3, Qiagen Bioinformatics). Additionally ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from both NHP and human stool samples using the PowerFecal DNA Isolation Kit (Qiagen, Valencia, CA). Sequencing of the 16s small subunit ribosomal ribonucleic acid (SSU rRNA ...
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bioRxiv - Physiology 2024Quote: RNA was extracted from human tissue derived organoids and neutrophils using the RNeasy® Plus Mini Kit (Qiagen, #74104) with cDNA prepared using a SuperScript™ VILO™ cDNA synthesis kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Human DNA from whole cell extracts of A549 cells was purified using the DNeasy Blood and Tissue kit (Qiagen). RNAseA (Thermo ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from human tissue and cyst fluid using the QIAamp Mini kit (Qiagen, Hilden, Germany). Extractions were performed per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Raw FASTQ reads were aligned to the GRCh38 human genome using CLC Genomics Workbench version 22.0.2 software (Qiagen, USA). Differential gene expression was determined using the built-in tool in CLC Genomics workbench ...
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bioRxiv - Genetics 2024Quote: Total RNA from post-mortem adult human neural retina was extracted following manufacturer’s guidelines (RNeasy Mini kit®, Qiagen) followed by DNase treatment (ArcticZymes ...
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bioRxiv - Molecular Biology 2024Quote: ... as per the manufacturer’s instructions and profiled the most abundant 1092 miRNAs using human mirnome miscript miRNA PCR array (Qiagen). The data was analysed by ΔΔCT method of relative quantification using an analysis tool available at geneglobe.qiagen.com.
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bioRxiv - Microbiology 2024Quote: The RNAseq data were mapped to the human reference genome (GRCh38.109) using CLC Genomics Workbench 21.0 (QIAGEN, Aarhus, Denmark). The Voroni diagram was created using Python 3.11 and matplotkib 3.7.2 ...
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bioRxiv - Developmental Biology 2020Quote: ... 100 μl of chloroform was added and mixed vigorously and the aqueous component was isolated using MaXtract High Density tubes (Qiagen, 129046) using the manufacturers protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Bornean orangutan (AG05252, Coriell Institute), and Sumatran orangutan (AG06213, Coriell Institute) using the MagAttract High Molecular Weight DNA Kit (Qiagen, Germany). Male chimpanzee DNA sample (CH159 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the magnetic beads were washed in high salt buffer and RNA was extracted with the RNeasy Micro kit (Qiagen: 74004). For RNA sequencing ...
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bioRxiv - Biophysics 2021Quote: ... A small volume of this reaction was then used to seed a PCR reaction using primers prAH869 and prAH870 in Q5 High-Fidelity 2X Master Mix (Qiagen M0492). Products were purified by RNAClean XP magnetic beads (Beckman-Coulter A63987 ...
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bioRxiv - Microbiology 2022Quote: Complete genome of strain YC6860T was sequenced by extracting its high-quality genomic DNA with the blood and cell culture DNA minikit (Qiagen, MD). The genome of YC6860T was sequenced and characterized using Illumina GAIIx sequencer (San Diego ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High molecular weight genomic DNA (gDNA) was isolated from each coral tissue sample using the Qiagen DNeasy kit (Qiagen, Valencia, CA) with slight modifications described previously (dx.doi.org/10.17504/protocols.io.bgjqjumw) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... high quality DNA was extracted from flash frozen placental tissue using a DNeasy Blood and Tissue Kit (Qiagen, cat. no. 69506), and bisulfite converted with the Zymo EZ DNA Methylation Gold spin column kit (cat ...
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bioRxiv - Synthetic Biology 2023Quote: ... The recombinant plasmid was then purified at a high concentration (200 μg/mL) using the plasmid preparation kit (Qiagen, Germantown, MD).
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bioRxiv - Genomics 2024Quote: ... muscle samples were used to obtain high molecular weight gDNA using the QIAGEN MagAttract HMW DNA kit (QIAGEN, Germantown, MD, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: We extracted high molecular weight DNA from approximately 20 mg cortex tissue from each donor using the MagAttract HMW DNA kit (Qiagen 67563). We prepared linked read libraries using the Chromium Genome Reagent Kit v2 following the protocol provided by 10x Genomics ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) analysis were carried out on cDNAs retrotranscribed with High Capacity cDNA RT kit (Qiagen - 4368814), and analyzed genes were amplified using 4x Capital qPCR Green Master mix (BiotechRabbit - BR0501702 ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified using a Ni-NTA Superflow column (Qiagen, Netherlands, 30761) on a Äkta Express fast protein liquid chromatography system (FPLC ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transiently transfected with mammalian protein expressing plasmids using Effectene (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... RNA attached to purified protein complexes was isolated with the miRNeasy kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: DNA was extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Expressed proteins were subsequently purified by Ni-chelate chromatography (Qiagen, 5 ml column) and SEC (S75 ...
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bioRxiv - Biochemistry 2020Quote: ... and the soluble protein fractions were purified by Ni-NTA resin (Qiagen, # 30230). The purified fraction was concentrated ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified with nickel chelate affinity resins (Ni-NTA, Qiagen). The protein samples were collected and purified by a Superdex 200 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen), amylose (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Microbiology 2021Quote: ... Whole DNA and proteins were extracted by the ALLprep Kit (Qiagen, Hilden, Germany), following manufacturer instructions.